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CellTox Green Cytotoxicity Assay
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CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay from Promega
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Citations:
(33)
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CellTox Green Cytotoxicity Assay
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Description
The CellTox Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTox Green Cytotoxicity Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8742
Quantity
50ml
Molecular Weight
~40kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (33)
(1)
Targeting p75NTR activity alleviates the neurotoxic effect of high glucose on iPSC-derived dopaminergic neurons
Stem Cell Research & Therapy
March 21, 2026
Konstantina Chanoumidou, Ioanna Zota, Maria Anna Papadopoulou, Chrystalla Konstantinou, Alexandros Tsimpolis, Electra Tsagliotis, Maria Tziortziou, Katerina Ntarntani, Anne Grünewald, Matthieu David Lavigne,
et al.
1 µM BNN27 for 48 h and the effect on cell viability was evaluated with the Celltox
assay
(
Promega
,
G8742
). Celltox cytotoxicity
assay
Celltox
assay
(
G8742
, Promega Corporation, Maddison, WI, USA) was used
(2)
p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease
Alzheimer's Research & Therapy
February 20, 2026
Maria Anna Papadopoulou, Konstantina Chanoumidou, Maria Peteinareli, Electra Tsaglioti, Konstantina Michalaki, Matthieu D. Lavigne, Ioannis Charalampopoulos
48 h. Cell tox
assay
After 24 h of treatments, we used the
CellTox™ Green Cytotoxicity Assay
kit (
G8742
,
Promega Corporation
, Madison, WI, USA) to assess the survival of hNSCs in the presence or absence of
(3)
Acidosis Forces Fatty Acid Uptake and Metabolism in Cancer Cells Regardless of Genotype
Advanced Science
July 29, 2025
Sébastien Ibanez, Maria Virginia Giolito, Sultan Al‐Siyabi, Kristian Serafimov, Florine Laloux, Emeline Dierge, Léo Aubert, Céline Guilbaud, Selena Kaye, Paula Varon,
et al.
human
serum. Cell toxicity was assessed for all experiments using the
CellTox Green Cytotoxicity Assay
(
G8742
;
Promega
) by measuring
fluorescence
levels at the Spectramax device (Molecular Devices). Pictures of
(4)
Multimodal beneficial effects of BNN27, a nerve growth factor synthetic mimetic, in the 5xFAD mouse model of Alzheimer's disease
Molecular psychiatry
June 1, 2025
Kokkali M, Karali K, Thanou E, Papadopoulou MA, Zota I, Tsimpolis A, Efstathopoulos P, Calogeropoulou T, Li KW, Sidiropoulou K,
et al
.
end
labeling
(
TUNEL
, 11684795910,
Roche
, Basel, Switzerland) and
Celltox Green Cytotoxicity Assay
(
G8742
,
Promega
), respectively, according to the manufacturer's instructions.
Immunofluorescence
Free-floating
(5)
Impaired cell viability and mitochondrial respiration by disperse textile dyes
Frontiers in Allergy
April 24, 2025
Lizette M. Cortes, Nelson R. Vinueza
occur as a result of cell death after disperse dyes exposure, we used
CellTox Green Cytotoxicity Assay
(
G8742
Promega
). IPEC-J2 cells were adjusted to 20,000 cells/well, plated on 50 µl of cells to a black 96 well
(6)
Development of Layer-by-Layer Magnetic Nanoparticles for Application to Radiotherapy of Pancreatic Cancer
Molecules
March 20, 2025
Nobuyoshi Fukumitsu, Yoshitaka Matsumoto, Lili Chen, Yu Sugawara, Nanami Fujisawa, Eri Niiyama, Sosuke Ouchi, Emiho Oe, Takashi Saito, Mitsuhiro Ebara
and changes in the number of dead cells were evaluated using the
CellTox Green Cytotoxicity Assay
(
G8742
,
Promega
, Madison, WI, USA) according to the manufacturer’s protocol. The
assays
were conducted in triplicate,
(7)
Human Monocyte-Derived Macrophages Demonstrate Distinct Responses to Ambient Particulate Matter in a Polarization State- and Particle Seasonality-Specific Manner
Chemical Research in Toxicology
January 20, 2025
Timothy R. Smyth, Stephanie Brocke, Yong Ho Kim, Cara Christianson, Kasey D. Kovalcik, Joseph Patrick Pancras, Michael D. Hays, Weidong Wu, Zhen An, Ilona Jaspers
and
monocyte
base media. Cytotoxicity Measurements The
CellTox Green Cytotoxicity Assay
(
Promega
G8742
) was performed according to manufacturer’s instruction to determine cytotoxicity. Briefly, following
(8)
Giardia antagonizes beneficial functions of indigenous and therapeutic intestinal bacteria during protein deficiency
Gut Microbes
December 31, 2024
Aadra P. Bhatt, Jason W. Arnold, Muyiwa Awoniyi, Shan Sun, Verônica Feijoli Santiago, Deniz Coskuner, Pedro Henrique Quintela, Kenneth Walsh, Jamie Xiao, Renay Ngobeni-Nyambi,
et al.
comparisons. Toxicity, viability, and permeability
assays
Toxicity was quantified using CellTox Green (
Promega
G8742
) per manufacturer’s directions. Briefly, at end of time course,1 µL CellTox Green was added into apical
(9)
Targeted dephosphorylation of SMAD3 as an approach to impede TGF-β signaling
iScience
August 16, 2024
Brewer A, Zhao JF, Fasimoye R, Shpiro N, Macartney TJ, Wood NT, Wightman M, Alessi DR, Sapkota GP.
analyze protein bands by
densitometry
. Cell cytotoxicity
assay
CellTox Green
Assay
(
Promega
, Cat. #
G8742
) was used to assess the cytotoxicity of BDPIC and HDPIC in A549 and U2OS cells at the indicated concentrations
(10)
Divergent responses of human intestinal organoid monolayers using commercial in vitro cytotoxicity assays
PLOS ONE
June 10, 2024
Miranda A. Lewis, Ketki Patil, Khalil Ettayebi, Mary K. Estes, Robert L. Atmar, Sasirekha Ramani
([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] or MTS;
Promega
,
G3580
),
CellTox™ Green Cytotoxicity Assay
(CellTox;
Promega
,
G8742
), and CellTiter-Glo® 2.0
Cell Viability Assay
(CellTiter-Glo;
Promega
,
G9242
). HIO cells were seeded
(11)
Cryptic splicing of stathmin-2 and UNC13A mRNAs is a pathological hallmark of TDP-43-associated Alzheimer’s disease
Acta Neuropathologica
June 1, 2024
Ana Rita Agra Almeida Quadros, Zhaozhi Li, Xue Wang, I. Sandra Ndayambaje, Sandeep Aryal, Nandini Ramesh, Matthew Nolan, Rojashree Jayakumar, Yi Han, Hannah Stillman,
et al.
analysis. Cell
toxicity assay
Cell toxicity was assessed using the CellTox Green
Toxicity assay
(
Promega
, Cat#
G8742
) according to manufacturer’s instructions. Cells were seeded in a 96-well plate, and toxicity measured
(12)
Mapping the substrate landscape of protein phosphatase 2A catalytic subunit PPP2CA
iScience
March 15, 2024
Brewer A, Sathe G, Pflug BE, Clarke RG, Macartney TJ, Sapkota GP.
analyze protein bands by
densitometry
. Cell cytotoxicity
assay
CellTox Green
Assay
(
Promega
, Cat. #
G8742
) was used to assess the cytotoxicity of dTAG-13-mediated dTAG-PPP2CA degradation in dTAG/dTAGPPP2CA
(13)
Changes in lipid composition of host-derived extracellular vesicles following Salmonella infection
Microbiology Spectrum
January 11, 2024
Lisa E. Emerson, Chanel A. Mosby, Samantha Enslow, Winnie W. Hui, Melissa K. Jones, Mariola J. Ferraro
above, cell cytotoxicity was determined using the
Promega
CellTox Green Cytotoxicity Assay
(product #:
G8742
). CellTox Green Reagent was diluted according to the Endpoint Method protocol provided by
Promega
. Four
(14)
Specific peptide conjugation to a therapeutic antibody leads to enhanced therapeutic potency and thermal stability by reduced Fc dynamics
Scientific Reports
October 2, 2023
Masato Kiyoshi, Makoto Nakakido, Abdur Rafique, Minoru Tada, Michihiko Aoyama, Yosuke Terao, Satoru Nagatoishi, Hiroko Shibata, Teruhiko Ide, Kouhei Tsumoto,
et al.
were seeded in
CTL-Test medium
(Cat. No.
CTLT-010
,
CTL
) containing Cell Tox Green reagent (Cat. No.
G8742
,
Promega
). Serially diluted antibody samples were added. After
incubation
for 24 h at 37 °C,
fluorescence
(15)
A quest for the stereo-electronic requirements for selective agonism for the neurotrophin receptors TrkA and TrkB in 17-spirocyclic-dehydroepiandrosterone derivatives
Frontiers in Molecular Neuroscience
September 28, 2023
Daniele Narducci, Despoina Charou, Thanasis Rogdakis, Ioanna Zota, Vivi Bafiti, Maria Zervou, Theodora Katsila, Achille Gravanis, Kyriakos C. Prousis, Ioannis Charalampopoulos,
et al.
4,691), and total Erk1/2 (
1:1000
, Cell Signaling, cat# 9,194). 2.2.1.3. CellTox
assay
CellTox
assay
(
G8742
,
Promega
, Leiden, Belgium) was used to assess the survival of
PC12 cells
under serum deprivation conditions.
(16)
Neurotrophin Analog ENT-A044 Activates the p75 Neurotrophin Receptor, Regulating Neuronal Survival in a Cell Context-Dependent Manner
International journal of molecular sciences
July 20, 2023
Papadopoulou MA, Rogdakis T, Charou D, Peteinareli M, Ntarntani K, Gravanis A, Chanoumidou K, Charalampopoulos I.
fraction. 4.6. Cell Tox
Assay
After 24 h of treatments, we used the
CellTox™ Green Cytotoxicity Assay
kit (
G8742
,
Promega Corporation
, Maddison, WI, USA) to assess the survival of NIH-3T3, PC12, HEK293T and P7 hippocampal
(17)
Wnt signaling preserves progenitor cell multipotency during adipose tissue development
Nature Metabolism
June 19, 2023
Zinger Yang Loureiro, Shannon Joyce, Tiffany DeSouza, Javier Solivan-Rivera, Anand Desai, Pantos Skritakis, Qin Yang, Rachel Ziegler, Denise Zhong, Tammy T. Nguyen,
et al.
assays CellTiter-Glo 2.0
cell viability assay
(cat. no.
G9243
,
Promega
) and
CellTox Green cytotoxicity assay
(cat. no.
G8742
,
Promega
) were performed according to manufacturer’s instruction and the
fluorescence
and
luminescence
(18)
G9a/GLP inhibition during ex vivo lymphocyte expansion increases in vivo cytotoxicity of engineered T cells against hepatocellular carcinoma
Nature communications
February 2, 2023
Lam MSY, Reales-Calderon JA, Ow JR, Aw JJY, Tan D, Vijayakumar R, Ceccarello E, Tabaglio T, Lim YT, Chien WL,
et al
.
CellTox cytotoxicity
assay
Cytotoxic effects were analyzed using 2D CellTox Green Cytotoxicity
Assays
(
G8742
,
Promega
) according to the manufacturer’s protocol. Briefly, 5000 HBV-producing HepG2.2.15
hepatoma
(19)
Pharmacological Profiling of a Brugia malayi Muscarinic Acetylcholine Receptor as a Putative Antiparasitic Target
Antimicrobial agents and chemotherapy
January 24, 2023
Gallo KJ, Wheeler NJ, Elmi AM, Airs PM, Zamanian M.
two replicates with high technical replication were run for motility
assays
. CellTox (catalog number
G8742
;
Promega
)
staining
was performed at 48 h, following the kit protocol except with half the recommended
(20)
Characterization of regulatory transcriptional mechanisms in hepatocyte lipotoxicity
Scientific Reports
December 1, 2022
Joaquín Pérez-Schindler, Elyzabeth Vargas-Fernández, Bettina Karrer-Cardel, Danilo Ritz, Alexander Schmidt, Christoph Handschin
control. Cytotoxicity assay Cytotoxicity was measured with
CellTox™ Green Cytotoxicity Assay
(
Promega
, #
G8742
), according to the manufacturer’s instructions. Intracellular lipid
staining
Cells were incubated
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CellTox Green Cytotoxicity Assay from Promega
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