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CellTiter-Glo 2.0 Assay
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CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay
CellTiter-Glo 2.0 Assay from Promega
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Citations:
(>1k)
Figures:
(4)
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CellTiter-Glo 2.0 Assay
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Description
The CellTiter-Glo 2.0 Assay provides a homogeneous method to determine the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTiter-Glo 2.0 Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G9243
Quantity
500ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (1134)
(1)
Tramadol induced hypoxia signaling and paraptosis-like cell death in breast cancer cells via HIF-1α and ATF4 dependent pathways
Redox Report
December 31, 2026
Zih-Syuan Wu, Shih-Ming Huang, Yi-Hsuan Huang
Madison, WI, USA), whereas cell viability was measured using the
CellTiter-Glo® 2.0 Assay
(catalog no.:
G9241
;
Promega
).
Fluorescence
or
luminescence
signals were detected using a Varioskan LUX plate reader (
Thermo Fisher Scientific
(2)
3D matrix stiffness drives energy metabolism to orchestrate stem cell osteogenesis via microtubule acetylation and mitochondrial dynamics
Bioactive Materials
November 1, 2026
Zhijie Yang, Jing Na, Yu Liu, Gan Yue, Fei Song, Chiyu Li, Qiusheng Shi, Yubo Fan, Lisha Zheng
cells per 100 μl. 100 μl cell suspension and 100 μl CellTiter-Glo® 2.0
Cell Viability Assay
reagent (
G9241
,
Promega
, WI, USA) were mixed rapidly in opaque 96-plates and the luminescent output was detected by
(3)
A host-centric morphological profiling approach to identify repurposed antiviral drugs
iScience
August 21, 2026
Elin Asp, Jonne Rietdijk, Marianna Tampere, Hanna Axelsson, Duncan Njenda, Swapnil Potdar, Adelinn Kalman, Polina Georgieva, Maris Lapins, Flavio Ballante,
et al.
proteins" ,, SPECs
drug repurposing
library,https://www.specs.net/,N/A CellTiter-Glo,
Promega
,Ref.
G9243
Paraformaldehyde,
Thermo Scientific
,Ref.
28908
BSA,Sigma,Ref. A8022 Hoechst 33342,
Invitrogen
,Ref.
(4)
The requirement of MALT1 activity for the growth of adult T cell leukemia/lymphoma
iScience
August 21, 2026
Ayako Kamiunten, Daisuke Morishita, Takuro Kameda, Midori Sugiyama, Shunsuke Ebara, Yasunori Kogure, Akio Mizutani, Takaharu Hirayama, Tomohiro Kawamoto, Yotaro Ochi,
et al.
commercial
assays
,Critical commercial
assays
,, CellTiter-Glo® 2.0
Cell Viability Assay
,
Promega
,#
G9242
SureSelect
Custom Kit,
Agilent Technologies
,N/A QuantiChrom™ Lactate Dehydrogenase Kit,BioAssay Systems,#D2DH-100
Human
(5)
Doxorubicin‐Induced Cytotoxicity in Adipose‐Derived Stem Cells Is Associated With Altered SAPK/JNK Signaling
FASEB J
July 30, 2026
Aleksandra Skubis‐Sikora, Kinga Pogoda‐Mieszczak, Bartosz Sikora, Edyta Bogunia, Aleksandra Bryzek, Piotr Czekaj
well in 96‐well black plates. The effect of Dox was analyzed using the
CellTiter‐Glo 2.0 assay
kit (
G9241
;
Promega
, Madison, WI, USA) according to the manufacturer's protocol. The bioluminescent
CellTiter‐Glo 2.0 assay
(6)
Targeting chordoma via an isocitrate dehydrogenase-1-dependent susceptibility to redox metabolism
Acta Neuropathol
July 23, 2026
Matthew Pun, Akash Deogharkar, Siva Kumar Natarajan, Nicholas Nuechterlein, Wentao Tian, Sunjong Ji, Eleanor Young, Fusheng Yang, Debra Hawes, Anthony C. Andren,
et al.
concentrations of oxalomalate (Cayman, #13,521), following the CellTiter-Glo kit protocol (
Promega
#
G9241
). Glycolysis and mitochondrial
stress tests
Cells were treated with vehicle or
IDH1
inhibitor
seeding
(7)
Simulated Microgravity Reprograms Host Cell Architecture and Mitochondrial Function, Altering Toxoplasma gondii Growth
FASEB Bioadv
July 21, 2026
Musfika Tasnim Shirin, Takafumi Yamada, Daisuke Kondoh, Hiroki Bochimoto, Kentaro Kato
ATP Metabolic activity and bioenergetic levels of cells were measured using CellTiter‐Glo reagent (cat.
G9242
,
Promega
) and Glomax (Promega). For these experiments, cells were collected after 7 days of
incubation
(8)
A novel RNA-binding activity of ECD contributes to U5 snRNP stability and pre-mRNA splicing
Nucleic Acids Res
July 21, 2026
Mohsin Raza, Asher Rajkumar Rajan, Achyuth Kalluchi, Bhopal Mohapatra, Irfana Saleem, Benjamin B Kennedy, Kishor K Bhakat, Hamid Band, M Jordan Rowley, Vimla Band
time points, viable cells were quantified using the CellTiter-Glo 2.0
cell viability assay
(
Promega
#
G9241
) according to the manufacturer’s instructions. Statistical analysis The results are expressed as mean
(9)
Targeted antibody-drug conjugates for rhabdomyosarcoma and other FGFR4-expressing cancers
Cell Rep Med
July 21, 2026
Meijie Tian, Katrina Jia, Jerry T. Wu, Adam T. Cheuk, Siteng Fang, Victor T. Ojo, Eleanor G. Pope, David Milewski, Yong Yean Kim, Hsien-Chao Chou,
et al.
h of
incubation
, cell viability was assessed using the CellTiter-Glo 2.0
Cell Viability Assay
(Cat#
G9242
,
Promega
) according to the manufacturer’s protocol.
Luminescence
was measured on a Spark Multimode Microplate
(10)
Mapping in-cell protein contact sites reveals hijacking of paraspeckles during influenza A virus infection
Nat Microbiol
July 20, 2026
Iuliia Kotova, Lars Mühlberg, Konstantin Gilep, Mira Lea Burtscher, Isabelle Becher, Dingquan Yu, Daniel Ziemianowicz, Stephanie Stanelle-Bertram, Sebastian Beck, Kyungmin Baeg,
et al.
(
10843-1-AP
;
Proteintech
). Cell viability Cell viability was measured using the
CellTiter-Glo 2.0 Assay
Kit (
G9241
;
Promega
). Treated cells in 24-well plates were incubated with reagent directly added to the culture
(11)
Spinal cord injury induces mitochondrial dysfunction and metabolic reprogramming in non-regenerative Xenopus laevis
iScience
July 17, 2026
Miguel E. Domínguez-Romero, Maximiliano Villarreal, Camila Cordero-Véliz, Sebastián Quijada, Francisco Aguirre, Caterina Hernandez, Sol Torruella-Gonzalez, Johany Peñailillo, Verónica Eisner, Paula G. Slater
commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, CellTiter-Glo,
Promega
,Cat#
G9241
; RRID: SCR_014267 RNeasy kit,
QIAGEN
,Cat#
74104
; RRID: SCR_000628 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, "Primers
(12)
Deformability screening identifies NUDT5 as a mediator of cellular mechanobiology
bioRxiv
July 16, 2026
Angelina M. Flores, Jennifer Soto, Navjot Kaur Gill, Ahmed Almunaifi, Chau Ly, Dongping Qi, Ruchira Krishnamurthy, Eleana Parajón, Bobby Tofig, Valeria Garcia,
et al.
(Bio-Rad). Bulk ATP
luminescence
assay
We measured whole-cell ATP levels using the CellTiter-Glo Kit (
Promega
,
G9242
). ATP standard was prepared (
Sigma
,
A7699
) at concentrations from 0.01 μM to 1 μM. Cells (200 k/well)
(13)
Suppression of ATM kinase signaling accelerates cellular senescence
Stem Cell Reports
July 14, 2026
Kei-ichi Ishikawa, Takahiro Shiga, Takumi Hirose, Naoko Kuzumaki, Sakura Miyoshi, Akihiro Yamaguchi, Hidetaka Tamune, Avijite Kumer Sarkar, Kento Nakai, Kazuyoshi Baba,
et al.
System,
Promega
,Cat#
G8090
CellTiter-Blue Cell Viability Assay
,
Promega
,Cat#
G8080
CellTiter-Glo 2.0
Cell Viability Assay
,
Promega
,Cat#
G9241
Human β-amyloid (1–40) ELISA kit,FUJIFILM
Wako
,Cat#
292-62301
Human β-amyloid (1–42) ELISA kit,FUJIFILM
(14)
An Open-Access Dialysis Membrane-Integrated Microfluidic Device for Generating Drug Exposure Profiles Through Molecular-Weight-Dependent Transport
Micromachines (Basel)
July 14, 2026
Hajime Miyashita, Kenta Shinha, Hiroko Nakamura, Moeno Kadoguchi, Hiroshi Arakawa, Hiroshi Kimura
responses were evaluated as cell viability using ATP content as an indicator. The
CellTiter-Glo® 2.0 Assay
(
G9242
,
Promega Corporation
, Madison, WI, USA) was used for the measurements. After completion of drug exposure,
(15)
On the Scope of DCAF1-Recruiting PROTACs Degrading Protein Kinases
Journal of Medicinal Chemistry
July 9, 2026
Janik Weckesser, Nebojša Miletić, Saran Aswathaman Sivashanmugam, Uli Ohmayer, Martin Steger, Bachuki Shashikadze, Paul Gehrtz, Andrea Unzue Lopez, Ansgar Wegener, Timo Yoshua Dietz,
et al.
Titer Glo PROTAC toxicity was determined using the CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
,
G9241
) following manufacture’s protocol. Ten μL of cells at a
cell density
of 2 * 105 cells/mL were seeded
(16)
Chlorella Hot Water Extract Restores Collagen Production in Senescent Fibroblasts Through Reversal of miR-193a-5p-Mediated Translational Repression of COL1A1
Nutrients
July 3, 2026
Sou Kageyama, Yusei Sato, Zenaida Aurea Krizza B. Escareal, Miharu Amano, Yuka Maejima, Yuji Kawabata, Takushi Namba
Measurement Intracellular ATP content was quantified with the
CellTiter-Glo 2.0 assay
kit (Cat. No.
G9241
;
Promega
, Madison, WI, USA) according to the manufacturer’s protocol. ATP output was normalized to total
(17)
Reconstructing the glioblastoma microenvironment in heterotypic 3D spheroids: a multicellular model to study tumor-stromal crosstalk
Front Bioeng Biotechnol
July 3, 2026
Yuliya Nikitina, Alina Kazakova, Anastasia Leonteva, David Sergeevichev, Maria Bogachek, Sergey Vladimirov, Natalia Vasileva, Vladimir Richter, Anna Nushtaeva
metabolic activity of the spheroids was evaluated on day 5 using the
CellTiter-Glo® 2.0 Assay
Kit (#
G9241
,
Promega Corporation
, Madison, WI, USA). The spheroids were transferred to black 96-well plates (Sovtech,
(18)
Inhibition of SIK2 and SIK3 induces adaptive ER-phagy and creates a therapeutic vulnerability in ovarian cancer
ResearchSquare
July 1, 2026
Zhen Lu, Rumeysa Ozyurt, Gamze Bildik, Weiqun Mao, Hailing Yang, Robert Bast
Assays Cell viability was assessed using the
CellTiter-Glo Luminescent Cell Viability Assay
(
Promega
,
G9241
). Between 2,500 and 3,000 cells per well were seeded into 96-well plates and treated 24 hours later
(19)
Clinical and Functional Significance of p38δ (MAPK13) in Lung Cancer
Cancer Genomics - Proteomics
July 1, 2026
MEHEK SUMAR, TIMOTHY P. FLEMING, ROSS M. BREMNER, NARENDRA V. SANKPAL
72 h post-seeding. At each designated time point, CellTiter-Glo (
Promega
, Madison, WI, USA, catalog #
G9242
) was added directly to triplicate wells according to the manufacturer’s protocol.
Luminescence
, which
(20)
Targeted degradation of MDM2 overcomes feedback regulation of p53 signaling in Merkel cell carcinoma models
J Clin Invest
July 1, 2026
Varsha Ananthapadmanabhan, Simone Bruno, Leonard Vonk, Yu-Chen Cheng, Abeba Teshager, Benjamin K. Eschle, Charles L. Howarth, Joana S. Rodrigues, Julia Schnabel, Ananya Kodali,
et al.
cells were treated for the desired time followed by the addition of CellTiter-Glo 2.0 reagent (
Promega
,
G9242
) and
incubation
according to the manufacturer’s protocol.
Luminescence
was measured using a
FLUOstar Omega
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CellTiter-Glo 2.0 Assay from Promega
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