ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) Prolyl Hydroxylase 3 Knockdown Accelerates VHL-Mutant Kidney Cancer Growth In VivoInternational journal of molecular sciencesJanuary 1, 0001"Zacharias NM, Wang L, Maity T, Li L, Millward SW, Karam JA, Wood CG, Navai N."non-essential amino acids. Cells were grown for 24 h. Instructions for the Promega ROS-Glo™ H2 O2 assay (G8820, Promega, Madison, WI, USA) were followed. For the assay, 20 µL of substrate mix was added to each well

(142) Cellular mechanism of action of 2-nitroimidazoles as hypoxia-selective therapeutic agentsRedox biologyJanuary 1, 0001"Rashed FB, Diaz-Dussan D, Mashayekhi F, Macdonald D, Nation PN, Yang XH, Sokhi S, Stoica AC, El-Saidi H, Ricardo C, et al."Corp., Sumerduck, VA). 2.14 Assessing hydrogen peroxide (H2O2) levels The ROS-Glo™ H2O2 assay kit (G8820, Promega, Madison, WI) was used to analyze the levels of H2O2 as a readout of the reactive oxygen species

(143) Altered zinc homeostasis in a primary cell culture model of the retinal pigment epitheliumFrontiers in nutritionJanuary 1, 0001"Álvarez-Barrios A, Álvarez L, Artime E, García M, Lengyel I, Pereiro R, González-Iglesias H."days after renewal of culture media, using the ROS-Glo™ H2O2 Assay purchased from Promega (Cat. No. G8820). H2O2 diffused from the cells to the culture media was quantified by the luminescent signals produced

(144) Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metforminAgingJanuary 1, 0001"García-Puga M, Saenz-Antoñanzas A, Fernández-Torrón R, Munain AL, Matheu A."1x103 fibroblasts were plated in 96-well plates and grown for 3 days. Afterwards, ROS-Glo H2O2 Assay (G8820, Promega) was performed according to the manufacturer’s instructions. Briefly, a H2O2 substrate reacts

(145) Antioxidants stimulate BACH1-dependent tumor angiogenesisThe Journal of clinical investigationJanuary 1, 0001"Wang T, Dong Y, Huang Z, Zhang G, Zhao Y, Yao H, Hu J, Tüksammel E, Cai H, Liang N, et al."and seeded in white 96-well plates (5,000 cells/well). ROS were measured with the ROS-Glo-H2O2 assay (G8820, Promega). The ratio of GSH/GSSG was determined with the GSH/GSSG-Glo assay (V6611, Promega). Fluorescence

(146) PCNA inhibition enhances the cytotoxicity of β-lapachone in NQO1-Positive cancer cells by augmentation of oxidative stress-induced DNA damageCancer lettersJanuary 1, 0001"Su X, Wang J, Jiang L, Chen Y, Lu T, Mendonca MS, Huang X."during or after treatments using standard chemical assays following manufactures’ protocols. (Promega G8820, Promega G9071, Promega G7572). 2.9. PCNA knockdown by siRNA PCNA-specific siRNA ( CCUUGGCGCUAGUAUUUGAtt,

(147) Mechanisms of metabolic stress induced cell death of human oligodendrocytes: relevance for progressive multiple sclerosisActa neuropathologica communicationsJanuary 1, 0001"Fernandes MGF, Mohammadnia A, Pernin F, Schmitz-Gielsdorf LE, Hodgins C, Cui QL, Yaqubi M, Blain M, Hall J, Dudley R, et al."ATP and H2O2 were measured using Cell Titer-Glo 2.0 (Promega, G9242) and ROS-Glo H2O2 Assay (Promega G8820). Normalization to cell number was calculated using the ratio between the measures of ATP and H2O2 obtained

(148) Influence of 2-Nitroimidazoles in the Response of FaDu Cells to Ionizing Radiation and Hypoxia/Reoxygenation Stress"Antioxidants (Basel, Switzerland)"January 1, 0001"Rashed FB, Kate WD, Fanta M, Wiebe LI, Kumar P, Weinfeld M."used as a readout of reactive oxygen species (ROS) and were analyzed using a ROS-Glo™ H2O2 assay kit (G8820, Promega, Madison, WI, USA). Briefly, FaDu cells seeded in 96-well plates were treated as indicated

(149) "Manganese porphyrin, MnTE-2-PyP, treatment protects the prostate from radiation-induced fibrosis (RIF) by activating the NRF2 signaling pathway and enhancing SOD2 and sirtuin activity"Free radical biology & medicineJanuary 1, 0001"Shrishrimal S, Chatterjee A, Kosmacek EA, Davis PJ, McDonald JT, Oberley-Deegan RE."ROS-GLO H2O2 assay Hydrogen peroxide (H2O2) generated by MnTE-2-PyP was measured using the ROS-GLO H2O2 assay (G8820, Promega) as previously described [138]. Briefly, HPrF cells (1 × 104 cells/well in 70 μl of HPrF media)

(150) Cigarette Smoke Extract Activates Hypoxia-Inducible Factors in a Reactive Oxygen Species-Dependent Manner in Stroma Cells from Human Endometrium"Antioxidants (Basel, Switzerland)"January 1, 0001"Kida N, Matsuo Y, Hashimoto Y, Nishi K, Tsuzuki-Nakao T, Bono H, Maruyama T, Hirota K, Okada H."with gDNA Remover TOYOBO FSQ-301 RNeasy Mini Kit Qiagen 74106 ROS-Glo™ H2 O2 Assay Promega G8820 Running Buffer Solution fro SDS-PAGE Nacalai Tesque 30329-61 Tris Bufferd Saline with Tween® 20

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