Recombinant Protein Purification

Recombinant protein purification kits and reagents are available in a variety of formats to suit different types of affinity tags and processing scales. Purification methods typically proceed using solid supports bound to tag-specific ligands, including nickel and cobalt (histidine or 6-his tag), glutathione (GST-tag), and antibodies to specific epitopes (DYKDDDDK, HA-tag, Myc). The choice of support can also vary depending on the scale or desired method of purification, with magnetic beads being favorable for quick, small-scale purifications, and agarose resins accommodating a wider range of process scales. In choosing a tagged protein purification kit, also consider the expression system cell type and the intended downstream application.