iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions 1725035 from Bio-Rad

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ItemiScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions
CompanyBio-Rad
Price $671.00Supplier Page View Company Product Page
Catalog Number1725035

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) A high-throughput screen for nucleolar function reveals a role for the signaling protein, SPRR3, in ribosome biogenesisJournal of Biological ChemistryMarch 1, 2026Emily C. Sutton, Carson J. Bryant, Janina I.S. Gbenoba, Isabella R. Lawrence, Susan J. BasergacDNA was synthesized from 1 μg total input RNA using iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). In a qPCR plate (Bio-Rad MLL9601), 1 μl of cDNA was mixed with 19 μl of a qPCR master mix containing

(2) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.Plus Micro kit (74034, Qiagen). cDNA was synthesized using the iScript gDNA Clear cDNA Synthesis kit (1725035, Bio-Rad, USA) and a Bio-Rad C1000 Touch ThermoCycler (Bio-Rad). RNA expression was assessed in the

(3) Decreased endothelial cell retinoic acid signaling accelerates progression of single ventricle pulmonary arteriovenous malformationsbioRxivDecember 21, 2025Henry Rousseau, Tina Wan, Nhi Nguyen, Jaime Wendt Wendt Andrae, Michael Tschannen, Angela J. Mathison, Victor Jin, Olivia Groh, Xingyan Zhou, Stryder M. Meadows, et al.qPCR. Total RNA (250 ng) was transcribed into cDNA (iScript gDNA clear cDNA synthesis kit; BioRad, #1725035), and qPCR was performed (PR1MA qMax green qPCR mix; MidSci, #PR2000-N-1000) on the CFX96 qPCR machine

(4) Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory PropertiesAdvanced ScienceDecember 9, 2025Amy J. Hulme, Rocio K. Finol‐Urdaneta, Jeffrey R. McArthur, Nicholas R. Marzano, Simon Maksour, Amarinder Thind, Yang Guo, Dominic Kaul, Marnie Maddock, Oliver Friedrich, et al.removed, and the RNA was reverse transcribed into cDNA using the iScript gDNA Clear cDNA Synthesis Kit (1725035, Bio‐Rad), as per the manufacturer's guidelines. RT‐qPCR was conducted using the PowerUP SYBR green master mix

(5) A spatial atlas of the complement system uncovers unique expression patterns in postnatal brain development in miceNat CommunDecember 2, 2025Yingying Zhang, Brianna Watson, Ajitanuj Rattan, Tyrone Lee, Smriti Chawla, Ludwig Geistlinger, Yilin Guan, Finley B. Lord, Minghe Ma, Takashi Miwa, et al.Genomic DNA removal and cDNA synthesis were performed with iScript gDNA clear cDNA synthesis kit (Bio-Rad 1725035) by following manufacturer’s instructions. ddPCR reactions were run with QX200™ ddPCR™ EvaGreen Supermix

(6) Dynamic control of autoreactive B cells from spontaneous germinal centersThe Journal of ImmunologyDecember 1, 2025Kristine Oleinika, Alexia Correia Correia Ferreira, Selma Mouftakir, Carlos Castrillon, Lisa Madungwe, Usha Nair, Facundo D. Batista, Michael C. Carroll(25°C for 5min, 46°C for 20min, 95°C for 1min) using the iScript gDNA clear cDNA synthesis kit (Bio-Rad,1725035). PCR was performed using GoTaq Flexi reagents (Promega, M8298) with 20ng template DNA, 0.2 mM dNTPs

(7) Protocol for triggering plant gene silencing by viral delivery of short RNASTAR ProtocolsDecember 1, 2025Arcadio García, Verónica Aragonés, Paloma Ortiz-García, José-Antonio Daròs, Fabio Pasinpeptides, and recombinant proteins" ,, iScript gDNA clear cDNA synthesis kit,Bio-Rad Laboratories,1725035 SsoAdvanced universal SYBR green supermix,Bio-Rad Laboratories,1725271 Tryptone,Condalab,1612 Yeast

(8) DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separationJournal of Biological ChemistryDecember 1, 2025Adenine Si-Hui Koo, Weiyan Jia, Sang Hwa Kim, Mark Scalf, Claire E. Boos, Yuhong Chen, Demin Wang, Andrew F. Voter, Aditya Bajaj, Lloyd M. Smith, et al.by iScript cDNA Synthesis Kit (Bio-Rad, 1708891) or iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) according to manufacturer’s protocols. Human RIF1-Ex32 splicing was evaluated by RT-PCR using primers

(9) Autoreactive immunoglobulin G levels and Fc receptor γ subunit upregulation drive mechanical allodynia after nerve constriction or crush injuryPainDecember 1, 2025Nathan T. Fiore, Kendal F. Willcox, Anamaria R. Grieco, Dorsa Dayani, Younus A. Zuberi, Cobi J. Heijnen, Peter M. GraceQiagen, Hilden Germany) and cDNA was generated by reverse transcription using iScript gDNA Clear kits (1725035 Bio-Rad, CA USA) following manufacturer instructions. RT-qPCR was performed by diluting cDNA and associated

(10) UBE3A stabilization of β-catenin preserves synaptic proteins essential for motor and cognitive functions in Angelman SyndromeMolecular AutismNovember 27, 2025Xin Yang, Yuwen-Alvin Huang, John MarshallRNeasy Mini Kit (74104). cDNA was synthesized by the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035) after potential DNA contamination was cleaned. Quantitative real-time PCR was performed using the CFX96 Touch Deep Well Real-Time PCR Detection System

(11) Evaluation of the Activity of Monensin and Its Analogs for Modulation of Stem-like Cell Functionality in 2D and 3D Breast Cancer ModelsACS Pharmacology & Translational ScienceNovember 14, 2025Alicja Urbaniak, Billie Heflin, Eric R. Siegel, Drew Seale, Megan R. Reed, James S. Nix, Eric U. Yee, Marta Jędrzejczyk, Greta Klejborowska, Natalia Stępczyńska, et al.and reverse transcription were performed using the iScript gDNA Clear cDNA Synthesis Kit (cat. no. 1725035, Bio-Rad, Hercules, CA, USA). The levels of specific transcripts were quantified by qPCR using the following

(12) WNT signalling promotes NF-κB activation and drug resistance in KRAS-mutant colorectal cancerEMBO ReportsNovember 4, 2025Bojie Cong, Evangelia Stamou, Kathryn Pennel, Teena Thakur, Molly Mckenzie, Amna Matly, Kathryn Gilroy, Harshit Shah, Sindhura Gopinath, Joanne Edwards, et al.Life Technologies). mRNA was reverse transcribed using iScriptTM gDNA Clear cDNA Synthesis Kit (cat# 1725035, Bio-Rad Laboratories Ltd). For quantitative Real-Time PCR (qPCR), iTaq™ Universal SYBR® Green Supermix

(13) Deficiency in the msbB Gene Reduced the Salmonella Typhimurium Virulence Through Mechanisms Beyond LPS ModificationMicroorganismsOctober 31, 2025Ling Yang, Zhuodong Chai, Jiaqian Qi, Yan Zhang, Yuqi Zhou, Zhenyu Li, Yinan WeiUSA). 500 ng of RNA were reverse transcribed using an iScript™ gDNA Clear cDNA Synthesis Kit (cat# 1725035, Bio-Rad, Hercules, CA, USA). Real-time PCR was performed using iTaq SYBR green supermix (Bio-Rad, 1725121)

(14) Damage-induced IL-18 stimulates thymic NK cells limiting endogenous tissue regenerationNature immunologyOctober 1, 2025Granadier D, Cooper K, Acenas D 2nd, Kousa A, Warren M, Hernandez V, Iovino L, deRoos P, Lederer EE, Shannon-Sevillano S, et al.Plus Micro kit (74034, Qiagen). cDNA was synthesized using the iScript gDNA Clear cDNA Synthesis kit (1725035, Bio-Rad) and a Bio-Rad C1000 Touch ThermoCycler (Bio-Rad). RNA expression was assessed using the Bio-Rad

(15) Bioelectric characterization of senescing human keratinocytesiScienceSeptember 19, 2025Hamid Sediqi, Michael Levin(Cat# 74104). 200 ng of RNA was reverse transcribed using iScript™ cDNA Synthesis Kit (Bio-Rad, Cat# 1725035). qPCR was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher, Cat# A25777). The following

(16) Targeting carbonic anhydrase IX/XII prevents the anti‐ferroptotic effect of stromal lactic acid in prostate carcinomaMolecular OncologySeptember 1, 2025Elisa Pardella, Giuseppina Comito, Luigi Ippolito, Erica Pranzini, Marta Iozzo, Giulia Gangarossa, Francesca Virgilio, Silvia Bua, Alessio Nocentini, Giada Sandrini, et al.synthesized from 500 to 1000 ng of total extracted RNA using the iScript cDNA Synthesis Kit (BioRad, #1725035) and the T100 ThermalCycler (BioRad), according to the manufacturer's instructions. qRT‐PCR was performed

(17) Ume6 protein complexes connect morphogenesis, adherence and hypoxic genes to shape Candida albicans biofilm architectureNature MicrobiologyAugust 21, 2025Eunsoo Do, C. Joel McManus, Robert Zarnowski, Manning Y. Huang, Katharina Goerlich, David R. Andes, Aaron P. MitchellRNA per sample was used for cDNA synthesis using an iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) with diluted cDNA, following the manufacturer’s instruction. Then, qRT-PCR was conducted using the

(18) Molecular and functional MRI enables detection of cardiac fibrosis and evaluation of treatment response after chordin-like 1 gene therapy in myocardial infarctionTheranosticsAugust 8, 2025Konstantina Amoiradaki, Mateusz Tomczyk, Xiaoying Wang, Gastão Cruz, Carlos Velasco, Lorena Zentilin, Francesca Bortolotti, Claudia Prieto, René M. Botnar, Mauro Giacca, et al.with DNase I (Roche) and then reverse transcribed using the iScriptTM gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad, USA) following the manufacturer's protocol. Quantification of gene expression was performed

(19) Crosstalk between the aryl hydrocarbon receptor and hypoxia-inducible factor 1α pathways in human islet modelsIsletsJuly 18, 2025Noa Gang, Kyle A. van Allen, William G. Willmore, Francis C. Lynn, Jennifer E. Bruintreatment was performed prior to cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). Real time (RT) qPCR was performed using SsoAdvanced Universal SYBR Green Supermix

(20) Selection of stable reference genes for accurate reverse-transcription quantitative PCR in cotton-herbivore studies using virus-induced gene silencingSci RepJuly 8, 2025Mason K. Clark, Spencer T. Behmer, Gregory A. Swordthe reaction. cDNA synthesis was performed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) following the manufacturer’s instructions with 500 ng input RNA in 20µL reactions. Optimal cDNA input

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iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions

iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions from Bio-Rad

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iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions from Bio-Rad

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