LDH-Cytotoxicity Assay Kit (Fluorometric) ab197004 from Abcam

Product Specs
ItemLDH-Cytotoxicity Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab197004
Quantity500 Test
Sample TypeAdherent cells, Suspension cells
SensitivityEqual to 100 Cells/well
Assay Time0h 20m
ApplicationsEnzyme activity assay
Gene NameldhA
NCBI Gene AliasesGSD11, HEL-S-133P, LDHM, PIG19
Research AreaMetabolism
NCBI Full Gene Namelactate dehydrogenase A

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) From Stress to Survival: Trophoblast-Derived Extracellular Vesicle Proteome Captures Aspirin-Driven Cellular Reprogramming in a Preeclampsia ModelPharmaceuticsMay 29, 2026Vineet Mahajan, Awanit Kumar, Jeena Jacob, Maged M. Costantine, Lauren S. Richardson, Rheanna Urrabaz-Garza, Emmanuel Amabebe, Ourlad Alzeus G. Tantengco, Ananth Kumar Kammala, Ramkumar MenonCytotoxicity and Viability Aspirin cytotoxicity (0–100 μg/mL) was assessed using LDH release (Abcam ab197004). Ten microliters of media were mixed with 90 μL LDH reaction mix, incubated for 10 min, and fluorescence

(2) KLF2 alleviates sepsis-induced acute kidney injury via the lncRNA GAS6-AS2/GOLPH3 axisBMC NephrologyApril 20, 2026Wei Feng, Yu-Feng Zhu, Kai-Yuan Li, Xiao-Shuang Xu, Bo-Yu Zhang, Jian Zhou(MDA) and glutathione (GSH) detection LDH activity in HK2 cells was determined by LDH detection kit (ab197004, Abcam) following the instructions of the manufacturer. ROS and MDA levels were measured by utilizing

(3) P-glycoprotein exofection between fetal and maternal cells as a mechanism of intercellular material transfer at the feto maternal interfacebioRxivJanuary 6, 2026Madhuri Tatiparthy, Amanda Wang, Vineeth Mahajan, Pilar Flores-Espinosa, Emmanuel Amabebe, Tilu Jain Thomas, Xiao-Ming Wang, Lauren S Richardson, Ramkumar Menon, Ananth K KammalaAccording to the manufacturer’s protocol, 5 μL of each supernatant was mixed with 95 μL of LDH reagent (ab197004, Abcam, Cambridge, UK) in a 96-well plate and incubated in the dark at room temperature for 10 minutes.

(4) Characteristics and Potential of KSL, KSL-W, and Dadapin-1 Antimicrobial Peptides for Preventing Infections of Orthopedic Prosthetic Devices: Identifying the Most Robust CandidateInt J Mol SciAugust 11, 2025Davide Campoccia, Andrea De Donno, Giulia Bottau, Gloria Bua, Stefano Ravaioli, Eleonora Capponi, Giovanna Sotgiu, Francesco Pegreffi, Silvia Costantini, Carla Renata Arciolameasuring the release of lactate dehydrogenase (LDH) from damaged cells by the LDH Cytotoxicity Assay (ab197004, Abcam, Cambridge, UK), strictly following the instructions of the manufacturer. For all treatments,

(5) Exosomal Delivery of Interleukin‐10 Reduces Infection‐Associated Inflammation in a 3D ‐Printed Model of a Humanized Feto–Maternal InterfaceThe FASEB JournalMay 31, 2025Leah M. Saylor, Rahul Cherukuri, Ananth K. Kammala, Lauren Richardson, Marc Ferrer, Cristina Antich, Shayne Frebert, Arum Han, Ramkumar Menonmetabolic activity analysis Lactate dehydrogenase (LDH) assays An LDH cytotoxicity detection kit (ab197004, Abcam, Cambridge, UK) was used to assess the cytotoxicity of cells cultivated within the 3D-printed

(6) Exosomal Delivery of Interleukin-10 Reduces Infection-Associated Inflammation in a 3D-Printed Model of a Humanized Feto–Maternal InterfaceFASEB JMay 31, 2025Leah M Saylor, Rahul Cherukuri, Ananth K Kammala, Lauren Richardson, Marc Ferrer, Cristina Antich, Shayne Frebert, Arum Han, Ramkumar Menonmetabolic activity analysis Lactate dehydrogenase (LDH) assays An LDH cytotoxicity detection kit (ab197004, Abcam, Cambridge, UK) was used to assess the cytotoxicity of cells cultivated within the 3D-printed

(7) A dynamic flow fetal membrane organ-on-a-chip system for modeling the effects of amniotic fluid motionBiomedical MicrodevicesSeptember 1, 2024Sungjin Kim, Po Yi Lam, Lauren S. Richardson, Ramkumar Menon, Arum Hanflow culture on the amnion epithelial cells cultured in the AM-OOC, a LDH cytotoxicity detection kit (ab197004, Abcam, Cambridge, UK) was used. Cell culture media from the cell culture chamber and reservoir in the

(8) A high throughput 3D-printed model of the feto-maternal interface for the discovery and development of preterm birth therapiesACS Applied Materials & InterfacesAugust 14, 2024Rahul Cherukuri, Ananth Kumar Kammala, Tilu Jain Thomas, Leah Saylor, Lauren Richardson, Sungjin Kim, Marc Ferrer, Cristina Acedo, Min Jae Song, Akhilesh K. Gaharwar, et al.cultivated within the 3D-printed devices, a lactate dehydrogenase (LDH) cytotoxicity detection kit (ab197004, Abcam, Cambridge, UK) was used. The LDH assay quantifies cellular cytotoxicity by measuring the enzymatic

(9) A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesiclesNature CommunicationsSeptember 11, 2023Kangfu Chen, Bill T. V. Duong, Sharif U. Ahmed, Piriththiv Dhavarasa, Zongjie Wang, Mahmoud Labib, Connor Flynn, Jingya Xu, Yi Y. Zhang, Hansen Wang, et al.cytotoxicity, we co-cultured sEV-treated CD8+ T cells with tumor cells and performed LDH-cytotoxicity assay (ab197004, Abcam). Briefly, sEVs from H1975 WT and MC38 WT were sorted through the NanoEPIC device and subsequently

(10) Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF-κB/NLRP3 AxisJournal of diabetes researchJanuary 1, 2023He J, Cui J, Shi Y, Wang T, Xin J, Li Y, Shan X, Zhu Z, Gao Y.concentrations in the cultured podocyte supernatant were measured via an LDH cytotoxicity assay kit (ab197004; Abcam). After the treatments, the supernatant was collected and centrifuged and then was plated in

(11) Dexmedetomidine pretreatment alleviates ropivacaine-induced neurotoxicity via the miR-10b-5p/BDNF axisBMC AnesthesiologySeptember 26, 2022Weicai Xu, Xiaojun Li, Long Chen, Xiaopan Luo, Sheliang Shen, Jing Wangafter 10 min of centrifugation. LDH release in the supernatant was detected using LDH cytotoxicity kit (ab197004, Abcam Inc., Cambridge, MA, USA) to determine the damage degree of HT22 cells or SH-SY5Y cells. Apoptosis detection

(12) TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cellsImmunity, inflammation and diseaseJuly 1, 2022Sun Y, Chen X, Xie Y, Wang Y, Zhang Q, Lu Y, Li X.peroxidase (GSH‐Px) activity, and malondialdehyde (MDA) level in RTECs were measured using the LDH (ab197004; Abcam), SOD (S0101), GSH‐Px (S0058), and MDA (S0131, Beyotime) assay kit, respectively, according to

(13) nurP28, a New-to-Nature Zein-Derived Peptide, Enhances the Therapeutic Effect of Docetaxel in Breast Cancer Monolayers and SpheroidsMoleculesApril 29, 2022Plinio Alejandro Trinidad-Calderón, Laura Margarita López-Castillo, Salvador Gallegos-Martínez, Grissel Trujillo-de Santiago, Silverio García-Lara, Mario Moisés ÁlvarezDehydrogenase Cytotoxicity Assay We performed the fluorometric LDH-cytotoxicity assay (Abcam, Boston, MA, USA ab197004) to assess the degree of cell death on spheroids in response to the exposure to our treatments. We collected

(14) Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsisExperimental and Therapeutic MedicineOctober 1, 2021Zhongyuan Zhang, Meifeng Liang, Xiongfei Wanintestinal fatty acid-binding protein (iFABP). The assays were conducted using an LDH Assay kit (cat. no. ab197004; Abcam), DAO Activity Assay kit (cat. no. ab273325; Abcam) and iFABP ELISA kit [cat. no. JK-(a)-5035;

(15) Cadmium exposure activates Akt/ERK Signaling and pro-inflammatory COX-2 expression in human gallbladder epithelial cells via a ROS dependent mechanismToxicology in vitro : an international journal published in association with BIBRASeptember 1, 2020Sharma P, Caldwell TS, Rivera MN, Gullapalli RR.instructions. 4) Lactate dehydrogenase cytotoxicity assay: The fluorometric LDH-cytotoxicity assay (cat # ab197004, Abcam, CA, USA) was performed to quantify the degree of cell death resulting from Cd exposures. In

(16) Tetrahydroxystilbene glucoside protects against LPS-induced endothelial dysfunction via inhibiting RhoA/ROCK signaling and F-actin remodelingGeneral physiology and biophysicsMarch 16, 2020Yan Qi, Xiuying Liang, Xia Hu, Haiyan He, Lian Tang, Wenjuan Yaokit was purchased from Clontech (Mountain View, CA, USA). The LDH (lactate dehydrogenase) assay kit (ab197004) was obtained from Abcam (Cambridge, MA, USA). The specific ROCK inhibitor Y-27632 was purchased from

(17) Does Maternal Stress Affect the Early Embryonic Microenvironment? Impact of Long-Term Cortisol Stimulation on the Oviduct EpitheliumInternational Journal of Molecular SciencesJanuary 10, 2020Shuaizhi Du, Nares Trakooljul, Jennifer Schoen, Shuai ChenAssays, Ann Arbor, MI, USA; K017-C1). The fluorometric LDH activity assay kit (Abcam, Boston, MA, USA; ab197004) was used to determine cellular cytotoxicity. For all assays, samples were measured in duplicate. A

(18) Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the EndotheliumCell reportsJanuary 1, 0001"Oyama Y, Bartman CM, Bonney S, Lee JS, Walker LA, Han J, Borchers CH, Buttrick PM, Aherne CM, Clendenen N, et al."iQ SYBR Green Supermix,Bio-rad,Cat# 1708880 L-Lactate Assay Kit,Abcam,Ca# ab65331 LDH-Cytotoxicity Assay Kit,Abcam,Ca# ab197004 ChIP-IT Express Chromatin Immunoprecipitation Kits,Active Motif,Ca# 53009 Pierce Co-Immunoprecipitation (Co-IP

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LDH-Cytotoxicity Assay Kit (Fluorometric) from Abcam

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LDH-Cytotoxicity Assay Kit (Fluorometric) from Abcam

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