Fig 1: Differential regulation of CXCL7 (PPBP) gene expression in MDMs infected with HIV-1 or HIV-2. (A) The expression profiles of chemokine genes in MDMs infected with HIV-1 Ada or HIV-2 B5, B9, or Rod was analyzed by Affymetrix array using cell samples collected on day 15 post-infection. (B) The expression levels of CXCL7 mRNA in MDMs infected with HIV-1 Ada, 92UG024, BCF03, or Bal or HIV-2 B4, B5, B7, B8, or Rod isolates were measured by qPCR. The expression levels of CXCL7 mRNA were examined using cells harvested on day 15 post-infection, normalized by the levels of GAPDH mRNA and presented as the amount relative to uninfected cells (Cell only). Statistical analysis was performed using one-way ANOVA with Dunnett’s test for multiple testing corrections. The asterisks on the long bar line indicate a significant difference between the uninfected group and the nine HIV-1 and HIV-2-infected groups (**, p < 0.005, n = 3), as found using one-way ANOVA. The asterisks on the short bar lines indicate significant differences (*, p < 0.05) between the HIV infected group under the bar lines and the HIV-1 Ada control group, as assessed using Dunnett’s test for multiple comparisons.