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CellTiter-Glo 2.0 Assay
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CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay from Promega
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Citations:
(>1k)
Figures:
(4)
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CellTiter-Glo 2.0 Assay
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Description
The CellTiter-Glo 2.0 Assay provides a homogeneous method to determine the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTiter-Glo 2.0 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G9243
Quantity
500ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (1174)
(1)
Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathways
Redox Report
December 31, 2026
Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan Huang
Madison, WI, USA), whereas cell viability was measured using the
CellTiter-Glo® 2.0 Assay
(catalog no.:
G9241
;
Promega
).
Fluorescence
or
luminescence
signals were detected using a Varioskan LUX plate reader (
Thermo Fisher Scientific
(2)
3D matrix stiffness drives energy metabolism to orchestrate stem cell osteogenesis via microtubule acetylation and mitochondrial dynamics
Bioactive Materials
November 1, 2026
Zhijie Yang, Jing Na, Yu Liu, Gan Yue, Fei Song, Chiyu Li, Qiusheng Shi, Yubo Fan, Lisha Zheng
cells per 100 μl. 100 μl cell suspension and 100 μl CellTiter-Glo® 2.0
Cell Viability Assay
reagent (
G9241
,
Promega
, WI, USA) were mixed rapidly in opaque 96-plates and the luminescent output was detected by
(3)
A critical role for mitochondrial dynamics in cigarette smoke condensate-induced RPE senescence
Free Radical Biology and Medicine
October 1, 2026
Qi Zhou, Zunyi Zhang, Yinga Wu, Chunmin Dong, Chen-Chuan Wu, James T. Handa, Jing Ma, Shusheng Wang
MitoSOX loading. Cellular ATP level was measured as described in the
CellTiter-Glo 2.0 Assay
kit (
Promega
G9242
). Briefly, ARPE-19 cells were seeded using opaque-walled 96-well plates with 100 μl of culture medium,
(4)
An Injectable, Self-Expanding Hemostatic Foam for Field-Compatible Postpartum Hemorrhage Management
Adv Sci (Weinh)
September 27, 2026
Charlotte Meyer, Noël Strasser, Ilakkiya Ravindrarajah, Elsa Wrenger, Kristian Ivkovic, Jan Ševčík, Sima Šarčević, Lenka Červenková, Jáchym Rosendorf, Václav Liška,
et al.
24 h. Cell viability was assessed through the ATP dependent CellTiter‐Glo 2.0
Cell Viability Assay
(#
G9241
,
Promega
, Dübendorf, Switzerland) and cytotoxicity was measured using the LDH dependent CytoTox 96 Cytotoxicity
(5)
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation
Sci Adv
September 25, 2026
Pushpamali De Silva, Samantha Fitzgerald, Jules Cazaubiel, Kristin Qian, Matan Hofree, Jia-Ren Lin, Roxanne Pelletier, Mariana Lopez Leon, Phuong Vu Mai Ho, Yasutoshi Takashima,
et al.
Cell viability was determined using the CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
, catalog no.
G9242
) for 2D cultures and the
CellTiter-Glo 3D Cell Viability Assay
(
Promega
, catalog no.
G9681
) for 3D cultures,
(6)
Autophagy inhibition sensitizes radiotherapy responses in high-grade mutant IDH1 glioma
Nat Commun
September 18, 2026
Felipe J. Núñez, Kaushik Banerjee, Anzar A. Mujeeb, Ava Mauser, Claire E. Tronrud, Ziwen Zhu, Sadhakshi Raghuram, Maya R. Sheth, Jorge Armando Pena Agudelo, Julio Zelaya,
et al.
post-
inhibitor
treatment. Cell viability was evaluated 72 h post-IR treatment using CellTiter-Glo 2.0 (
Promega
,
G9242
)
luminescence
cell viability assay
, following the manufacturer’s protocol. The resulting
luminescence
(7)
A novel p53R273H-Selective Bispecific T-Cell Engager: from Clinical TCR clone to Computational Optimization and Functional Validation
Molecular Therapy Oncology
September 17, 2026
Nhan Huynh, Thu-My Thi Nguyen, Ngoc Thi Bui, Thao-Nguyen Ho Nguyen, Diem T.P. Tran, Hoai-Nghia Nguyen, Cuong Thach Nguyen
and target cells were subjected to
luminescence
-based cytotoxicity
assay
using Celltiter-Glo (
Promega
G9242
). Both
functional assays
were done following the manufacturer’s instructions. In silico peptide evaluation
(8)
KDM4B Maintains Pyruvate Dehydrogenase Complex Expression to Support Mitochondrial Metabolism and Survival in Cancer Cells
Epigenomes
September 16, 2026
Sachiko Sato, Arif Ul Hasan, Mami Obara, Eiichi Taira
CellTiter-Glo Luminescent Cell Viability Assay
Cell viability was independently evaluated using the
CellTiter-Glo Luminescent Cell Viability Assay
(
G9242
,
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. The reagent was added directly
(9)
Functional impact of Nth like DNA glycosylase 1 on mitochondrial dynamics
Nucleic Acids Res
September 15, 2026
Lisa Hubers, Alexander Myhr Skjetne, Luisa Luna, Yohan Lefol, Solveig Osnes Lund, Ane Marit Wågbø, Xavier Renaudin, Annikka Polster, Zoe J da Silva, Anders Knoph Berg-Eriksen,
et al.
iodide positive. Cell viability was measured with the CellTiter-Glo® 2.0
Cell Viability Assay
(
Promega
,
G9243
) at 25% final concentration using SPARK® Multimode Microplate Reader (Tecan) to
measure
luminescence
.
(10)
The long non-coding RNA, CyKILRb, acts as a competing endogenous RNA for tumor suppressive microRNAs to augment oncogenic phenotypes via induction of the PIK3R2/PI3K/AKT axis
Molecular Therapy Nucleic Acids
September 8, 2026
Xiujie Xie, H. Patrick Macknight, Barbara Dziegielewska, Amy L. Lu, Ngoc Thanh Vu, Charles E. Chalfant
Cell Titer-Glo 2.0
Assay
Kit and
Dual-Luciferase Reporter Assay System
were purchased from
Promega
(
G9242
and
E1910
). Biotin labled miR-control and miR-3151-5p were synthesized from Horizon. The
mycoplasma
(11)
Estrogen promotes tumor phenotypes in ER+ and ER- breast cancer through UGDH-GPR30
JCI Insight
September 8, 2026
Meghan J. Price, Annee D. Nguyen, Corinne H. Strawser, Trupti Trivedi, Cesar C.D. Baeta, Catherine Lavau, Jovita K. Byemerwa, Debarati Mukherjee, Suzanne E. Wardell, Sandeep Artham,
et al.
either 1 nM E2 or continued 10% CS media. Cell viability assays were performed using CellTiter-Glo (
G9241
,
Promega
); reading was taken at baseline (pretreatment) and daily until day 6. Wound healing assay Cells
(12)
Comparative in vitro safety assessments of synthetized and plant-derived cannabidiol (CBD), and pharmacokinetics of synthetized CBD: evidence of similar toxicological profiles and accumulation upon repeated oral administration
Front Toxicol
September 4, 2026
E. T. Wong, D. Bovard, W. K. Schlage, W. Xia, H. Isa, O. Sirbu, A. R. Kolli, G. N. Nikolajsen, S. S. Jensen, J. Ho,
et al.
condition. Cell viability, and the EC50, was measured using the CellTiter-Glo® 2.0 ATP assay (Ref.
G9241
,
Promega
, Madison, WI, USA) on Day 14 of treatment. After removing the medium, 50 µL of serum-free medium
(13)
Antibacterial and Anticancer Evaluation of Copper-Aluminum (CuAl) Layered Double Hydroxides (LDH) Synthesized Using Co-Precipitation Method
Int J Nanomedicine
August 26, 2026
Jiayan Shi, Yueran Wang, Mengting Xu, Shunjie Jin, Ying Yin, Kai He Khor, Xuan Zhou, Ali Bahadur, Kamyar Shameli, Sin-Yeang Teow
cytotoxic effect of CuAl LDH was assessed using the
CellTiter-Glo luminescent cell viability assay
(#
G9241
,
Promega
) as previously described.16,17 Briefly, 5 × 103 cells per well (100 μL/well) were seeded onto
(14)
Mesoscale proximity labeling at chromatin leads to identification of small molecule mechanism of action
Nature Communications
August 24, 2026
Cameron J. Douglas, Preston Samowitz, Feifei Tong, Alice Long, Caitlin M. Bradley, Laszlo Radnai, David W. C. MacMillan, Courtney A. Miller, Gavin Rumbaugh, Ciaran P. Seath
the plates, and 100 µL of a room-temperature mixture of
1:1
DPBS and CellTiter-Glo® 2.0 Reagent (cat.
G9242
,
Promega
) was dispensed into each well using a multichannel pipette. Plates were shaken for 2 min at
(15)
A host-centric morphological profiling approach to identify repurposed antiviral drugs
iScience
August 21, 2026
Elin Asp, Jonne Rietdijk, Marianna Tampere, Hanna Axelsson, Duncan Njenda, Swapnil Potdar, Adelinn Kalman, Polina Georgieva, Maris Lapins, Flavio Ballante,
et al.
proteins" ,, SPECs
drug repurposing
library,https://www.specs.net/,N/A CellTiter-Glo,
Promega
,Ref.
G9243
Paraformaldehyde,
Thermo Scientific
,Ref.
28908
BSA,Sigma,Ref. A8022 Hoechst 33342,
Invitrogen
,Ref.
(16)
The requirement of MALT1 activity for the growth of adult T cell leukemia/lymphoma
iScience
August 21, 2026
Ayako Kamiunten, Daisuke Morishita, Takuro Kameda, Midori Sugiyama, Shunsuke Ebara, Yasunori Kogure, Akio Mizutani, Takaharu Hirayama, Tomohiro Kawamoto, Yotaro Ochi,
et al.
commercial
assays
,Critical commercial
assays
,, CellTiter-Glo® 2.0
Cell Viability Assay
,
Promega
,#
G9242
SureSelect
Custom Kit,
Agilent Technologies
,N/A QuantiChrom™ Lactate Dehydrogenase Kit,BioAssay Systems,#D2DH-100
Human
(17)
TP53 mutation drives unique transcriptional and functional vulnerabilities independent of del(17p) in multiple myeloma
iScience
August 21, 2026
Dimitrios Tsallos, Nemo Ikonen, Juho J. Miettinen, Muntasir Mamun Majumder, Samuli Eldfors, Imre Västrik, Alun Parsons, Minna Suvela, Katie Dunphy, Paul Dowling,
et al.
Media,
Cytiva
,Cat# 11778538 Conditioned medium,This paper,– CellTiter-Glo® 2.0 reagent,
Promega Corporation
,Cat#
G9243
Compounds used in this paper are available on Table S7,–,– ,, Critical commercial
assays
,Critical
(18)
Panitumumab-Based EGFR Blockade in SMARCB1-Deficient Renal Medullary Carcinoma: Preclinical Basis and Prospective Clinical Activity
Clin Cancer Res
August 21, 2026
Niki M. Zacharias, Eric S. Rupe, Xinyue Chen, Ritesh R. Kotecha, Damian T. Rieke, Bradley A. McGregor, Pedro Pesquera, Zhiyuan Yu, Abha Grover, Najat C. Daw,
et al.
panitumumab was used as is (saline solution). For
IC50
assays
, CellTiter-Glo® 2.0
Cell Viability assay
(
G9241
,
Promega
, Madison, WI, USA) was used to determine
IC50
of panitumumab on UOK360 and 786-O cells. For
(19)
Mitochondrial profiling across macrophage states reveals inhibition of IL-4/IL-13 reprogramming by the integrated stress response
Sci Adv
August 21, 2026
Joan Blanco-Fernandez, Miriam Lisci, Mads M. Foged, Chloé Chapuis, Tim Pflästerer, Tatjana Kleele, Alexis A. Jourdain
stimulation, ATP was quantified using the
CellTiter-Glo luminescent cell viability assay
kit (
Promega
,
G9242
) according to the manufacturer’s instructions. Briefly, 100 μl of reconstituted CellTiter-Glo Reagent
(20)
Crosstalk between S-nitrosylation and glycation defines a metabolic vulnerability in liver and renal cancers
Nature Communications
August 20, 2026
Chiara Pecorari, Mojca Bratina, Evelyn Gonzalez, Salvatore Rizza, Letizia Incampo, Lina Vardouli, Paola Giglio, Elena Maresca, Zsófia Márta Sztupinszki, Marcus Montanares Kildegaard Nielsen,
et al.
48 or 72 h, and viability was quantified using the CellTiter-Glo® 2D
Cell Viability Assay
(
Promega
,
G9242
) following the manufacturer’s instructions.
Luminescence
was measured using a Victor X4 plate reader
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CellTiter-Glo 2.0 Assay from Promega
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