Fig 1: Validation of the kinases - Mitogen-activated protein kinase 3 (MAPK3) and Cyclin-dependent kinase 1 (CDK1). (A) Western Blotting analysis of the Thr564 site in MAPK3 in the embryogenesis of worker and drone at 24 h. GAPDH normalized the relative expression values of the target peptide in the two bees. (B) Western Blotting analysis of the Tyr15 in CDK1 in embryogenesis of worker and drone at 48 h. (C) The enzymatic activity of MAPK3 in embryogenesis of worker and drone at 24 h. (D) The enzymatic activity of CDK1 in embryogenesis of worker and drone at 48 h. The asterisk indicates a significant difference at p < 0.05.
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