Description
The PowerQuant System is a 5-color, 4-target probe-based qPCR assay that simultaneously quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. During amplification, annealing of the probe to its target sequence generates a substrate that is cleaved by the 5' nuclease activity of Taq DNA polymerase when the enzyme extends from an upstream primer into the region of the probe. This liberates the fluorescent dye from its proximal position to the quencher and, therefore, an increase in amplification cycles leads to increase in fluorescence.