Maxwell RSC miRNA Tissue Kit AS1460 from Promega

Product Specs
ItemMaxwell RSC miRNA Tissue Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1460
Quantity48 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA, Small RNA, miRNA
Sample TypeTissue samples

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) MicroRNA expression signature as a biomarker in the diagnosis of nodal T-cell lymphomasCancer Cell InternationalJanuary 30, 2024Muhammad Sufyan Bin Masroni, Gracie Wee Ling Eng, Ah-Jung Jeon, Yuan Gao, He Cheng, Sai Mun Leong, Jit Kong Cheong, Susan Swee-Shan Hue, Soo Yong Tantransferred to a fresh tube and subjected to RNA isolation using the Maxwell RSC miRNA Tissue kit (Promega, AS1460) and the Maxwell RSC system (Promega) in accordance with the manufacturer’s recommendation. A proprietary

(22) Non-canonical antigens are the largest fraction of peptides presented by MHC class I in mismatch repair deficient murine colorectal cancerGenome MedicineJanuary 19, 2024Giuseppe Rospo, Rosaria Chilà, Vittoria Matafora, Veronica Basso, Simona Lamba, Alice Bartolini, Angela Bachi, Federica Di Nicolantonio, Anna Mondino, Giovanni Germano, et al.analysis Total RNA was extracted from CT26 Mlh1+/+ and CT26 Mlh1-/- cells using Maxwell® RSC miRNA Tissue Kit (AS1460, Promega), according to the manufacturer’s protocol. The quantification of RNA was performed by DeNovix

(23) Protocol for in vitro establishment of heterogeneous stem-like cultures derived from whole human glioblastoma tumorsSTAR ProtocolsDecember 1, 2023Francesca De Bacco, Francesca Orzan, Elena Casanova, Marta Prelli, Carla BoccaccioPCR-based",Voden,cat#11-1250 ReliaPrep gDNA Tissue Miniprep System,Promega,cat#A2050 Maxwell RSC miRNA Tissue Kit,Promega,cat#AS1460 PureLink Genomic DNA Mini Kit,Invitrogen,cat#K1820-00 ,, Software and algorithms,Software and algorithms,Software

(24) HHV-6A Infection of Papillary Thyroid Cancer Cells Induces Several Effects Related to Cancer ProgressionVirusesOctober 19, 2023Mardente S, Romeo MA, Asquino A, Po A, Gilardini Montani MS, Cirone M.by an automated Maxwell RSC-Promega extractor, using the Maxwell RSC miRNA Tissue Kit miRNA (CAT # AS1460, Promega, Sydney, Australia). Retro-transcription was performed using a Taq-Man MicroRNA Reverse Transcription

(25) Targeting FGFRs by pemigatinib induces G1 phase cell cycle arrest, cellular stress and upregulation of tumor suppressor microRNAsJournal of translational medicineSeptember 15, 2023Pace A, Scirocchi F, Napoletano C, Zizzari IG, Po A, Megiorni F, Asquino A, Pontecorvi P, Rahimi H, Marchese C, et al.obtained by an automated Maxwell RSC-Promega extractor using the Maxwell RSC miRNA Tissue Kit (CAT # AS1460, Promega). For microRNA (miRNA) expression analysis, a miRNA-specific RT was carried out using Taq-Man™

(26) Virus Inactivation by Formaldehyde and Common Lysis BuffersVirusesAugust 4, 2023Ulrike Seeburg, Lorena Urda, Fabian Otte, Martin J. Lett, Silvia Caimi, Christian Mittelholzer, Thomas Klimkaitsupernatant A671,Promega,AS1620—Cultured Cells DNA Kit,Guanidinium thiocyanate,infected cells MC136A,Promega,AS1460—Maxwell® RSC miRNA from Tissue and Plasma/Serum Kit,Guanidine thiocyanate1-thioglycerol,viral cell culture

(27) Targeting lysine-specific demethylase 1 (KDM1A/LSD1) impairs colorectal cancer tumorigenesis by affecting cancer cells stemness, motility, and differentiationCell death discoveryJune 29, 2023Antona A, Leo G, Favero F, Varalda M, Venetucci J, Faletti S, Todaro M, Mazzucco E, Soligo E, Saglietti C, et al.miRNA extraction, 500,000 cells/condition were processed with Maxwell® RSC miRNA Tissue KIT (code: AS1460; Promega, Madison, WI, USA) according to the manufacturer’s instructions. RNA samples were quantified

(28) Evaluation of a Set of miRNAs in 26 Cases of Fatal Traumatic Brain InjuriesInternational Journal of Molecular SciencesJune 29, 2023Serena Bonin, Stefano D’Errico, Caterina Medeot, Carlo Moreschi, Solange Sorçaburu Ciglieri, Michela Peruch, Monica Concato, Eros Azzalini, Carlo Previderè, Paolo Fattoriniwith DNase I. The subsequent steps follow the Maxwell® RSC miRNA Tissue Kit instructions (Cat. No. AS1460, Promega, Madison, WI, USA). The 1-Thioglycerol Homogenization solution and the Lysis Buffer (MC501C)

(29) Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoidseLifeJune 1, 2023Silver B, Gerrish K, Tokar E.in culture media. Nucleic acid extraction RNA and gDNA from organoids (Promega, miRNA Tissue Kit AS1460; Tissue DNA Kit AS1610) and cfDNA from media (Promega, ccfDNA Plasma Kit AS1480) were extracted using

(30) Dual VEGFA/BRAF targeting boosts PD‐1 blockade in melanoma through GM‐CSF‐mediated infiltration of M1 macrophagesMolecular OncologyMay 27, 2023Valentina Comunanza, Chiara Gigliotti, Simona Lamba, Gabriella Doronzo, Edoardo Vallariello, Valentina Martin, Claudio Isella, Enzo Medico, Alberto Bardelli, Dario Sangiolo, et al.CAACGTTCTCCGTTCTCTTGCT‐3′. Total RNA was extracted from tumors using Maxwell® RSC miRNA Tissue kit (AS1460; Promega, Madison, WI, USA). Reverse transcription (RT) and qRT‐PCR were performed as previously described

(31) Identification of Suitable Internal Control miRNAs in Bovine Milk Small Extracellular Vesicles for Normalization in Quantitative Real-Time Polymerase Chain ReactionMembranesFebruary 2, 2023Rahman MM, Nakanishi R, Tsukada F, Takashima S, Wakihara Y, Kamatari YO, Shimizu K, Okada A, Inoshima Y.the bovine milk sEVs using the miRNeasy Micro Kit (217084, Qiagen) and Maxwell RSC miRNA Tissue Kit (AS1460, Promega), respectively, according to the manufacturer’s instructions. Immediately following extraction,

(32) Extra-Cellular Vesicles Derived from Thyroid Cancer Cells Promote the Epithelial to Mesenchymal Transition (EMT) and the Transfer of Malignant Phenotypes through Immune Mediated MechanismsInternational journal of molecular sciencesFebruary 1, 2023Mardente S, Aventaggiato M, Splendiani E, Mari E, Zicari A, Catanzaro G, Po A, Coppola L, Tafani M.automated Maxwell RSC-Promega extractor, using, respectively, the Maxwell RSC miRNA Tissue Kit miRNA (CAT # AS1460, Promega) and Maxwell RSC miRNA Plasma and Serum kit (CAT N.AS1680, Promega). Retro-transcription for

(33) Global Impairment of Immediate-Early Genes Expression in Rett Syndrome Models and Patients Linked to Myelination DefectsInternational journal of molecular sciencesJanuary 11, 2023Petazzi P, Jorge-Torres OC, Gomez A, Scognamiglio I, Serra-Musach J, Merkel A, Grases D, Xiol C, O'Callaghan M, Armstrong J, et al.For RTT post-mortem human brain, the total RNA was extracted with the Maxwell RSC miRNA Tissue Kit (AS1460, Promega, Madison, WI, USA), eluted with RNase-free water and treated with turbo DNase (Ambion). RNA

(34) The NALCN channel regulates metastasis and nonmalignant cell disseminationNature geneticsDecember 1, 2022Rahrmann EP, Shorthouse D, Jassim A, Hu LP, Ortiz M, Mahler-Araujo B, Vogel P, Paez-Ribes M, Fatemi A, Hannon GJ, et al.RNA sequencing Total RNA was extracted from tissues using Maxwell RSC miRNA Tissue Kit (catalog number AS1460, Promega). RNA quality was assessed using TapeStation System (catalog number 5067-5579, Agilent). RNA

(35) miR-199a-3p increases the anti-tumor activity of palbociclib in liver cancer modelsMolecular Therapy - Nucleic AcidsSeptember 13, 2022Elisa Callegari, Paola Guerriero, Cristian Bassi, Lucilla Dâ€™Abundo, Antonio Frassoldati, Edi Simoni, Laura Astolfi, Enrico Maria Silini, Silvia Sabbioni, Massimo NegriniInstrument (Promega Corporation, Madison, WI, USA) with the purification kit Maxwell RSC miRNA from Tissue (#AS1460, Promega) according to the manufacturer’s instructions. Droplet digital polymerase chain reaction (ddPCR)

(36) Human-rat integrated microRNAs profiling identified a new neonatal cerebral hypoxic-ischemic pathway melatonin-sensitiveJournal of pineal researchSeptember 1, 2022Weiss MD, Carloni S, Vanzolini T, Coppari S, Balduini W, Buonocore G, Longini M, Perrone S, Sura L, Mohammadi A, et al.from the plasma by using an automated Maxwell RSC extractor and Maxwell® RSC miRNA Tissue Kit (Promega AS1460). Four hundred microliters of plasma were mixed with 200 μl of 1‐thioglycerol/homogenization solution;

(37) Circulating miR-1246 and miR-485-3p as Promising Biomarkers of Clinical Response and Outcome in Melanoma Patients Treated with Targeted TherapyCancers (Basel)July 29, 2022Lauretta Levati et al.including miRNAs, was isolated from 400 μL of plasma samples using the Maxwell® RSC miRNA Tissue Kit AS1460 (Promega, Madison, WI, USA) in combination with the Maxwell® RSC Instrument AS4500 (Promega). Plasma

(38) Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndromeMolecular Therapy - Nucleic AcidsMarch 8, 2022Edilene Siqueira et al.circRNA, and T-UCR expression analysis, total RNA was extracted with the Maxwell RSC miRNA Tissue Kit (AS1460, Promega, Madison, WI, USA). The isolated RNA was treated with RQ1 RNase-Free DNase (M6101, Promega,

(39) The transcribed ultraconserved region uc.160+ enhances processing and A‐to‐I editing of the miR‐376 cluster: hypermethylation improves glioma prognosisMolecular OncologyFebruary 1, 2022Marta Soler, Veronica Davalos, Anaís Sánchez‐Castillo, Carlos Mora‐Martinez, Fernando Setién, Edilene Siqueira, Manuel Castro de Moura, Manel Esteller, Sonia Guiland RT‐qPCR Total RNA, including miRNAs, was extracted with a Promega Maxwell® RSC miRNA Tissue kit (AS1460, Promega, Madison, WI, USA) according to the manufacturer's recommendations. For expression analysis,

(40) Association of cardiometabolic microRNAs with COVID-19 severity and mortalityCardiovascular ResearchJanuary 29, 2022Clemens Gutmann et al.RNA was extracted from 200 μL of citrate/CTAD plasma using the Maxwell RSC miRNA Tissue kit (Promega, AS1460) according to the manufacturer’s protocol. Briefly, samples were thawed on ice and centrifuged at 12

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Maxwell RSC miRNA Tissue Kit

Maxwell RSC miRNA Tissue Kit from Promega

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Maxwell RSC miRNA Tissue Kit from Promega

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