Granzyme B ELISA DY2906-05 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGranzyme B ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $450.00Supplier Page View Company Product Page
Catalog NumberDY2906-05
Quantity1 Kit (for 5 Plates)
ApplicationsELISA
Detection Range39.10 - 2500 pg/mL
ReactivityHuman
Detection TargetGranzyme B
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namegranzyme B
NCBI Gene AliasesC11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Specifications/Features

Available conjugates: Sizes Available: 1 Kit (for 5 Plates)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Whole-protein screening and multi-modal profiling of antigen-specific CD4+ T cells at single-cell resolutionNat CommunMay 12, 2026Rongyu Zhang, Jingqi Qi, Michaela McKasson, Jongchan Choi, Vanessa Gutierrez, Conor Brennan, Sunga Hong, William Chour, Rachel H. Ng, Jingyi Xie, et al.were performed according to the manufacturer’s manual (R&D Systems, DY202-05, DY285B-05, DY210-05, and DY2906-05). Cytotoxicity assay The cytotoxic assay was conducted through IncuCyte. DR1-K562 cells were preloaded

(2) Single-cell transcriptomic analysis reveals intra-tumoral heterogeneity and immunotherapy strategies in high-grade serous ovarian canceriScienceApril 17, 2026Chunhui Gao, Xiaogang Lv, Anqi Pan, Lipai Chen, Qianqian Liu, Donghui Zhang, Wenjuan WuCD133+ IFIT1- cells were analyzed to measure IFN-γ or GZMB levels using ELISA kits (R&D Systems, QK285, DY2906-05). The assays were performed in triplicates according to the manufacturer’s instructions. Quantification

(3) Single nuclei/cell transcriptomics reveal DMD driven cell dynamics and mechanisms of fibroblast inflammatory tissue priming in human dystrophic muscleResearch SquareApril 15, 2026M. Carrie Chesmore, Florian Barthélémy, Deirdre Scripture-Adams, Deirdre D. Scripture-Adams, Annabel Little, Richard Nasif, Shirley Nieves-Rodriguez, Richard T. Corona, Shirley Nieves-Rodriguez, Rosario I. Corona, et al.T cells Supernatants of expanded T cells were used to measure the secretion of GZMK (Abcam, ab31436), GZMB (R&D system DY290605), TNFα (R&D system DTA00D), and IFNγ (Thermofisher ENEHIFNG5) by ELISA. Antibody labeling for feature

(4) PCSK9-mediated degradation of cell-surface LDL receptors impairs human CD8+ T cell effector functionsiScienceMarch 20, 2026Angela Markovska, Lara F. Lommers, Alejandra Bodelón, Patrick Greve, Leonie C. van Vark-van der Zee, Monique T. Mulder, Jeanine E. Roeters van Lennep, Noam Zelcer, Henk S. Schipper, Marianne Boeskit,Miltenyi,130-096-495 T cell activator CD3/CD28 Dynabeads,Gibco,11131D granzyme B ELISA kit,R&D systems,DY2906-05 IFNγ ELISA kit,Biolegend,430104 RNeasy mini kit,Qiagen,74104 cDNA Synthesis Kit,Bio-rad,1708890 eBioscience™

(5) A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based constructMolecular Therapy OncologyMarch 19, 2026Alexandros Kourtesakis, Hiu Nam Hannah Chow, Eileen Bailey, Sandra Horschitz, Ammar Jabali, Rainer Will, Christoph Schifflers, Abigail K. Suwala, Hannah Rohdjess, Melissa Hahn, et al.quantify cytokine secretion: Human Granzyme-B DuoSet enzyme-linked immunosorbent assay kit (ELISA; #DY2906–05, R&D Systems), Human IFN-gamma DuoSet ELISA kit (#DY285B, R&D Systems), Human TNF-alpha DuoSet ELISA

(6) Overactivation of Cdc42 GTPase Impairs the Cytotoxic Function of NK Cells From Old Individuals Towards Senescent FibroblastsAging CellFebruary 8, 2026Albert Kallon Koroma, Karmveer Singh, Yongfang Wang, Linda Krug, Philipp Haas, Meinhard Wlaschek, Vadim Sakk, Tanja Schuster, Daniel Fürst, Hubert Schrezenmeier, et al.,Stem Cell,Cat. #19855 FCCP,Sigma‐Aldrich,Cat. #C2920 Human granzyme B DouSet Elisa Kit,R&D Systems,Cat #DY2906‐05 LumiGlo/Peroxide,Cell Signaling,Cat. #7003 Magnesium chloride (MW95.21),Sigma‐Aldrich,Cat. #7786‐30‐3 NEBNext

(7) Synthesis of Bispecific Conjugates by ADP‐Ribosyl CyclasesAdvanced ScienceDecember 21, 2025Sunny H. Kim, Arshad J. Ansari, Lei Zhang, Guoyun Kao, Thuc Oanh Hoang, Zeyu Zhang, Srinivasarao Singireddi, Benjamin B. Katz, Yong Zhangfrom New England Biolabs, MA. Human IFN‐gamma DuoSet ELISA (DY285B) and human granzyme B DuoSet ELISA (DY2906‐05) were purchased from R&D Systems, MN. ZymoPURE maxiprep kits (11‐555) were purchased from ZymoResearch,

(8) REACtiVe-2: phase I evaluation of dendritic cell vaccination and agonistic CD40 therapy following (m)FOLFIRINOX in metastatic pancreatic cancerNature CommunicationsNovember 28, 2025Songul Kucukcelebi, Freek R. van ‘t Land, Sjoerd H. van der Burg, Ferry A. L. M. Eskens, Marjolein Y. V. Homs, Marcella Willemsen, Anne Onrust-van Schoonhoven, Nina E. M. Rozendaal, Amine Fellah, Disha Vadgama, et al.Supplementary Table 8 for a list of antibodies used). IFN-γ (#DY285B-05, R&D Systems) and Granzyme B (#DY2906-05, R&D Systems) levels in the supernatant were detected using ELISA. Immunohistochemistry Formalin-fixed, paraffin-embedded

(9) Suppression of tumor growth by GMI, an edible fungal immunomodulatory protein, is associated with targeting GSK3β‑mediated proteasomal degradation of PD‑L1Int J Mol MedOctober 7, 2025Wei-Jyun Hua, Li-Chen Huang, Zhi-Hu Lin, Yi-Ru Ciou, Kai-Fan Lin, Li-Lan Liao, Wei-Hung Hsu, Tung-Yi LinHuman IL-2 and Granzyme B ELISA Kit (BioLegend, Inc.; cat. no. 431804; and R&D Systems, Inc.; cat. no. DY2906-05, respectively). The assay was conducted according to the manufacturer's protocols, as previously described

(10) LncRNA-CTD suppresses metastasis and immune evasion by modulating snail1 and MHC-I expression in colorectal cancerJournal for immunotherapy of cancerSeptember 15, 2025Xu N, Qiu H, Sun Y, Jin H, Yan S, Zhang F, Song Y, Zheng G, Liao D, Wei X, et al.IFN-γ, TNF-α (Tumor Necrosis Factor-alpha) or GZMB levels using ELISA kits (R&D Systems, QK285, DTA00D, DY2906-05). The assays were performed in triplicates according to the manufacturer’s instructions. Tumor models Animal experiments

(11) Secretion of a VEGF-Blocking scFv Enhances CAR T-cell PotencyCancer Immunology ResearchAugust 1, 2025Valentina M. Supper, Hannah Donner, Filippo Birocchi, Alexandra Bratt, Giulia Escobar, Michael C. Kann, Sangwoo Park, Grace Martin, Felix Korell, Hana Takei, et al.Supernatant was collected from CAR T cells on day 13 of production. ELISA’s for Granzyme B (R&D Systems DY2906–05), TNF-α (R&D Systems DY210–05), IFN-γ (R&D Systems DY285B), and IL2 (R&D Systems, DY202) were performed

(12) Secretion of a VEGF-blocking scFv enhances CAR T-cell potencyCancer Immunol ResAugust 1, 2025Valentina M. Supper, Hannah Donner, Filippo Birocchi, Alexandra Bratt, Giulia Escobar, Michael C. Kann, Sangwoo Park, Grace Martin, Felix Korell, Hana Takei, et al.Supernatant was collected from CAR T cells on day 13 of production. ELISA’s for Granzyme B (R&D Systems DY2906–05), TNF-α (R&D Systems DY210–05), IFN-γ (R&D Systems DY285B), and IL2 (R&D Systems, DY202) were performed

(13) Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemiaNature Biomedical EngineeringJuly 1, 2025Chao Ma, Huishu Wang, Lunan Liu, Ruiqi Chen, Nandana Mukherjee, Jie Tong, Shadab Kazmi, Xiangyi Fang, Matthew T. Witkowski, Iannis Aifantis, et al.Human Perforin ELISA Set (catalogue number ab83709, Abcam), Human Granzyme B Set (catalogue number DY2906-05, R&D Systems), Human CCL1/I-309 DuoSet (catalogue number DY272, R&D Systems) and MIP-1β Human Instant

(14) Utilization of universal-targeting mSA2 CAR-T cells for the treatment of glioblastomaOncoimmunologyJune 15, 2025Alexandros Kourtesakis, Eileen Bailey, Hiu Nam Hannah Chow, Hannah Rohdjeß, Normann Mussnig, Dennis Alexander Agardy, Dirk Carsten Frieder Hoffmann, Yu-Chan Chih, Rainer Will, Leon Kaulen, et al.cytokines were measured with the Human Granzyme-B DuoSet enzyme-linked immunosorbent assay (ELISA; #DY2906–05, R&D Systems) and Human IFN-gamma DuoSet ELISA (#DY285B, R&D Systems) kit. In vivo treatment with mSA2

(15) Targeting CEA in metastatic triple negative breast cancer with image-guided radiation followed by Fab-mediated chimeric antigen receptor (CAR) T-cell therapyFrontiers in ImmunologyDecember 20, 2024Eric Aniogo, Maciej Kujawski, Dennis Awuah, Seung E. Cha, Ruby Espinosa, Susanta Hui, Hemendra Ghimire, Paul J. Yazaki, Christine E. Brown, Xiuli Wang, et al.measurement Cytokine release was analyzed using an ELISA kit assay (BioLegend; 430104 and R&D systems; DY2906-05, for human IFN-γ and granzyme B respectively) according to the manufacturer’s instructions. In brief,

(16) Dynamic changes in immune cells in humanized liver metastasis and subcutaneous xenograft mouse modelsScientific reportsSeptember 2, 2024Bang HJ, Lee KH, Park MS, Sun EG, Cho SH, Chung IJ, Shim HJ, Bae WK.Duo-set; R&D Systems, Inc., Canada, USA., DY240-05), and Granzyme B (R&D Systems, Inc. Canada, USA., DY2906-05) according to the manufacturer’s instructions. All samples were measured in triplicate, and the absorbance

(17) Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservationNature communicationsMay 10, 2024Berjis A, Muthumani D, Aguilar OA, Pomp O, Johnson O, Finck AV, Engel NW, Chen L, Plachta N, Scholler J, et al.and instructions. Granzyme B ELISA Human Granzyme B DuoSet ELISA was purchased from R&D Systems (#DY2906-05). ELISA kits were used according to the manufacturer’s instructions. NK cell cryopreservation and thawing NK cells

(18) The Role of Pretreatment Serum Interleukin 6 in Predicting Short-Term Mortality in Patients with Advanced Pancreatic CancerBiomedicinesApril 18, 2024Se Jun Park, Ju Yeon Park, Kabsoo Shin, Tae Ho Hong, Younghoon Kim, In-Ho Kim, MyungAh Lee(#DY206-05), leukemia inhibitory factor (LIF) (#DYF00B), IL-1β (#DY201-05), IL-10 (#DY217B-05), granzyme B (#DY2906-05), and interferon-gamma (#DY285B-05), all sourced from R&D Systems, Minneapolis, MN, USA, and were employed

(19) Synergistic integration of histone deacetylase inhibitors apparently enhances the cytokine-induced killer cell efficiency in multiple myeloma via the NKG2D pathwayClin Transl ImmunologyMarch 25, 2024Jingjing Pu, Amit Sharma, Ting Liu, Jian Hou, Ingo Gh Schmidt-Wolfharvested, and the level of granzyme B was determined using the Human Granzyme B Elisa kit (Duoset DY2906‐05 and DY008, R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's instructions. Absorbance

(20) CAR NK92 Cells Targeting BCMA Can Effectively Kill Multiple Myeloma Cells Both In Vitro and In VivoBiomedicinesJanuary 22, 2024Park E, Mun HJ, Seo E, Hwang S, Lee JH, Song S, Sung H, Kim HY, Kwon MJ.

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Granzyme B ELISA

Human Granzyme B DuoSet ELISA

Granzyme B ELISA from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Granzyme B ELISA from R&D Systems, a Bio-Techne Brand

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