MojoSort™ Mouse CD3 T Cell Isolation Kit 480024 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD3 T Cell Isolation Kit
CompanyBioLegend
Price $483.00Supplier Page View Company Product Page
Catalog Number480024
Quantity100 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD3
Cell Type (standardized)CD3+ T Cells

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Website: biolegend.com

(21) Myeloid-derived suppressor cell inhibits T-cell-based defense against Klebsiella pneumoniae infection via IDO1 productionPLoS pathogensMarch 1, 2025Xu Q, Liu X, Heng H, Wang H, Chen K, Chan EW, Yang G, Chen S.with 10% heat-inactivated FBS and were isolated with the MojoSort Mouse CD3 T Cell Isolation Kit (#480031, Biolegend). Suppression assay To analyze the proliferation of T cells, purified T cells isolated

(22) CD4+ T cell–innate immune crosstalk is critical during Staphylococcus aureus craniotomy infectionJCI InsightFebruary 24, 2025Gunjan Kak, Zachary Van Roy, Rachel W. Fallet, Lee E. Korshoj, Tammy Kieliansufficient numbers for analysis and enriched for CD3+ cells using a MojoSort Mouse CD3 Selection Kit (480031; BioLegend). Purified CD3+ cells were treated immediately ex vivo with a cocktail of PMA/ionomycin and

(23) NFAT single-deficient murine T cells reduce the risk of aGvHD while controlling cytomegalovirus infectioniScienceFebruary 21, 2025Nadine Hundhausen, Snigdha Majumder, Yin Xiao, Sigrun S. Haeusl, Helen Goehler, Rishav Seal, Cristina M. Chiarolla, Andreas Rosenwald, Matthias Eyrich, Luka Cicin-Sain, et al.cells there was the use of the Mojosort Mouse CD3 T cell Negative Isolation kit (Biolegend, cat. no. 480024) following the manufacturer’s instructions. CD4+ or CD8+ T cells were subsequently isolated using the

(24) Recognition of MR1-antigen complexes by TCR Vγ9Vδ2Frontiers in ImmunologyFebruary 18, 2025José Pedro Loureiro, Alessandro Vacchini, Giuliano Berloffa, Jan Devan, Verena Schaefer, Vladimir Nosi, Rodrigo Colombo, Aisha Beshirova, Giulia Montanelli, Benedikt Meyer, et al.collected, and CD3+ cells were enriched using the MojoSort Mouse CD3 T cell isolation kit (Biolegend, #480023) according to manufacturer instructions. T cells were rested overnight in complete medium containing

(25) A Multivalent mRNA Therapeutic Vaccine Exhibits Breakthroughs in Immune Tolerance and Virological Suppression of HBV by Stably Presenting the Pre-S Antigen on the Cell MembranePharmaceuticsFebruary 7, 2025Shang Liu, Jie Wang, Yunxuan Li, Muhan Wang, Pei Du, Zhijie Zhang, Wenguo Li, Rongchen Sun, Mingtao Fan, Meijia Yang, et al.density gradient centrifugation, and then T cells were isolated via the MagniSort Mouse CD3 T-Cell Isolation Kit (480031, BioLegend, Inc., San Diego, CA, USA) according to the manufacturer’s instructions. After counting,

(26) The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signalingNat CommunFebruary 4, 2025Naoyuki Kondo, Yuko Mimori-Kiyosue, Keizo Tokuhiro, Giuseppe Pezzotti, Tatsuo Kinashi363402), anti-mouse LFA-1 antibody (H155-78, 141002), and MojoSort Mouse CD3 T Cell Isolation Kit (480031) were from BioLegend. Ciliobrevin D (250401) was from Calbiochem. KOD-Plus-Mutagenesis Kit (SMK-101)

(27) PPARδ restrains the suppression function of intra-tumoral Tregs by limiting CIITA-MHC II expressionbioRxivDecember 21, 2024Qiyuan Yang, Yuqiong Liang, Tomoko Inoue-Hatanaka, Zhiqian Koh, Nadja Ilkenhans, Ethan Suman, Jingting Yu, Ye Zhengcells (purified by anti-PE magnetic beads system and followed by CD3 T cell isolation, Biolegend # 480024) Tumor models. Foxp3YFP-Cre, PPARδfl/flFoxp3YFP-Cre mice were injected with B16.F10 melanoma (2.5×

(28) Blockade of CCR5+ T Cell Accumulation in the Tumor Microenvironment Optimizes Anti‐TGF‐β/PD‐L1 Bispecific AntibodyAdvanced ScienceNovember 1, 2024Ming Yi, Tianye Li, Mengke Niu, Yuze Wu, Bin Zhao, Zhuoyang Shen, Shengtao Hu, Chaomei Zhang, Xiaojun Zhang, Jing Zhang, et al.isolated from the spleens of BALB/c mice using magnetic bead separation (Mouse CD3 T Cell Isolation Kit, 480024, BioLegend), labeled with CFSE (5 µm, 65‐0850‐84, ThermoFisher), and activated with anti‐CD3/CD28 (precoated

(29) HLA‐DR–Expressing Fibroblast‐Like Synoviocytes Are Inducible Antigen Presenting Cells That Present Autoantigens in Lyme ArthritisACR Open RheumatologyOctober 1, 2024Joseph R. Rouse, Rebecca Danner, Amanda Wahhab, Michaela Pereckas, Christine Nguyen, Mecaila E. McClune, Allen C. Steere, Klemen Strle, Brandon L. Jutras, Robert B. Lochheadspleens of six‐ to eight‐week‐old OT‐II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024) and stained using the Cell‐Trace CFSE cell proliferation kit (ThermoFisher C34554) according to manufacturer's

(30) Microglia LILRB4 upregulation reduces brain damage after acute ischemic stroke by limiting CD8(+) T cell recruitmentJournal of neuroinflammationAugust 31, 2024Ma Y, Zheng K, Zhao C, Chen J, Chen L, Zhang Y, Chen T, Yao X, Cai Y, Wu J.Mononuclear cell were washed and transferred to fresh RPMI-1640 medium. CD3 and CD8 negative magnetic beads (480023/480007, Biolegend, USA) were used to select the desired T cell population. Purity of the sorted cells

(31) The cell cycle regulator p16 promotes tumor infiltrated CD8(+) T cell exhaustion and apoptosisCell death & diseaseMay 15, 2024Zhang X, Wang J, Tang K, Yang Y, Liu X, Yuan S, Guo F, Zhang L, Ma K.cells were isolated from the spleens of OT-1 mice (aged 6–8 weeks) using a commercial kit (Biolegend, 480031). Then, 1 × 106 cells were seeded into each well of a 48-well plate containing 1 mL of RPMI medium supplemented

(32) LILRB3 Supports Immunosuppressive Activity of Myeloid Cells and Tumor DevelopmentCancer immunology researchMarch 4, 2024Huang R, Liu X, Kim J, Deng H, Deng M, Gui X, Chen H, Wu G, Xiong W, Xie J, et al.the tumor-bearing WT C57BL/6 mice using the Mojosort Mouse CD3 T cell Isolation Kit (Biolegend, Cat# 480024), respectively. MDSCs from tumor-bearing LILRB3 transgenic mice were cocultured with CFSE-stained T cells

(33) Naturally occurring T cell mutations enhance engineered T cell therapiesNatureFebruary 15, 2024Julie Garcia, Jay Daniels, Yujin Lee, Iowis Zhu, Kathleen Cheng, Qing Liu, Daniel Goodman, Cassandra Burnett, Calvin Law, Chloë Thienpont, et al.isolation kit (Stemcell Technologies, No. 19853) or mouse pan CD3+ T cell isolation kit (Biolegend, No. 480031). T cells were then cultured with 100 U ml−1 recombinant human IL-2 (Peprotech), anti-CD3e (1 μg ml−1)

(34) IL-35 Stabilizes Treg Phenotype to Protect Cardiac Allografts in MiceTransplantationJanuary 1, 2024Ai Huang, Kewei Liu, Ziyi Yin, Jie Liu, Hongyan Wei, Shijie Xing, Yue Qu, Lei Huang, Liancheng Li, Chang Li, et al.retrieved from C57BL/6 WT mice. CD3+ T/CD8+ T cells were sorted using CD3 magnetic beads (BioLegend, 480024)/Dynabeads Untouched Mouse CD8 Cells Kit (Invitrogen, 11417D), and flow sorting was performed to ensure

(35) Functional divergence of a bacterial enzyme promotes healthy or acneic skinNat CommunDecember 5, 2023Irshad A. Hajam, Madhusudhanarao Katiki, Randall McNally, María Lázaro-Díez, Stacey Kolar, Avradip Chatterjee, Cesia Gonzalez, Mousumi Paulchakrabarti, Biswa Choudhury, J. R. Caldera, et al.by RBC lysis (Catalog number: 00-4300-54, eBioscience™) and isolation of CD3+T cells (Catalog No.# 480031, Biolegend, USA) by negative selection using MojoSort™ Mouse CD3 T Cell Isolation Kit as per the manufacturer’s

(36) Human leukocyte antigen HLA-DR-expressing fibroblast-like synoviocytes are inducible antigen presenting cells that present autoantigens in Lyme arthritisbioRxivDecember 1, 2023Joseph R Rouse, Rebecca Danner, Amanda Wahhab, Michaela Pereckas, Mecaila E McClune, Allen C Steere, Klemen Strle, Brandon L Jutras, Robert B Lochheadisolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5–10×105

(37) Inhibition of SF3B1 improves the immune microenvironment through pyroptosis and synergizes with αPDL1 in ovarian cancerCell death & diseaseNovember 27, 2023Wang S, Liu Y, Xiao H, Chen Z, Yang X, Yin J, Li Y, Yuan C, Yan S, Chen G, et al.mouse spleen cells. T cells were separated by MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend, cat#480023) from mouse spleen cells. BMDM were separated from mouse bone marrow cells by incubation with 10 ng/mL

(38) mRNA‐based Vaccines Targeting the T‐cell Epitope‐rich Domain of Epstein Barr Virus Latent Proteins Elicit Robust Anti‐Tumor Immunity in MiceAdvanced ScienceOctober 27, 2023Ge‐Xin Zhao, Guo‐Long Bu, Gang‐Feng Liu, Xiang‐Wei Kong, Cong Sun, Zi‐Qian Li, Dan‐Ling Dai, Hai‐Xia Sun, Yin‐Feng Kang, Guo‐Kai Feng, et al.Separation Medium (Biotech, Shenzhen, China) and Mouse CD3 T Cell Isolation Kit (BioLegend, cat. # 480031) following the manufacturer's protocol. Antibodies were purified from mouse serum using rProtein G Sepharose

(39) Rap1 organizes lymphocyte front-back polarity via RhoA signaling and talin1iScienceAugust 18, 2023Ueda Y, Higasa K, Kamioka Y, Kondo N, Horitani S, Ikeda Y, Bergmeier W, Fukui Y, Kinashi T.commercial assays,Critical commercial assays ,, Mojosort Mouse CD3 T Cell Isolation Kit,BioLegend,Cat# 480031 Western Lightning Plus-ECL,PerkinElmer,Cat# NEL 104001EA ,, Experimental models: Cell lines,Experimental models

(40) Anti-phagocytosis-blocking repolarization-resistant membrane-fusogenic liposome (ARMFUL) for adoptive cell immunotherapyScience advancesAugust 9, 2023Zheng C, Zhong Q, Yi K, Kong H, Cao F, Zhuo C, Xu Y, Shi R, Ju E, Song W, et al.from the spleen via magnetic bead separation (MojoSort Mouse CD8 T Cell Isolation Kit, BioLegend, no. 480031), followed by culturing in the primary T cell culture system (iCell, no. PriMed-iCell-026). Further

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MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

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