Maxwell RSC RNA FFPE Kit AS1440 from Promega

Product Specs
ItemMaxwell RSC RNA FFPE Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1440
Quantity48 preps
SourceCell/Tissue, Fixed Tissue
Nucleic Acid TypeTotal RNA
Sample TypeFormalin-fixed, paraffin-embedded tissue

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Prognostic Impact of TERT Promoter Mutations in Adult-Type Diffuse Gliomas Based on WHO2021 CriteriaCancers (Basel)May 27, 2024Yujin Lee, Chul-Kee Park, Sung-Hye Parksections using the Maxwell® RSC DNA FFPE Kit (Promega, Madison, WI, USA) and Maxwell® RSC RNA FFPE Kit (AS1440; Promega, Madison, WI, USA) by the manufacturer’s instructions. The library was generated using SureSelectXT

(22) Quantifying mRNA in Highly Degraded Fixed Tissues by Nanostring Technology: A Comparative StudyMethods and ProtocolsMay 7, 2024Eros Azzalini, Barbara Di Stefano, Vincenzo Canzonieri, Tiziana Venesio, Umberto Miglio, Caterina Marchiò, Anna Sapino, Carlo Previderè, Paolo Fattorini, Serena Boninqualitative results obtained in optimizing the extraction protocol, the Maxwell® RSC RNA FFPE kit (Cat. No. AS1440, Promega, Madison, WI 53711-5399, USA) was chosen for RNA purification in a final volume of 50 µL. One

(23) NGS-Guided Precision Oncology in Breast Cancer and Gynecological Tumors—A Retrospective Molecular Tumor Board AnalysisCancers (Basel)April 19, 2024Niklas Gremke, Fiona R. Rodepeter, Julia Teply-Szymanski, Sebastian Griewing, Jelena Boekhoff, Alina Stroh, Thomas S. Tarawneh, Jorge Riera-Knorrenschild, Christina Balser, Akira Hattesohl, et al.Maxwell RSC FFPE Plus DNA Kit (AS1720). For the FusionPlex™ assay, RNA was extracted using the RSC RNA FFPE Kit (Promega, AS1440). Quantification of nucleic acids was performed using a Quantus Fluorometer with the QuantiFluor ONE dsDNA System

(24) Betanin Attenuates Epigenetic Mechanisms and UV-Induced DNA Fragmentation in HaCaT Cells: Implications for Skin Cancer ChemopreventionNutrientsMarch 16, 2024Afshin Zand, Sodbuyan Enkhbilguun, John M. Macharia, Krisztina Varajti, Istvan Szabó, Gellért Gerencsér, Boglárka Bernadett Tisza, Bence L. Raposa, Zoltán Gyöngyi, Timea Varjashelp of the Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA) using a Maxwell® RSC RNA FFPE Kit (AS1440, Promega, Fitchburg, WI, USA). The process was conducted according to the manufacturer’s instructions.

(25) The Impact of Cancer-Associated Fibroblasts on the Biology and Progression of Colorectal CarcinomasGenes (Basel)February 6, 2024Larissa Maria Henrich, Kristina Greimelmaier, Michael Wessolly, Nick Alexander Klopp, Elena Mairinger, Yvonne Krause, Sophia Berger, Jeremias Wohlschlaeger, Hans-Ulrich Schildhaus, Hideo Andreas Baba, et al.purified from 10 µm thick FFPE sections using the Maxwell® RSC DNA FFPE Kit or Maxwell® RSC RNA FFPE Kit (AS1440, Promega, Madison, WI, USA), according to the manufacturer’s recommendations. RNA was eluted in 50 µL

(26) H2S-Synthesizing Enzymes Are Putative Determinants in Lung Cancer Management toward Personalized MedicineAntioxidants (Basel)December 28, 2023Ana Hipólito, Cindy Mendes, Filipa Martins, Isabel Lemos, Inês Francisco, Fernando Cunha, Teresa Almodôvar, Cristina Albuquerque, Luís G. Gonçalves, Vasco D. B. Bonifácio, et al.(IPOLFG, EPE) upon semiautomatic isolation with the Maxwell purification system (Maxwell RSC RNA FFPE Kit, AS1440, Promega). Complementary DNA (cDNA) was synthesized as mentioned above. SYBR Green PCR master mix (04707516001,

(27) Tumor cell cytoplasmic metallothionein expression associates with differential tumor immunogenicity and prognostic outcome in high-grade serous ovarian carcinomaFrontiers in OncologyNovember 8, 2023Elena Mairinger, Michael Wessolly, Paul Buderath, Sabrina Borchert, Larissa Henrich, Pawel Mach, Julia Steinborn, Rainer Kimming, Bharat Jasani, Kurt Werner Schmid, et al.procedures were performed semi-automatically on the Maxwell purification system (Maxwell RSC RNA FFPE Kit, AS1440, Promega) as specified by the manufacturer. RNA was eluted in 50 µl of ribonuclease (RNAse)-free water

(28) Comparison and Validation of Rapid Molecular Testing Methods for Theranostic Epidermal Growth Factor Receptor Alterations in Lung Cancer: Idylla versus Digital Droplet PCRInternational Journal of Molecular SciencesOctober 27, 2023Camille Léonce, Clémence Guerriau, Lara Chalabreysse, Michaël Duruisseaux, Sébastien Couraud, Marie Brevet, Pierre-Paul Bringuier, Delphine Aude Poncetand Mutation Analysis Serial sections were used for DNA extraction. The Maxwell® RSC RNA FFPE Kit (AS1440, Promega, Charbonnières-les-Bains, France) was used (without DNAse) to obtain total nucleic acid (TNA).

(29) Predictive Genetic Biomarkers for the Development of Peritoneal Metastases in Colorectal CancerInt J Mol SciAugust 15, 2023Danique J. I. Heuvelings, Anne G. W. E. Wintjens, Laura Moonen, Sanne M. E. Engelen, Ignace H. J. T. de Hingh, Liselot B. Valkenburg-van Iersel, Marcel den Dulk, Jan Beckervordersandforth, Sharon G. M. Thijssen, Daphne J. G. Leunissen, et al.isolated using a Maxwell RSC® System for Genomic DNA or RNA Extraction with a FFPE AS1450 kit and FFPE AS1440 kit, respectively (Promega, Madison, WI, USA). A blank control sample was analyzed in parallel to each

(30) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJuly 1, 2023Kim J, Lim J, Yoo ID, Park S, Moon JS.nanostring nCounter gene expression analysis Total RNA was extracted with Maxwell® RSC RNA FFPE Kit (AS1440, Promega, Madison, WI, USA), Maxwell® RSC simplyRNA tissue kit (AS1340, Promega), or Maxwell® RSC simplyRNA cells kit

(31) Evaluation of a Set of miRNAs in 26 Cases of Fatal Traumatic Brain InjuriesInternational Journal of Molecular SciencesJune 29, 2023Serena Bonin, Stefano D’Errico, Caterina Medeot, Carlo Moreschi, Solange Sorçaburu Ciglieri, Michela Peruch, Monica Concato, Eros Azzalini, Carlo Previderè, Paolo Fattorinisubdivided into two sections: the first section refers to the use of the Maxwell® RSC RNA FFPE kit (Cat. No. AS1440, Promega, Madison, WI, USA), in which the sections were de-waxed, digested with proteinase K overnight,

(32) Genomic profiles of IDH-mutant gliomas: MYCN-amplified IDH-mutant astrocytoma had the worst prognosisScientific ReportsApril 25, 2023Kwanghoon Lee, Seong-Ik Kim, Eric Eunshik Kim, Yu-Mi Shim, Jae-Kyung Won, Chul-Kee Park, Seung Hong Choi, Hongseok Yun, Hyunju Lee, Sung-Hye Parktumor RNA was extracted from a paraffin block (tumor fraction: > 90%) using Maxwell® RSC RNA FFPE Kit (AS1440; Promega). The library was generated using SureSelectXT RNA Direct Kit (Agilent, Santa Clara, CA, USA)

(33) Genomic and immune landscape Of metastatic pheochromocytoma and paragangliomaNature CommunicationsFebruary 28, 2023Bruna Calsina, Elena Piñeiro-Yáñez, Ángel M. Martínez-Montes, Eduardo Caleiras, Ángel Fernández-Sanromán, María Monteagudo, Rafael Torres-Pérez, Coral Fustero-Torre, Marta Pulgarín-Alfaro, Eduardo Gil, et al.and Tissue Kit (Qiagen, 69506). RNA from FFPE was extracted using Maxwell RSC RNA FFPE Kit (Promega, AS1440) and from FF with TRIzol Reagent (Invitrogen, 15596026). Germline DNA was extracted either from peripheral blood mononuclear cells

(34) Identification of germline cancer predisposition variants in pediatric sarcoma patients from somatic tumor testingScientific ReportsFebruary 20, 2023Piedad Alba-Pavón, Lide Alaña, Miriam Gutierrez-Jimeno, Susana García-Obregón, Teresa Imízcoz, Elena Panizo, Paula González-Urdiales, Aizpea Echebarria-Barona, Ricardo Lopez Almaraz, Laura Zaldumbide, et al.(Promega, AS1450), Maxwell RSC Tissue DNA Kit (Promega, AS1610), Maxwell RSC RNA FFPE kit (Promega, AS1440) and Maxwell RSC Simply RNA tissue (Promega, AS1340). DNA and RNA were quantified using a Qubit fluorometer

(35) Integrative multi-omics networks identify PKCδ and DNA-PK as master kinases of glioblastoma subtypes and guide targeted cancer therapyNature CancerFebruary 2, 2023Simona Migliozzi, Young Taek Oh, Mohammad Hasanain, Luciano Garofano, Fulvio D’Angelo, Ryan D. Najac, Alberto Picca, Franck Bielle, Anna Luisa Di Stefano, Julie Lerond, et al.Maxwell RSC simplyRNA Tissue Kit (Promega, AS1340) for frozen samples or Maxwell RSC RNA FFPE kit (Promega, AS1440) for FFPE specimens. RNA extracted from both tissues was analyzed using the same workflow. Complementary

(36) Tumor Microenvironment in Male Breast Carcinoma with Emphasis on Tumor Infiltrating Lymphocytes and PD-L1 ExpressionInternational Journal of Molecular SciencesJanuary 3, 2023Iva Brcic, Andrea Maria Kluba, Theresa Marie Godschachner, Christoph Suppan, Peter Regitnig, Nadia Dandachi, Sigurd Friedwald Lax, Marija Balićaccording to the manufacturer’s instructions, using the Maxwell RSC FFPE Plus RNA Purification Kit (AS1440, Promega, Thermo Fischer Scientific, Waltham, MA, USA). 4.4.2. Targeted Next-Generation Sequencing RNA

(37) Implementation of Copy Number Variations-Based Diagnostics in Morphologically Challenging EWSR1/FUS::NFATC2 Neoplasms of the Bone and Soft TissueInternational Journal of Molecular SciencesDecember 19, 2022Iva Brcic, Susanne Scheipl, Marko Bergovec, Andreas Leithner, Joanna Szkandera, Karl Sotlar, Arnold J. Suda, Maria Anna Smolle, Tanja Kraus, Andrew Eric Rosenberg, et al.tumor content. RNA and DNA were isolated using the Maxwell RSC FFPE Plus RNA/DNA Purification Kit (AS1440/AS1720, Promega, Thermo Fischer Scientific, Waltham, MA, USA) according to the manufacturer´s instructions.

(38) Digital gene expression analysis of NSCLC-patients reveals strong immune pressure, resulting in an immune escape under immunotherapyBMC CancerDecember 1, 2022Michael Wessolly, Susann Stephan-Falkenau, Anna Streubel, Marcel Wiesweg, Sabrina Borchert, Elena Mairinger, Jens Kollmeier, Henning Reis, Torsten Bauer, Kurt Werner Schmid, et al.were used for semi-automatic isolation with the Maxwell purification system (Maxwell RSC RNA FFPE Kit, AS1440, Promega). The purification was performed according to the manufacturer’s instructions. RNA was eluted

(39) The Gene Expression Landscape of Prostate Cancer BM Reveals Close Interaction with the Bone MicroenvironmentInternational Journal of Molecular SciencesOctober 27, 2022Alireza Saraji, Kang Duan, Christian Watermann, Katharina Hempel, Marie C. Roesch, Rosemarie Krupar, Janine Stegmann-Frehse, Danny Jonigk, Mark Philipp Kuehnel, Wolfram Klapper, et al.RNAase-free tube. RNA was isolated using the automatic bead-based Maxwell® RSC RNA FFPE Kit (Cat. No: AS1440, Promega, Madison, WI, USA) according to the manufacturer`s manual and guidance. The RNA was eluted

(40) Targeted RNA-sequencing analysis for fusion transcripts detection in tumor diagnostics: assessment of bioinformatic tools reliability in FFPE samplesExploration of Targeted Anti-tumor TherapyOctober 27, 2022Iolanda Capone, Fabio Bozzi, Gian Paolo Dagrada, Paolo Verderio, Elena Conca, Adele Busico, Maria Adele Testi, Valentina Monti, Matteo Duca, Claudia Proto, et al.xylene and alcohols and total nucleic acid was extracted using the Maxwell® RSC RNA FFPE Kit (cat: AS1440, Promega, Milan, Italy) in accordance with the manufacturer’s recommendations. RNA concentration was

Be the First to Write a Review!

Maxwell RSC RNA FFPE Kit

Maxwell RSC RNA FFPE Kit from Promega

Description

Promega

Citations (60)

Reviews

Maxwell RSC RNA FFPE Kit from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map