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CellTiter-Glo 2.0 Assay
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CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay from Promega
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CellTiter-Glo 2.0 Assay
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Description
The CellTiter-Glo 2.0 Assay provides a homogeneous method to determine the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTiter-Glo 2.0 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G9243
Quantity
500ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (1093)
(1)
Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathways
Redox Report
December 31, 2026
Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan Huang
Madison, WI, USA), whereas cell viability was measured using the
CellTiter-Glo® 2.0 Assay
(catalog no.:
G9241
;
Promega
).
Fluorescence
or
luminescence
signals were detected using a Varioskan LUX plate reader (
Thermo Fisher Scientific
(2)
Patient-derived organoids for personalized drug response profiling and multi-omics integration in oral squamous cell carcinoma
iScience
June 19, 2026
Pengyu Li, Nenghao Jin, Ning Zhang, Chunhui Yu, Lingling E, Wei Song, Lei Zhang
Dose-response curves were generated by measuring
organoid
viability using CellTiter-Glo 3D (
Promega
,
G9243
). The normalized area under the curve (
AUC
) and
IC50
values were calculated for each PDO line to assess
(3)
Atypical Tetracyclines Promote Longevity and Ferroptotic Neuroprotection via Translation Attenuation
Aging Cell
June 18, 2026
Khalyd J. Clay, Manuel Sanchez-Alavez, Ian Newman, Na Na, Ana P. Verduzco Espinoza, Alan To, Shannon Saad, Hollis T. Cline, Michael Petrascheck
Cat#156212500) was added to each well, and cell viability was measured using CellTiter‐Glo (
Promega
, Cat#
G9243
).
Luminescence
was measured using
BioTek
Cytation 5
(Agilent). Results are shown as a percentage of
(4)
Integration and validation of complementary ex vivo assays for functional precision oncology
NPJ Precis Oncol
June 17, 2026
Anna Loboda, Jasmina Paluncic, Micaela Freitas, Marko Weidmann, Sonja Herter, Constantia Zeiser, Juliana P. Schulz, Attila Jády, Mirjam Blattner-Johnson, Robert J. Autry,
et al.
treatment effect was quantified as metabolic activity readout using Cell Titer Glo 2.0 (
Promega
, order #
G9242
) according to manufacturer’s instructions. Individual drug
sensitivity
was evaluated using the R/Shiny
(5)
Investigating the role of mimosine-induced genotoxic stress through DNA repair profiling
Mol Ther Nucleic Acids
June 16, 2026
Sai Pallavi Pradeep, Suraj Kalaria, Peter M. Glazer, Raman Bahal
0.125, 0.25, 0.5, and 1 mM). After 24 h, cell viability was measured using CellTiter-Glo (
Promega
, #
G9241
), and
luminescence
was recorded using TECAN infinite 200 PRO M-plex microplate reader. Statistical
(6)
Identification and Validation of 3-Cyano-Quinoline Ligands Targeting Integrin-Linked Kinase (ILK)
Journal of Medicinal Chemistry
June 11, 2026
Francesco Aleksy Greco, Kamal Rayees Abdul Azeez, Marko Mitrović, Saran Aswathaman Sivashanmugam, Martin Peter Schwalm, Franziska Preuss, Deep Chatterjee, Viktoria Morasch, Sebastian Mathea, Thomas Hanke,
et al.
(Promega) Cell viability was determined using the CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
,
G9241
) following manufacture’s protocol. 40 μL of Huh-7 and
A549 cells
at a
cell count
of 2500 cells/well
(7)
Utility and Limitations of the Methyl Diaminobenzene-BODIPY Fluorescent Biosensor for Detecting Methylglyoxal in Cells
ACS Omega
June 9, 2026
Rangsima Ponvattanaporn, Jeremy P. Le, Zhimo Li, Ryan J.D. Rivero, Jeffrey Y.W. Mak, David P. Fairlie, Hamish E.G. McWilliam, Abderrahman Hachani, Alexandra J. Corbett, Timothy Patton
End point viability was determined using the CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
, Cat. #
G9241
) as per the manufacturer’s instructions, with
luminescence measurements
taken on the
FLUOstar Omega
(8)
Combination of Selpercatinib and Trametinib Overcomes Resistance to RET Inhibitors in RET-Mutant Medullary Thyroid Carcinoma
JCO Precis Oncol
June 8, 2026
David Milewski, Arwa Fallatah, Maya Groff, Abdelrahman Rahmy, Sophia R. Khan, Christian Okafor, Brittany Glassberg, Hsien-Chao Chou, Kerstin Heselmeyer-Haddad, Darawalee Wangsa,
et al.
final 1% DMSO concentration in triplicate for 96 hours. CellTiter-Glo (
Promega
, Madison, WI, Cat. No.
G9243
) was used to produce a luminescent readout representative of cell viability. Drug-treated groups were
(9)
Disrupting Notch signalling by a small molecule inhibiting dihydroorotate dehydrogenase activity
Sci Rep
June 6, 2026
Eike-Benjamin Braune, Dirk Wienke, Anita Seshire, Timo Heinrich, Martin Haraldsson, Sonia Lain, Urban Lendahl
(reading out metabolic activity) was determined using the CellTiter-Glo 2.0 kit (CTG,
Promega
, cat #
G9242
) following the manufacturer’s instructions and
luminescence
was measured on a SpectraMaxi3 or Varioscan
(10)
Genotypic and Phenotypic Diversity as a Function of CRISPR-Directed Gene Knock-Out of NRF2 in Pancreatic Adenocarcinoma Cells, a Feasibility Study
Biomolecules
June 3, 2026
London P. McGill, Kelly H. Banas, Gregory Tiesi, Eric B. Kmiec
before being evaluated using CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
, Madison, WI, USA; Cat.
G9241
). A
1:1
cell suspension made from each biological replicate was loaded into a white 96-well plate in
(11)
Development of a Direct-to-Biology Platform to Discover Potent MNK Inhibitors
Journal of Medicinal Chemistry
May 28, 2026
Purav P. Vagadia, Yingyu Mao, Frank Eckerdt, Joshua L. Zhu, Leonidas C. Platanias, Gary E. Schiltz
imager (
Bio-Rad
). Cell viability Cell viability was assessed with CellTiter-Glo® 2.0 (
Promega
, Cat No.
G9241
) on both LN229 and
HEK293 cells
.
HEK293 cells
were also obtained from American Type Culture Collection
(12)
Molecular architecture of the tumor microenvironment caused by BRCA1 and BRCA2 somatic mutations in human lung adenocarcinoma
Elife
May 26, 2026
Gaoming Liao, Xinbin Yang, Qi Liu, Shufeng Nan, Yan Liu, Jinwei Li, Si Huang, Wang Ning, Xionghai Qin, Gang Xu
was measured over a 4-day time course using the
CellTiter-Glo Luminescent Cell Viability Assay
kit (#
G9242
;
Promega
). For this
assay
, 1000 cells were seeded into each well of a 96-well plate containing 100 μL
(13)
Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLC
Frontiers in Immunology
May 25, 2026
Zehao Huang, JunDao He, Yue Li, ZhanYu Xu, Huajian Peng, Xiang Gao, HuaFu Zhou, JianJi Guo, Nuo Yang
Ferroptosis phenotypes were quantified by cell viability (CellTiter-Glo 2.0
Luminescent Assay
,
Promega
G9241
, normalized to vehicle), lipid peroxidation (BODIPY 581/591 C11 probe,
ThermoFisher
D3861
; oxidized-to-reduced
(14)
Tumor-targeted bispecific antibodies effectively inhibit oncogenic pathways while minimizing toxicity
Sci Adv
May 22, 2026
Yvonne T. Kschonsak, Wei-Ching Liang, Joyce Chan, David Kan, Thao Nguyen, Karen Ruiz, Yee-Seir Kee, Wen-Ting K. Tsai, Hongkang Xi, Marina Moskalenko,
et al.
viability was analyzed using the
CellTiter-Glo Luminescent Cell Viability Assay
(
Promega
,
G7570
and
G9241
) for adherent cells or
CellTiter-Glo 3D Cell Viability Assay
(
Promega
,
G9681
) for three-dimensional
(15)
Proteasomal inhibition compromises microvascular integrity via distinct effects on non-immune endothelial cells and immune cells
Frontiers in Immunology
May 19, 2026
Prajakta Sawant, Hannah Wolf, Aleena Mathew, Johanna Bensalel, Julio Gallego-Delgado, Pratyusha Mandal
based on ATP detection using a CellTiter-Glo® 2.0 Cell Viability Assay kit (
Promega Corporation
, Cat#
G9241
) and cell integrity loss was measured using SYTOX™ Green Nucleic Acid Stain (Invitrogen™,
Thermo Fisher Scientific
(16)
Taxane chemotherapy promotes response to TIM-3 checkpoint blockade via STING-mediated ER stress and HMGB1 secretion
Cell Rep Med
May 19, 2026
Alexis Onimus, Daiana Celias, Shiun Chang, Joshua Davis, Jay Mandula, Jie Li, Alycia Gardner, Kay Hänggi, Olabisi Osunmakinde, Hatem Soliman,
et al.
4444557
TaqMan™ Gene Expression Cells-to-CT™ Kit
,
Thermo Fisher
,Cat#
AM1728
CellTiter-Glo 2.0 Assay
,
Promega
,Cat#
G9242
"Duolink® In Situ Proximity
Ligation
Assay
Starter Kit, Red,
Mouse
/Rabbit",
Sigma-Aldrich
,Cat#
DUO92101-1KT
NaveniFlex
(17)
Metabolomic signatures of SSRI exposure during neural differentiation and correlation of lysophosphatidylcholines with early symptoms of neurodevelopmental disorders
EBioMedicine
May 18, 2026
Abishek Arora, Kristina Vacy, Cátia Marques, Mihai-Ovidiu Degeratu, Francesca Mastropasqua, Jenny Humphrey, Xuan Ye, Marika Oksanen, Peter Vuillermin, Anne-Louise Ponsonby,
et al.
0.05) of the reported findings in R (v4.1.2).33 Cellular ATP
assay
The CellTiter Glow 2.0
assay
(
G9242
,
Promega
) was used to quantify the total ATP levels generated by oxidative phosphorylation in response
(18)
SIRT2 mediates integrated stress response by deacetylating and stabilizing 4E-BP1 to suppress translation
EMBO Reports
May 18, 2026
Yanlin Zi, Jiaqi Zhao, Miao Wang, Dan Hou, Richard A Cerione, Hening Lin
Sigma-Aldrich
.
Dual-Luciferase® Reporter Assay System
(
E1910
) and CellTiter-Glo® 2.0
Cell Viability Assay
(
G9241
) were purchased from
Promega
. TM is synthesized as previously described (Jing et al, 2016). Polyethyleneimine
(19)
Discovery and Development of a Potent LIMK2 Isoform-Specific Degrader
ACS Chem Biol
May 15, 2026
Kamal Rayees Abdul Azeez, Hayuningbudi Saraswati, Thorsten Mosler, Thomas Hanke, Hung Ho-Xuan, Noah Neder, Saran Aswathaman Sivashanmugam, Marcel Heinz, Martin-Peter Schwalm, Giulio Giuliani,
et al.
PROTAC on cell viability were determined using the CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
,
G9241
) following the manufacturer’s protocol. 40 μL of HEK293T cells were seeded with 10000 cells/well into
(20)
Discovery of a Highly Potent and Selective ENL Degrader
Journal of Medicinal Chemistry
May 14, 2026
Kaixiu Luo, Zhaoyu Xue, Lihuai Qin, Zhefan Wang, Pengcheng Gao, Ling Xie, Yangzhou Su, Xian Chen, H. Ümit Kaniskan, Hong Wen,
et al.
viability was measured using the
CellTiter-Glo luminescent cell viability assay
kit (
Promega
, Cat. No.
G9241
) according to the manufacturer’s instructions. Surviving cells were calculated as percentage relative
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CellTiter-Glo 2.0 Assay from Promega
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