MojoSort™ Mouse CD4 T Cell Isolation Kit 480005 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD4 T Cell Isolation Kit
CompanyBioLegend
Price $113.00Supplier Page View Company Product Page
Catalog Number480005
Quantity10 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD4
Cell Type (standardized)CD4+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

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Website: biolegend.com

(1) GM-CSF promotes pro-inflammatory macrophage activation associated with Akt/mTOR signaling during experimental colitisFrontiers in ImmunologyJune 26, 2026Silan Shen, Kexin Chen, Lili Li, Mingshan Jiang, Yongbin Jia, Xiufeng Bai, Zhen Zeng, Chunxiang Ma, Yuan Dang, Kehan Hu, et al.spleen was immediately removed from the sacrificed mice, and a MojoSort™ Mouse CD4 T Cell Isolation Kit (480006, BioLegend) was used to isolate CD4+ T cells according to the instructions. Twenty-four-well plates

(2) Ligature-induced periodontitis in mice potentially accelerates CD4+ T-cell senescence and exacerbates rheumatoid arthritisFrontiers in ImmunologyMay 26, 2026Jinfeng Li, Terukazu Sanui, Miyu Shida, Karen Yotsumoto, Mwannes Ahmad, Ziyu Wang, Meng Xiao, Chikako Hayashi, Takanori Shinjo, Takaharu Taketomi, et al.were subjected to negative selection using the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend, #480006) according to the manufacturer’s instructions. The purity of isolated CD4+ T cells was 95%–98%, as confirmed

(3) Ferroptotic tumor cells reprogram tumor-associated macrophage antigen presentation to enhance the efficacy of immune checkpoint blockadeCell Rep MedMay 19, 2026Jia-Lei Sun, Yong-Chao Chu, Fu Wang, Ding-Dang Yu, Jin-Rui Liu, Ru-Chen Xu, Hua-Hua Liu, Zhuo-Ran Qi, Xuan Shi, Xiang-Nan Yu, et al.kit,Beyotime,Cat# S0053 NADP+/NADPH assay kit,Beyotime,Cat# S0179 Mouse CD4 T cell Isolation Kit,BioLegend,Cat# 480005 Vitamin A ELISA Kit,Elabsciencee,Cat# E-EL-0135 ,, Deposited data,Deposited data,Deposited data ,, Raw

(4) The long noncoding RNA lnc13 restrains inflammatory responses to maintain oral tolerance to glutenNature ImmunologyApril 30, 2026Ruxiao Yang-Fischer, Alyssa Shearer, Felipe Batista Leão, Izei Pascual-Gonzalez, Arnold Han, Ainara Castellanos-Rubio, Govind Bhagat, Sankar Ghoshlymph nodes. Peripheral CD4+ T cells were isolated with MojoSort Mouse CD4+ T cell Isolation kit (BioLegend 480005). CD8+ T cells were cultured in RPMI-1640 containing 10% heat-inactivated FBS (Peak Serum PS-SB4), 1×

(5) Canonical WNT Ligands Produced by Regulatory T Cells Restrain Effector CD4 + T Cell ResponsesbioRxivApril 17, 2026Kashish Singh Singh Parihar, Margaret R. Niemeier, Igal Ifergan(StemCell Technologies; cat# 19852RF) or the MojoSort CD4+ T Cell Negative Isolation Kit (BioLegend; cat# 480005), following the manufacturers’ protocols, and further enriched for naïve CD4+ T cells via positive selection

(6) Non-canonical IL‑22 receptor signaling remodels the oral mucosal barrier during Candida albicans immunosurveillanceNature CommunicationsApril 3, 2026Nicolas Millet, Jinendiran Sekar, Norma V. Solis, Jian Miao, Antoine Millet, Felix E. Y. Aggor, Asia Wildeman, Melissa E. Cook, Amirhossein Davari, Brian M. Peters, et al.washing, negative CD4 T cell selection was performed using MojoSort™ Mouse CD4 T Cell Isolation Kit (#480006; BioLegend). Post-enrichment purity of CD4 T cells was >97.1%. 1 × 106 CD4 T cells were injected intravenously

(7) Mitochondrial transfer to granulocytic myeloid-derived suppressor cells augments immunosuppressive activityCell ReportsMarch 24, 2026Prabhakar Arumugam, Cortney E. Heim, Rachel W. Fallet, Dhananjay D. Shinde, Vinai C. Thomas, Rafael J. Argüello, Tammy Kielian,Zymo Research,R1050 Wizard Genomic DNA Purification Kit,Promega,A1120 MojoSort Mouse CD4 T cell Isolation Kit,BioLegend,480006 ELISA MAX Standard Set Mouse MCP-1,BioLegend,432701 Foxp3/Transcription Factor Staining Buffer Kit,Tonbo Biosciences

(8) Targeting the Mapk13‐Tcf1‐Slc7a5 Axis via One‐Carbon Metabolic Regulation to Prevent Chronic Allograft VasculopathyAdvanced ScienceMarch 1, 2026Wang Yi, Di Wu, Jing Liu, Shi Chen, Liu Song, Bin Xie, Aini Xie, Peixiang Lan, Zhishui ChenT‐Cell Stimulation Splenic CD4+ T cells were isolated using the Mouse CD4 T Cell Isolation Kit (cat# 480005, BioLegend) according to the manufacturer's protocol. To stimulate T cells, naive CD4+ T cells were

(9) Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatosNature NeuroscienceFebruary 17, 2026Jackson W. Mace, Sachin P. Gadani, Matthew D. Smith, Danny Galleguillos, Bong Gu Kang, Milton Roy, Meilian Liu, Benjamin Summers, Thomas Garton, Marjan Gharagozloo, et al.negative selection magnetic bead kit following the manufacturer’s recommendation (BioLegend, cat. no. 480005). T cells were counted and labeled with CellTrace Violet (Cell Signaling, cat. no. 48444). Cells were

(10) A GDF-15–GFRAL axis controls autoimmune T cell responses during neuroinflammationNature ImmunologyJanuary 15, 2026Jana K. Sonner, Audrey Kahn, Lars Binkle-Ladisch, Jan Broder Engler, Beatrice Haack, Christina Zeiler, Lisa Unger, Simone Bauer, Felix Fischbach, Giovanni Almanzar, et al.T cells were enriched using the MojoSort Mouse CD3 or CD4 T cell Isolation Kit (BioLegend, 480024 or 480006, respectively) according to the manufacturer’s instructions. Flat-bottom cell culture plates were coated

(11) A Brain Endothelial Cell Caveolin-1/CXCL10 Axis Promotes T Cell Transcellular Migration Across the Blood-Brain BarrierASN NeuroDecember 31, 2025Troy N. Trevino, Ali A. Almousawi, Remy Martins-Goncalves, Andrea Ochoa-Raya, KaReisha F. Robinson, Genesis L. Abad, Leon M. Tai, Suellen D. Oliveira, Richard D. Minshall, Sarah E. Lutzanother 2 days (Lutz et al., 2017). After 5 days in vitro, MojoSort CD4 selection beads (Biolegend 480005) were used to select CD4+ T cells. We used a flow cytometry approach to verify the purification of CD4+

(12) BRD4 Inhibitor Alleviates Recurrent Spontaneous Abortion via Regulating BRD4/STAT3/IL-17A Axis to Decrease the Th17 Cell DifferentiationReprod Med BiolOctober 15, 2025Fen Liu, Zhenhui Zhang, Chengying Yangsingle‐cell suspensions. CD4+ T cells were purified using the CD4+ T cell isolation kit (Biolegend, 480006, USA). The 96‐well plates were covered with anti‐CD3 (Elabscience, E‐AB‐F1013C, China) and incubated

(13) Targeting HK3 in tumor-associated macrophages enhances antitumor immunity through augmenting antigen cross-presentation in cervical cancerJ Immunother CancerJuly 15, 2025Yifan Yang, Xun Tian, Jinglan Chen, Jian Liu, Han Jiang, Liting Liu, Shen Qu, Min Wu, Jingyu Wang, Ying Chen, et al.assay Mouse naïve CD8+ T cells and CD4+ T cells from spleen were extracted with an isolation kit (480044 or 480005, BioLegend). BMDMs were pulsed with ovalbumin (OVA) (100 µg/mL, vac-stova, InvivoGen) overnight in advance.

(14) The OGT–TFF2 axis mediates intrahepatic crosstalk and MASH pathogenesisHepatologyJune 30, 2025Lei Zhang, Cuijuan Han, Man Mohan Shrestha, Jiamei Le, Wilhelm K. Berger, Yiheng Huang, Reina Desrouleaux, Eric Wang, Laszlo Nagy, Xiaoyong YangSimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads),Cell Signaling Technology,9003S MojoSort™ Mouse CD4 T Cell Isolation Kit,Biolegend,480006 MojoSort™ Mouse CD8 naïve T Cell Isolation Kit,Biolegend,480043 MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit

(15) The OGT-TFF2 axis mediates intrahepatic crosstalk and MASH pathogenesisHepatologyJune 30, 2025Lei Zhang, Cuijuan Han, Man Mohan Shrestha, Jiamei Le, Kristian Berger, Yiheng Huang, Reina Desrouleaux, Eric Wang, Laszlo Nagy, Xiaoyong YangSimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads),Cell Signaling Technology,9003S MojoSort™ Mouse CD4 T Cell Isolation Kit,Biolegend,480006 MojoSort™ Mouse CD8 naïve T Cell Isolation Kit,Biolegend,480043 MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit

(16) Hepatitis B virus promotes liver cancer by modulating the immune response to environmental carcinogensNature communicationsJune 27, 2025Huang M, Wang D, Huang J, Bae AN, Xia Y, Zhao X, Mortaja M, Azin M, Collier MR, Semenov YR, et al.CD4+ T cells were isolated using MojoSort mouse CD4+ T cells isolation kits (Biolegend, catalog no. 480006). After cell counting, 5 × 106 cells were resuspended in 1 mL of R10 media containing 2 μg/mL of CD28

(17) Fibroblast lipid metabolism through ACSL4 regulates epithelial sensitivity to ferroptosis in IBDNature MetabolismJune 26, 2025Wesley Huang, Yuezhong Zhang, Nupur K. Das, Sumeet Solanki, Chesta Jain, Marwa O. El-Derany, Imhoi Koo, Hannah N. Bell, Noora Aabed, Rashi Singhal, et al.C57BL/6 mice were isolated from the spleen using a MojoSort mouse CD4 T Cell Isolation Kit (BioLegend, 480005) and sorted by flow cytometry using a 1:100 dilution of an anti-mouse CD4 PE-Cy7 antibody (Invitrogen,

(18) A DNA Vaccine Against Proadrenomedullin N-Terminal 20 Peptide (PAMP) Reduces Angiogenesis and Increases Lymphocyte and Macrophage Infiltration but Has No Effect on Tumor Burden in a Mouse Model of Lung MetastasisVaccines (Basel)May 30, 2025Tom Kalathil Raju, Srdan Tadic, Pablo Garrido, Laura Ochoa-Callejero, Judit Narro-Íñiguez, Josune García-Sanmartín, Alfredo Martínez1.0 × 106 cells/mL. Then, CD4+ lymphocytes were purified with the MojoSort CD4 T Cell Isolation Kit (480005, BioLegend) following the manufacturer’s instructions. The isolated T cells were kept in RPMI supplemented

(19) Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunitySci AdvApril 18, 2025Keisuke Imabayashi, Yutaro Yada, Kazuhiko Kawata, Motoki Yoshimura, Takeshi Iwasaki, Akemi Baba, Akihito Harada, Koichi Akashi, Hiroaki Niiro, Yoshihiro Babafrom bm12 mice by negative selection using the MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend, 480005), were transferred into the recipient mice. On days 13 and 14 after B cell transfer, diphtheria toxin

(20) CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cellsElifeApril 4, 2025Xiaoyu Meng, Yezhang Zhu, Kuai Liu, Yuxi Wang, Xiaoqian Liu, Chenxin Liu, Yan Zeng, Shuai Wang, Xianzhi Gao, Xin Shen, et al.Foxp3YFP-Cre and Foxp3YFP-CreCxxc1fl/fl mice were enriched using the Mouse CD4 T Cell Isolation Kit (480005; BioLegend) and then sorted using the BD Aria II flow cytometer. Naive CD4+T cells from 2D2 (MOG35-55-specific

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(1)

1/31/2020CD4 Enrichment of Cultured Splenocytes Using MojoSort Troy TrevinoThe MojoSort Mouse CD4 T cell isolation kit works very well to enrich for CD4 cells in a primary splenocyte culture. When used in tandem with a dead cell separation medium like Lympholyte-M, high ... Read Review
(1)

12/27/2019Pan CD4 T Cell Isolation Kit from Mouse Wei XuYou can use this kit to get acceptable yield and purity to isolate CD4 cells from mouse with reasonable price.

MojoSort™ Mouse CD4 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD4 T Cell Isolation Kit from BioLegend

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