GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) ab205811 from Abcam

Product Specs
ItemGSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab205811
Quantity100 Test
Sample TypeUrine, Plasma, Tissue Extracts, Cell Lysate
SensitivityEqual to 10 nM
Assay Time1h 0m
Research AreaMetabolism

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(1) The Ca2+ sensor STIM1 promotes neuronal ferroptosis by regulating iron homeostasis to exacerbate brain injury after intracerebral hemorrhageCell Reports MedicineFebruary 17, 2026Hongchen Zhang, Lijun Ma, Zheming Yue, Chuanhao Lu, li Wang, Min Zhang, Jia Yong, Yuan Feng, Shiquan Wang, Hongjie Wang, et al.AAT Bioquest, USA). The GSH and GSSG levels were measured using a GSH/GSSG ratio detection assay kit (ab205811, Abcam, UK). The GPX4 activity was measured using the GPX4 activity assay kit (E-BC-K883-M, Elabscience,

(2) Taming the Immiscibility of Gold, Iron, and Boron to Craft Chemodegradable Nanoparticles for Multimodal Imaging and RadiotherapyAdvanced Healthcare MaterialsFebruary 15, 2026Michael Bissoli, Clara M.G. de Faria, Veronica Torresan, Maria Assunta Lacavalla, Mattia Cattelan, Denis Badocco, Paolo Pastore, Pasquina Marzola, Laura Cansolino, Cinzia Ferrari, et al.groups. For the GSH/GSSG assay using the GSH/GSSG Ratio Detection Assay Kit II – Fluorometric (Green) (ab205811, Abcam, UK), 6 × 103 cells per well were seeded into black‐wall 96‐well plates with clear bottoms and

(3) IGF2BP3-dependent glutamine/BCAA metabolic rewiring rejuvenates aged human adipose-derived stem cells for enhanced tissue regenerationCell DiscoveryJanuary 20, 2026Zichao Li, Lin Feng, Xinxin Wei, Huichen Li, Yifu Zhu, Hongtao Wang, Jiaqi Liu, Liang Luo, Zhao Zheng, Baoqiang Song, et al.medium. Intracellular GSH, glutamate, succinate, fumarate, and α-KG levels were determined using GSH (#ab205811; Abcam), glutamate (#ab83389; Abcam), succinate (#ab204718; Abcam), fumarate (#ab102516; Abcam), and

(4) N-acetylcysteine amide alleviates pathological neovascularization through mitochondrial protection and energy metabolism reprogramming of vascular endothelial cellsRedox BiologyDecember 1, 2025Jicheng Lin, Fengmei Yang, Andina Hu, Xiaoyu Tang, Likun Lin, Peiqi Wu, Haolin Kong, Changxuan Liu, Kaixuan Cui, Boyu Yang, et al.GSH/GSSG ratio in HRMECs were measured using the GSH/GSSG Ratio Detection assay kit (fluorometric-green; ab205811, Abcam) following the manufacturer's instructions. 2.9 TOMM20 staining and super-resolution structured

(5) A niche-dependent redox rheostat regulates epithelial stem cell fateNature CommunicationsNovember 28, 2025Xi Chen, Krishnan Raghunathan, Bin Bao, Elsy Ngwa, Andrew Kwong, Zhongyang Wu, Stephen Babcock, Clara Baek, George Ye, Anoohya Muppirala, et al.ab204708), we then ran GSH and Total Glutathione assay following the manufacturer’s instructions (Abcam, ab205811). Gel electrophoresis and immunoblot analysis After recovered from Matrigel, colonoids were washed

(6) Inducing ferroptosis to impede metastasis by inhibiting the calcium channel TRPC6Cell ReportsNovember 25, 2025Boris S. Dimitrov, Dimpi Mukhopadhyay, Hira Lal Goel, Emmet R. Karner, Christi A. Silva, Ayush Kumar, Cornelia Peterson, Mengdie Wang, Arthur M. Mercurio(M-PER Mammalian Protein Extraction Reagent, Cat# 78501, Thermo Fisher). The GSH/GSSG Ratio Detection Assay Kit (Abcam, Cat# ab205811) was used to determine total GSH levels based on linear regression analysis of the GSH standard curve. Clinical

(7) SLC16A3 drives lung adenocarcinoma progression and gefitinib resistance through coordinated regulation of ferroptosis and lactate metabolismFrontiers in ImmunologyNovember 10, 2025Wenhan Cai, Yiming Liu, Kai Zhao, Zirui Zhu, Jiamei Jin, Jiaxin Wen, Zhiqiang Xueintracellular NADPH/NADP+ and GSH/GSSG ratios were quantified using commercial assay kits (ab65349; ab205811, Abcam, USA) according to the manufacturer’s instructions. Briefly, cells were lysed, deproteinized,

(8) Effects of low intracellular glutathione and CbrA-CbrB-Crc signaling on methylglyoxal sensitivity in Pseudomonas aeruginosa LasR-deficient mutantsJournal of BacteriologyOctober 23, 2025Ana Altamirano, Marina Ruzic, Anna E. Cryan, Deborah A. Hogandrum for 16 h and then adjusted to an OD600 of 1.0. The GSH/GSSG ratio detection assay kit II (Abcam #ab205811) was used to measure intracellular GSH. The manufacturer’s protocol was adapted for use in Pseudomonas aeruginosa

(9) Mitochondrial calcium uniporter promotes MSU crystal-induced inflammation through inducing mitochondrial Ca2+ overload and ubiquitination of SIRT5 proteinArthritis Res TherAugust 22, 2025Qingqing Yu, Renjie Li, Guizhao Yang, Yan Xiao, Xingyu Liu, Yaxin Deng, Xiongyan Luo, Qian Dai, Mei ZengAssay Kit, S0051, Beyotime Biotechnology, China) as well as GSH/GSSG ratio (GSH/GSSG Ratio Assay Kit, ab205811, Abcam) were assayed following the manufacturer’s instructions. DIA proteomics and bioinformatics analysis Through

(10) Androgens drive SLC1A5-dependent metabolic reprogramming in polycystic ovary syndromeNat CommunAugust 15, 2025Yishu Wang, Jiaying Wu, Gaochen Zhang, Yan Shi, Yicong Meng, Pingping Lv, Weiwei Huang, Yunfei Su, Zhiyang Zhou, Bo Wang, et al.ratio of reduced GSH to oxidized glutathione (GSSG) was measured by the GSH/GSSH assay kit (Abcam, ab205811) with the fluorescence (Ex/Em: 490/520 nm) recorded, following the manufacturer’s instructions. Changes

(11) GPx3 marks adipocyte lineage commitment in bone marrow stromal cellsJ Orthop Surg ResMay 23, 2025Zhongxiang Wang, Yangyang Hu, Mao Li, Xiaojun Chen, Chengyu Zhou, Zhiyang Xu, Kai Chen, Lichuang Wureproducibility. GSH and GSSG detection The GSH/GSSG ratio was detected by GSH/GSSG Ratio Detection Assay Kit (Abcam, ab205811) according to the manufacturer’s instructions. In brief, cells after 5 days of adipogenic induction

(12) Covalent Binding of Thioredoxin to TXNIP is Required for Diet-induced Insulin Resistance in the LiverJournal of Biological ChemistryMay 8, 2025Sezin Dagdeviren, Megan F. Hoang, Jialu Wang, Tamara Goldberger, Amelia M. Yu, Steven J. Blair, Jake C. Benoit, Elisabeth M. Ricci-Blair, Veronika Y. Melnik, Bo-Yeon Kim, et al.#ab83390, #ab272541). Liver GSH/GSSG values were measured using GSH/GSSG Ratio Detection Kit II (Abcam; #ab205811). Western blots were performed using Phospho-Akt (Ser473) (#9271), Akt (#9272), Beta-actin (#8457),

(13) Hippo-YAP/TAZ-ROS signaling axis regulates metaflammation induced by SelenoM deficiency in high-fat diet-derived obesityJournal of Advanced ResearchMay 1, 2025Jingzeng Cai, Jiaqiang Huang, Di Li, Xintong Zhang, Bendong Shi, Qiaohan Liu, Cheng Fang, Shiwen Xu, Ziwei Zhangsuperoxide dismutase (SOD, S0087, Beyotime), total antioxidant capacity (T-AOC, S0119, Beyotime), GSH/GSSG (ab205811, Abcam, Cambridge, UK) and NADPH/NADP+ ratios (AAT-15259, Amyjet Scientific Co., Ltd., Wuhan, China).

(14) Hepatotoxicity evaluation method through multiple-factor analysis using human pluripotent stem cell derived hepatic organoidsSci RepMarch 28, 2025Dae-Seop Shin, Jung Yoon Yang, Ha Neul Jeong, Seon Ju Mun, Hyunwoo Kim, Myung Jin Son, Myung Ae BaeGSH/GSSG ratio The GSH/GSSG ratio was measured using the GSH/GSSG Ratio Detection Assay Kit II (Abcam, #ab205811) according to the manufacturer’s instructions. Briefly, after treatment with the hepatotoxic compounds

(15) Helium cold atmospheric pressure plasma reduces erastin induced inflammation and ferroptosis in human gingival fibroblastsSci RepMarch 3, 2025Jung Sun Park, Young-Il Jeong, Byung-Hoon Kim(NaHCO3). Total and reduced GSH levels were analyzed using the GSH/GSSG Ratio Detection Assay Kit II (ab205811, Abcam, Cambridge, MA, USA) according to the manufacturer’s instructions and a fluorescence-monitoring

(16) Polydatin-Induced Shift of Redox Balance and Its Anti-Cancer Impact on Human Osteosarcoma CellsCurrent issues in molecular biologyDecember 31, 2024Cimmino A, Gioia M, Clementi ME, Faraoni I, Marini S, Ciaccio C.DMSO-treated cells. 2.8. Glutathione (GSH) Assay The GSH/GSSG Ratio Detection Assay Kit II (Abcam, ab205811, Cambridge, UK) was used to detect reduced (GSH) and oxidized (GSSG) glutathione levels, following the

(17) JP4-039 Mitigates Cisplatin-Induced Acute Kidney Injury by Inhibiting Oxidative Stress and Blocking Apoptosis and Ferroptosis in MiceAntioxidants (Basel)December 15, 2024Merlin Airik, Kacian Clayton, Peter Wipf, Rannar Airikthen assayed for total glutathione (GSH) content according to the manufacturer’s instructions (Abcam, ab205811, Cambridge, UK). The protein levels were determined using the DC Protein Assay Kit (Bio-Rad). Values

(18) Therapeutic induction of ferroptosis in tumors using PD-L1 targeting antibody nanogel conjugatesCell Chemical BiologyDecember 1, 2024Mengdie Wang, Theeraphop Prachyathipsakul, Christi A. Wisniewski, Choua Xiong, Shivam Goel, Hira Lal Goel, Emmet R. Karner, Dimpi Mukhopadhyay, Prachi Gupta, Aniket Majee, et al.(M-PER Mammalian Protein Extraction Reagent, Cat. No. 78501, Thermo Scientific). The GSH/GSSG ratio detection kit II (Cat. No. ab205811; Abcam) was used to determine the levels of GSH+GSSG concentration in each sample, as determined by

(19) Deletion of CD38 mitigates the severity of NEC in experimental settings by modulating macrophage-mediated inflammationRedox BiologyNovember 1, 2024Yue Ma, Yunfei Zhang, Xinli Liu, Xinyi Yang, Hongjie Guo, Xionghui Ding, Cuilian Ye, Chunbao GuoGSSG was conducted as per established methods [41], utilizing the GSH/GSSG Ratio Detection Assay Kit (ab205811, Abcam, Cambridge, UK). The isolated, snap-frozen intestinal tissues were homogenized and centrifuged

(20) HJURP inhibits sensitivity to ferroptosis inducers in prostate cancer cells by enhancing the peroxidase activity of PRDX1Redox BiologyNovember 1, 2024Wenjie Lai, Weian Zhu, Jianjie Wu, Jiongduan Huang, Xiaojuan Li, Yun Luo, Yu Wang, Hengda Zeng, Mingqiang Li, Xiaofu Qiu, et al.absorbance of each sample was measured at 593 nm. GSH assay: GSH/GSSG Ratio Detection Assay Kit II (Abcam, ab205811) was used to determine the GSH content in C4-2 and PC3 cells. Based on the manufacturer's protocol,

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11/15/2021Good for GSH/GSSG Ratio Detection Assay Kit Kumar SubramaniAn imbalance in the antioxidant network through the enhanced generation of ROS or depletion of antioxidants leads to oxidative stress. Sulfhydryl-rich molecules play a critical role in the antioxidant... Read Review

GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) from Abcam

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GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) from Abcam

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