Description
The Eagle Biosciences Mouse/Rat IGFBP-2 ELISA, is a so-called sandwich-assay. It utilizes two different specific high affinity polyclonal antibodies for this protein. The IGFBP-2 in the samples binds quantitatively to the immobilized antibody. In the following step, the biotinylated antibody in turn binds IGFBP-2. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antibody. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the IGFBP-2 content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption