While most protein extraction procedures begin with cellular lysis to produce a crude extract, methodologies and reagents will vary depending on the application. Denaturing protein electrophoresis experiments as SDS-PAGE and Western blots typically employ strong detergents and solution conditions that ensure protein solubility. On the other hand, protein extraction for biochemical analyses often requires gentler reagents to ensure retention of non-denatured protein activity. Purification of native proteins will likely require additional steps, such as subcellular and organelle fractionation, when compared to tagged recombinant proteins. Commercial protein extraction kits are available from various sources, containing ready-to-use reagents for convenient and optimized protein preparation.
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Sponsored Products
- 1 Kit
- Purification, Cell Fractionation, Cell Lysis, Protein Extraction, Protein Purification
- Nuclear Protein
- 100 Tests
- Purification, Cell Fractionation, Cell Lysis, Protein Extraction, Protein Purification
- Membrane Protein
- 100 Tests
- Purification, Cell Fractionation, Cell Lysis, Protein Extraction, Protein Purification
- Nuclear Protein
- 50 Tests
- Cell Fractionation, Cell Lysis, Protein Extraction, Protein Purification
- Nuclear Protein
- 100 Preps
- Cell Fractionation, Protein Extraction, Protein Purification
- Nuclear Protein
- 50 Tests
- Protein Extraction
- Nuclear Protein
- 20 Tests
- Protein Extraction
- Nuclear Protein
- 20 Preps
- Cell Fractionation, Protein Extraction, Protein Purification
- Nuclear Protein
- 50 Assays
- Protein Extraction, Protein Purification
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