BCIP/NBT

BCIP/NBT is a widely used set of chromogenic substrates for the enzyme label alkaline phosphatase (AP). Once catalyzed by AP, the product of BCIP (5-Bromo-4-chloro-3-indolyl phosphate) further reacts with NBT (nitro blue tetrazolium) to produce an insoluble precipitate that is dark blue to purple in color. BCIP/NBT is useful in detecting protein targets in applications such as immunohistochemistry and western blotting.

BCIP/NBT

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Alkaline Phosphatase chromogen (BCIP/TNBT)
  • Quantity:
    100 ml
  • Applications:
    Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(1)
BCIP disodium salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 MG, 500 MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
BCIP toluidine salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 MG, 500 MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen
  • Quantity:
    100 ml
  • Applications:
    Dot Blot, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(4)
Nitrotetrazolium blue chloride (NBT), Chromogenic substrate
  • Quantity:
    1 G, 5 G, 250 MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(4)
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