BCIP/NBT is a widely used set of chromogenic substrates for the enzyme label alkaline phosphatase (AP). Once catalyzed by AP, the product of BCIP (5-Bromo-4-chloro-3-indolyl phosphate) further reacts with NBT (nitro blue tetrazolium) to produce an insoluble precipitate that is dark blue to purple in color. BCIP/NBT is useful in detecting protein targets in applications such as immunohistochemistry and western blotting.
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