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Assay Kit
MojoSort™ Mouse CD4 T Cell Isolation Kit
MojoSort™ Mouse CD4 T Cell Isolation Kit from BioLegend
(2)
Write a review
Citations:
(58)
Supplier Page
Supplier Page from
BioLegend for
MojoSort™ Mouse CD4 T Cell Isolation Kit
Description
MojoSort™ Mouse CD4 T Cell Isolation Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MojoSort™ Mouse CD4 T Cell Isolation Kit
Company
BioLegend
Price
$483.00
Supplier Page
View Company Product Page
Catalog Number
480006
Quantity
100 tests
Applications
Cell Separation (MojoSort™, ) - Quality tested
Format
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
Reactivity
Mouse
Detection Target
CD4
Cell Type (standardized)
CD4+ T Cell
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (58)
(1)
Mitochondrial transfer to granulocytic myeloid-derived suppressor cells augments immunosuppressive activity
Cell Reports
March 24, 2026
Prabhakar Arumugam, Cortney E. Heim, Rachel W. Fallet, Dhananjay D. Shinde, Vinai C. Thomas, Rafael J. Argüello, Tammy Kielian
,
Zymo Research
,
R1050
Wizard Genomic DNA Purification Kit
,
Promega
,
A1120
MojoSort Mouse CD4 T cell Isolation Kit
,
BioLegend
,
480006
ELISA MAX Standard Set Mouse MCP-1
,
BioLegend
,
432701
Foxp3/Transcription Factor Staining Buffer Kit
,
Tonbo Biosciences
(2)
Targeting the Mapk13‐Tcf1‐Slc7a5 Axis via One‐Carbon Metabolic Regulation to Prevent Chronic Allograft Vasculopathy
Advanced Science
March 1, 2026
Wang Yi, Di Wu, Jing Liu, Shi Chen, Liu Song, Bin Xie, Aini Xie, Peixiang Lan, Zhishui Chen
T‐Cell
Stimulation Splenic CD4+ T cells were isolated using the Mouse CD4 T Cell Isolation Kit (cat#
480005
,
BioLegend
) according to the manufacturer's protocol. To stimulate
T cells
, naive CD4+ T cells were
(3)
Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos
Nature Neuroscience
February 17, 2026
Jackson W. Mace, Sachin P. Gadani, Matthew D. Smith, Danny Galleguillos, Bong Gu Kang, Milton Roy, Meilian Liu, Benjamin Summers, Thomas Garton, Marjan Gharagozloo,
et al.
negative selection magnetic bead kit following the manufacturer’s recommendation (
BioLegend
, cat. no.
480005
).
T cells
were counted and labeled with CellTrace
Violet
(
Cell Signaling
, cat. no.
48444
). Cells were
(4)
A GDF-15–GFRAL axis controls autoimmune T cell responses during neuroinflammation
Nature Immunology
January 15, 2026
Jana K. Sonner, Audrey Kahn, Lars Binkle-Ladisch, Jan Broder Engler, Beatrice Haack, Christina Zeiler, Lisa Unger, Simone Bauer, Felix Fischbach, Giovanni Almanzar,
et al.
T cells
were enriched using the MojoSort
Mouse
CD3 or CD4
T cell
Isolation Kit (
BioLegend
,
480024
or
480006
, respectively) according to the manufacturer’s instructions. Flat-bottom
cell culture
plates were coated
(5)
A Brain Endothelial Cell Caveolin-1/CXCL10 Axis Promotes T Cell Transcellular Migration Across the Blood-Brain Barrier
ASN Neuro
December 31, 2025
Troy N. Trevino, Ali A. Almousawi, Remy Martins-Goncalves, Andrea Ochoa-Raya, KaReisha F. Robinson, Genesis L. Abad, Leon M. Tai, Suellen D. Oliveira, Richard D. Minshall, Sarah E. Lutz
another 2 days (Lutz et al., 2017). After 5 days
in vitro
, MojoSort CD4 selection beads (
Biolegend
480005
) were used to select CD4+ T cells. We used a
flow cytometry
approach to verify the
purification
of CD4+
(6)
BRD4 Inhibitor Alleviates Recurrent Spontaneous Abortion via Regulating BRD4/STAT3/IL-17A Axis to Decrease the Th17 Cell Differentiation
Reprod Med Biol
October 15, 2025
Fen Liu, Zhenhui Zhang, Chengying Yang
single‐cell suspensions. CD4+ T cells were purified using the CD4+
T cell
isolation kit (
Biolegend
,
480006
, USA). The 96‐well plates were covered with anti‐
CD3
(
Elabscience
,
E‐AB‐F1013C
, China) and incubated
(7)
Phosphoglycerate mutase regulates Treg differentiation through control of serine synthesis and one-carbon metabolism
eLife
July 28, 2025
Godfrey WH, Lee JJ, Shanmukha S, Cho K, Deng X, Ambati CSR, Putluri V, Mostafa Kamal AH, Kim PM, Putluri N,
et al
.
total CD4 + T cells were isolated from murine spleens using
Biolegend
Mojo CD4
T cell
isolation kit (
480006
) and labelled with Cell Proliferation Dye (
eBioscience
, #
65-0842-85
) by incubating in a
1:2000
dilution
(8)
Targeting HK3 in tumor-associated macrophages enhances antitumor immunity through augmenting antigen cross-presentation in cervical cancer
J Immunother Cancer
July 15, 2025
Yifan Yang, Xun Tian, Jinglan Chen, Jian Liu, Han Jiang, Liting Liu, Shen Qu, Min Wu, Jingyu Wang, Ying Chen,
et al.
assay
Mouse
naïve CD8+ T cells and CD4+ T cells from
spleen
were extracted with an isolation kit (
480044
or
480005
,
BioLegend
). BMDMs were pulsed with ovalbumin (
OVA
) (100 µg/mL, vac-stova, InvivoGen) overnight in advance.
(9)
The OGT–TFF2 axis mediates intrahepatic crosstalk and MASH pathogenesis
Hepatology
June 30, 2025
Lei Zhang, Cuijuan Han, Man Mohan Shrestha, Jiamei Le, Wilhelm K. Berger, Yiheng Huang, Reina Desrouleaux, Eric Wang, Laszlo Nagy, Xiaoyong Yang
SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads
),
Cell Signaling Technology
,
9003S
MojoSort™ Mouse CD4 T Cell Isolation Kit
,
Biolegend
,
480006
MojoSort™ Mouse CD8 naïve T Cell Isolation Kit
,
Biolegend
,
480043
MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit
(10)
Hepatitis B virus promotes liver cancer by modulating the immune response to environmental carcinogens
Nature communications
June 27, 2025
Huang M, Wang D, Huang J, Bae AN, Xia Y, Zhao X, Mortaja M, Azin M, Collier MR, Semenov YR,
et al
.
CD4+ T cells were isolated using MojoSort mouse CD4+ T cells isolation kits (
Biolegend
, catalog no.
480006
). After
cell counting
, 5 × 106 cells were resuspended in 1 mL of R10 media containing 2 μg/mL of CD28
(11)
Fibroblast lipid metabolism through ACSL4 regulates epithelial sensitivity to ferroptosis in IBD
Nature Metabolism
June 26, 2025
Wesley Huang, Yuezhong Zhang, Nupur K. Das, Sumeet Solanki, Chesta Jain, Marwa O. El-Derany, Imhoi Koo, Hannah N. Bell, Noora Aabed, Rashi Singhal,
et al.
C57BL/6
mice
were isolated from the
spleen
using a
MojoSort mouse CD4 T Cell Isolation Kit
(
BioLegend
,
480005
) and sorted by
flow cytometry
using a
1:100
dilution of an anti-mouse CD4 PE-Cy7 antibody (
Invitrogen
,
(12)
A DNA Vaccine Against Proadrenomedullin N-Terminal 20 Peptide (PAMP) Reduces Angiogenesis and Increases Lymphocyte and Macrophage Infiltration but Has No Effect on Tumor Burden in a Mouse Model of Lung Metastasis
Vaccines (Basel)
May 30, 2025
Tom Kalathil Raju, Srdan Tadic, Pablo Garrido, Laura Ochoa-Callejero, Judit Narro-Íñiguez, Josune García-Sanmartín, Alfredo Martínez
1.0 × 106 cells/mL. Then, CD4+
lymphocytes
were purified with the MojoSort CD4
T Cell
Isolation Kit (
480005
,
BioLegend
) following the manufacturer’s instructions. The isolated
T cells
were kept in RPMI supplemented
(13)
Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunity
Sci Adv
April 18, 2025
Keisuke Imabayashi, Yutaro Yada, Kazuhiko Kawata, Motoki Yoshimura, Takeshi Iwasaki, Akemi Baba, Akihito Harada, Koichi Akashi, Hiroaki Niiro, Yoshihiro Baba
from bm12
mice
by negative selection using the
MojoSort Mouse CD4 T Cell Isolation Kit
(
BioLegend
,
480005
), were transferred into the recipient
mice
. On days 13 and 14 after B cell transfer,
diphtheria
toxin
(14)
CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells
Elife
April 4, 2025
Xiaoyu Meng, Yezhang Zhu, Kuai Liu, Yuxi Wang, Xiaoqian Liu, Chenxin Liu, Yan Zeng, Shuai Wang, Xianzhi Gao, Xin Shen,
et al.
Foxp3YFP-Cre and Foxp3YFP-CreCxxc1fl/fl
mice
were enriched using the Mouse CD4 T Cell Isolation Kit (
480005
;
BioLegend
) and then sorted using the BD Aria II
flow cytometer
. Naive CD4+T cells from 2D2 (MOG35-55-specific
(15)
Antrodia cinnamomea Formula Suppresses Prostate Cancer Progression via Immune Modulation and PD-1/PD-L1 Pathway Inhibition
Int J Mol Sci
March 17, 2025
Ming-Yen Tsai, Chung-Kuang Lu, Li-Hsin Shu, Hung-Te Liu, Yu-Huei Wu, Yu-Shih Lin, Yao-Hsu Yang, Wei-Tai Shih, I-Yun Lee, Yu-Heng Wu,
et al.
cocktails from the
MojoSort™ Mouse CD4+ T-Cell Isolation Kit
(
BioLegend
, San Diego, CA, USA, Catalog No.
480006
) or CD8+
T-Cell
Isolation Kit (
BioLegend
, San Diego, CA, USA, Catalog No.
480008
) were added to the
(16)
Antigen-presenting cancer associated fibroblasts enhance antitumor immunity and predict immunotherapy response
Nat Commun
March 4, 2025
Junquan Song, Rongyuan Wei, Chenchen Liu, Zhenxiong Zhao, Xuanjun Liu, Yanong Wang, Fenglin Liu, Xiaowen Liu
mice
. Filter and wash the suspension, then use mouse CD4+ T cells isolation kit (
Biolegend
; cat.no.
480006
) to sort the CD4+ T cells from the suspension. Wash the sorted CD4+ T cells and seed them into the 96-well
(17)
Lanatoside C activates the E3 ligase STUB1 to inhibit FOXP3 transcriptional activity and promote antitumor immunity
EMBO Molecular Medicine
February 20, 2025
Qian Zhou, Tong Yang, Xixi Yu, Bo Li, Jin Liu, Yongxin Mao, Rongxiang Guo, Zhuo Feng, Li Zhou, Guandi Zeng,
et al.
regulatory T cell
isolation kit,
Miltenyi
,
130-091-041
MojoSort mouse CD4+ T cell isolation kit
,
Biolegend
,
480005
Annexin V-FITC/PI Apoptosis Detection Kit
,
Vazyme
,
A211-02
Mouse IL10 Valukine ELISA Kit,R&D Systems,VAL605 Cytofix/Cytoperm
(18)
The effect and mechanism of astragalus polysaccharides on T cells and macrophages in inhibiting prostate cancer
Biomedical journal
February 1, 2025
Wu CY, Yang YH, Lin YS, Shu LH, Liu HT, Lu CK, Wu YH, Wu YH.
from the
MojoSort™ Mouse CD4 T Cell Isolation Kit
(
BioLegend Inc
, San Diego, CA, USA, catalog number:
480006
) or
MojoSort™ Mouse CD8 T Cell Isolation Kit
(
BioLegend Inc
, San Diego, CA, USA, catalog number:
480008
).
(19)
Glioblastoma induces the recruitment and differentiation of dendritic-like “hybrid” neutrophils from skull bone marrow
Cancer Cell
September 9, 2024
Meeki Lad, Angad S. Beniwal, Saket Jain, Poojan Shukla, Venina Kalistratova, Jangham Jung, Sumedh S. Shah, Garima Yagnik, Atul Saha, Ankita Sati,
et al.
Staining Buffer Set,
Thermo Fisher
,Cat#
00-5523-00
MojoSort™ Mouse CD4 T Cell Isolation Kit
,
Biolegend
,Cat#
480005
MojoSort™ Mouse Neutrophil Isolation Kit
,
Biolegend
,Cat#
480057
Mouse
Tumor
360 Profiling Codeset +
(20)
Investigation of immunomodulatory and cytotoxic effects of shed snake skin (Elaphe sauromates) extract
Frontiers in Pharmacology
July 12, 2024
Cagri Caglar Sinmez, Emre Tüfekçi, Büşra Şeniz Demir, Ahmet Eken, Vehbi Guneş, Seda Ekici, Esra Bozkaya, Ali İlteriş Aykun
were purified for some experiments using CD4 microbeads (
MojoSort™ Mouse CD4 T Cell Isolation Kit
, #
480006
,
BioLegend
) at ∼95% purity. Sorted CD4+ T cells were used for apoptosis, cytokine, and Treg differentiation
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Reviews
Write a Review
(1)
1/31/2020
CD4 Enrichment of Cultured Splenocytes Using MojoSort
Troy Trevino
The MojoSort Mouse CD4 T cell isolation kit works very well to enrich for CD4 cells in a primary splenocyte culture. When used in tandem with a dead cell separation medium like Lympholyte-M, high ...
Read Review
(1)
12/27/2019
Pan CD4 T Cell Isolation Kit from Mouse
Wei Xu
You can use this kit to get acceptable yield and purity to isolate CD4 cells from mouse with reasonable price.
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MojoSort™ Mouse CD4 T Cell Isolation Kit from BioLegend
Supplier Page
View Company Product Page
$483.00
Product Specs
Company Info
Citations
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