Maxwell RSC RNA FFPE Kit AS1440 from Promega

Product Specs
ItemMaxwell RSC RNA FFPE Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1440
Quantity48 preps
SourceCell/Tissue, Fixed Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFormalin-fixed, paraffin-embedded tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) Lung adenocarcinoma patients with malignant pleural effusions in hot adaptive immunity status have a longer overall survivalFrontiers in OncologyOctober 4, 2022Cheng-Guang Wu, Ruben Casanova, Fabian Mairinger, Alex Soltermannblocks (two sections, each 5 µm thick) using the Maxwell purification system (Maxwell RSC RNA FFPE Kit, AS1440, Promega). RNA was eluted in 50 µl RNase-free water and stored at -80°C. RNA concentration was measured

(42) Transcriptome-Wide Gene Expression Profiles from FFPE Materials Based on a Nuclease Protection Assay Reveals Significantly Different Patterns between Synovial Sarcomas and Morphologic MimickersCancers (Basel)September 28, 2022Sabrina Borchert, Thomas Herold, Stavros Kalbourtzis, Rainer Hamacher, Yvonne Krause, Sophia Berger, Wiebke K. Guder, Arne Streitbuerger, Jendrik Hardes, Moritz Goetz, et al.performed on 2–6 10 µm thick FFPE sections and RNA was isolated by using the Maxwell® RSC RNA FFPE Kit (AS1440, Promega, WI, USA), according to the manufacturer’s instructions. RNA was eluted in 50 µL RNase-free

(43) Improved Bladder Tumor RNA Isolation from Archived Tissues Using Methylene Blue for Normalization, Multiplex RNA Hybridization, Sequencing and SubtypingInternational Journal of Molecular SciencesSeptember 6, 2022Stefanie A. Köhler et al.RNAse-free DNase I (Roche, #04716728001) was added for 1 h at 37 °C. (2) Maxwell RSC RNA FFPE kit (Promega, #AS1440) (Method B). Samples were processed according to manufacturer’s instructions, starting at the Proteinase

(44) Setting Up an Ultra-Fast Next-Generation Sequencing Approach as Reflex Testing at Diagnosis of Non-Squamous Non-Small Cell Lung Cancer; Experience of a Single Center (LPCE, Nice, France)Cancers (Basel)April 30, 2022Marius Ilié et al.Maxwell RSC FFPE Plus DNA kit (catalog number AS1720) and Maxwell RSC RNA FFPE kit (catalog number AS1440), or using the Ion Torrent™Genexus™Purification System (Thermo Fisher Scientific, Waltham, MA, USA;

(45) Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental TissueVirusesMarch 14, 2022Marina Wierz, Beate Sauerbrei, Petra Wandernoth, Mark Kriegsmann, Rita Casadonte, Katharina Kriegsmann, Jörg Kriegsmannpurification of total RNA from FFPE tissue was performed using the Maxwell® RSC RNA FFPE Kit (Promega, ref. AS1440) following the manufacturer’s instructions. In brief, FFPE tissue sections were deparaffinized with

(46) CAF-Associated Paracrine Signaling Worsens Outcome and Potentially Contributes to Chemoresistance in Epithelial Ovarian CancerFrontiers in OncologyMarch 3, 2022Michael Wessolly, Elena Mairinger, Sabrina Borchert, Agnes Bankfalvi, Pawel Mach, Kurt Werner Schmid, Rainer Kimmig, Paul Buderath, Fabian Dominik Mairingerthe help of the Maxwell® RSC Instrument (Promega, Wisconsin, USA) using a Maxwell® RSC RNA FFPE kit (AS1440, Promega, Wisconsin, USA). The process was conducted according to the manufacturer’s instructions. In

(47) Clinical Value of NGS Genomic Studies for Clinical Management of Pediatric and Young Adult Bone SarcomasCancers (Basel)October 29, 2021Miriam Gutiérrez-Jimeno et al.Maxwell® RSC DNA FFPE kit (Promega, AS1450, Madison, WI, USA) for DNA and a Maxwell® RSC RNA FFPE kit (Promega, AS1440) for RNA. The amount of DNA and RNA was quantified using a Qubit fluorometer, and the amount used for

(48) Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissuePLOS ONESeptember 16, 2021Alireza Saraji et al.with a scalpel. RNA was isolated using the automatic bead-based Maxwell® RSC RNA FFPE Kit (Cat. No: AS1440, Promega). According to the manufacturer’s manual, the scraped tissue was transferred into a RNase-free

(49) Mitogen signal-associated pathways, energy metabolism regulation, and mediation of tumor immunogenicity play essential roles in the cellular response of malignant pleural mesotheliomas to platinum-based treatment: a retrospective studyTranslational Lung Cancer ResearchJuly 1, 2021Alexander Mathilakathu et al.isolation. RNA was isolated by a semi-automatic Maxwell purification system (Maxwell RSC RNA FFPE Kit, AS1440, Promega). Purification was performed according to the manufacturer’s instructions. RNA was eluted in

(50) Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone CystsArchives of Pathology & Laboratory MedicineJuly 1, 2021Josephine K. Dermawan et al.molecular testing. Total nucleic acid extraction was performed by using the Maxwell RSC FFPE Kits (catalog Nos. AS1440 and AS1450, Promega) and protocol on the Maxwell RSC instrument. Total nucleic acid was quantified by

(51) Immuno-priming durvalumab with bevacizumab in HER2-negative advanced breast cancer: a pilot clinical trialBreast Cancer ResearchDecember 1, 2020Miguel Quintela-Fandino et al.formalin-fixed paraffin-embedded tissue samples was isolated using the Maxwell® RSC RNA FFPE kit (#AS1440, Promega) with the Maxwell® RSC Instrument (Promega) according to the manufacturer’s protocol. Sequencing

(52) A Novel HPLC-Based Method to Investigate on RNA after FixationInternational Journal of Molecular SciencesOctober 13, 2020Paolo Fattorini, Cristina Forzato, Domenico Tierno, Eleonora De Martino, Eros Azzalini, Vincenzo Canzonieri, Giorgio Stanta, Serena Boninhomogenization was performed with 50 µl of Lysis solution obtained by the Maxwell® RSC RNA FFPE kit (Cat. No. AS1440; Promega, Madison, WI, USA) using a homogenizer (Ultra-Turrax T25 basic. IKA®-WERKE; Staufen, Germany).

(53) The Association of Residential Altitude on the Molecular Profile and Survival of Melanoma: Results of an Interreg StudyCancers (Basel)September 29, 2020Eleonora De Martino et al.protocol was subdivided into two parts: the first part refers to the Maxwell® RSC RNA FFPE kit (Cat. No. AS1440, Promega, Madison, WI 53711-5399, USA) where the sections were de-waxed, digested with proteinase K,

(54) Plasma Next Generation Sequencing and Droplet Digital-qPCR-Based Quantification of Circulating Cell-Free RNA for Noninvasive Early Detection of CancerCancers (Basel)February 4, 2020Martin Metzenmacher et al.FFPE Sections RNA was isolated from FFPE sections using the Maxwell® RSC RNA FFPE kit (Promega, cat# AS1440), following manufacturer’s instructions. Briefly, 2.0 mm3 of tissue sections were placed in a 1.5 mL

(55) Correlation of HPV16 Gene Status and Gene Expression With Antibody Seropositivity and TIL Status in OPSCCFrontiers in oncologyJanuary 1, 2020von Witzleben A, Currall E, Wood O, Chudley L, Akinyegun O, Thomas J, Bendjama K, Thomas GJ, Friedmann PS, King EV, et al.accordance to the manufacturer’s protocol (Maxwell® RSC DNA FFPE Kit, AS1450, Maxwell® RSC RNA FFPE Kit, AS1440, Promega, Southampton, UK). The concentrations of DNA and RNA were measured using the Nanodrop 2000

(56) Prognostic Immune Cell Profiling of Malignant Pleural Effusion Patients by Computerized Immunohistochemical and Transcriptional AnalysisCancers (Basel)December 5, 2019Chengguang Wu, Fabian Mairinger, Ruben Casanova, Aashil A. Batavia, Anne-Laure Leblond, Alex Soltermann(two cut sections, each 5 µm thick) using the Maxwell purification system (Maxwell RSC RNA FFPE Kit, AS1440, Promega). RNA was eluted in 50 µl RNase-free water and stored at −80 °C. RNA concentration was measured

(57) Digital Immune-Related Gene Expression Signatures In High-Grade Serous Ovarian Carcinoma: Developing Prediction Models For Platinum ResponseCancer Management and ResearchNovember 12, 2019Fabian Mairinger, Agnes Bankfalvi, Kurt Werner Schmid, Elena Mairinger, Pawel Mach, Robert FH Walter, Sabrina Borchert, Sabine Kasimir-Bauer, Rainer Kimmig, Paul Buderath16-Nov-2021 For personal use only. Mairinger et al puriﬁcation system (Maxwell RSC RNA FFPE Kit, AS1440, Promega, Wisconsin, USA). The puriﬁcation was performed according to the manufacturer’s instructions.

(58) Reliability of miRNA Analysis from Fixed and Paraffin-Embedded TissuesInternational Journal of Molecular SciencesSeptember 27, 2019Eros Azzalini, Eleonora De Martino, Paolo Fattorini, Vincenzo Canzonieri, Giorgio Stanta, Serena Boninmineral oil as provided by the Maxwell® RSC RNA FFPE kit (Promega, Madison, WI 53711-5399, USA; code AS1440). Procedures of the abovementioned kit were strictly followed for protein digestion by proteinase K

(59) RNA based individualized drug selection in breast cancer patients without patient-matched normal tissueOncotargetAugust 17, 2018Michael Forster et al.from the Avera Cancer Institute's FFPE samples was isolated using the Maxwell RSC RNA FFPE Kit (Cat # AS1440, Promega, Madison, WI, USA) on the Maxwell automated system (Cat # AS4500, Promega, Madison, WI, USA)

(60) Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type micePloS oneJanuary 1, 2018Ilievski V, Zuchowska PK, Green SJ, Toth PT, Ragozzino ME, Le K, Aljewari HW, O'Brien-Simpson NM, Reynolds EC, Watanabe K.using an automated RNA purification instrument (Promega Maxwell RSC) and a Maxwell RSC FFPE RNA kit (AS1440, Promega Corporation, Madison, WI) according to the manufacturer’s instructions. Complementary DNA (cDNA)

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Maxwell RSC RNA FFPE Kit

Maxwell RSC RNA FFPE Kit from Promega

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Maxwell RSC RNA FFPE Kit from Promega

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