Maxwell RSC miRNA Tissue Kit AS1460 from Promega

Product Specs
ItemMaxwell RSC miRNA Tissue Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1460
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA, Small RNA, miRNA
Sample TypeTissue samples

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) A MicroRNA Next-Generation-Sequencing Discovery Assay (miND) for Genome-Scale Analysis and Absolute Quantitation of Circulating MicroRNA BiomarkersInternational Journal of Molecular SciencesJanuary 22, 2022Kseniya Khamina et al.extracted from 200 μL of plasma samples with Maxwell RSC miRNA Tissue kit (Promega, Madison, WI, USA, AS1460) according to the manufacturer’s protocol. Briefly, samples were thawed on ice and centrifuged at 12,000×

(42) Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virusNature BiotechnologyJanuary 1, 2022Do Yon Kim, Jeong Mi Lee, Su Bin Moon, Hyun Jung Chin, Seyeon Park, Youjung Lim, Daesik Kim, Taeyoung Koo, Jeong-Heon Ko, Yong-Sam Kimof transfection. Total RNA was prepared using a Maxwell RSC miRNA Tissue Kit (Promega, catalog no. AS1460). RNA was poly(adenylated) by incubating 5 μg of an RNA preparation with E. coli poly(A) polymerase

(43) miR-199a Downregulation as a Driver of the NOX4/HIF-1α/VEGF-A Pathway in Thyroid and Orbital Adipose Tissues from Graves' PatientsInternational journal of molecular sciencesDecember 23, 2021Craps J, Joris V, Baldeschi L, Daumerie C, Camboni A, Buemi A, Lengelé B, Behets C, Boschi A, Mourad M, et al.15 min at 37 °C. Extractions were performed with the Maxwell® RSC miRNA Tissue Kit (Promega, Cat # AS1460) or the Maxwell® RSC miRNA plasma and serum kit (Promega, Cat # AS1680). Maxwell® RSC Cartridges were

(44) Differential Effects of Insulin-Deficient Diabetes Mellitus on Visceral vs. Subcutaneous Adipose Tissue—Multi-omics Insights From the Munich MIDY Pig ModelFrontiers in MedicineNovember 23, 2021Florian Flenkenthaler et al.

(45) Vascular Tissue Specific miRNA Profiles Reveal Novel Correlations with Risk Factors in Coronary Artery DiseaseBiomoleculesNovember 12, 2021Katrīna D. Neiburga et al.IMA samples were used for further semi-automated miR extraction using Maxwell RSC miRNA Tissue Kit (AS1460) based on a Maxwell RSC 48 system (Promega) at the German Heart Centre Munich. After removal of surgical

(46) MicroRNA Expression in Extracellular Vesicles from Nasal Lavage Fluid in Chronic RhinosinusitisBiomedicinesApril 26, 2021Seungbin Cha, Eun-Hye Seo, Seung Hyun Lee, Kyung Soo Kim, Chung-Sik Oh, Jong-Seok Moon, Jin Kook Kimfrom EVs Total RNA was extracted from 400 µL of EV using the Promega Maxwell® RSC miRNA Tissue Kit (AS1460; Promega Corporation, Fitchburg, WI, USA) and Maxwell® RSC instrument (Promega Corporation, Madison,

(47) A First Phenotypic and Functional Characterization of Placental Extracellular Vesicles from Women with Multiple SclerosisInternational journal of molecular sciencesMarch 12, 2021Martire S, Montarolo F, Spadaro M, Perga S, Sforza ML, Marozio L, Frezet F, Bruno S, Chiabotto G, Deregibus MC, et al.the Maxwell® RSC Instrument (Promega, Madison, Wisconsin, USA) with Maxwell® RSC miRNA Tissue Kit (AS1460, Promega, Madison, Wisconsin, USA) according to manufacturer’s protocol, and stored at −80 ◦ C. RNA

(48) Comparison and optimisation of microRNA extraction from the plasma of healthy pregnant womenMolecular Medicine ReportsFebruary 5, 2021Victoria L. Parker, Bryony F. Cushen, Eleftherios Gavriil, Benjamin Marshall, Sarah Waite, Allan Pacey, Paul R. Heathhttps://www.promega. co.uk/products/nucleic-acid-extraction/rna/maxwell-rscmirnatissue-plasma-serum-kit/?catNum=AS1460#protocols. 45. Promega: Maxwell® RSC Instrument, AS4500. https://www. promega.co.uk/products/lab-automation/maxwell-instruments/ maxwell-rsc-instrument/?catNum=AS4500 46.

(49) Chronic Kidney Disease-Associated Inflammation Increases the Risks of Acute Kidney Injury and Mortality after Cardiac SurgeryInternational Journal of Molecular SciencesDecember 18, 2020Angela Casas et al.Isolation Tubes, StemCell technologies, Vancouver, BC, Canada), Maxwell RSC miRNA Tissue Kit (Cat. # AS1460, Promega, Madison, WI, USA), Maxwell RSC miRNA Plasma and Serum Kit (Cat. # AS1680, Promega, Madison,

(50) Profiling microRNAs in uncomplicated pregnancies: Serum vs. plasmaBiomedical ReportsDecember 14, 2020Victoria L Parker, Eleftherios Gavriil, Benjamin Marshall, Allan Pacey, Paul R Heathversion of the Maxwell® Rapid Sample Concentrator (RSC) miRNA Tissue or Plasma Serum kit (cat. no. AS1460; Promega Corporation) (57) with minor modifications to the standard protocol. Specifically, lyophilised

(51) The Association of Residential Altitude on the Molecular Profile and Survival of Melanoma: Results of an Interreg StudyCancers (Basel)September 29, 2020Eleonora De Martino et al.with DNase I. The subsequent steps follow the Maxwell® RSC miRNA Tissue Kit instructions (Cat. No. AS1460, Promega, Madison, WI 53711-5399, USA). The 1-Thioglycerol/Homogenization solution and the Lysis Buffer

(52) Pazopanib and Trametinib as a Synergistic Strategy against Osteosarcoma: Preclinical Activity and Molecular InsightsCancers (Basel)June 10, 2020Giulia Chiabotto et al.in combination, and then were subjected to RNA extraction, using the Maxwell® RSC miRNA Tissue Kit AS1460 (Promega). Namely, 106 cells were lysed with 1-Thioglycerol/Homogenization Solution. RNA samples were

(53) Dysfunctional polycomb transcriptional repression contributes to lamin A/C–dependent muscular dystrophyJournal of Clinical InvestigationApril 6, 2020Andrea Bianchi et al.analysis Total RNA was extracted from satellite cells using a Maxwell RSC miRNA tissue kit (Promega, AS1460), while total RNA from muscle tissues was extracted using TRI Reagent (MilliporeSigma, T9424). cDNA

(54) Integrated miRNA/mRNA Counter-Expression Analysis Highlights Oxidative Stress-Related Genes CCR7 and FOXO1 as Blood Markers of Coronary Arterial DiseaseInternational Journal of Molecular SciencesMarch 12, 2020Miguel Hueso, Adrián Mallén, Ángela Casas, Jordi Guiteras, Fabrizio Sbraga, Arnau Blasco-Lucas, Núria Lloberas, Joan Torras, Josep M Cruzado, Estanislao NavarroIsolation Tubes, StemCell technologies, Vancouver, BC, Canada), Maxwell RSC miRNA Tissue Kit (Cat.# AS1460, Promega, Madison, WI, USA), TaqMan® Advanced miRNA Assays–single tube assays (Cat.#A25576, Thermo Fisher,

(55) A pathway coordinated by DELE1 relays mitochondrial stress to the cytosolNatureMarch 1, 2020Fessler E, Eckl EM, Schmitt S, Mancilla IA, Meyer-Bender MF, Hanf M, Philippou-Massier J, Krebs S, Zischka H, Jae LT.at −80 °C. RNA of 300 μl TRIzol lysate was isolated using the Maxwell RSC miRNA Tissue Kit (Promega, AS1460) following the manufacturer’s instructions. Afterwards, the RNA concentration was quantified (NanoDrop,

(56) Reliability of miRNA Analysis from Fixed and Paraffin-Embedded TissuesInternational Journal of Molecular SciencesSeptember 27, 2019Eros Azzalini, Eleonora De Martino, Paolo Fattorini, Vincenzo Canzonieri, Giorgio Stanta, Serena Bonininto the cartridge of the Maxwell® RSC miRNA tissue kit (Promega, Madison, WI 53711-5399, USA; code AS1460) to allow miRNA, as well as total RNA, isolation following the manufacturer’s procedures. Elution of

(57) Evolving neoantigen profiles in colorectal cancers with DNA repair defectsGenome medicineJune 28, 2019Rospo G, Lorenzato A, Amirouchene-Angelozzi N, Magrì A, Cancelliere C, Corti G, Negrino C, Amodio V, Montone M, Bartolini A, et al.analysis Total RNA was extracted from a pellet of CRC cells (2 × 106 cells) using Maxwell® RSC miRNA Tissue Kit (AS1460, Promega), according to the manufacturer’s protocol. The quantification of RNA was performed by Thermo

(58) MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the EndotheliumArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2018Virginie Joris, Elvira Leon Gomez, Lisa Menchi, Irina Lobysheva, Vittoria Di Mauro, Hrag Esfahani, Gianluigi Condorelli, Jean-Luc Balligand, Daniele Catalucci, Chantal Dessywere suspended in homogenisation buffer provided by Promega in the Maxwell RSC miRNA Tissue Kit (ref AS1460) and added with thioglycerol (20 µl/1 mL). Heart and vessel tissues were crushed in homogenisation buffer

(59) Circular RNA CpG island hypermethylation-associated silencing in human cancerOncotargetJune 26, 2018Ferreira HJ, Davalos V, de Moura MC, Soler M, Perez-Salvia M, Bueno-Costa A, Setien F, Moran S, Villanueva A, Esteller M.colon cancer cell lines HCT116 and DKO were purified using the Maxwell® RSC miRNA Tissue Kit (Cat. #AS1460, Promega) in the automated Maxwell® RSC Instrument (Promega), according to the manufacturer’s instructions.

(60) microRNA expression in tumour tissue and plasma in patients with newly diagnosed metastatic prostate cancerTumor BiologyMay 19, 2018Ahmed Hussein Zedan, Torben Frøstrup Hansen, Jannie Assenholt, Mindaugas Pleckaitis, Jonna Skov Madsen, Palle Jörn Sloth Osthersamples by the addition of 180 mL incubation buffer (Promega, AS1135) and 20 mL Proteinase K (Promega, AS1460) and incubated for 1 h at 70°C. And 400 mL lysis buffer (Promega, AS1460) was added and the reaction

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Maxwell RSC miRNA Tissue Kit

Maxwell RSC miRNA Tissue Kit from Promega

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Maxwell RSC miRNA Tissue Kit from Promega

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