Maxwell RSC Blood DNA Kit AS1400 from Promega

Product Specs
ItemMaxwell RSC Blood DNA Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1400
Quantity48 preps
SourceBlood
Nucleic Acid TypeGenomic DNA
Sample TypeWhole blood

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) Replication kinetics and infectivity of SARS-CoV-2 variants of concern in common cell culture modelsVirology JournalDecember 1, 2022Lena Mautner, Mona Hoyos, Alexandra Dangel, Carola Berger, Anja Ehrhardt, Armin Baikerafter RNA extraction utilising a Maxwell 48 extraction robot with the Maxwell® RSC Blood DNA Kit (#AS1400, Promega GmbH, Mannheim, Germany). Reactions were performed in accordance to the manufacturer’s instructions

(42) Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent featureNature communicationsNovember 22, 2022Rodrigues Lopes I, Alcantara LM, Silva RJ, Josse J, Vega EP, Cabrerizo AM, Bonhomme M, Lopez D, Laurent F, Vandenesch F, et al.L7386) for 30 min at 37 °C. DNA extraction was performed using the Maxwell RSC Blood DNA Kit (Promega, AS1400), DNeasy UltraClean 96 Microbial Kit (QIAGEN, 10196-4), or DNeasy Blood & Tissue Kit (QIAGEN, 69504),

(43) Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinocerosiScienceNovember 18, 2022Zywitza V, Frahm S, Krüger N, Weise A, Göritz F, Hermes R, Holtze S, Colleoni S, Galli C, Drukker M, et al.μL PBS and genomic DNA (gDNA) automatedly extracted using the Maxwell RSC Blood DNA Kit (Promega, #AS1400) and a Maxwell RSC instrument according to the manufacturer’s instructions (quick protocol from point

(44) Genomic characterization of intracranial teratomas using whole genome sequencingFrontiers in OncologyNovember 15, 2022Chao Zhang, Xiaoyu Zhou, Xiang Huang, Xinghua Ding, Yang Wang, Rong Zhangcells. For thawed tumor tissue or blood, DNA was extracted using the Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). For FFPE sections, DNA was

(45) SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African TrypanosomiasiseBioMedicineNovember 1, 2022NÃºria Sima, Annick Dujeancourt-Henry, Blanca Liliana Perlaza, Marie-Noelle Ungeheuer, Brice Rotureau, Lucy Gloverwhole blood and buffy coat simulated samples was conducted with Maxwell RSC DNA blood kit (PROMEGA AS1400). The samples were pre-processed with 300 μL of lysis buffer and 30 μL of Proteinase K solution, vortexed

(46) Integrative genomic analysis of drug resistance in MET exon 14 skipping lung cancer using patient-derived xenograft modelsFrontiers in OncologyOctober 21, 2022Yunhua Xu, Linping Gu, Yingqi Li, Ruiying Zhao, Hong Jian, Wenhui Xie, Liu Liu, Huiwen Wu, Fang Ren, Yuchen Han, et al.tissue specimens and blood were extracted from thawed materials with the Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). DNA of FFPE tissue was extracted

(47) Ex Vivo Fluorescence Confocal Microscopy (FCM) Ensures Representative Tissue in Prostate Cancer Biobanking: A Feasibility StudyInternational Journal of Molecular SciencesOctober 11, 2022Ulf Titze, Johannes Sommerkamp, Clara Stege, Fried Schneider, Christoph Brochhausen, Birte Schulz, Barbara Titze, Furat Abd Ali, Sasa Pokupic, Karl-Dietrich Sievert, et al.(Promega) and incubated for 30 min at 56 °C. Extraction was performed using the Maxwell® RSC Blood DNA Kit (AS1400; Promega) in the Maxwell® RSC 48 extraction instrument (Promega) according to the manufacturer’s instructions

(48) Improved Bladder Tumor RNA Isolation from Archived Tissues Using Methylene Blue for Normalization, Multiplex RNA Hybridization, Sequencing and SubtypingInternational Journal of Molecular SciencesSeptember 6, 2022Stefanie A. Köhler et al.total RNA isolation methodologies were used for comparison: (1) Maxwell RSC DNA Blood kit (Promega, #AS1400) (Method A). For RNA tissue extraction a purchased incubation buffer (Promega, #D920B) was added replacing

(49) Integrative Genomic and Transcriptomic Analysis of Primary Malignant Gliomas Revealed Different Patterns Between Grades and Somatic Mutations Related to Glioblastoma PrognosisFrontiers in Molecular BiosciencesJuly 5, 2022Huawei Jin, Zhenhua Yu, Tian Tian, Guoping Shen, Weian Chen, Miao Fan, Qun He, Dandan Dai, Xuan Zhang, Dawei LiuThermoFisher), or from the snap-frozen tissue and peripheral whole-blood with Maxwell RSC blood DNA kit (cat# AS1400, Promega). Matched peripheral whole blood was used as the normal (germline) control in filtering to

(50) 16S rRNA nanopore sequencing for the diagnosis of ocular infection: a feasibility studyBMJ Open OphthalmologyMay 23, 2022Masatoshi Omi, Yoshiyuki Matsuo, Kaoru Araki-Sasaki, Shimpei Oba, Haruhiko Yamada, Kiichi Hirota, Kanji Takahashibead-beating for 5 min using a vortex mixer. DNA was extracted using the Maxwell RSC Blood DNA Kit (Promega, AS1400) and used as a template to amplify bacterial 16S rRNA genes. PBS solution, which was used as a solvent

(51) Autophagy Blockage Reduces the Incidence of Pancreatic Ductal Adenocarcinoma in the Context of Mutant Trp53Frontiers in Cell and Developmental BiologyMarch 16, 2022Laura Mainz, Mohamed A. F. E. Sarhan, Sabine Roth, Ursula Sauer, Katja Maurus, Elena M. Hartmann, Helen-Desiree Seibert, Andreas Rosenwald, Markus E. Diefenbacher, Mathias T. Rosenfeldtbuffer mix for 10 min at 80°C and subsequent incubation with proteinase K at 65°C overnight (Promega, #AS1400). Targeted deep sequencing using a customer designed Ion Ampliseq panel of Thermo Fisher including exonic

(52) PARP1 Inhibitor and Trabectedin Combination Does Not Increase Tumor Mutational Burden in Advanced Sarcomas-A Preclinical and Translational StudyCancersDecember 15, 2021Pignochino Y, Crisafulli G, Giordano G, Merlini A, Berrino E, Centomo ML, Chiabotto G, Brusco S, Basiricò M, Maldi E, et al.cultured cells (at least 1.5 × 105 cells) and from frozen tissues using the Maxwell® RSC Blood DNA Kit AS1400 (Promega), from FFPE archival tumor biopsy and surgical excision, after microdissection of the tumor

(53) A comprehensive metagenomics framework to characterize organisms relevant for planetary protectionMicrobiomeDecember 1, 2021David C. Danko et al.sequenced and analyzed. Preparation of samples was adapted from the Maxwell RSC Blood DNA protocol (Promega AS1400) on the Maxwell RSC 48 Machine. The samples were first vortexed to homogenize. Maxwell cartridges were

(54) Highly sensitive detection of driver mutations from cytological samples and cfDNA in lung cancerCancer MedicineDecember 1, 2021Kazutaka Fujita, Masayuki Nakayama, Masafumi Sata, Yoshiaki Nagai, Shu Hisata, Naoko Mato, Takuji Suzuki, Masashi Bando, Nobuyuki Hizawa, Koichi Hagiwara1200× g for 3 min (Figure S1A). DNA was extracted from the precipitate using the Promega Maxwell® RSC AS1400 (Promega). Plasma was isolated from blood samples by two times of centrifugation at 260× g for 10 min.

(55) Estimating tumor mutational burden across multiple cancer types using whole-exome sequencingAnnals of Translational MedicineSeptember 1, 2021Chuang Zhou, Song Chen, Fei Xu, Jinwang Wei, Xiaoyu Zhou, Zhiqiang Wu, Longshuan Zhao, Jun Liu, Wenbo GuoWaltham, MA, USA) and from the peripheral whole blood samples using the Maxwell RSC blood DNA kit (cat# AS1400, Promega, Madison, WI, USA). The recovered DNA was sheared to approximately ~200 bp using a Covaris

(56) Predictive Biomarkers of Dicycloplatin Resistance or Susceptibility in Prostate CancerFrontiers in GeneticsJuly 27, 2021Minglu Liu, Xiaoyu Zhou, Jun Liu, Chelong Lu, Guoqing Zhang, Jing Zhang, Shunchang JiaoMagMAX(Cell-Free DNA Isolation Kit (Applied Biosystems(A29319) and leukocyte DNA by Maxwell® RSC Blood DNA Kit (Promega AS1400). cfDNA for WES needed to meet the following conditions: there is an obvious peak in the region of 100–300

(57) A global metagenomic map of urban microbiomes and antimicrobial resistanceCellJune 24, 2021David Danko, Daniela Bezdan, Evan E. Afshin, Sofia Ahsanuddin, Chandrima Bhattacharya, Daniel J. Butler, Kern Rei Chng, Daisy Donnellan, Jochen Hecht, Katelyn Jackson, et al.assays ,, QIAGEN QIAseq FX DNA Library Kit,QIAGEN,ID: 180475 Promega DNA extraction Maxwell kit Blood,Promega,AS1400 Promega DNA extraction Maxwell kit Buccal Swab,Promega,AS1640 Zymo DNA/RNA shield,Zymo Research,R1100-250 MoBio

(58) Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cellsCell stem cellJune 3, 2021Breunig M, Merkle J, Wagner M, Melzer MK, Barth TFE, Engleitner T, Krumm J, Wiedenmann S, Cohrs CM, Perkhofer L, et al.DHS-3501Z LR Clonase II enzyme mix,Life Technologies,Cat# 11791100 Maxwell RSC Blood Kit,Promega,Cat# AS1400 Nextera XT Kit,Illumina,Cat# FC-131-1096 NEBNext Ultra II FS DNA Library Prep Kit,NEB,Cat# E6177 P3

(59) Integrated genomic and transcriptomic analysis suggests KRT18 mutation and MTAP are key genetic alterations related to the prognosis between astrocytoma and glioblastomaAnnals of Translational MedicineMay 14, 2021Zhiyong Li, Yinghui Jin, Qingping Zou, Xiaofeng Shi, Qianchao Wu, Zhiying Lin, Qun He, Guanglong Huang, Songtao Qito © Annals of Translational Medicine. All rights reserved. WES Maxwell RSC blood DNA kit (cat no. AS1400, Promega) was used to extract DNA in snap-frozen tissue and peripheral whole blood, and MagMAX FFPE DNA/RNA Ultra kit

(60) Propensity for somatic expansion increases over the course of life in Huntington diseaseeLifeMay 13, 2021Radhia Kacher, François-Xavier Lejeune, Sandrine Noël, Cécile Cazeneuve, Alexis Brice, Sandrine Humbert, Alexandra Durrkit QIAamp Fast DNA Tissue Kit Qiagen 51404 commercial assay or kit Maxwell RSC Blood DNA kit Promega AS1400 commercial assay or kit Taq DNA Polymerase Qiagen 201205 Additional information Continued on next

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Maxwell RSC Blood DNA Kit

Maxwell RSC Blood DNA Kit from Promega

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Maxwell RSC Blood DNA Kit from Promega

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