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Assay Kit
MojoSort(TM) Mouse CD4 T Cell Isolation Kit
MojoSort(TM) Mouse CD4 T Cell Isolation Kit from BioLegend
(2)
Write a review
Citations:
(24)
Supplier Page
Supplier Page from
BioLegend for
MojoSort(TM) Mouse CD4 T Cell Isolation Kit
Description
MojoSort(TM) Mouse CD4 T Cell Isolation Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MojoSort(TM) Mouse CD4 T Cell Isolation Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
480005
Quantity
10 tests
Applications
Cell Sep - Neg
Format
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
Reactivity
Mouse
Detection Target
CD4
Cell Type (standardized)
CD4+ T Cell
Conjugate/Tag/Label
MOJO
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BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (24)
(1)
Plasmacytoid dendritic cells contribute to vascular endothelial dysfunction in type 2 diabetes
Frontiers in Cardiovascular Medicine
November 29, 2023
K. Alluri, B. Srinivas, S. Belmadani, K. Matrougui
and db/db
mice
using a CD4
T cell
isolation kit from
Biolegend
as per manufacturer protocol (cat #
480006
, San Diego, CA, United States) and cultured in RPMI 1640 with 1% penicillin-streptomycin. 1% Glutamax
(2)
Noninvasive ultrasound stimulation to treat myocarditis through splenic neuro-immune regulation
Journal of Neuroinflammation
April 17, 2023
Tianshu Liu, Yanan Fu, Jiawei Shi, Shukun He, Dandan Chen, Wenqu Li, Yihan Chen, Li Zhang, Qing Lv, Yali Yang,
et al.
into single-cell suspensions. CD4+
T cell
or CD11b+
macrophage
was selected using an isolation kit (#
480005
and #
480109
,
Biolegend
, USA). Total RNA was extracted using
TRIzol Reagent
according to the manufacturer’s
(3)
Characterization of proteogenomic signatures of differentiation of CD4+ T cell subsets
DNA Research
February 1, 2023
Toshio Kanno, Ryo Konno, Keisuke Miyako, Takahiro Nakajima, Satoru Yokoyama, Shigemi Sasamoto, Hikari K Asou, Junichiro Ohzeki, Yusuke Kawashima, Yoshinori Hasegawa,
et al.
were obtained by the negative selection using the Mojo Sort Mouse CD4 T Cell Isolation Kit (
Biolegend
#
480006
) and positive selection using
CD62L MicroBeads, mouse
(
Miltenyi Biotec
#
130-049-701
). Naïve CD4+ T cells
(4)
A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γ
Cell reports
November 22, 2022
Tang X, Arora G, Matias J, Hart T, Cui Y, Fikrig E.
were isolated from splenocytes with MojoSort™
mouse
CD4 and
CD8
T cell
isolation kits (
BioLegend
, #
480005
and #
480007
). The purity of the isolated cells was evaluated by
flow cytometry
with the antibodies of
(5)
Distinctive populations of CD4+T cells associated with vaccine efficacy
iScience
September 16, 2022
Therese Woodring, Colin N. Dewey, Lucas Dos Santos Dias, Xin He, Hannah E. Dobson, Marcel Wüthrich, Bruce Klein
1% FBS) and the CD4+T cells were enriched using MojoSort™
Mouse
CD4
cell isolation
(
Biolegend
, Cat#
480006
). The cells were stained and sorted as explained before in
scRNAseq
section as well. Both lungs and
(6)
FOXP3 exon 2 controls T(reg) stability and autoimmunity
Science immunology
June 24, 2022
Du J, Wang Q, Yang S, Chen S, Fu Y, Spath S, Domeier P, Hagin D, Anover-Sombke S, Haouili M,
et al
.
Foxp3
FL (WT);DEREG or
Foxp3
ΔE2;DEREG
mice
with MoJoSort mouse CD4 T cell isolation kit (
BioLegend
,
480006
). Enriched CD4 T cells were then stained with anti-mouse CD4 and Tregs were sorted based upon eGFP expression
(7)
SCD2-mediated cooperative activation of IRF3-IRF9 regulatory circuit controls type I interferon transcriptome in CD4(+) T cells
Frontiers in immunology
January 1, 2022
Kanno T, Miyako K, Nakajima T, Yokoyama S, Sasamoto S, Asou HK, Ohara O, Nakayama T, Endo Y.
were obtained by the negative selection using the Mojo Sort Mouse CD4 T Cell Isolation Kit (
Biolegend
#
480006
) and positive selection using
CD62L MicroBeads, mouse
(
Miltenyi Biotec
#
130-049-701
). Naïve CD4+ T cells
(8)
Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes
The Journal of experimental medicine
November 1, 2021
Lin CH, Chen MC, Lin LL, Christian DA, Min B, Hunter CA, Lu LF.
Next, CD4+ IELs were further isolated by using
Mouse
CD4
T Cell
Negative Selection kit (
BioLegend
;
480006
). Finally, isolated CD4+ IELs were stained with anti-CD8α-PE antibody, and CD8αα+CD4+ IELs were separated
(9)
BFAR coordinates TGFβ signaling to modulate Th9-mediated cancer immunotherapy
The Journal of experimental medicine
July 5, 2021
Pei S, Huang M, Huang J, Zhu X, Wang H, Romano S, Deng X, Wang Y, Luo Y, Hao S,
et al
.
(10)
Enhanced Susceptibility of Galectin-1 Deficient Mice to Experimental Colitis
Frontiers in Immunology
June 28, 2021
Raquel Fernandez-Perez, Mercedes Lopez-Santalla, Rebeca Sánchez-Domínguez, Omaira Alberquilla, Irene Gutiérrez-Cañas, Yasmina Juarranz, Juan A. Bueren, Marina I. Garin
(11)
Pairwise Stimulations of Pathogen-Sensing Pathways Predict Immune Responses to Multi-adjuvant Combinations
Cell Systems
November 1, 2020
Surya Pandey, Adam Gruenbaum, Tamara Kanashova, Philipp Mertins, Philippe Cluzel, Nicolas Chevrier
cell suspension by positive selection using the MojoSort Mouse CD4 T Cell Isolation Kit (
BioLegend
480006
). Cytokine quantifications.
Cell culture
supernatants were collected from (1) BMDC (100,000 cells/well)
(12)
Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung Cancer
Cancer immunology research
November 1, 2020
Qin J, Zhang X, Tan B, Zhang S, Yin C, Xue Q, Zhang Z, Ren H, Chen J, Liu M,
et al
.
(13)
PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction
Cell reports
October 20, 2020
Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, Wang L, Jiang Y, Clay R, Wei X,
et al
.
cell Isolation Kit,
Biolegend
,Cat #
480035
MojoSort Mouse CD4 T cell Isolation Kit,
Biolegend
,Cat #
480005
10* Permeabilization buffer,
eBioscience
,Cat #
00-8333-56
Fix/Permeabilization buffer set,eBioscience,Cat
(14)
Self-Assembled Multivalent Aptamer Nanoparticles with Potential CAR-like Characteristics Could Activate T Cells and Inhibit Melanoma Growth
Molecular therapy oncolytics
June 26, 2020
Bai C, Gao S, Hu S, Liu X, Li H, Dong J, Huang A, Zhu L, Zhou P, Li S,
et al
.
with a nylon hair column (Polyscience, 21759-1) or a
mouse
CD4/CD8
T cell
isolation kit (
Biolegend
,
480005
/
480007
) and added to 1640 medium for subsequent experiments.
T cells
were then stained with CFDA-SE
(15)
Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages
PLoS pathogens
May 1, 2020
Cheng Y, Kiene NJ, Tatarian A, Eix EF, Schorey JS.
infection
as described above).
T cells
were isolated using MojoSort™ Mouse CD4+ T Cell Isolation Kit (Cat.
480005
,
Biolegend
) and MojoSort™ and mouse CD8+ T Cell Isolation Kit (Cat.
480007
,
Biolegend
). Purified CD4+
(16)
T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity
Nature immunology
January 1, 2020
Jain A, Irizarry-Caro RA, McDaniel MM, Chawla AS, Carroll KR, Overcast GR, Philip NH, Oberst A, Chervonsky AV, Katz JD,
et al
.
506312
for detection), Naïve
T cell
Mojosort (
Biolegend
;
480040
), total CD4+
T cell
Mojosort (
Biolegend
;
480006
), Zombie yellow (
Biolegend
;
423103
),
DAPI
(
Biolegend
;
422801
), IETD (
R&D Systems
;
FMK007
, 10μM), rGMCSF
(17)
CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model
Frontiers in immunology
January 1, 2020
Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, Li J, Liang S, Xiong J, Li Z,
et al
.
from Cd226−/− or WT
mice
were negatively selected using the Mojosort™ Mouse CD4 T Cell Isolation kit (
480006
,
Biolegend
, San Diego, CA, USA). The purified CD4+ T cells (cell purity >95%) were cultured in RPMI
(18)
Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro
Scientific reports
November 22, 2019
Nomura S, Takahashi H, Suzuki J, Kuwahara M, Yamashita M, Sawasaki T.
(19)
Cross-Species Single-Cell Analysis of Pancreatic Ductal Adenocarcinoma Reveals Antigen-Presenting Cancer-Associated Fibroblasts
Cancer discovery
August 1, 2019
Elyada E, Bolisetty M, Laise P, Flynn WF, Courtois ET, Burkhart RA, Teinor JA, Belleau P, Biffi G, Lucito MS,
et al
.
enriched for CD4+ T cells by negative selection using MojoSort mouse CD4 T cell isolation kit (
Biolegend
#
480006
). APC/CAF plates were washed twice, 50,000 CD4+ T cells were co-plated in 10% FBS/DMEM in each well,
(20)
Uterine B Cells Exhibit Regulatory Properties During the Peri-Implantation Stage of Murine Pregnancy
Frontiers in immunology
January 1, 2019
Guzman-Genuino RM, Eldi P, Garcia-Valtanen P, Hayball JD, Diener KR.
Page
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Reviews
Write a Review
(1)
1/31/2020
CD4 Enrichment of Cultured Splenocytes Using MojoSort
Troy Trevino
The MojoSort Mouse CD4 T cell isolation kit works very well to enrich for CD4 cells in a primary splenocyte culture. When used in tandem with a dead cell separation medium like Lympholyte-M, high ...
Read Review
(1)
12/27/2019
Pan CD4 T Cell Isolation Kit from Mouse
Wei Xu
You can use this kit to get acceptable yield and purity to isolate CD4 cells from mouse with reasonable price.
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MojoSort(TM) Mouse CD4 T Cell Isolation Kit from BioLegend
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