iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions 1725035 from Bio-Rad

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ItemiScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions
CompanyBio-Rad
Price $671.00Supplier Page View Company Product Page
Catalog Number1725035

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

(1) Synthetic DNA vaccine platform elicits potent immunity where electroporated naked-mRNA is non-immunogenicJ Immunother CancerMay 13, 2026Claudia Tonini, Andrea Bianchi, Mauro Esposito, Ilaria Esposito, Tiziano Giacomelli, Emily Young, Heikki Lanckriet, Zoe Whiffen, Milena Pavlickova, Deborah Entiriwaa, et al.The complementary DNA (cDNA) was obtained by using iScript gDNA clear cDNA Synthesis Kit (Bio-Rad; 1725035). The target region, as for β-actin housekeeping gene, used for loading control was amplified with iTaq

(2) Enforced ZFP281 expression delays breast cancer initiation and can provide lifelong protection against breast cancer metastasisbioRxivApril 18, 2026Deepak K. Singh, Hongwei Zhou, Nyima Sherpa, Xiang Yu Zheng, Artem Lomakin, Pedram Razghandi, Xin Hunag, Rama Kadamb, Suryansh Shukla, Luis E. Valencia Salazar, et al.74034). Reverse transcription was performed using the iScript gDNA clear cDNA synthesis kit (BioRad, 1725035). Relative expression levels of RNA were determined using PowerUP SYBR™ Green master Mix (Applied Biosystems,

(3) TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regenerationCommunications BiologyApril 2, 2026Neena Lala-Tabbert, Allan Humphrey, Diana Ratsun, Nathalie Earl, Martine St-Jean, Eric C. LaCasse, Robert G. Kornelukmanufacturer’s protocol. Complementary DNA (cDNA) was generated using iScript gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad Laboratories) following manufacturer’s protocol. RT-qPCRs were performed using SsoAdvanced Universal SYBR® Green Supermix

(4) FADD is recruited to activated STING oligomers to initiate caspase-mediated NF-κB activation in Drosophila melanogasterThe EMBO JournalMarch 28, 2026Kasper Grønbjerg Winther, Juliette Schneider, Gabrielle Haas, Anna Hvarregaard Christensen, Shreya Goyal, Weisheng Luo, Ziming Wei, Jiyong Liu, Katarzyna Kjøge, Jan J Enghild, et al.2022",10.3389/fimmu.2022.933137 TRIzol,Invitrogen,15596078 iScript gDNA Clear cDNA Synthesis Kit,Bio-Rad,1725035 SYBR Green master mix,Accurate Biotechnology (Hunan),Code. AG11701 Drosophila C virus (DCV),"Sabatier

(5) A high-throughput screen for nucleolar function reveals a role for the signaling protein, SPRR3, in ribosome biogenesisJournal of Biological ChemistryMarch 1, 2026Emily C. Sutton, Carson J. Bryant, Janina I.S. Gbenoba, Isabella R. Lawrence, Susan J. BasergacDNA was synthesized from 1 μg total input RNA using iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). In a qPCR plate (Bio-Rad MLL9601), 1 μl of cDNA was mixed with 19 μl of a qPCR master mix containing

(6) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.Plus Micro kit (74034, Qiagen). cDNA was synthesized using the iScript gDNA Clear cDNA Synthesis kit (1725035, Bio-Rad, USA) and a Bio-Rad C1000 Touch ThermoCycler (Bio-Rad). RNA expression was assessed in the

(7) Decreased endothelial cell retinoic acid signaling accelerates progression of single ventricle pulmonary arteriovenous malformationsbioRxivDecember 21, 2025Henry Rousseau, Tina Wan, Nhi Nguyen, Jaime Wendt Wendt Andrae, Michael Tschannen, Angela J. Mathison, Victor Jin, Olivia Groh, Xingyan Zhou, Stryder M. Meadows, et al.qPCR. Total RNA (250 ng) was transcribed into cDNA (iScript gDNA clear cDNA synthesis kit; BioRad, #1725035), and qPCR was performed (PR1MA qMax green qPCR mix; MidSci, #PR2000-N-1000) on the CFX96 qPCR machine

(8) Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory PropertiesAdvanced ScienceDecember 9, 2025Amy J. Hulme, Rocio K. Finol‐Urdaneta, Jeffrey R. McArthur, Nicholas R. Marzano, Simon Maksour, Amarinder Thind, Yang Guo, Dominic Kaul, Marnie Maddock, Oliver Friedrich, et al.removed, and the RNA was reverse transcribed into cDNA using the iScript gDNA Clear cDNA Synthesis Kit (1725035, Bio‐Rad), as per the manufacturer's guidelines. RT‐qPCR was conducted using the PowerUP SYBR green master mix

(9) A spatial atlas of the complement system uncovers unique expression patterns in postnatal brain development in miceNat CommunDecember 2, 2025Yingying Zhang, Brianna Watson, Ajitanuj Rattan, Tyrone Lee, Smriti Chawla, Ludwig Geistlinger, Yilin Guan, Finley B. Lord, Minghe Ma, Takashi Miwa, et al.Genomic DNA removal and cDNA synthesis were performed with iScript gDNA clear cDNA synthesis kit (Bio-Rad 1725035) by following manufacturer’s instructions. ddPCR reactions were run with QX200™ ddPCR™ EvaGreen Supermix

(10) DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separationJournal of Biological ChemistryDecember 1, 2025Adenine Si-Hui Koo, Weiyan Jia, Sang Hwa Kim, Mark Scalf, Claire E. Boos, Yuhong Chen, Demin Wang, Andrew F. Voter, Aditya Bajaj, Lloyd M. Smith, et al.by iScript cDNA Synthesis Kit (Bio-Rad, 1708891) or iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) according to manufacturer’s protocols. Human RIF1-Ex32 splicing was evaluated by RT-PCR using primers

(11) Protocol for triggering plant gene silencing by viral delivery of short RNASTAR ProtocolsDecember 1, 2025Arcadio García, Verónica Aragonés, Paloma Ortiz-García, José-Antonio Daròs, Fabio Pasinpeptides, and recombinant proteins" ,, iScript gDNA clear cDNA synthesis kit,Bio-Rad Laboratories,1725035 SsoAdvanced universal SYBR green supermix,Bio-Rad Laboratories,1725271 Tryptone,Condalab,1612 Yeast

(12) Autoreactive immunoglobulin G levels and Fc receptor γ subunit upregulation drive mechanical allodynia after nerve constriction or crush injuryPainDecember 1, 2025Nathan T. Fiore, Kendal F. Willcox, Anamaria R. Grieco, Dorsa Dayani, Younus A. Zuberi, Cobi J. Heijnen, Peter M. GraceQiagen, Hilden Germany) and cDNA was generated by reverse transcription using iScript gDNA Clear kits (1725035 Bio-Rad, CA USA) following manufacturer instructions. RT-qPCR was performed by diluting cDNA and associated

(13) Dynamic control of autoreactive B cells from spontaneous germinal centersThe Journal of ImmunologyDecember 1, 2025Kristine Oleinika, Alexia Correia Correia Ferreira, Selma Mouftakir, Carlos Castrillon, Lisa Madungwe, Usha Nair, Facundo D. Batista, Michael C. Carroll(25°C for 5min, 46°C for 20min, 95°C for 1min) using the iScript gDNA clear cDNA synthesis kit (Bio-Rad,1725035). PCR was performed using GoTaq Flexi reagents (Promega, M8298) with 20ng template DNA, 0.2 mM dNTPs

(14) UBE3A stabilization of β-catenin preserves synaptic proteins essential for motor and cognitive functions in Angelman SyndromeMolecular AutismNovember 27, 2025Xin Yang, Yuwen-Alvin Huang, John MarshallRNeasy Mini Kit (74104). cDNA was synthesized by the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035) after potential DNA contamination was cleaned. Quantitative real-time PCR was performed using the CFX96 Touch Deep Well Real-Time PCR Detection System

(15) Evaluation of the Activity of Monensin and Its Analogs for Modulation of Stem-like Cell Functionality in 2D and 3D Breast Cancer ModelsACS Pharmacology & Translational ScienceNovember 14, 2025Alicja Urbaniak, Billie Heflin, Eric R. Siegel, Drew Seale, Megan R. Reed, James S. Nix, Eric U. Yee, Marta Jędrzejczyk, Greta Klejborowska, Natalia Stępczyńska, et al.and reverse transcription were performed using the iScript gDNA Clear cDNA Synthesis Kit (cat. no. 1725035, Bio-Rad, Hercules, CA, USA). The levels of specific transcripts were quantified by qPCR using the following

(16) WNT signalling promotes NF-κB activation and drug resistance in KRAS-mutant colorectal cancerEMBO ReportsNovember 4, 2025Bojie Cong, Evangelia Stamou, Kathryn Pennel, Teena Thakur, Molly Mckenzie, Amna Matly, Kathryn Gilroy, Harshit Shah, Sindhura Gopinath, Joanne Edwards, et al.Life Technologies). mRNA was reverse transcribed using iScriptTM gDNA Clear cDNA Synthesis Kit (cat# 1725035, Bio-Rad Laboratories Ltd). For quantitative Real-Time PCR (qPCR), iTaq™ Universal SYBR® Green Supermix

(17) ECD co-operates with ERBB2 to promote tumorigenesis through upregulation of unfolded protein response and glycolysisCancer LettersNovember 1, 2025Benjamin B. Kennedy, Mohsin Raza, Sameer Mirza, Asher Rajkumar Rajan, Farshid Oruji, Matthew M. Storck, Subodh M. Lele, Timothy E. Reznicek, Lusheng Li, M. Jordan Rowley, et al.The RNA was reverse transcribed into cDNA using the BioRad iScript gDNA Clear cDNA Synthesis Kit (cat#1725035, Bio-Rad). For real-time (RT)-qPCR, triplicate samples were analyzed using Applied Biosystems SYBR Green

(18) Deficiency in the msbB Gene Reduced the Salmonella Typhimurium Virulence Through Mechanisms Beyond LPS ModificationMicroorganismsOctober 31, 2025Ling Yang, Zhuodong Chai, Jiaqian Qi, Yan Zhang, Yuqi Zhou, Zhenyu Li, Yinan WeiUSA). 500 ng of RNA were reverse transcribed using an iScript™ gDNA Clear cDNA Synthesis Kit (cat# 1725035, Bio-Rad, Hercules, CA, USA). Real-time PCR was performed using iTaq SYBR green supermix (Bio-Rad, 1725121)

(19) Damage-induced IL-18 stimulates thymic NK cells limiting endogenous tissue regenerationNature immunologyOctober 1, 2025Granadier D, Cooper K, Acenas D 2nd, Kousa A, Warren M, Hernandez V, Iovino L, deRoos P, Lederer EE, Shannon-Sevillano S, et al.Plus Micro kit (74034, Qiagen). cDNA was synthesized using the iScript gDNA Clear cDNA Synthesis kit (1725035, Bio-Rad) and a Bio-Rad C1000 Touch ThermoCycler (Bio-Rad). RNA expression was assessed using the Bio-Rad

(20) Bioelectric characterization of senescing human keratinocytesiScienceSeptember 19, 2025Hamid Sediqi, Michael Levin(Cat# 74104). 200 ng of RNA was reverse transcribed using iScript™ cDNA Synthesis Kit (Bio-Rad, Cat# 1725035). qPCR was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher, Cat# A25777). The following

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iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions

iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions from Bio-Rad

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iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions from Bio-Rad

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