ADCC Reporter Bioassay, Complete (Raji) G7015 from Promega

Product Specs
ItemADCC Reporter Bioassay, Complete (Raji)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7015
Quantity1 each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Improved VSV-Ebola-GP booster vaccination approach promotes antibody affinity maturation and durable anti-Ebola immunity in humansNature ImmunologyMarch 19, 2026Surender Khurana, Olivia Posadas, Lela Kardava, Elizabeth M. Coyle, Supriya Ravichandran, Gabrielle Grubbs, Rosemary McConnell, Nadine Rouphael, Guillaume Poliquin, Joanne M. Langley, et al.albumin. ADCC and ADCP assays ADCC and ADCP assays were performed on the samples with Promega kits G7015 and G9901, respectively. HEK293T cells were transfected with plasmid expressing GP from the EBOV Kikwit

(2) Macrophage-Engaging IgG4 Antibody Triggers Cytotoxicity against Integrin αvβ3+ CancersMolecular Cancer TherapeuticsOctober 27, 2025Joshua P. Reddy, Ziqi Yu, Ryan M. Shepard, Tami Von Schalscha, Rebecca A. Clague, Beatriz P. Peixoto, Stephen J. McCormack, Mark W. Onaitis, Sara M. Weis, David A. Cheresh, et al.measured using the FcγRI ADCP Bioassay (Promega, GA1341), ADCC Reporter Bioassay, V Variant (Promega, G7015), and FcγRlla-H ADCP Bioassay (Promega G9901), following the manufacturer’s protocol. Briefly, H1975

(3) Structure-Based High-Efficiency Homogeneous Antibody Platform by Endoglycosidase Sz Provides Insights into Its Transglycosylation MechanismJACS AuJune 24, 2024Yin-Cheng Hsieh, Hong-Hsiang Guan, Chien-Chih Lin, Teng-Yi Huang, Phimonphan Chuankhayan, Nai-Chi Chen, Nan-Hsuan Wang, Pu-Ling Hu, Yi-Chien Tsai, Yen-Chieh Huang, et al.min. ADCC Assay The ADCC activity was analyzed by the ADCC reporter bioassay complete kit (Promega, G7015) using luciferase reporter cells. The related cell lines MCF7W (OBI-888), SKBR-3 (Herceptin, Perjeta),

(4) Antibody attributes, Fc receptor expression, gestation and maternal SARS-CoV-2 infection modulate HSV IgG placental transferiScienceSeptember 15, 2023Mahant AM, Trejo FE, Aguilan JT, Sidoli S, Permar SR, Herold BC.activation, a biomarker for ADCC, was assayed using the ADCC FcγRIIIa (human) Reporter Bioassay (cat #: G7015, Promega, Madison, WI) with 1:5 dilution of plasma. Fold-induction was calculated relative to luciferase

(5) Lymph Node Follicle‐Targeting STING Agonist Nanoshells Enable Single‐Shot M2e Vaccination for Broad and Durable Influenza ProtectionAdvanced ScienceJune 1, 2023Hsiao‐Han Tsai, Ping‐Han Huang, Leon CW Lin, Bing‐Yu Yao, Wan‐Ting Liao, Chen‐Hsueh Pai, Yu‐Han Liu, Hui‐Wen Chen, Che‐Ming J. HuSurrogate Assay The ADCC reporter bioassay was performed according to the manufacturer's protocol (Cat No. G7015, Promega). Briefly, the MDCK cells were infected by PR8 H1N1 using an infection medium (DMEM containing

(6) Chicken‐derived CD20 antibodies with potent B‐cell depletion activityBritish Journal of HaematologyNovember 1, 2022Karuppiah Chockalingam, Anil Kumar, Jianxun Song, Zhilei ChenExtracellular Substrate and LgBiT Protein. The ADCC Reporter Bioassay, Complete Kit (Raji) (Promega, G7015) containing thaw-and-use Raji (target) cells and FcγRIIIa (V158 variant)-displaying nuclear factor of

(7) Functional and structural modifications of influenza antibodies during pregnancyiScienceApril 15, 2022Madeleine F. Jennewein, Martina Kosikova, Francesca J. Noelette, Peter Radvak, Carolyn M. Boudreau, James D. Campbell, Wilbur H. Chen, Hang Xie, Galit Alter, Marcela F. PasettiCVCL_0006 Madin-Darby canine kidney-SIAT1 cells,Sigma Aldrich,CAT#05071502 Jurkat effector cells,Promega,CAT#: G7015 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pFastBac gp67 vector,GenScript,GenScript propriety ,, Software

(8) Characterisation of a highly potent and near pan-neutralising anti-HIV monoclonal antibody expressed in tobacco plantsRetrovirologyDecember 1, 2021Catherine M. Moore, Melanie Grandits, Clemens Grünwald-Gruber, Friedrich Altmann, Maria Kotouckova, Audrey Y.-H. Teh, Julian K.-C. Maeffector cells (Jurkat cell line stably expressing human FcγRIIIa V158 and NFAT-induced luciferase—cat. #G7015). A three-fold dilution row of each respective bNAb was performed in sterile white flat bottom 96 well

(9) Factor VIII-Fc Activates Natural Killer Cells via Fc-Mediated Interactions With CD16Frontiers in ImmunologyJune 28, 2021H.A. Daniel Lagassé, Louis B. Hopkins, Wojciech Jankowski, Marc G. Jacquemin, Zuben E. Sauna, Basil Goldingfetal calf serum (Gibco #10438-034). ADCC Reporter Bioassay The ADCC Reporter Bioassay (Promega #G7015) is a surrogate assay for monitoring cellular cytotoxicity by NK cells mediated by Fc engagement with

(10) Use of 5‐Thio‐L‐Fucose to modulate binding affinity of therapeutic proteinsBiotechnology and BioengineeringMay 1, 2021Martina Zimmermann, Melanie Nguyen, Christian M. Schultheiss, Harald Kolmar, Aline Zimmercommercially available ADCC Reporter Bioassay Kit according to the manufacturer's instructions (Promega, G7015). The CD20+ target cells (Raji cells) and effector cells (engineered Jurkat cells) were resuspended

(11) Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patientsScience translational medicineOctober 7, 2020Pierce CA, Preston-Hurlburt P, Dai Y, Aschner CB, Cheshenko N, Galen B, Garforth SJ, Herrera NG, Jangra RK, Morano NC, et al.in white, flat-bottomed 96-well plates. Reporter cells expressing human FcγRIIIa V variant (Promega G7015) were added for 6 hours at 37°C, 5% CO2. FcγRIIIa activation was detected by the addition of luciferin

(12) Anti-PD-1 Induces M1 Polarization in the Glioma Microenvironment and Exerts Therapeutic Efficacy in the Absence of CD8 Cytotoxic T CellsClinical cancer research : an official journal of the American Association for Cancer ResearchSeptember 1, 2020Rao G, Latha K, Ott M, Sabbagh A, Marisetty A, Ling X, Zamler D, Doucette TA, Yang Y, Kong LY, et al.using standard murine FcγRIII ADCC effector cells according to manufacturer’s recommendations (Promega, G7015). Briefly, a day before the assay, target cells (EOC20) were plated in fresh medium in a 96-well plate

(13) Targeting a membrane-proximal epitope on mesothelin increases the tumoricidal activity of a bispecific antibody blocking CD47 on mesothelin-positive tumorsmAbsJanuary 1, 2020Hatterer E, Chauchet X, Richard F, Barba L, Moine V, Chatel L, Broyer L, Pontini G, Bautzova T, Juan F, et al.FcγR-IIA (H131) or FcγR-IIIA (V158) reporter cell lines as instructed by the manufacturer (Promega, G9901, G7015). Briefly, 12500 NCI-N87 cells per well were mixed with 75000 engineered-reporter cell lines in which

(14) A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza HemagglutininCell Host & MicrobeOctober 11, 2017Fei Yu et al.Assays,Critical Commercial Assays,Critical Commercial Assays ,, ADCC Reporter Bioassay kit,Promega,Cat# G7015 CytoTox-ONE Homogeneous Membrane Integrity Assay,Promega,Cat# G7890 ,, Deposited Data,Deposited Data,Deposited

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ADCC Reporter Bioassay, Complete (Raji)

ADCC Reporter Bioassay, Complete (Raji) from Promega

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ADCC Reporter Bioassay, Complete (Raji) from Promega

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