Maxwell RSC DNA FFPE Kit AS1450 from Promega

Product Specs
ItemMaxwell RSC DNA FFPE Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1450
Quantity48 preps
Molecular Weight~85kDa
SourceCell/Tissue, Fixed Tissue
Nucleic Acid TypeGenomic DNA
Sample TypeFormalin-fixed, paraffin-embedded tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Improving Time-to-Treatment for Advanced Non-Small Cell Lung Cancer Patients through Faster Single Gene EGFR Testing Using the Idyllaâ„¢ EGFR Testing PlatformCurrent OncologyOctober 18, 2022Norbert Banyi, Deepu Alex, Curtis Hughesman, Kelly McNeil, Diana N. Ionescu, Carmen Ma, Stephen Yip, Barbara Meloskyresuspended in 250 μL of lysis buffer (ref MC118A) plus proteinase K (Maxwell RSC DNA FFPE kit ref AS1450 Promega Biotechnology, Madison, WI, USA). The sample was digested overnight and then loaded onto the

(22) ACTH-secreting pituitary carcinoma with TP53, NF1, ATRX and PTEN mutations Case report and review of the literatureEndocrineApril 1, 2022Piotr Sumislawski, Roman Rotermund, Silke Klose, Anne Lautenbach, Annika K. Wefers, Celina Soltwedel, Behnam Mohammadi, Frank Jacobsen, Christian Mawrin, JÃ¶rg Flitsch, et al.using a fine needle under an inverted microscope. The DNA was isolated using Maxwell® RSC DNA FFPE Kit (AS1450, Promega). Next-generation-sequencing DNA panel sequencing was done using a self-customized targeted

(23) PARP1 Inhibitor and Trabectedin Combination Does Not Increase Tumor Mutational Burden in Advanced Sarcomas-A Preclinical and Translational StudyCancersDecember 15, 2021Pignochino Y, Crisafulli G, Giordano G, Merlini A, Berrino E, Centomo ML, Chiabotto G, Brusco S, Basiricò M, Maldi E, et al.and surgical excision, after microdissection of the tumor area, using the Maxwell® RSC DNA FFPE Kit AS1450 (Promega), and the RSC Maxwell Instrument (Promega) in accordance with the manufacturer’s instructions.

(24) A phase 1/2 trial of an immune-modulatory vaccine against IDO/PD-L1 in combination with nivolumab in metastatic melanomaNature medicineDecember 1, 2021Kjeldsen JW, Lorentzen CL, Martinenaite E, Ellebaek E, Donia M, Holmstroem RB, Klausen TW, Madsen CO, Ahmed SM, Weis-Banke SE, et al.(Qiagen, 565304), and DNA from PFFE biopsies was extracted using the Maxwell RSC DNA FFPE kit (Promega, AS1450). Immunosequencing of the CDR3 regions of human TCRβ chains was performed using the immunoSEQÒ Assay

(25) Experience of Uncommon EGFR Mutation in Lung Scheme Quality Program: Discussing Risks and Opportunities for the Improvement of Laboratory ResponseGlobal Medical GeneticsDecember 1, 2021Alessandro Pancrazzi, Agostino Ognibene, Alice Moncada, Valerio Torreassessed as suitable for molecular analysis. The tissue extraction was performed by Promega FFPE DNA kit (AS1450), and the DNA quantity was measured by Nanodrop Instrument. Extracted DNA amounted to 275 ng/microliter.

(26) Reflex testing in non-small cell lung carcinoma using DNA- and RNA-based next-generation sequencing—a single-center experienceTranslational Lung Cancer ResearchDecember 1, 2021Martin Zacharias et al.performed using the Maxwell 16 instrument (Promega) and the Maxwell RSC DNA FFPE Kit (Promega, CatNr: AS1450). DNA was quantified by Picogreen fluorescence, and 10ng DNA was used for library preparation. NGS libraries

(27) Clinical Value of NGS Genomic Studies for Clinical Management of Pediatric and Young Adult Bone SarcomasCancers (Basel)October 29, 2021Miriam Gutiérrez-Jimeno et al.formalin-fixed paraffin-embedded (FFPE) fresh or frozen tissue using a Maxwell® RSC DNA FFPE kit (Promega, AS1450, Madison, WI, USA) for DNA and a Maxwell® RSC RNA FFPE kit (Promega, AS1440) for RNA. The amount of

(28) Genomic Sub-Classification of Ovarian Clear Cell Carcinoma Revealed by Distinct Mutational SignaturesCancers (Basel)October 19, 2021Douglas V. N. P. Oliveira, Tine H. Schnack, Tim S. Poulsen, Anne P. Christiansen, Claus K. Høgdall, Estrid V. Høgdallextracted by using Maxwell RSC DNA FFPE kit (Promega, Madison, WI, USA), automatized in Maxwell RSC (AS1450) instrument, following manufacturer’s instructions. Briefly, FFPE samples were pre-treated with mineral

(29) Diagnostic Utility of a Custom 34-Gene Anchored Multiplex PCR-Based Next-Generation Sequencing Fusion Panel for the Diagnosis of Bone and Soft Tissue Neoplasms With Identification of Novel USP6 Fusion Partners in Aneurysmal Bone CystsArchives of Pathology & Laboratory MedicineJuly 1, 2021Josephine K. Dermawan et al.Total nucleic acid extraction was performed by using the Maxwell RSC FFPE Kits (catalog Nos. AS1440 and AS1450, Promega) and protocol on the Maxwell RSC instrument. Total nucleic acid was quantified by using both

(30) Detection of PIK3CA Gene Mutation in Head and Neck Squamous Cell Carcinoma Using Droplet Digital PCR and RT-qPCRBiomoleculesMay 31, 2021Edyta M. Borkowska, Magda Barańska, Magdalena Kowalczyk, Wioletta Pietruszewskaisolated from samples using Maxwell RSC DNA FFPE Kit (Promega Corporations, Madison, WI, USA, cat No AS1450) and automatic isolation on AS4500 device (Promega Corporations, Madison, WI, USA). The purity of extracted

(31) Pathology of COVID-19-associated acute kidney injuryClinical kidney journalMarch 1, 2021Sharma P, Ng JH, Bijol V, Jhaveri KD, Wanchoo R.on Dako Omnis (Agilent Technologies, Santa Clara, CA, USA) and Maxwell RSC DNA FFPE Kit (reference: AS1450). Promega Corporation, Madison, WI, USA","The RNAscope® 2.5 HD Duplex Assay (Advanced Cell Diagnostics,

(32) Unspecific post-mortem findings despite multiorgan viral spread in COVID-19 patientsCrit CareAugust 12, 2020Remmelink M, De Mendonça R, D'Haene N, De Clercq S, Verocq C, Lebrun L, Lavis P, Racu ML, Trépant AL, Maris C, et al.rRT-PCR Total nucleic acid was extracted from FFPE tissues using the Maxwell RSC DNA FFPE Kit (reference: AS1450. Promega Corporation, Madison, WI, USA) and the Promega Maxwell extractor, following the protocol described

(33) Identification of a periodontal pathogen and bihormonal cells in pancreatic islets of humans and a mouse model of periodontitisScientific reportsJune 19, 2020Ilievski V, Toth PT, Valyi-Nagy K, Valyi-Nagy T, Green SJ, Marattil RS, Aljewari HW, Wicksteed B, O'Brien-Simpson NM, Reynolds EC, et al.formalin fixed paraffin embedded (FFPE) mouse pancreatic samples using a MaxwellRSC device and the Maxwell DNA FFPE kit (AS1450, Promega Corporation, Madison, WI) and from human frozen pancreatic samples following homogenization

(34) Angiotensinogen promoter methylation predicts bevacizumab treatment response of patients with recurrent glioblastomaMolecular OncologyMay 1, 2020Thomas Urup et al.(Qiagen, Hilden, Germany; 51304 or 51306) in the training cohort and Maxwell RSC DNA FFPE Kit (Promega; AS1450, Madison, WI, USA) in the validation cohort. DNA extracts were stored at −80 °C. DNA concentration was

(35) Fulvestrant plus capivasertib versus placebo after relapse or progression on an aromatase inhibitor in metastatic, oestrogen receptor-positive breast cancer (FAKTION): a multicentre, randomised, controlled, phase 2 trialThe Lancet OncologyMarch 1, 2020Robert H Jones, Angela Casbard, Margherita Carucci, Catrin Cox, Rachel Butler, Fouad Alchami, Tracie-Ann Madden, Catherine Bale, Pavel Bezecny, Johnathan Joffe, et al.were macrodissected and the DNA extracted using the Promega (Madison, WI) Maxwell RSC DNA FFPE kit (AS1450). Upon arrival of the blood samples (and within 96 h of collection), each tube was spun at 2000 g for

(36) Correlation of HPV16 Gene Status and Gene Expression With Antibody Seropositivity and TIL Status in OPSCCFrontiers in oncologyJanuary 1, 2020von Witzleben A, Currall E, Wood O, Chudley L, Akinyegun O, Thomas J, Bendjama K, Thomas GJ, Friedmann PS, King EV, et al.extracted from FFPE tissue in accordance to the manufacturer’s protocol (Maxwell® RSC DNA FFPE Kit, AS1450, Maxwell® RSC RNA FFPE Kit, AS1440, Promega, Southampton, UK). The concentrations of DNA and RNA were

(37) Pharmacokinetics and safety of erlotinib and its metabolite OSI-420 in infants and children with primary brain tumorsCancer Chemotherapy and PharmacologyOctober 1, 2019Samuel J. Reddick, Olivia Campagne, Jie Huang, Arzu Onar-Thomas, Alberto Broniscer, Amar Gajjar, Clinton F. Stewartquantified from formalin-fixed paraffin-embedded tissues and blood with a Maxwell RSC DNA FFPE kit (#AS1450, Promega, Madison, WI, USA), and Qubit (Thermo Fisher Scientific, Waltham, MA, USA) using standard procedures.

(38) Use of FFPE-derived DNA in next generation sequencing: DNA extraction methodsPLOS ONEApril 11, 2019Samantha J. McDonough, Aditya Bhagwate, Zhifu Sun, Chen Wang, Michael Zschunke, Joshua A. Gorman, Karla J. Kopp, Julie M. CunninghamQIAsymphony DSP DNA Mini Kit,937255,Automated,QS-A QIAGEN QIAcube GeneRead DNA FFPE Kit,180134,Automated,QGR-A Promega Maxwell RSC DNA FFPE Kit,AS1450,Automated,PM-A PerkinElmer Chemagic MSM1 FFPE DNA Kit,CMG-1099,Automated,PEC-A ThermoFisher KingFisher

(39) Risk-adapted therapy for young children with medulloblastoma (SJYC07): therapeutic and molecular outcomes from a multicentre, phase 2 trialThe Lancet OncologyJune 1, 2018Giles W. Robinson et al.from formalin-fixed paraffin-embedded (FFPE) tissues and blood using the Maxwell® RSC DNA FFPE kit (#AS1450, Promega, Madison, WI), according to the manufacturer’s instructions, and quantified using Qubit (Thermo

(40) Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type micePloS oneJanuary 1, 2018Ilievski V, Zuchowska PK, Green SJ, Toth PT, Ragozzino ME, Le K, Aljewari HW, O'Brien-Simpson NM, Reynolds EC, Watanabe K.extracted from tissue samples preserved in FFPE using a Maxwell® RSC device and the Maxwell DNA FFPE kit (AS1450, Promega Corporation, Madison, WI) according to the manufacturer’s instructions. Primers and probes

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Maxwell RSC DNA FFPE Kit

Maxwell RSC DNA FFPE Kit from Promega

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Maxwell RSC DNA FFPE Kit from Promega

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