Deep Blue Cell Viability™ Kit from BioLegend

Product Specs
ItemDeep Blue Cell Viability™ Kit
CompanyBioLegend
Price $65.00Supplier Page View Company Product Page
Catalog Number424701
Quantity25 mL
ApplicationsFA - Quality tested
FormatAqueous solution

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Biofabricated Alginate Hydrogels to Study Prostate Tumoral Microenvironments In VitroACS OmegaMay 12, 2026Khalsa Al-Husaini, Eugenia Spessot, Esther Baena, Marco Domingos, Annalisa Tirella(H2N-PEG-Mal, PBH-943, MW: 5 kDa) was purchased from Creative PEGworks. A Deep Blue Cell Viability Kit (424701) was purchased from BioLegend. Cytopainter (ab138893) was purchased from Abcam. Puromycin (A11138–03)

(2) Human ISG15 deficiency unveils impaired healing of ulcerations via type I interferon–mediated fibrosisJournal of Human ImmunityMay 4, 2026Christos Sazeides, Lorenzo Cuollo, Ikjot Sidhu, Haley E. Randolph, Marta Martin-Fernandez, Sofija Buta, O’ Jay Stewart, Rachel Geltman, Jonas A. Adalsteinsson, Robert G. Phelps, et al.with 1,000 IU ml−1 IFN-α2b (Merck IntronA). Viability was measured with Deep Blue Cell Viability Kit (424702; BioLegend) on a Synergy H1 Hybrid Reader (BioTek). Scratch assay Fibroblasts were seeded at a cell density

(3) Artemether and Euphorbia factor L9 suppress kynurenine production through distinct effects on tryptophan metabolismThe Biochemical journalApril 8, 2026Capatina AL, Czechowski T, Plunkett-Jones C, Tonon T, Kourtzelis I, Lichman BR, Brackenbury WJ, Graham IA, Lagos D.Generon, LC Labs, Muse Chem, TRC, or VladaChem (Supplementary Figure S1). Deep Blue Cell Viability Kit (424702) was purchased from BioLegend. Human recombinant IFNγ (300-02) was purchased from PeproTech, and TNF

(4) Artemether and Euphorbia Factor L9 suppress kynurenine production through distinct effects on Tryptophan metabolismBiochemical JournalApril 8, 2026Alina L. Capatina, Tomasz Czechowski, Charlotte Plunkett-Jones, Thierry Tonon, Ioannis Kourtzelis, Benjamin R. Lichman, William J. Brackenbury, Ian A. Graham, Dimitris LagosGeneron, LC Labs, Muse Chem, TRC, or VladaChem (Supplementary Figure S1). Deep Blue Cell Viability Kit (424702) was purchased from BioLegend. Human recombinant IFNγ (300-02) was purchased from PeproTech, and TNF

(5) Pharmacological inhibition of host pathways enhances macrophage killing of intracellular bacterial pathogensMicrobiol SpectrDecember 3, 2025Ramesh Rijal, Richard H. Gomerplates (# 353072, Corning) were incubated with prewarmed Deep Blue Cell Viability resazurin dye (Cat#424702, BioLegend, San Diego, CA, USA) to a final concentration of 10% in each well (77). Plates were incubated

(6) An mRNA-based broad-spectrum antiviral inspired by ISG15 deficiency protects against viral infections in vitro and in vivoScience Translational MedicineAugust 13, 2025Yemsratch T. Akalu, Roosheel S. Patel, Justin Taft, Rodrigo Canas-Arranz, Rachel Geltman, Ashley Richardson, Sofija Buta, Marta Martin-Fernandez, Christos Sazeides, Rebecca L. Pearl, et al.24- or 48-hour incubation, viability was measured using the Deep Blue Cell Viability Kit (BioLegend, 424702). Alternatively, cells were collected and stained with Zombie NIR dye (BioLegend, 423105) or with anti–cleaved

(7) Data-Driven Sustainable In Vitro Campaigns to Decipher Invasive Breast Cancer FeaturesACS Biomaterials Science & EngineeringAugust 11, 2025Lekha Shah, Valentina Breschi, Annalisa Tirellabreast-specific microenvironments. 2.1.2 Cell Viability Alamar blue assay (Deep Blue Cell Viability Kit 424701, Biolegend) was used to analyze cell proliferation at different time points (i.e., day(s) 1, 4, 7, and

(8) Development of bioactive solid-foam scaffolds from decellularized cartilage with chondrogenic and osteogenic propertiesMaterials Today BioOctober 1, 2024Unai Mendibil, Yaiza Lópiz-Morales, Blanca Arnaiz, Raquel Ruiz-Hernández, Pablo Martín, Desiré Di-Silvio, Nerea Garcia-Urquia, Felix Elortza, Mikel Azkargorta, Beatriz Olalde, et al.replaced by warm DMEM:F12 media containing 1/10 of the Deep Blue Cell Viability reagent (Biolegend, 424701) and incubated for 4h at 37 °C and 5%CO2. Then, 100 μl of supernatant was transferred to triplicate

(9) Microfluidic-Derived Docosahexaenoic Acid Liposomes for Targeting Glioblastoma and Its Inflammatory MicroenvironmentACS Applied Materials & InterfacesAugust 7, 2024Daniel Mendanha, Marta R. Casanova, Sara Gimondi, Helena Ferreira, Nuno M. NevesAbcam, ab9659) was acquired from Abcam (Cambridge, U.K.). The Deep Blue Cell Viability Kit (Biolegend, 424702) was obtained from BioLegend (San Diego, CA). LabAssay Phospholipid (FUJIFILM Wako, LABPLIP-M1) was

(10) Inhibition of talin-induced integrin activation by a double-hit stapled peptideStructureAugust 1, 2023Tong Gao, Eun-ah Cho, Pingfeng Zhang, Jinhua WuAssays,Critical Commercial Assays,Critical Commercial Assays Deep Blue Cell Viability™ Kit 25ml,Biolegend,Cat# 424701 Deposited Data,Deposited Data,Deposited Data R7R8:S-TBS,This study,PDB: 7V1A Experimental Models:

(11) Invasion and Secondary Site Colonization as a Function of In Vitro Primary Tumor Matrix Stiffness: Breast to Bone MetastasisAdvanced Healthcare MaterialsJanuary 1, 2023Lekha Shah, Ayşe Latif, Kaye J. Williams, Elena Mancuso, Annalisa Tirellareplaced every day. 2.5 Cell Proliferation Assay Alamar blue assay (Deep Blue Cell Viability Kit, 424701, Biolegend) was used to analyze the proliferation of MDA‐MB 231 and MDA‐IV cells in biohybrid PCL scaffolds

(12) The Effect of Ca(2+) and Mg(2+) Ions Loaded at Degradable PLA Membranes on the Proliferation and Osteoinduction of MSCsPolymersJune 15, 2022Retegi-Carrión S, Ferrandez-Montero A, Eguiluz A, Ferrari B, Abarrategi A.(Blue Edition) Software and ImageJ. For measuring cell proliferation, Deep Blue Cell Viability Kit (424701; BioLegend, San Diego, CA, USA) reagent at a 10% of the total medium was added to each testing well.

(13) Human TBK1 deficiency leads to autoinflammation driven by TNF-induced cell deathCellAugust 19, 2021Taft J, Markson M, Legarda D, Patel R, Chan M, Malle L, Richardson A, Gruber C, Martín-Fernández M, Mancini GMS, et al.zVAD, and/or 5 μM MRT67307. Viability was measured with the Deep Blue Cell Viability Kit (BioLegend 424702) or CellTiter-Glo 2.0 (Promega G9241) on a Synergy H1 Hybrid Reader (BioTEK). Viral challenges Cells

(14) PLGA-particle vaccine carrying TLR3/RIG-I ligand Riboxxim synergizes with immune checkpoint blockade for effective anti-cancer immunotherapyNature communicationsMay 18, 2021Koerner J, Horvath D, Herrmann VL, MacKerracher A, Gander B, Yagita H, Rohayem J, Groettrup M.(metabolically active) cells was evaluated by the addition of 10% of Deep Blue Cell Viability™ Kit (#424701, BioLegend) according to the manufacturer’s protocol. The reduction of resazurin into resorufin was

(15) Immunostimulatory and anti-tumor metronomic cyclophosphamide regimens assessed in primary orthotopic and metastatic murine breast cancerNPJ breast cancerJanuary 1, 2020Khan KA, Ponce de Léon JL, Benguigui M, Xu P, Chow A, Cruz-Muñoz W, Man S, Shaked Y, Kerbel RS.Chemicals) for 48 h and then cell viability was assessed using Deep Blue Cell Viability kit (cat. no. 424701, BioLegend). Fluorescence intensity was measured at excitation 530 nm and emission 590 nm on a BioTek

(16) A Compendium of Mutational Signatures of Environmental AgentsCellMay 2, 2019Kucab JE, Zou X, Morganella S, Joel M, Nanda AS, Nagy E, Gomez C, Degasperi A, Harris R, Jackson SP, et al.Assays,Critical Commercial Assays,Critical Commercial Assays ,, Deep Blue Cell Viability Kit,Biolegend,Cat# 424702 ,, Deposited Data,Deposited Data,Deposited Data ,, Raw and analyzed data,This paper,EGAD00001004583 Mutation

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Deep Blue Cell Viability™ Kit from BioLegend

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Deep Blue Cell Viability™ Kit from BioLegend

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