MojoSort™ Human CD3 T Cell Isolation Kit 480022 from BioLegend

Product Specs
ItemMojoSort™ Human CD3 T Cell Isolation Kit
CompanyBioLegend
Price $594.00Supplier Page View Company Product Page
Catalog Number480022
Quantity100 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityHuman
Detection TargetCD3
Cell Type (standardized)CD3+ T Cells

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A combinatorial CAAR-T cell strategy to eliminate factor VIII inhibitors in preclinical models of hemophilia AiScienceMarch 20, 2026Wenjie Li, Qirui Yang, Shuping Lai, Rongfang Wei, Lisheng Cai, Xue Zhang, Xin He, Yan Chencommercial assays,Critical commercial assays ,, MojoSort™ Human CD3 T cell Isolation Kit,Biolegend,Cat#480022 MojoSort™ Mouse CD3 T cell Isolation Kit,Biolegend,Cat#480024 ImmunoCult™-XF T cell Expansion Medium,STEMCELL,Cat#10981 Cytotoxicity

(2) Oncostatin-M ligand-based CAR-T therapy displays robust anti-tumor activity against osteosarcomaBMC MedMarch 17, 2026Daniel Feinberg, Vinayak Uppin, Saada Eid, Claire Fritz, Masahiro Hitomi, Akshaya Radhakrishnan, Kayla Klatt, Sung Hee Choi, Jay T Myers, Aarshvi Bhatt, et al.was removed and CD3 isolation was performed utilizing the Mojosort CD3 Human T-cell Isolation Kit (480022, Biolegend). Isolated CD3 T-cells were frozen in 90% FBS and 10% DMSO in aliquots of 4–8×106 cells. Lentivirus

(3) Integrated Single-Cell Profiling Reveals Dichotomous NK Cell Populations Associated with Immunosuppression in Solid TumorsCancer Immunology ResearchFebruary 27, 2026John R. Lozada, Atef Ali, Christine Luo, Rosemary N. Plagens, Bin Zhang, Andrew Elliott, Ella Boytim, Nicholas A. Zorko, Elisabeth I. Heath, Akash Patnaik, et al.autologous T cells by negative selection using the MojoSort™ Human CD3 T Cell Isolation Kit (BioLegend, cat. 480022). NK cells were plated with 1×105 autologous T cells in a U-bottom 96 well plate at effector to target

(4) Disruption of FGL2 induces TFEB-dependent lysosomal degradation of PD-L1 and enhances the efficacy of anti-PD1 therapy in hepatocellular carcinomaCell Communication and SignalingFebruary 2, 2026Meiwen Han, Qianting Guan, Fangfei Yan, Ming Wang, Dong Xi, Qin Ningseparated from peripheral blood mononuclear cells using the MojoSort Human CD3 T Cell Isolation Kit (480022, BioLegend) and activated with 100 ng/mL CD3 antibody (317325, BioLegend), 100 ng/mL CD28 antibody (302933,

(5) CD80-Mediated T-Cell Suppression by Cancer Stem-like Cells in Head and Neck Squamous Cell CarcinomaCellsJanuary 30, 2026Mian Xiao, Lin Qiu, Qian Gao, Ruifeng Li, Jing Wang, Yanrui Feng, Xuefen Li, Xiyuan Gewere purified using a MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, San Diego, CA, USA, Cat#480022) and stimulated with anti-CD3/CD28 magnetic beads (Thermo Fisher Scientific, Waltham, MA, USA, Cat#11131D),

(6) CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signalingiScienceDecember 19, 2025Theodoros Ioannis Papadimitriou, Anne van Essen, Merel Willemse, Daphne Dorst, Elly Vitters, Birgitte Walgreen, Hans J.P.M. Koenen, Peter van der Kraan, Marije Koenders, Rogier M. Thurlings, et al.isolated from PBMCs via negative selection with magnetic-activated cell sorting (MACS) kits (Biolegend: 480022, 480010, 480012) according to manufacturer’s guidelines. Following isolation, the enriched fractions

(7) SLC7A11-specific CAR-T cell therapy potently targets colorectal and pancreatic canceriScienceNovember 21, 2025Xiaoxue Pan, Jichang Li, Jing Zhu, Xin Wang, Yucun Liu, Xiaodong Tian, Yinmo Yang, Pengyuan Wang, Shanwen Chenassays,Critical commercial assays,Critical commercial assays ,, Human CD3 T Cell Isolation Kit,BioLegend,Cat# 480022 Tumor Dissociation Kit,Miltenyi,Cat# 130-095-929 Human IL-6 ELISA Kit,Dayou,Cat# 1110602 Human TNF-α

(8) In vitro characterization of cellular responses elicited by endosomal TLR agonists encapsulated in Qβ virus-like particlesBMC ImmunologyNovember 17, 2025M. M. Hasibuzzaman, Briana Ibarra, Ishrat Nourin Khan, Thomas Craig Meagher, Caitlin D. Lemke-Miltner, George J. Weiner, Andrean L. SimonsT cells were first enriched from whole PBMCs using MojoSort™ Human CD3 T Cell Isolation Kit (BioLegend, #480022) through negative selection of the T cell population according to manufacturer’s protocol. Briefly,

(9) T-bet+CD8+ T cells govern anti-PD-1 responses in microsatellite-stable gastric cancersNat CommunApril 25, 2025Shiying Tang, Xiaofang Che, Jinyan Wang, Ce Li, Xin He, Kezuo Hou, Xiaojie Zhang, Jia Guo, Bowen Yang, Danni Li, et al.the biotin antibody cocktail, followed by incubation with magnetic Streptavidin Nanobeads (Biolegend, 480022, 480012). Untouched CD3+ or CD8+ cells were collected using a magnetic separator (StemCell Technologies,

(10) Glia Modulates Immune Responses in the Retina Through Distinct MHC PathwaysGliaJanuary 28, 2025Simona Intonti, Despina Kokona, Martin S. Zinkernagel, Volker Enzmann, Jens V. Stein, Federica M. Conederafalls within the range of 1 × 107 to 2 × 108 for using the MojoSort human CD3 T‐cell isolation kit (480022; BioLegend). The procedure was performed as recommended by the manufacturer. In brief, 10 μL of the

(11) YAP1 Inhibition Induces Phenotype Switching of Cancer-Associated Fibroblasts to Tumor Suppressive in Prostate CancerCancer ResearchNovember 15, 2024Hongtao Song, Tong Lu, Donghui Han, Jiayu Zhang, Lunbiao Gan, Chao Xu, Shaojie Liu, Peng Li, Keying Zhang, Zhihao Hu, et al.peripheral blood mononuclear cells (PBMC) by using the Human CD3+ T-cell Isolation Kit (BioLegend, #480022) and cultured in RPMI-1640 containing 10% FBS. Human primary CAFs were dissociated from tumor samples

(12) Adenoviral delivery of the CIITA transgene induces T‐cell‐mediated killing in glioblastoma organoidsMolecular OncologyNovember 13, 2024Ilaria Salvato, Eliane Klein, Aurélie Poli, Mahsa Rezaeipour, Luca Ermini, Bakhtiyor Nosirov, Anuja Lipsa, Anaïs Oudin, Virginie Baus, Gian Mario Dore, et al.based on their HLA matching (Table S1). T‐cell populations were isolated from PBMCs using CD3+ (Cat# 480022; BioLegend, Amsterdam, The Netherlands), CD4+ (Cat# 130‐096‐533; Miltenyi, Leiden, The Netherlands)

(13) Cancer Cell Small Molecule Secretome Induces the Immune Checkpoint NKG2A and Dysfunction of Human CD8+ T CellsImmunoHorizonsJune 1, 2024Rodriguez-Garcia GJ, Graves DK, Mirza MB, Idrees K, Kim YJ, Korrer MJ, Rathmell JC.human CD8+ T cell isolation kit (130-096-495) or MojoSort human CD8 T cell isolation kit (BioLegend, 480022) according to the manufacturers’ instructions. All human CD8+ T cells were cultured at 37°C with 5%

(14) DPSCs regulate epithelial-T cell interactions in oral submucous fibrosisStem cell research & therapyApril 23, 2024Wang SY, Zhang SJ, Meng HF, Xu HQ, Guo ZX, Yan JF, Gao JL, Niu LN, Wang SL, Jiao K.buffer and subjected to sorting using the MojoSort Human CD3 T Cell Isolation Kit (BioLegend; Cat. no. 480022). To sort the CD3 + T cells, 10 µL of CD3 negative antibody was added to each 1 × 107 cells. Once sorted,

(15) Patient-Specific Targeting of the T-Cell Receptor Variable Region as a Therapeutic Strategy in Clonal T-Cell DiseasesClinical cancer research : an official journal of the American Association for Cancer ResearchOctober 13, 2023Lucero OM, Lee JA, Bowman J, Johnson K, Sapparapu G, Thomas JK, Fan G, Chang BH, Thiel-Klare K, Eide CA, et al.ice. Antibody-dependent cellular cytotoxicity. Patient J T-LGL CD3+ T-cells, isolated with a magnetic column (Biolegend, 480022), were plated at 3E4 cells/well in a U-bottom 96-well plate (Falcon, 353077).[28] Cells were opsonized

(16) Double Staining with Fluorescent Tracers to Determine Myeloid Cell Migration of Leishmania-infected Cells from Mouse Skin to Lymphatic Tissues by Flow CytometryBio-protocolSeptember 20, 2023Uscanga-Palomeque AC, Osorio YE, Melby PC.

(17) Inulin fibre promotes microbiota-derived bile acids and type 2 inflammationNatureNovember 1, 2022Arifuzzaman M, Won TH, Li TT, Yano H, Digumarthi S, Heras AF, Zhang W, Parkhurst CN, Kashyap S, Jin WB, et al.

(18) Size-dependent activation of CAR-T cellsScience immunologyAugust 5, 2022Xiao Q, Zhang X, Tu L, Cao J, Hinrichs CS, Su X.from PBMCs (Zenbio #SER-PBMC-200) using MojoSort™ Human CD3 or CD8 T Cell Isolation Kit (BioLegend, #480022 or #480012) and activated with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher, #11161D). Cells were

(19) CF33-hNIS-antiPDL1 virus primes pancreatic ductal adenocarcinoma for enhanced anti-PD-L1 therapyCancer gene therapyJune 1, 2022Zhang Z, Yang A, Chaurasiya S, Park AK, Lu J, Kim SI, Warner SG, Yuan YC, Liu Z, Han H, et al.

(20) Immunostimulatory Cancer-Associated Fibroblast Subpopulations Can Predict Immunotherapy Response in Head and Neck CancerClinical Cancer ResearchMay 13, 2022Aleksandar Obradovic, Diana Graves, Michael Korrer, Yu Wang, Sohini Roy, Abdullah Naveed, Yaomin Xu, Adam Luginbuhl, Joseph Curry, Michael Gibson, et al.isolated from PBMCs using magnetic bead sort with the MojoSort Human CD3 T Cell Isolation Kit (Biolegend #480022) according to manufacturer’s instructions. For isolation of CD3+ tumor-infiltrating lymphocytes (TILs),

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(1)

6/9/2023Easy to Use Kit for Sorting Cells Soheil TavakolpourThe MojoSort™ Human CD3 T Cell Isolation Kit is a specialized kit used to separate CD3-positive T cells from other cell types in human samples. It employs magnetic beads to achieve efficient and ... Read Review

Four Key Considerations When Performing Magnetic Cell Separation
February 02, 2016
The separation of specific cells within a complex cell mixture is a challenge that researchers face on a daily basis. Three major techniques have emerged as most widely used: centrifugation-based, fluorescence-activated ... read more

MojoSort™ Human CD3 T Cell Isolation Kit from BioLegend

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Four Key Considerations When Performing Magnetic Cell Separation

MojoSort™ Human CD3 T Cell Isolation Kit from BioLegend

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