CellTox Green Cytotoxicity Assay G8742 from Promega

Product Specs
ItemCellTox Green Cytotoxicity Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8742
Quantity50ml
Molecular Weight~40kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Specific peptide conjugation to a therapeutic antibody leads to enhanced therapeutic potency and thermal stability by reduced Fc dynamicsScientific ReportsOctober 2, 2023Masato Kiyoshi, Makoto Nakakido, Abdur Rafique, Minoru Tada, Michihiko Aoyama, Yosuke Terao, Satoru Nagatoishi, Hiroko Shibata, Teruhiko Ide, Kouhei Tsumoto, et al.were seeded in CTL-Test medium (Cat. No. CTLT-010, CTL) containing Cell Tox Green reagent (Cat. No. G8742, Promega). Serially diluted antibody samples were added. After incubation for 24 h at 37 °C, fluorescence

(2) A quest for the stereo-electronic requirements for selective agonism for the neurotrophin receptors TrkA and TrkB in 17-spirocyclic-dehydroepiandrosterone derivativesFrontiers in Molecular NeuroscienceSeptember 28, 2023Daniele Narducci, Despoina Charou, Thanasis Rogdakis, Ioanna Zota, Vivi Bafiti, Maria Zervou, Theodora Katsila, Achille Gravanis, Kyriakos C. Prousis, Ioannis Charalampopoulos, et al.4,691), and total Erk1/2 (1:1000, Cell Signaling, cat# 9,194). 2.2.1.3. CellTox assay CellTox assay (G8742, Promega, Leiden, Belgium) was used to assess the survival of PC12 cells under serum deprivation conditions.

(3) Neurotrophin Analog ENT-A044 Activates the p75 Neurotrophin Receptor, Regulating Neuronal Survival in a Cell Context-Dependent MannerInternational Journal of Molecular SciencesJuly 20, 2023Maria Anna Papadopoulou, Thanasis Rogdakis, Despoina Charou, Maria Peteinareli, Katerina Ntarntani, Achille Gravanis, Konstantina Chanoumidou, Ioannis Charalampopoulosfraction. 4.6. Cell Tox Assay After 24 h of treatments, we used the CellTox™ Green Cytotoxicity Assay kit (G8742, Promega Corporation, Maddison, WI, USA) to assess the survival of NIH-3T3, PC12, HEK293T and P7 hippocampal

(4) Wnt signaling preserves progenitor cell multipotency during adipose tissue developmentNature MetabolismJune 19, 2023Zinger Yang Loureiro, Shannon Joyce, Tiffany DeSouza, Javier Solivan-Rivera, Anand Desai, Pantos Skritakis, Qin Yang, Rachel Ziegler, Denise Zhong, Tammy T. Nguyen, et al.assays CellTiter-Glo 2.0 cell viability assay (cat. no. G9243, Promega) and CellTox Green cytotoxicity assay (cat. no. G8742, Promega) were performed according to manufacturer’s instruction and the fluorescence and luminescence

(5) G9a/GLP inhibition during ex vivo lymphocyte expansion increases in vivo cytotoxicity of engineered T cells against hepatocellular carcinomaNature communicationsFebruary 2, 2023Lam MSY, Reales-Calderon JA, Ow JR, Aw JJY, Tan D, Vijayakumar R, Ceccarello E, Tabaglio T, Lim YT, Chien WL, et al.CellTox cytotoxicity assay Cytotoxic effects were analyzed using 2D CellTox Green Cytotoxicity Assays (G8742, Promega) according to the manufacturer’s protocol. Briefly, 5000 HBV-producing HepG2.2.15 hepatoma

(6) Pharmacological Profiling of a Brugia malayi Muscarinic Acetylcholine Receptor as a Putative Antiparasitic TargetAntimicrobial Agents and ChemotherapyJanuary 24, 2023Kendra J. Gallo, Nicolas J. Wheeler, Abdifatah M. Elmi, Paul M. Airs, Mostafa Zamaniantwo replicates with high technical replication were run for motility assays. CellTox (catalog number G8742; Promega) staining was performed at 48 h, following the kit protocol except with half the recommended

(7) Characterization of regulatory transcriptional mechanisms in hepatocyte lipotoxicityScientific ReportsDecember 1, 2022Joaquín Pérez-Schindler, Elyzabeth Vargas-Fernández, Bettina Karrer-Cardel, Danilo Ritz, Alexander Schmidt, Christoph Handschincontrol. Cytotoxicity assay Cytotoxicity was measured with CellTox™ Green Cytotoxicity Assay (Promega, #G8742), according to the manufacturer’s instructions. Intracellular lipid staining Cells were incubated

(8) Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexesGenome biologyAugust 9, 2022Soto I, Couvillion M, Hansen KG, McShane E, Moran JC, Barrientos A, Churchman LS.The toxicity of the drug was measured using the CellTox™ Green Cytotoxicity Assay from Promega (CAT G8742) as described in the instructions. In brief, the dye was mixed with the assay buffer to gain a 5× concentrated

(9) Cooperation of Striatin 3 and MAP4K4 promotes growth and tissue invasionCommunications BiologyAugust 8, 2022Jessica Migliavacca, Buket Züllig, Charles Capdeville, Michael A. Grotzer, Martin BaumgartnerCellTox green cytotoxicity assay Cytotoxicity was determined using the CellToxTM Green Cytotoxicity Assay (G8742, Promega, Dübendorf, Switzerland), according to the manufacturer’s instructions. 2500 DAOY cells were

(10) Development and Biological Characterization of a Novel Selective TrkA Agonist with Neuroprotective Properties against Amyloid ToxicityBiomedicinesMarch 6, 2022Rogdakis T, Charou D, Latorrata A, Papadimitriou E, Tsengenes A, Athanasiou C, Papadopoulou M, Chalikiopoulou C, Katsila T, Ramos I, et al.(1:1000, 4695S, Cell Signaling Technology, Danvers, MA, USA). 2.11. CellTox Assay CellTox assay (G8742, Promega Corporation, Maddison, WI, USA) was used to assess survival of PC12 cells under serum deprivation

(11) Interferon-γ Preferentially Promotes Necroptosis of Lung Epithelial Cells by Upregulating MLKLCellsFebruary 6, 2022Hao Q, Shetty S, Tucker TA, Idell S, Tang H.experiments. Real time necroptosis is monitored by a 0–10 h period using the CellTox™ green cytotoxicity assay (no. G8742, Promega, Madison, WI, USA) according to the manufacturer’s protocol. The assay system uses an asymmetric

(12) Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionPLOS ONESeptember 29, 2021Jennifer Grundman, Brian Spencer, Floyd Sarsoza, Robert A. Rissmanwith the R package ggplot2. Cytotoxicity The CellTox™ Green Cytotoxicity Assay (Promega, cat no. G8742) was used according to the manufacturer’s instructions to assess cytotoxicity as the result of LV-tau

(13) Heme Oxygenase-1 at the Nexus of Endothelial Cell Fate Decision Under Oxidative StressFrontiers in Cell and Developmental BiologySeptember 14, 2021Sindhushree Raghunandan, Srinivasan Ramachandran, Eugene Ke, Yifei Miao, Ratnesh Lal, Zhen Bouman Chen, Shankar Subramaniammanufacturer’s instructions. Cell-Tox Green Assay Cell toxicity was assayed with Cell-Tox reagent (G8742, Promega; Madison, WI, United States) at the end of 12 and 24 h of induction by microscopy. Inomycin

(14) Chromosomally unstable tumor cells specifically require KIF18A for proliferationNature communicationsFebruary 22, 2021Marquis C, Fonseca CL, Queen KA, Wood L, Vandal SE, Malaby HLH, Clayton JE, Stumpff J.statistical significance using Prism 7. For cytotoxicity assays, CellTox™ Green Dye (Promega, Cat no. G8742) was added to cell media prior to imaging, and the number of cells/mm2 was recorded for both GFP and

(15) Physicochemical and Biological Performance of Aloe Vera-Incorporated Native Collagen FilmsPharmaceuticsDecember 2, 2020Mireia Andonegi, Ainhoa Irastorza, Ander Izeta, Koro de la Caba, Pedro Guerrerowas assessed according to plasma membrane integrity. The Cell-Tox Green Cytotoxicity Assay (Promega #G8742) was used for this purpose. To this end, the protocol recommended by the manufacturer was followed.

(16) Real-time sensing of MAPK signaling in medulloblastoma cells reveals cellular evasion mechanism counteracting dasatinib blockade of ERK activation during invasionNeoplasiaOctober 1, 2020Marc Thomas Schönholzer, Jessica Migliavacca, Elena Alvarez, Karthiga Santhana Kumar, Anuja Neve, Alexandre Gries, Min Ma, Michael A. Grotzer, Martin Baumgartnermedium containing increasing concentrations of drugs, complemented with 1:500 of CellTox™ Green Dye (G8742, Promega) as described by the manufacturer. The fluorescence levels representing the nonviable cell

(17) The Contribution of IgG Glycosylation to Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement-Dependent Cytotoxicity (CDC) in Hashimoto’s Thyroiditis: An in Vitro Model of Thyroid AutoimmunityBiomoleculesJanuary 22, 2020Marta Ząbczyńska, Katarzyna Polak, Kamila Kozłowska, Grzegorz Sokołowski, Ewa Pochećreleased from the dead cells into the medium (CellTox Green Cytotoxicity Assay, Promega, Madison, WI, USA; G8742) according to the manufacturer’s instructions. Fluorescence was measured at 485 nm Ex/535 nm Em using

(18) Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell LineBrain sciencesMay 15, 2019Chiavari M, Ciotti GMP, Navarra P, Lisi L.USA) and the number of nonviable cells was detected using CellTox™ Green Cytotoxicity Assay (Cat. No.: G8742—Promega, Madison, WI, USA). The assays were conducted following the manufacturer’s instructions. 2.5.

(19) Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S PhasePLOS ONEDecember 14, 2016Irina A. Okkelman, Ruslan I. Dmitriev, Tara Foley, Dmitri B. Papkovskyproliferation in the intestinal organoids. Methods Materials CellTox Green Cytotoxicity Assay kit (G8742) was from Promega (MyBio, Ireland). Tetramethylrhodamine methyl ester (TMRM) (T-668), cholera toxin

(20) Assessing Combinational Drug Efficacy in Cancer Cells by Using Image-based Dynamic Response AnalysisCancer InformaticsMarch 9, 2016Chao Sima, Jianping Hua, Milana Cypert, Tasha Miller, Heather M. Wilson-Robles, Jeffrey M. Trent, Edward R. Dougherty, Michael L. Bittnernm). Each well also contained 0.0125% CellTox™ green (CTG) dye for measuring cell death (Promega Inc. G8742). This cyanine dye preferentially stains the dead cell DNA when cell membrane integrity is compromised,

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CellTox Green Cytotoxicity Assay

CellTox Green Cytotoxicity Assay from Promega

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CellTox Green Cytotoxicity Assay from Promega

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