EVOS™ M5000 Imaging System from Thermo Fisher Scientific

Product Specs
ItemEVOS™ M5000 Imaging System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberAMF5000
Quantity1 system
TypeSemi-automated Digital Microscope
ApplicationsFluorescent and colorimetric imaging, Z-stack capability, time-lapse imaging, and single-click multichannel capture
Light SourceLED
Dimensions18 x 18 x 23 inches
Image ModeFluorescence, brightfield, color brightfield, phase contrast

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Jujuboside A induces bladder cancer cell apoptosis by inhibiting ATP1A2-mediated mitochondrial energy metabolism regulationCancer Biology & TherapyDecember 31, 2026Meng Zhu, Yuepeng Liu, Yumin Jia, Lixin Ren, Shuhui An, Yaxuan Wangcells were washed 2–3 times with PBS and observed and photographed using a fluorescence microscope (AMF5000; Thermo Fisher Scientific). Changes in fluorescence intensity were analyzed using the ImageJ 1.5.2a

(2) Effects of dietary supplementation with polyphenolic extracts from Lasia spinosa Thw. roots on reproductive performance in Guangxi Ma RoostersPoultry ScienceJune 1, 2026Lang Zhang, Zhenlin Chen, Ying Zhang, Maosen Yang, Liping Pu, Haichuan Tan, Huiyan Xu, Mingxia Ran, Xingting Liu, Yangqing Luperformed with reagents purchased from Solarbio (Beijing, China); 6. Digital imaging was conducted using an EVOS™ M5000 Imaging System (Thermo Fisher Scientific, USA), and seminiferous tubule diameter and spermatogenic epithelial height

(3) Synergistic Effects of IMP-1700, Ciprofloxacin, and X-Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial-Resistant BacteriaMicrobiologyopenApril 27, 2026Ida Vang Andersen, Ane Beth Sloth, Emilie Caroline Skuladottir Bøgestad, Christian Bernard Matthijs Poulie, Anne Skovsbo Clausen, Umberto Maria Battisti, Matthias M. Herth, Andreas Kjaerdetermine any changes in cell confluency and morphology, images were acquired for each treatment using the EVOS M5000 Imaging System with a 10X objective (Thermo Fisher). To quantify the cells and their viability after treatment, they

(4) In vitro and in vivo anti-piroplasm activity of PBT2 against Theileria annulata and Babesia microtiInt J Parasitol Drugs Drug ResistApril 22, 2026Jin Che, Yixuan Wu, Junwei Wang, Yijun Chai, Jinming Wang, Wei Li, Shuaiyang Zhao, Guiquan Guan, Hong Yin(Cheeseman et al., 2021) and host nuclei was stained with DAPI. Images were captured and analyzed using EVOS M5000 Imaging System (ThermoFisher Scientific). 2.4 Cell viability and proliferation assay in vitro TaNM, TaXJS and BoMac

(5) CDKN1A/p21 Influences the Survival and Expansion of Breast Cancer Stem Cells after Oxidative DamageOncology ResearchApril 22, 2026Evangelos Manousakis, Cristina Moreta-Moraleda, Clàudia Martinez Miralles, Anna Tomàs Tomàs Pujolà, Houda Baccara, Laia Liñán Franquet, Montserrat Montañés Montañés Albó, Roberto Ferrari, Roni H. G. Wrightthe images were captured via the Invitrogen™ EVOS™ M5000 Imaging System (Thermo Fisher Scientific, #AMF5000). The spheroid number and size were quantified with ImageJ (Version 1.53t, National Institutes of Health

(6) Utilizing Caenorhabditis elegans as an In Vivo Model for Identifying Small Molecules Modulating Lipid Metabolism: A GuidelinePrev Nutr Food SciApril 20, 2026Zixuan Teng, Aaron Taehwan Kim, Peiyi Shen, Soonkyu Chung, Yeonhwa Parkusing a Nikon SMZ745 Stereo Microscope (Nikon Instruments Inc.) at 50× magnification for A and B and an EVOS M5000 Imaging System (Invitrogen, Thermo Fisher Scientific) at 40× magnification for C.

(7) The Antidepressant Amitriptyline Upregulates ERK1/2 Signaling and Inhibits Rho-Mediated Responses Induced by Lysophosphatidic Acid in Astroglial CellsInt J Mol SciApril 20, 2026Maria C. Olianas, Simona Dedoni, Pierluigi OnaliUSA). Cell nuclei were stained with 0.1 µg/mL DAPI. Fluorescence staining was visualized by using an EVOS M5000 imaging system (Invitrogen/Thermo Fisher Scientific) equipped with light cubes for DAPI (AMEP4950), GFP (AMEP4951)

(8) Ultrasound-Generated Nanoscale Mechanical Stimulation to Regulate Stem Cell DifferentiationACS Nanoscience AuApril 15, 2026Siddhesh Saigaonkar, Aditi Joshi, Akshay Kumar, Arka Roy Choudhury, Rudra Pratap, Ajay Tijorewashing with PBS, coverslips were mounted on glass slides, and images were captured at 10× using the Evos M5000 imaging system (Invitrogen). Images were analyzed using ImageJ software. Graphs were made using GraphPad Prism 8.4. 5.5

(9) The Preclinical Effects and Mechanisms of Biofield Therapy on Pancreatic Cancer Cell Growth and MetastasisCancer MedApril 13, 2026Peiying Yang, Daoyan Wei, Sharmistha Chakraborty, Phuong Nguyen, Andrew Cusimano, Defeng Deng, Shafaqmuhammad Iqbal, Monica Nelson, Meng Cui, Jianliang Dai, et al.at 37°C. Green fluorescent protein and DAPI images were captured by the EVOS M5000 Imaging System (AMF5000) or EVOS M7000 Imaging System (AMF7000). Fluorescence intensity was quantified by ImageJ for the images

(10) Integrin-Binding Matricellular Protein Fibulin-5 Maintains Epidermal Stem Cell Heterogeneity During Skin AgingAging CellApril 10, 2026Wenxin Fan, Mizuho Ishikawa, Erna Raja, Ahmed M. Hegazy, Hinata Date, Yen Xuan Ngo, Yoshifumi Sato, Kazuya Yamagata, Hiromi Yanagisawa, Aiko Sadabefore dehydration and mounting in Entellan solution (Merck Millipore). Images were captured using an EVOS M5000 Imaging System (Thermo Fisher Scientific). Skin sections were immunostained as previously described (Sada et al. 2016).

(11) Expression profiling of inhibitory immune checkpoints in colorectal cancer stem cells and their association with tumor immunity and immunotherapy biomarkersFront ImmunolApril 8, 2026Ola J. Hussein, Muhammad Ammar Zahid, Hanan H. Abunada, Abdelali Agouni, Cristina Maccalli, Hesham M. KorashyMountant (Invitrogen, cat. P36984). Slides were protected from light and were examined and imaged on an EVOS™ M5000 Imaging System (Invitrogen) using identical exposure settings across groups. 2.8 siRNA knockdown Specific siRNAs

(12) Defining the RNA Modification Landscape of Multiple Myeloma Reveals METTL3-Dependent m 6 A Regulation of NEAT1bioRxivApril 7, 2026Prasanth Thunuguntla, Dhanusha Duraiyan, Catheryn Sizemore, Elizabeth Sulvaran-Guel, Richa Mishra, Jessica Camacho, Savannah Gonzales, Stephen Daly, Katelyn Bagwill, Dakota Colbert, et al.D9542), mounted using ProLong Gold Antifade Mountant (Invitrogen; catalog no. P36930), and imaged on an EVOS M5000 Imaging System (Invitrogen). RNA spot counts per cell and subcellular distribution were quantified using QuPath v0.5.1

(13) Antimicrobial efficacy and cytotoxic assessment of plasma-activated water generated by a dielectric barrier discharge microbubble systemFront MicrobiolApril 1, 2026Raquel Oliveira Ferreira, Danielle C. F. S. Spigarollo, Iolanda Cristina Silveira Duarte, Elaine C. de Oliveira, Nilson Cristino da Cruzperformed at the National Institute of Photonics Applied to Cellular Biology (INFABIC, UNICAMP) using an EVOS M5000 Imaging System (Thermo Fisher Scientific) with a 20 × objective. Images were processed using ImageJ FIJI software with

(14) Doxorubicin enhances adipogenesis in an FGF2-dependent manner and induces a tumour-promoting secretory phenotypeJournal of Bone OncologyApril 1, 2026Lauren M. Kreps, Edward Yakubovich, Huijun Zhao, Selam Yimer, Edward Yang, Juliet Cruz, Barbara Vanderhyden, Christina L. Addisonsolution to a final concentration of 3.8 nM. Stained cells were washed 3x with PBS and then imaged using EVOS M5000 Imaging System (Invitrogen, Waltham, MA). All steps were performed at room temperature. Percentage of BODIPY positive

(15) Effects of illite or bentonite on cytotoxicity, antibacterial and adsorption capacity in porcine intestinal epithelial cellsJournal of Animal Science and TechnologyMarch 31, 2026Seyeon Chang, Jihwan Lee, Seungwon Jung, Dongcheol Song, Sehyun Park, Kyeongho Jeon, Hyuck Kim, Jinho Chodebris through three DPBS washes, the area of the scratch was measured under an inverted microscope (EVOS M5000 Imaging System, Thermo Fisher Scientific). After 12 and 24 hours, the change in the area of the scratch was measured. Cell

(16) Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid SynthesisbioRxivMarch 31, 2026Sage W. R. Geher, Seyi Falekun, Jessica N. Pita-Aquino, Russell P. Swift, Megan Okada, Yasaman Jami-Alahmadi, James A. Wohlschlegel, Sean T. Prigge, Paul A. Sigala2° antibody (Invitrogen A32731). Images of aACP knockdown parasites (Dd2 and PfMev) were taken on an EVOS M5000 imaging system, with processing done in Fiji/ImageJ. Images of ΔACPS, ΔACP, and ΔFabD parasites were acquired on a

(17) Induction of caspase-mediated suppression of interferon signaling potentiates viral oncolysis of resistant tumor cellsCell Communication and SignalingMarch 24, 2026Vladimir Bozhdansky, Shelly Tayar, Shlomi Kashkash, Eran Bacharach, Marcelo Ehrlichpost-infection/treatment, spheroids were stained with Hoechst at a final concentration of 10 µg/ml and imaged using an EVOS™ M5000 Imaging System (Thermo Fisher Scientific) at 20x magnification. Fluorescent microscopy Cells were plated at a density

(18) KNL1 Regulates Ferroptosis Resistance and Migration in Lung Adenocarcinoma Cells via AMPK-mTOR SignalingOncology ResearchMarch 23, 2026Yiran Dong, Jingyue Wang, Jiayang Chen, Liang Mo, Yong Youwas then placed in a 37°C, 5% CO2 incubator for 30 min. Fluorescence signals were acquired using an EVOS M5000 Imaging System (Thermo Fisher Scientific) with green excitation light (Ex: 532 nm/Em: ~580 nm). All Fe2+ detection

(19) Primary Cilia Are Required for Efficient BMP Signaling in Traumatic Heterotopic OssificationBiomedicinesMarch 19, 2026Xinyuan Yuan, Saman Toutounchi, Susan F. Law, David Achudhan, Abhishek Chandra, Kai He, Yingshu Cao, Jinghua Hu, Robert J. Pignolo, Haitao Wangapproximately 80% confluence. Transduction efficiency was assessed using a fluorescence microscope (EVOS M5000 Imaging System; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). Following transduction, Ift88-deficient cells

(20) Simtuzumab Attenuates Loxl2-Mediated Extracellular Matrix Remodeling and Preserves Cardiac Function in LMNA Mutation-Induced Dilated CardiomyopathyCirculation: Heart FailureMarch 17, 2026Marie Kervella, Charlotta S. Behrens, Cécile Peccate, Zoheir Guesmia, Fiorella Grandi, Nathalie Mougenot, Anne Forand, Azzouz Charrabi, Guy Brochier, Ramaroson Andriantsitohaina, et al.Immunofluorescence microscopy was performed using an EVOS M5000 microscope (Invitrogen, catalog no. AMF5000), a fully inverted imaging system equipped with 4-color fluorescence, transmitted light, and imaging

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11/1/2022Awesome Microscope Dimi ComfuOur group is interested in CD8 T-cells and their applications in immunotherapy. Most of our assays are in-vitro cell-based, so microscopy is commonly used.