MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

Product Specs
ItemMojoSort™ Mouse CD3 T Cell Isolation Kit
CompanyBioLegend
Price $113.00Supplier Page View Company Product Page
Catalog Number480023
Quantity10 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
TargetCD3
Cell Type (standardized)CD3+ T Cells

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A combinatorial CAAR-T cell strategy to eliminate factor VIII inhibitors in preclinical models of hemophilia AiScienceMarch 20, 2026Wenjie Li, Qirui Yang, Shuping Lai, Rongfang Wei, Lisheng Cai, Xue Zhang, Xin He, Yan ChenMojoSort™ Human CD3 T cell Isolation Kit,Biolegend,Cat#480022 MojoSort™ Mouse CD3 T cell Isolation Kit,Biolegend,Cat#480024 ImmunoCult™-XF T cell Expansion Medium,STEMCELL,Cat#10981 Cytotoxicity LDH Assay Kit-WST,DOJINDO,Cat#CK12 Human IFN-γ(Interferon Gamma) ELISA Kit

(2) Sustained monocyte activation by persistent challenges with either oxLDL or free cholesterol and underlying mechanismsImmunoHorizonsFebruary 24, 2026Yajun Wu, Shuo Geng, Grace Atkinson, Blake Caldwell, Jing Wang, Liwu Lifrom the spleens of C57BL/6 mice using the MojoSort Mouse CD3 T Cell Isolation Kit (BioLegend, no. 480031), achieving a purity greater than 95%. The isolated CD3 T cells were labeled with 5 μM carboxyfluorescein

(3) A GDF-15–GFRAL axis controls autoimmune T cell responses during neuroinflammationNature ImmunologyJanuary 15, 2026Jana K. Sonner, Audrey Kahn, Lars Binkle-Ladisch, Jan Broder Engler, Beatrice Haack, Christina Zeiler, Lisa Unger, Simone Bauer, Felix Fischbach, Giovanni Almanzar, et al.pooled, and T cells were enriched using the MojoSort Mouse CD3 or CD4 T cell Isolation Kit (BioLegend, 480024 or 480006, respectively) according to the manufacturer’s instructions. Flat-bottom cell culture plates

(4) Ubiquitous Expression of mPolgmut Leads to the Accumulation of Cytotoxic CD8+ T Lymphocytes in Young MiceLife (Basel)December 5, 2025Nadezhda A. Kuznetsova, Ksenia K. Kochegarova, Iuliia P. Baikova, Eugenii N. Korshunov, Leonid A. Ilchuk, Marina V. Kubekina, Alexandra V. Bruter, Yulia Yu. Silaeva, Ekaterina A. VarlamovaEDTA, 0.5% BSA in PBS, pH 7.2). T and B cell populations were separated using MojoSort kits (BioLegend #480024 and #480051, San Diego, CA, USA). 2.11. Evaluation of Cell Distribution by Phases of the Spermatogenic

(5) Propagation of monocyte exhaustion memory and underlying mechanismsCell Commun SignalDecember 5, 2025Jing Wang, Blake A. Caldwell, Yajun Wu, Babak Razani, Liwu Licoculture Mouse spleen CD3 + T cells were isolated using the MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend, #480031), resulting in > 95% purity. The isolated spleen CD3 + T cells were then labeled with 5 µM CFSE for

(6) IFNβ-pSTAT1-IRF1 axis controls colorectal tumor development through induction of immunogenic Ly6C+myeloid cellsNeoplasiaDecember 3, 2025Yan Li, Yanmin Wu, Liyan Liang, Xufeng Yao, Yiyang Pan, Lurong Zhang, Yaxin Wang, Jian Zhang, Kwan T Chow, Chunwan Lusorting. CD3⁺ OT-1 T cells were isolated using the MojoSortTM Mouse CD3 T Cell Isolation Kit (Cat # 480031, Biolegend) according to the manufacturer protocol from the spleens of C57BL/6-OT-1 mice. Purified CD3+

(7) CD86 costimulation enhances the antitumor activity of NKG2D CAR-Macrophages and synergizes with Anti-PD-L1 therapy to suppress prostate cancer progressionCell Communication and SignalingNovember 25, 2025Abdulrahman Ibrahim, Zihao Liang, Wan Liu, Xiaofei Di, Lawan Rabiu, Rong Li, Pengchao Zhang, Muhammad Auwal Saliu, Maoxuan Liu, Guizhong Zhang, et al.isolated from C57BL/6 mouse spleens using the MojoSort™ Mouse CD3 T Cell Isolation Kit (BioLegend, Cat# 480023) following the manufacturer’s protocol. The freshly purified T cells were then introduced into the pre-established

(8) The deubiquitinase USP17LA negatively regulates T-cell activation and attenuates anti-tumor immunityEMBO ReportsSeptember 29, 2025Huiling Zhang, Zhihan Guo, Gaigai Wei, Jingjing Yi, Zixi Wang, Yuqi Zhang, Haiping Zhao, Tingrong Ren, Yihan Wang, Jiating Kuang, et al.II,Vazyme,P213-03 PrimeSTAR Max Premix (2X),TAKARA,R045Q MojoSort™ Mouse CD3 T Cell Isolation Kit,BioLegend,480031 MojoSort™ Mouse CD4 Naive T Cell Isolation Kit,BioLegend,480040 MojoSort™ Mouse CD8 Naive T Cell Isolation

(9) Double-positive T cells form heterotypic clusters with circulating tumor cells to foster cancer metastasisJournal of Clinical InvestigationSeptember 16, 2025David Scholten, Lamiaa El-Shennawy, Yuzhi Jia, Youbin Zhang, Elizabeth Hyun, Carolina Reduzzi, Andrew D. Hoffmann, Hannah F. Almubarak, Fangjia Tong, Nurmaa K. Dashzeveg, et al.(Thermo Fisher Scientific, catalog 22363547), and counted. The MojoSort Mouse CD3 T Cell Isolation Kit (BioLegend, catalog 480024) was used to isolate T/DPT cells. Briefly, unlysed splenocytes were incubated with the biotin-antibody

(10) Secretogranin 2 binds LILRB4 resulting in immunosuppressionNature ImmunologySeptember 1, 2025Xing Yang, Ryan Huang, Meng Fang, Yubo He, Jingjing Xie, Xiaoye Liu, Chengcheng Zhang, Qi Lou, Mi Deng, Wei Xiong, et al.were isolated following the instructions for the Mojosort Mouse CD3 T cell Isolation kit (Biolegend, 480024). T cells were stained with CFSE for 10 min. Differentiated BM-MDSCs were collected and cocultured with

(11) Neutralization of acyl coenzyme A binding protein for the experimental prevention and treatment of hepatocellular carcinomaCell Rep MedJuly 15, 2025Sijing Li, Omar Motiño, Flavia Lambertucci, Jonathan Pol, Hui Chen, Long Pan, Sylvère Durand, Federica Rossin, Claudia Campani, Lucie Poupel, et al.dendritic cells (DCs) were performed using MojoSort mouse immune cell isolation kits (BioLegend, Cat: 480024, 152420, and 480097) from the spleen and lymph nodes of 11-week-old female C57BL/6J mice. Harvested

(12) Artesunate-mycophenolate Mofetil Dimer Micelles Alleviate Allogeneic Skin Graft Rejection by Inhibiting the TLR-4 Pathway in MacrophagesTheranosticsJune 12, 2025Wentao Zhao, Zhentao Yang, Hong Tang, Jintao Zheng, Zhi Liang, Ruiqi Sun, Ning Wang, Rong Su, Hangxiang Wang, Yiting Qiao, et al.T cells were isolated from spleen of male C57BL/6 CD45.1 mice via CD3+ T cells isolation kit (Cat# 480031, BioLegend). CD3+ T cells were incubated with CFSE dye in a 37 °C incubator for 10 minutes. CD3+ T cells

(13) Cbl-b inhibitor NX-1607 activates MAPK/ERK signaling pathway and enhances T-cell activationJ Immunother CancerMay 30, 2025Wenting Zhu, Shan Lu, Li Jia, Benjin Liu, Shanshan Song, Xubin Bao, Ting Yu, Yongliang Zhang, Zehong Miao, Jinxue Hespleens of C57BL/6N mice through negative selection using the MojoSort Mouse CD3 T Cell Isolation Kit (#480031, BioLegend). After euthanizing the mice, the spleen was extracted and placed in a 6-well plate with

(14) Glioma-neuronal circuit remodeling induces regional immunosuppressionNat CommunMay 22, 2025Takahide Nejo, Saritha Krishna, Akane Yamamichi, Senthilnath Lakshmanachetty, Christian Jimenez, Kevin Y. Lee, Donovan L. Baker, Jacob S. Young, Tiffany Chen, Su Su Sabai Phyu, et al.10-548E). CD3+ T-cells were isolated using the Mojosort Mouse CD3 T Cell Isolation Kit (BioLegend, 480031) and labeled with CFSE using the CFSE Cell Division Tracker Kit (BioLegend, 423801); a small fraction

(15) Comparative evaluation of CAR-expressing T-, NK-, NKT-cells, and macrophages in an immunocompetent mouse glioma modelNeuro-Oncology AdvancesMay 16, 2025Ryusuke Hatae, Payal B Watchmaker, Akane Yamamichi, Keith Kyewalabye, Kaori Okada, Su Phyu, Yitzhar Goretsky, Jeffrey Haegelin, Psalm Pineo-Cavanaugh, Marco Gallus, et al.using MojoSort™ Mouse CD3 T cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with

(16) Myeloid-derived suppressor cell inhibits T-cell-based defense against Klebsiella pneumoniae infection via IDO1 productionPLoS pathogensMarch 1, 2025Xu Q, Liu X, Heng H, Wang H, Chen K, Chan EW, Yang G, Chen S.with 10% heat-inactivated FBS and were isolated with the MojoSort Mouse CD3 T Cell Isolation Kit (#480031, Biolegend). Suppression assay To analyze the proliferation of T cells, purified T cells isolated

(17) CD4+ T cell–innate immune crosstalk is critical during Staphylococcus aureus craniotomy infectionJCI InsightFebruary 24, 2025Gunjan Kak, Zachary Van Roy, Rachel W. Fallet, Lee E. Korshoj, Tammy Kieliansufficient numbers for analysis and enriched for CD3+ cells using a MojoSort Mouse CD3 Selection Kit (480031; BioLegend). Purified CD3+ cells were treated immediately ex vivo with a cocktail of PMA/ionomycin and

(18) NFAT single-deficient murine T cells reduce the risk of aGvHD while controlling cytomegalovirus infectioniScienceFebruary 21, 2025Nadine Hundhausen, Snigdha Majumder, Yin Xiao, Sigrun S. Haeusl, Helen Goehler, Rishav Seal, Cristina M. Chiarolla, Andreas Rosenwald, Matthias Eyrich, Luka Cicin-Sain, et al.cells there was the use of the Mojosort Mouse CD3 T cell Negative Isolation kit (Biolegend, cat. no. 480024) following the manufacturer’s instructions. CD4+ or CD8+ T cells were subsequently isolated using the

(19) Recognition of MR1-antigen complexes by TCR Vγ9Vδ2Frontiers in ImmunologyFebruary 18, 2025José Pedro Loureiro, Alessandro Vacchini, Giuliano Berloffa, Jan Devan, Verena Schaefer, Vladimir Nosi, Rodrigo Colombo, Aisha Beshirova, Giulia Montanelli, Benedikt Meyer, et al.collected, and CD3+ cells were enriched using the MojoSort Mouse CD3 T cell isolation kit (Biolegend, #480023) according to manufacturer instructions. T cells were rested overnight in complete medium containing

(20) A Multivalent mRNA Therapeutic Vaccine Exhibits Breakthroughs in Immune Tolerance and Virological Suppression of HBV by Stably Presenting the Pre-S Antigen on the Cell MembranePharmaceuticsFebruary 7, 2025Shang Liu, Jie Wang, Yunxuan Li, Muhan Wang, Pei Du, Zhijie Zhang, Wenguo Li, Rongchen Sun, Mingtao Fan, Meijia Yang, et al.density gradient centrifugation, and then T cells were isolated via the MagniSort Mouse CD3 T-Cell Isolation Kit (480031, BioLegend, Inc., San Diego, CA, USA) according to the manufacturer’s instructions. After counting,

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MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD3 T Cell Isolation Kit from BioLegend

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