Deproteinizing Sample Preparation Kit - TCA ab204708 from Abcam

Product Specs
ItemDeproteinizing Sample Preparation Kit - TCA
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204708
Sample TypeUrine, Whole Blood, Tissue Extracts, Tissue Culture Media, Tissue Lysate
Assay Time0h 25m
Quantity200 Test

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Hypertension Drives Protein Lactylation and Vascular Dysfunction in Skeletal MuscleHypertensionApril 22, 2026Milene T. Fontes, Paul Townsend, Landon Butler, Juliana M. Parente, Fênix A. Araujo, Tiago J. Costa, Gisele F. Bomfim, Laena Pernomian, Wenbin Tan, Brandon N. VanderVeen, et al.for plasma). Muscle samples were deproteinized using the Deproteinizing Sample Preparation Kit-TCA (ab204708, Abcam). Protein concentration was determined using the BCA Protein Assay Kit (ThermoFisher Scientific,

(2) Hypertension Drives Protein Lactylation and Vascular Dysfunction in Skeletal MuscleHypertensionApril 22, 2026Milene T. Fontes, Paul Townsend, Landon Butler, Juliana M. Parente, Fênix A. Araujo, Tiago J. Costa, Gisele F. Bomfim, Laena Pernomian, Wenbin Tan, Brandon N. VanderVeen, et al.for plasma). Muscle samples were deproteinized using the Deproteinizing Sample Preparation Kit-TCA (ab204708, Abcam). Protein concentration was determined using the BCA Protein Assay Kit (ThermoFisher Scientific,

(3) ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinomaCell DiscovApril 21, 2026Lijie Han, Lina Gan, Balázs Schäfer, Dillon M. Voss, Mathias Danielsen, Ondrej Kostov, Jia Liu, Ting Wang, Marvin H. Caruthers, Hao Chen, et al.assay buffer. The lysates were deproteinized using a Deproteinizing Sample Preparation Kit (Abcam, ab204708). Flow cytometry Cells (5 × 104 cells/well) were seeded in six-well plates and treated with 20 μM

(4) MALAT1 Regulates Human Macrophage Metabolism by Interacting with HADHBiScienceMarch 20, 2026Yuxiang Liu, Yukiteru Nakayama, Junichi Sugita, Tsukasa Oshima, Kunihito Kani, Atsushi Kobayashi, Naoto Setoguchi, Yoshiko Iwai, Ichiro Manabe, Katsuhito FujiuE7770L L-lactate Assay Kit (Colorimetric),Abcam,Cat# ab65331 Deproteinizing Sample Preparation Kit-TCA,Abcam,Cat# ab204708 MycoFluor Mycoplasma Detection Kit,Thermo Fisher,Cat# M7006 ,, Deposited data,Deposited data,Deposited

(5) Assessment of the angiogenic potential of xenografted tumors by biomedical imaging techniquesBiomedical Optics ExpressMarch 1, 2026Irina Druzhkova, Anna Orlova, Pavel Subochev, Alexey Kurnikov, Anna Glyavina, Alina Isakova, Ekaterina Kukovyakina, Ekaterina Plotnikova, Marine Gasparian, Anastasia Komarova, et al.24 h. Then the culture medium was collected and proteins were removed using a deproteinization kit (ab204708, Abcam, USA). Samples were analyzed using a colorimetric lactate assay kit (ab65331, Abcam, USA) and

(6) Disrupted energy metabolism is associated with retinal ganglion cell degeneration in autosomal dominant optic atrophyScience AdvancesFebruary 20, 2026Eugene Yu-Chuan Kang, Yun-Ju Tseng, Wei-Hao Peng, Hui-Chuan Hung, Pei-Hsuan Lin, Katrina P. Montales, Emmet Sherman, John Peregrin, Ethan Hunghsi Wang, Chunya Kang, et al.analysis. To prevent enzyme interference in the assay, deproteinization was performed using a kit (ab204708, Deproteinizing Sample Preparation Kit, Abcam). After a 30-min incubation, the ATP assay was conducted,

(7) miR-940 suppresses ferroptosis by controlling expression of key regulatory genesbioRxivFebruary 14, 2026Andrea Kolak, Juliane Tschuck, Stefanie A. I. Weiß, Daniel Kaemena, Karolin Klimm, Ana Galhoz, Larissa Ringelstetter, Myles Fennell, Juliane Merl-Pham, Anna Artati, et al.min, 20,000 x g, 4°C). The supernatant was transferred to a fresh tube and proteins were removed using Deproteinizing Sample Preparation Kit – TCA (Abcam) according to the manufacturer’s instructions. For the measurement of glutathione (GSH) levels

(8) Cancer cell-intrinsic inflammasome protein ASC links innate immunity with mitochondrial metabolism in driving pancreatic cancerNature CommunicationsFebruary 7, 2026Yu C. J. Chey, Bassam Kashgari, Louise McLeod, Georgette A. Radford, Linden J. Gearing, Ruby E. Dawson, Malvika Kharbanda, Joanne Lundy, Daniel Croagh, Charlotte Girard-Guyonvarc’h, et al.Dounce homogenizer and deproteinized using the Deproteinizing Sample Preparation Kit – TCA (Abcam, ab204708) prior to the assay. Readings were normalized to cell protein concentrations measured by Pierce BCA

(9) Assessment of the angiogenic potential of xenografted tumors by biomedical imaging techniquesBiomed Opt ExpressFebruary 5, 2026Irina Druzhkova, Anna Orlova, Pavel Subochev, Alexey Kurnikov, Anna Glyavina, Alina Isakova, Ekaterina Kukovyakina, Ekaterina Plotnikova, Marine Gasparian, Anastasia Komarova, et al.24 h. Then the culture medium was collected and proteins were removed using a deproteinization kit (ab204708, Abcam, USA). Samples were analyzed using a colorimetric lactate assay kit (ab65331, Abcam, USA) and

(10) Lipidomic Analysis of Plasma Extracellular Vesicles from Adiponectin Deficient Mice or Metabolic Syndrome Patients Reveals Pro-Oxidative and Pro-Inflammatory Lipid Signatures Correlating with Metabolic DysfunctionJournal of extracellular vesiclesFebruary 1, 2026Cho S, Sung HK, Nguyen K, Lei Y, Wannaiampikul S, Lee B, Tam E, Luo Y, Petrotchenko EV, Borchers CH, et al.the reaction, the samples were deproteinized using the Deproteinizing Sample Preparation Kit (Abcam, ab204708). The optical density was measured at 570 nm using a plate reader (Thermo Fisher, VLBLATGD0). 2.15

(11) Cedrol ameliorates inflammatory bowel disease via mitochondrial biogenesis, gut microbiota restoration, and intestinal barrier repairFrontiers in PharmacologyJanuary 2, 2026Mo-Rong Xu, Chia-Hsin Lin, Chung-Hsuan Wang, Sheng-Yang WangTNF-α Valuvine™ ELISA (VAL609, Novus Biologicals, China), Deproteinizing Sample Preparation Kit - TCA (ab204708, Abcam, UK), ATP Assay Kit (Colorimetric/Fluorometric) (ab83355, Abcam, UK), CheKine™ Micro Lipid Peroxidation

(12) Spatial heterogeneity of lactylation: insights into gene expression, metabolism, and lactate transport in human embryonic stem cellsBiology OpenJanuary 1, 2026Alexandra M. Kozlov, Zuleika C. L. Leung, Rachel B. Wilson, Sukhdeep Bhangal, Karen Nygard, Andrew M. Powell, Nica M. Borradaile, Dean H. Betts, Robert C. Cummingcentrifuged at 12,000 g for 5 min at 4°C. The supernatant was isolated and treated with 10 μl cold 30% KOH (#ab204708; Abcam), vortexed for 5 s, vented, and incubated on ice for 5 min before storage at −80°C. Cells were

(13) Helicobacter pylori infection aggravates hepatic steatosis by lactylation-driven WTAP-mediated m6A modificationGut MicrobesDecember 31, 2025Han Chen, Zi Wang, Yan Wang, Shuo Li, Wei Su, Yuting Shao, Guoxin Zhang, Yun Liu, Qiang Ye, Xiaoying Zhoulysed with lysis buffer. Cell lysates were deproteinized using a TCA-based preparation kit (Abcam, ab204708) to eliminate endogenous LDH activity, and lactate levels were determined using the L-Lactate Assay

(14) IGF2BP3 binds to FBXO32 to activate the cyclic guanosine monophosphate-protein kinase G pathway, promoting gastric cancer progressionWorld J GastroenterolDecember 14, 2025Yi Si, Bo Tian, Rui Zhang, Ming-Da Xuan, Kun-Yi Liu, Jiao Jiao, Shuang-Shuang Han, Hong-Fei Li, Yan-Hong Hu, Hong-Yan Zhao, et al.cell lysate was deproteinized using deproteinizing sample preparation kit tricarboxylic acid (Abcam, ab204708). The deproteinized cell lysates were then mixed with the reaction mixtures, followed by incubation

(15) Lactate‐mediated activation of GPR81 regulates BCR /Abl protein expression in chronic myeloid leukemia cells selected under low oxygen tensionThe Journal of PathologyDecember 1, 2025Giulio Menegazzi, Dayana Desideri, Alessio Biagioni, Elisabetta Rovida, Persio Dello Sbarba, Silvia PeppicelliFinally, enzymes were removed from the supernatant using the Deproteinizing Sample Preparation Kit (ab204708, Abcam, Prodotti Gianni, Milan, Italy), following the manufacturer's protocol. L‐lactate concentrations

(16) A niche-dependent redox rheostat regulates epithelial stem cell fateNature CommunicationsNovember 28, 2025Xi Chen, Krishnan Raghunathan, Bin Bao, Elsy Ngwa, Andrew Kwong, Zhongyang Wu, Stephen Babcock, Clara Baek, George Ye, Anoohya Muppirala, et al.uniformed to 500 μg/ml by BCA analysis (Thermo Scientific™, 23227). After deproteinization (Abcam, ab204708), we then ran GSH and Total Glutathione assay following the manufacturer’s instructions (Abcam, ab205811). Gel electrophoresis

(17) Dietary Polyunsaturated Fatty Acids Regulate Dendritic Cell Function via Nrf2-dependent Control of FerroptosisResearch SquareNovember 19, 2025Deepika Cubillos-Ruiz, Deepika Awasthi, Camilla Salvagno, Sung-Min Salvagno, Sung-Min Sandoval, Chang-Suk Sandoval, Wiam A. M. Madani, Jacqueline Madani, Jacqueline Crater, Kaushik Sen, et al.NP-40, and lysates were deproteinized using the Deproteinizing Sample Preparation Kit – TCA (Abcam, Cat# ab204708). Deproteinized lysates were used to quantify total glutathione levels using the Glutathione Assay Kit

(18) Neuronal glycogen breakdown mitigates tauopathy via pentose-phosphate-pathway-mediated oxidative stress reductionNature MetabolismJune 30, 2025Sudipta Bar, Kenneth A. Wilson, Tyler A. U. Hilsabeck, Sydney Alderfer, Eric B. Dammer, Jordan B. Burton, Samah Shah, Anja Holtz, Enrique M. Carrera, Jennifer N. Beck, et al.tissue lysate was used for the assay. Deproteinization was performed using a kit from Abcam (cat. no. ab204708). PKA activity was measured using a kit protocol (Thermo Fisher cat. no. EIAPKA). For PKA activity,

(19) The RAGE Inhibitor TTP488 (Azeliragon) Improves Diabetic Bladder Dysfunction in Leptin-Deficient Obese MiceAntioxidants (Basel)June 27, 2025Akila Lara Oliveira, Matheus Leite Medeiros, Antonio Thiago Pereira Campos, Carlos Lenz Cesar, Fabiola Zakia Mónica, Edson Antunespreviously deproteinized using a Deproteinizing Sample Preparation Kit (TCA, Abcam, Cambridge, UK, ab204708), according to the manufacturer’s instructions. Levels of MG-H1 were measured in deproteinized samples

(20) PCSK9 deficiency promotes the development of peripheral neuropathyJCI InsightJune 23, 2025Ali K. Jaafar, Aurélie Paulo-Ramos, Guillaume Rastoldo, Bryan Veeren, Cynthia Planesse, Matthieu Bringart, Philippe Rondeau, Kévin Chemello, Olivier Meilhac, Gilles C. Lambert, et al.signals at 570 nm, samples were deproteinized with a Deproteinizing Sample Preparation Kit (Abcam, ab204708). Protein extracts were obtained after lysis of tissues in 350-μL Tris HCl buffer (pH 9.5; 10 mM) containing

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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