ONE-Glo EX Luciferase Assay System E8110 from Promega

Product Specs
ItemONE-Glo EX Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE8110
Quantity10ml
Molecular Weight~64kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A new STAT3-based potency assay for human G-CSF analog therapeuticsJournal of Pharmaceutical and Biomedical AnalysisNovember 15, 2025Tao Xie, Weiming Ouyang, Yaqin Zhang, Phillip Angart, Patrick J. Lynch, David M. FruchtInvivoGen (San Diego, CA, USA). ONE-Glo™ EX Luciferase Assay System was purchased from Promega (Cat# E8110, Madison, WI, USA). All the rhG-CSF therapeutical products were commercially sourced from U.S. pharmacies.

(2) Validation of CCL20-driven CAR-γδ T secreting PD-1 blockade with enhanced trafficking into solid tumoriScienceOctober 17, 2025Dengji Zhang, Yuan Tang, Wei Sun, Yunkun Guan, Yipeng Cheng, Yuqi Zhu, Xiaying Zhao, Xinyi Yang, Huanzhang Zhu70-EK180-96 Human TNF-α ELISA Kit,MultiSciences,Cat#: 70-EK182-96 ONE-Glo™ EX Luciferase Assay System,Promega,Cat#:E8110 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, A549,ATCC,Cat#:

(3) Isocitrate dehydrogenase 1 primes group-3 medulloblastomas for cuproptosisCancer CellJune 9, 2025Derek Dang, Akash Deogharkar, John McKolay, Kyle S. Smith, Pooja Panwalkar, Simon Hoffman, Wentao Tian, Sunjong Ji, Ana P. Azambuja, Siva Kumar Natarajan, et al.plate, and luminescence was measured after 48 hrs using the ONE-Glo EX Luciferase Assay System (Promega, E8110). Apoptosis assay 10,000 cells/well were plated in a 96-well plate and measurements were obtained

(4) Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugateseBioMedicineFebruary 1, 2025Yuichi Adachi, Kotaro Miyake, Kika Ohira, Shingo Satoh, Kentaro Masuhiro, Ryuya Edahiro, Yuya Shirai, Maiko Naito, Yujiro Naito, Takayuki Shiroyama, et al.reader (Molecular Devices). For LLC-luc cells, luciferin solution (ONE-Glo™ EX Luciferase Assay System, E8110, Promega) was added 1 h after NIR irradiation, as per the manufacturer's instructions, and luciferase

(5) GABAergic neuronal lineage development determines clinically actionable targets in diffuse hemispheric glioma, H3G34-mutantCancer cellAugust 27, 2024Liu I, Alencastro Veiga Cruzeiro G, Bjerke L, Rogers RF, Grabovska Y, Beck A, Mackay A, Barron T, Hack OA, Quezada MA, et al.injections, luciferase expression was confirmed using the ONE-Glo EX Luciferase Assay System (Promega Cat#E8110). 12 hours post-nucleofection, cells were injected stereotactically into the right hemisphere of 4–6

(6) The cytokine Meteorin-like inhibits anti-tumor CD8+ T cell responses by disrupting mitochondrial functionImmunityAugust 1, 2024Christopher M. Jackson, Ayush Pant, Wikum Dinalankara, John Choi, Aanchal Jain, Ryan Nitta, Eli Yazigi, Laura Saleh, Liang Zhao, Thomas R. Nirschl, et al.activity using Promega’s ONE-Glo™ EX Luciferase Assay System (Promega, Madison, WI 53711 USA, Catalog E8110) and luminescence measurements were analyzed using the Tecan i-control microplate luminometer (Tecan,

(7) A dual inhibitor of PIP5K1C and PIKfyve prevents SARS-CoV-2 entry into cellsExperimental & molecular medicineAugust 1, 2024Seo Y, Jang Y, Lee SG, Rhlee JH, Kong S, Vo TTH, Kim MH, Lee MK, Kim B, Hong SY, et al.activities were determined by a One-Glo Ex Luciferase Assay system (Promega Corporation, Madison, WI, USA; E8110) and analyzed with cell entry efficiency by GloMax (Promega Corporation) at 24 h postinfection. Cytotoxicity

(8) LncRNAH19 acts as a ceRNA of let-7 g to facilitate endothelial-to-mesenchymal transition in hypoxic pulmonary hypertension via regulating TGF-β signalling pathwayRespiratory ResearchJuly 10, 2024Xin Yu, Jiabing Huang, Xu Liu, Juan Li, Miao Yu, Minghui Li, Yuliang Xie, Ye Li, Junyu Qiu, Zhou Xu, et al.After 48 h, luciferase activity analysis was performed using an ONE-Glo™ EX Luciferase Assay System (E8110, Promega, USA), with Renilla luciferase activity as a control. qRT‒PCR Total RNA from pulmonary arteries

(9) N-glycosylation of the SARS-CoV-2 spike protein at Asn331 and Asn343 is involved in spike-ACE2 binding, virus entry, and regulation of IL-6Microbiology and immunologyMay 1, 2024Das T, Luo S, Tang H, Fang J, Mao Y, Yen HH, Dash S, Shajahan A, Pepi L, Huang S, et al.luminescence units (RLU) of luciferase activity were determined using the ONE-Glo EX Luciferase Assay System (E8110: Promega, Inc.) and a luminometer (BioTek Synergy HT) according to the manufacturer’s instruction (32). 4.6.

(10) SMARCA4 (BRG1) is a haploinsufficient B cell lymphoma tumor suppressor that fine-tunes centrocyte cell fate decisionsCancer CellApril 8, 2024Qing Deng, Priya Lakra, Panhong Gou, Haopeng Yang, Cem Meydan, Matthew Teater, Christopher Chin, Wenchao Zhang, Tommy Dinh, Usama Hussein, et al.cells were used to measure luciferase activities using ONE-Glo™ EX Luciferase Assay System (Promega, E8110) according to the manufacturer’s instructions. Single-nuclei RNA-seq and ATAC-seq (scMO) sequencing Nuclei

(11) Comprehensive mutagenesis maps the effect of all single codon mutations in the AAV2 rep gene on AAV productionElifeMarch 27, 2024Nina K. Jain, Pierce J. Ogden, George M. ChurchRRID:AB_2716687,"1:10,000" Commercial assay or kit,Promega One-Glo EX Luciferase Assay System,Promega,Promega:E8110, Commercial assay or kit,Capsid ELISA,Progen,Progen:PRATV, "Software, algorithm",Python scripts for

(12) Notch1 promotes resistance to cisplatin by up-regulating Ecto-5′-nucleotidase (CD73) in triple-negative breast cancer cellsCell Death DiscoveryJune 30, 2023Yuzhu Qi, Meifang Li, Shaozhong Li, De Zeng, Yingsheng Xiao, Jiwei Li, Qianqian Ye, Edwin Bremer, Guo-jun Zhangthe luciferase activity was measured by using ONE_Glo EX Dual-Luciferase Assay System kit (Promega; E8110). Immunohistochemistry assay and online database analysis TNBC primary lesions were histopathologically

(13) An allosteric pan-TEAD inhibitor blocks oncogenic YAP/TAZ signaling and overcomes KRAS G12C inhibitor resistanceNature cancerJune 1, 2023Hagenbeek TJ, Zbieg JR, Hafner M, Mroue R, Lacap JA, Sodir NM, Noland CL, Afghani S, Kishore A, Bhat KP, et al.(N1110; Promega), for on-target determination of pathway inhibition, or Firefly luciferase reagent (E8110; Promega), for the determination of off-target activity of compounds. Luminesence measurements were

(14) Structural changes in the SARS-CoV-2 spike E406W mutant escaping a clinical monoclonal antibody cocktailCell ReportsJune 1, 2023Amin Addetia, Young-Jun Park, Tyler Starr, Allison J. Greaney, Kaitlin R. Sprouse, John E. Bowen, Sasha W. Tiles, Wesley C. Van Voorhis, Jesse D. Bloom, Davide Corti, et al.and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, ONE-Glo EX,Promega,Cat#E8110 Expifectamine 293,Thermo Fisher,Cat#A14524 Lipofectamine 2000,Thermo Fisher,Cat#11668019 BirA biotin-protein

(15) RNAi-mediated rheostat for dynamic control of AAV-delivered transgenesNature communicationsApril 8, 2023Subramanian M, McIninch J, Zlatev I, Schlegel MK, Kaittanis C, Nguyen T, Agarwal S, Racie T, Alvarado MA, Wassarman K, et al.shaker for 10 min at room temperature, after which 50 μL of ONE-Glo EX luciferase reagent (Promega E8110) was added. Luciferase signals were detected on a SpectraMax M plate reader 5 min later. Ratio of GLuc

(16) Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competenceCell reportsDecember 6, 2022Dai T, Rosario SR, Katsuta E, Sawant Dessai A, Paterson EJ, Novickis AT, Cortes Gomez E, Zhu B, Liu S, Wang H, et al.104 cells). The bioluminescent signals were developed by Luciferase Assay System (Promega, Cat. No. E8110) and measured by plate reader (BioTek) following the manufacturer’s protocol. Photoacoustic imaging

(17) BAF Complex Maintains Glioma Stem Cells in Pediatric H3K27M GliomaCancer discoveryDecember 2, 2022Panditharatna E, Marques JG, Wang T, Trissal MC, Liu I, Jiang L, Beck A, Groves A, Dharia NV, Li D, et al.injections, luciferase expression was confirmed using the ONE-Glo EX Luciferase Assay System (cat. #E8110, Promega). Twelve hours after nucleofection, cells were injected stereotactically into the right pons

(18) Versatile live-attenuated SARS-CoV-2 vaccine platform applicable to variants induces protective immunityiScienceNovember 18, 2022Akiho Yoshida, Shinya Okamura, Shiho Torii, Sayuri Komatsu, Paola Miyazato, Hitomi Sasaki, Shiori Ueno, Hidehiko Suzuki, Wataru Kamitani, Chikako Ono, et al.SuperScript™ III First-Strand Synthesis System for RT-PCR,Invitrogen,Cat# 11904018 ONE-Glo™ EX Luciferase Assay System,Promega,Cat# E8110 DNA Ligation Kit Ver.2.1,TaKaRa Bio,Cat# 6022 ,, Deposited data,Deposited data,Deposited data ,, Viral

(19) Potent and broad neutralization of SARS-CoV-2 variants of concern (VOCs) including omicron sub-lineages BA.1 and BA.2 by biparatopic human VH domainsiScienceAugust 19, 2022Chen C, Saville JW, Marti MM, Schäfer A, Cheng MH, Mannar D, Zhu X, Berezuk AM, Banerjee A, Sobolewski MD, et al.200521 β-galactosidase assay kit,G-Biosciences,Cat# 786-651 ONE-Glo™ EX Luciferase Assay System,Promega,Cat# E8110 Nano-Glo Assay System,Promega,Cat# N1110 Lenti-X™ GoStix™ Plus,TaKaRa,Cat# 631280 BirA biotin-protein

(20) Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidineMolecular cellAugust 4, 2022Arango D, Sturgill D, Yang R, Kanai T, Bauer P, Roy J, Wang Z, Hosogane M, Schiffers S, Oberdoerffer S.,ThermoFisher Scientific,Cat#:AM1940 NorthernMax Prehyb/Hyb buffer,ThermoFisher Scientific,Cat#:AM8677 ONE-Glo EX Reagent,Promega,Cat#:E8110 PowerUp SYBR Green Master Mix,ThermoFisher Scientific,Cat#:A25777 ProSignal Pico ECL Reagent,Genesee

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ONE-Glo EX Luciferase Assay System

ONE-Glo EX Luciferase Assay System from Promega

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ONE-Glo EX Luciferase Assay System from Promega

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