Description
Enables isolation of the highest levels of transfection-grade plasmid DNA from a single
E.coli mega prep using an enhanced anion exchange resin.
The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Megaprep Kit typically isolates 4mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 500mL of bacterial culture.
Advantages of the PureLink™ HiPure Expi Plasmid Megaprep Purification Kit include:
- High yield—isolate over 5mg of high quality plasmid DNA from a single purification using 1L of bacterial culture volume
- Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for mammalian cell transfection
- Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation takes as little as 90 minutes
HiPure Expi anion exchange chromatography for plasmid purificationPureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.
For Research Use Only. Not for use in diagnostic procedures.