ADCC Reporter Bioassay, Core Kit G7010 from Promega

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ItemADCC Reporter Bioassay, Core Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7010
Quantity1 each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Glycan pairing in therapeutic IgG orchestrates Fcγ receptor engagement and ADCC: an integrated structure-function approach for thorough evaluation of Fc N-glycans as critical quality attributesMAbsApril 2, 2026Maximilian Meudt, Julia Baumeister, Antoinette Cassiopeia Russell, Lindsey Dzielak, Gale Hansen, Boris Mizaikoff, Sybille Ebert, Frank Rosenau, Michaela Blech, Fabian Higelreceptor that signals through the NFAT response element driving expression of firefly luciferase (Cat. No. G7010, Promega, Fitchburg, WI, USA) as the effector cells. Raji cells were cultivated in RPMI 1640 with L-glutamine

(2) Identification of a potent V3 glycan site broadly neutralizing antibody targeting an N332gp120 glycan-independent epitopeNature ImmunologyFebruary 3, 2026Lutz Gieselmann, Andrew T. DeLaitsch, Malena Rohde, Caelan Radford, Johanna Worczinski, Anna Ashurov, Elvin Ahmadov, Judith A. Burger, Colin Havenar-Daughton, Sharvari Deshpande, et al.through FcγR was assessed using an ADCC reporter bioassay from Promega (Promega, G9790 (FcγRIIIa-F), G7010(FcγRIIIa-V)). CHO cells overexpressing HIV JRFL envelope (CHO-HIV JRFL ENV) were used as targets. HIV

(3) Viral clearance and escape during therapy of COVID-19 outpatients: a prospective cohort studyiScienceNovember 26, 2025Guillaume Martin-Blondel, Paul Burgat, Valentin Leducq, Françoise Porrot, Andrea Cottignies-Calamarte, Alejandro De Cruz, Céline Dorival, Raphaelle Romieu-Mourez, Camille Chaubet, Vincent Cazaentre, et al.assays,Critical commercial assays,Critical commercial assays ,, ADCC Reporter Bioassay,Promega,Cat#G7010 Bright-Glo Luciferase Assay System,Promega,Cat#E2620 U-PLEX Biomarker Group 1 kit,Meso Scale Discovery,Custom

(4) A masking clamp for conditional activation of therapeutic antibodiesFrontiers in ImmunologyOctober 30, 2025Adrian Bloch, Jan Felix Zimmermann, Jan Habermann, Evelyn Ullrich, Harald Kolmarcell-mediated cytotoxicity assay ADCC activity was assessed using the ADCC Reporter Bioassay Kit (G7010, Promega) according to the manufacturer’s guidelines. Briefly, 7,500 SK-BR-3 cells (for trastuzumab

(5) Identification of a broad and potent V3 glycan site bNAb targeting an N332 gp120 glycan-independent epitopebioRxivSeptember 16, 2025Lutz Gieselmann, Andrew T. DeLaitsch, Malena Rohde, Caelan Radford, Johanna Worczinski, Anna Momot, Elvin Ahmadov, Judith A. Burger, Colin Havenar-Daughton, Sharvari Deshpande, et al.through FcγR was assessed using an ADCC reporter bioassay from Promega (Promega G9790 (FcγRIIIa-F), G7010(FcγRIIIa-V)). CHO cells overexpressing HIV JRFL envelope (CHO-HIV JRFL ENV) were used as targets. HIV

(6) Second generation CD2-targeting LFA-3 fusion protein SBT115301 to restore immune homeostasis in autoimmune diseaseiScienceMay 16, 2025Herve Lebrec, John Bui, Jonathan M. Clingan, Jason Do, Jason Dubovsky, Leonard Dragone, Ekaterina Gibiansky, Wing Yu Lam, Katherine Matsuda, Lauren Mihalcik, et al.assays ,, Bio-Glo Luciferase Assay System,Promega,Cat#G7940 "ADCC Reporter Bioassay, V Variant",Promega,Cat#G7010 "ADCC Reporter Bioassay, F Variant",Promega,Cat#G9790 EasySep Human CD2 Positive Selection Kit II,Stemcell Technologies

(7) BA.1 breakthrough infection elicits distinct antibody and memory B cell responses in vaccinated-only versus hybrid immunity individualsiScienceApril 18, 2025Carla Saade, Timothée Bruel, Lou-Léna Vrignaud, Martin Killian, Annabelle Drouillard, Véronique Barateau, Maxime Espi, Natacha Mariano, Charlotte Mignon, Lily Bruyère, et al.#18-5092 Octet® amine-coupling reagents,Sartorius,Cat #18-5095 ADCC Reporter Bioassay,Promega,Cat#G7010 Bright-Glo Luciferase Assay System,Promega,Cat#E2620 ,, Deposited data,Deposited data,Deposited data ,, Raw

(8) A monoclonal anti-hemagglutinin stem antibody modified with zanamivir protects against both influenza A and B virusesProceedings of the National Academy of SciencesApril 15, 2025Xin Liu, Thomas Balligand, Camille Le Gall, Hidde L. Ploeghmoribund behavior. ADCC Reporter Bioassay. Human ADCC Reporter Bioassay, V Variant (catalog number G7010, Promega) and mouse FcγRIV ADCC Bioassay (catalog number M1211, Promega) were performed for the MEDI8852–zanamivir

(9) Long-lasting B cell convergence to distinct broadly reactive epitopes following vaccination with chimeric influenza virus hemagglutininsImmunityApril 8, 2025Jenna J. Guthmiller, Linda Yu-Ling Yu-Ling Lan, Lei Li, Yanbin Fu, Sean A. Nelson, Carole Henry, Christopher T. Stamper, Henry A. Utset, Alec W. Freyn, Julianna Han, et al.Jurkat cells (male) expressing FcgRIIIa and FcgRI with a NFAT-driven luciferase reporter gene,Promega,Cat# G7010 Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(10) Mimicry of molecular glues using dual covalent chimerasNat CommunMarch 24, 2025Eden Kapcan, Karolina Krygier, Maya da Luz, Nickolas J. Serniuck, Ali Zhang, Jonathan Bramson, Anthony F. RulloAF647: 400. CD16α activation assay ADCC was quantified using the ADCC Reporter Bioassay kit (Promega G7010). Target cells were seeded at a density of 2.5 × 104 cells per well in opaque 96 well flat-bottom plates

(11) Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profilesJ Clin InvestMarch 17, 2025Svenja Weiss, Hung-Mo Lin, Eric Acosta, Natalia L. Komarova, Ping Chen, Dominik Wodarz, Ian Baine, Ralf Duerr, Ania Wajnberg, Adrian Gervais, et al.cell-based assay and cocultured with either an FcγRIIa or an FcγRIIIa, CD4+ Jurkat reporter cell line (Promega, G7010), which expresses firefly luciferase upon FcγR activation. For this assay, SARS-CoV-2 spike–expressing

(12) 23ME-01473, an Fc Effector-Enhanced Anti-ULBP6/2/5 Antibody, Restores NK Cell-Mediated Antitumor Immunity through NKG2D and FcγRIIIa ActivationCancer Res CommunMarch 1, 2025Benjamin JS, Jarret A, Bharill S, Fontanillas P, 23andMe Research Team, Yadav S, Sen D, Ayupova D, Kellar D, Tilk S, et al.antibodies were added, and the plates were used with the Promega ADCC Reporter Bioassay (Promega, Cat# G7010) per the manufacturer’s instructions. The relative light units from the background wells, which contained

(13) Targeted recruitment of immune effector cells for rapid eradication of influenza virus infectionsProceedings of the National Academy of SciencesOctober 8, 2024Imrul Shahriar, Mohini Kamra, Ananda Kumar Kanduluru, Charity Lynn Campbell, Thanh Hiep Nguyen, Madduri Srinivasarao, Philip S. LowADCC assay was performed using a commercially available ADCC Reporter Bioassay kit (Promega, cat. no. G7010; Promega Corporation, Madison, WI) with influenza virus neuraminidase (N1)-transfected (NA-HEK) cells

(14) Systematic Preparation of a 66-IgG Library with Symmetric and Asymmetric Homogeneous Glycans and Their Functional EvaluationJournal of the American Chemical SocietyAugust 21, 2024Shino Manabe, Shogo Iwamoto, Satoru Nagatoishi, Asako Hoshinoo, Ai Mitani, Wataru Sumiyoshi, Takashi Kinoshita, Yoshiki Yamaguchi, Kouhei TsumotoReporter Assay According to previous studies,9,46 ADCC was performed using a Promega reporter bioassay kit (Cat # G7010). SK-BR3 (American Type Culture Collection [ATCC], Manassas, VA, USA) cells were maintained in McCoy’s

(15) ABBV-319: a CD19-targeting glucocorticoid receptor modulator antibody-drug conjugate therapy for B-cell malignanciesBloodAugust 15, 2024Chewei Anderson Chang, Ethan Emberley, Aloma L. D'Souza, Weilong Zhao, Cormac Cosgrove, Karen Parrish, Diya Mitra, Elmer Payson, Anatol Oleksijew, Paul Ellis, et al.(PerkinElmer). In vitro antibody-dependent cellular cytotoxicity (ADCC) assays The ADCC Reporter Bioassay (G7010 and G9790, Promega) was performed according to the manufacturer's protocol. The target lymphoma cells

(16) Define Critical Parameters of Trastuzumab-Mediated ADCC Assays via Assay Optimization Processes, Focusing on the Impact of Cryopreserved Effector Cells on Assay PerformanceCancersJune 27, 2024Peng H, Endo Y, Wu WJ.of NFAT signaling in effector cells was measured using the ADCC Reporter Bioassay kit (Promega, Cat# G7010). The ADCC reporter bioassay was performed in a 96-well plate format according to manufacturer’s instructions.

(17) Stress-mediated polysorbate 20 degradation and its potential impact on therapeutic proteinsPharmaceutical ResearchJune 1, 2024Baikuntha Aryal, Mari Lehtimaki, V. Ashutosh Raowas measured using the Promega VEGF bioassay kit (GA2001 and GA2005) and ADCC Reporter Bioassay kit (G7010 and G7018) following the manufacturer’s instructions. T-DM1 ADCC activity Target cells, HER2 + SKBR3

(18) Development of a highly effective combination monoclonal antibody therapy against Herpes simplex virusJournal of Biomedical ScienceMay 28, 2024Narges Seyfizadeh, David Kalbermatter, Thomas Imhof, Moritz Ries, Christian Müller, Leonie Jenner, Elisabeth Blumenschein, Alexandra Yendrzheyevskiy, Frank Grün, Kevin Moog, et al.HSV-1F or HSV-2G infected target cells, followed by incubation with effector Jurkat cells (Promega #G7010) stably expressing FcγRIIIA receptor, V158 (high affinity) variant and the NFAT-luciferase reporter

(19) Characterization of a Trispecific PD-L1 Blocking Antibody That Exhibits EGFR-Conditional 4-1BB Agonist ActivityAntibodies (Basel)April 24, 2024Laura Rubio-Pérez, Susana Frago, Marta Compte, Rocío Navarro, Seandean L. Harwood, Rodrigo Lázaro-Gorines, Marina Gómez-Rosel, Oana Hangiu, Noelia Silva-Pilipich, Lucía Vanrell, et al.flow cytometer (Becton Dickinson). 2.10. ADCC Reporter Bioassay The ADCC reporter bioassay (Promega, cat# G7010) was performed according to the manufacturer’s instructions. Briefly, 3T3 or 3T3EGFR cells (1.2 × 104/well)

(20) Anti-CD99 Antibody Therapy Triggers Macrophage-Dependent Ewing Cell Death In Vitro and Myeloid Cell Recruitment In VivoAntibodies (Basel)March 18, 2024Allison F. O'Neill, Evelyn M. Nguyen, Evelyn D. Maldonado, Matthew R. Chang, Jiusong Sun, Quan Zhu, Wayne A. Marascoassays were performed with the use of an ADCC Reporter Bioassay Core Kit (Promega, Maidson, WI, USA #G7010, G7018). Serial dilutions of NOA2 or an isotype control were plated with Ewing cells, or CD99-negative

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ADCC Reporter Bioassay, Core Kit

ADCC Reporter Bioassay, Core Kit from Promega

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ADCC Reporter Bioassay, Core Kit from Promega

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