F-actin Staining Kit - Green Fluorescence - Cytopainter ab112125 from Abcam

Product Specs
ItemF-actin Staining Kit - Green Fluorescence - Cytopainter
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112125
Quantity500 Test
Sample TypeTissue, Adherent cells, Suspension cells
TypeStain
Research AreaResearch Tools
NCBI Full Gene Nameactin beta
NCBI Gene AliasesBRWS1, PS1TP5BP1

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Website: http://www.abcam.com

(1) Fibroblast Growth Factor Receptor Signaling in Maturing Osteoblasts Controls Cell-Matrix Interactions Critical for Osteocyte SurvivalbioRxivMay 5, 2026Debabrata Patra, Craig Smith, Chenming Wei, Courtney M. Mazur, Ilemona Ameadaji, Tiandao Li, Marc N. Wein, Mathew J. Silva, David M. Ornitzwere fixed in 4% PFA, permeabilized in 0.05% saponin, and stained with phalloidin (Abcam Cytopainter, ab112125) and DAPI to visualize the F-actin cytoskeleton. Cells were imaged on a Nikon Eclipse microscope with

(2) The effect of cuproptosis-related gene Atox1 on cardiac hypertrophy in heart failure was investigated based on bioinformatics analysisSci RepDecember 29, 2025Hui Zhou, Ming Yang, Hongfeng Jin, Ziqing Ye0.1% Triton-X for 5 min, cells were first incubated with a monoclonal antibody against F-actin (Abcam, ab112125) overnight at 4 ℃ and subsequently with secondary antibodies for 30 min at room temperature. Thereafter,

(3) Engineering CCR2/IFN-γ overexpression in enucleated mesenchymal stem cells enhances therapy for rheumatoid arthritisCommunications BiologyNovember 27, 2025Yue Sui, Zixi Pu, Naixue Yang, Yifei Luo, Yalian Sa, Qi Qi, Li Tan, Yaping Yan, Wei Siefficiency was assessed using an epifluorescence microscope (Nikon AX/R) after staining with F-actin (Abcam, ab112125) for 30 min. Cellular internal morphology and organelles were visualized using a transmission electron microscopy

(4) Echinococcus granulosus promotes MAPK pathway-mediated osteoclast differentiation by inhibiting Nrf2 in osseous echinococcosisVet ResApril 12, 2025Yaqing Liu, Jing Li, Zhendong Zhang, Qi Li, Yanhu Tian, Sibo Wang, Chenhui Shi, Haohao Sun1:1000) were acquired from Cell Signaling Technology (Massachusetts, USA). An F-actin staining kit (#ab112125) and a ROS content detection kit (#ab113851) were purchased from Abcam. TRAP staining kits were obtained

(5) Local soft niches in mechanically heterogeneous primary tumors promote brain metastasis via mechanotransduction-mediated HDAC3 activitySci AdvFebruary 28, 2025Kai Tang, Yufan Zheng, Guanshuo Hu, Ying Xin, Keming Li, Cunyu Zhang, Xi Chen, Bai Zhang, Xueyi Li, Bing Hu, et al.TCS SPE) using 40× and 63× objectives. For F-actin staining, the CytoPainter F-actin labeling kit (Abcam, #ab112125) was used as per the user guidebook. Images were taken using the confocal microscope or the inverted

(6) ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathwayFront PharmacolFebruary 3, 2025Jing-Yu Hou, Hao Wu, Shuang-Mei Li, Xiao-Jing Li, Shu-Jun Yang, Xu-Xiang Chen, Chang-Qing Zhou, Hui-Bao Long, Hai-Dong Wu, Jia-Ying Fu, et al.used to determine polymerized F-actin using the F-actin staining kit-Green Fluorescence-Cytopainter (#ab112125, Abcam, US). BMSCs were fixed with 4% paraformaldehyde for 30 min followed by permeabilization with

(7) Echinococcus granulosus sensu lato promotes osteoclast differentiation through DUSP4-MAPK signaling in osseous echinococcosisFrontiers in microbiologyJanuary 1, 2025Sun H, Liu Y, Huang Y, Xiong K, Zhang Z, Wang W, Dai Y, Li J, Li Q, Wang S, et al.wash, cells were permeabilized with 0.1% Triton X-100. Cells were stained with the F-actin kit (Abcam, #ab112125) according to the manufacturer’s instructions. F-actins were visualized as green rings under a fluorescence microscope

(8) Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatmentMaterials Today BioOctober 1, 2024Arjun Sabu, Yu Ching Huang, Ramalingam Sharmila, Chih-Yuan Sun, Min-Ying Shen, Hsin-Cheng ChiuFluor® 647 (ab150171) and Goat Anti-Mouse antibody Alexa Fluor® 647 (ab150115) and F-actin staining kit (ab112125) were purchased from Abcam. ECL Substrate kit (APL-1023) was purchased from APOLO Biochemical. Anti-human

(9) Actin Dysregulation Mediates Nephrotoxicity of Cassiae Semen Aqueous ExtractsToxicsJuly 30, 2024Jinlan Yang, Sheng Xiao, Ludi Li, An Zhu, Wusheng Xiao, Qi WangAb2480), anti-cofilin (phospho S3; Cat. # ab 12866), and F-actin staining kit (green fluorescence; Cat. # ab112125) were purchased from Abcam (Cambridge, UK). Anti-RhoA (26C4) (Cat. # sc-418) and anti-GAPDH (Cat. #

(10) DNA-PKcs phosphorylates cofilin2 to induce endothelial dysfunction and microcirculatory disorder in endotoxemic cardiomyopathyResearch (Wash D C)March 26, 2024Yingzhen Du, Pingjun Zhu, Yukun Li, Jiachi Yu, Tian Xia, Xing Chang, Hang Zhu, Ruibing Li, Qingyong HeFollowing a 1-h block in 10% goat serum at RT, slides were incubated with antibodies targeting F-actin (#ab112125, Abcam), VE-cadherin (#ab205336, Abcam), Gr-1 (#ab314120, Abcam), TnT (#ab8295, Abcam), α-SMA (#ab5831,

(11) MELAS-Derived Neurons Functionally Improve by Mitochondrial Transfer from Highly Purified Mesenchymal Stem Cells (REC)Int J Mol SciDecember 6, 2023Lu Liu, Jiahao Yang, Yoshinori Otani, Takahiro Shiga, Akihiro Yamaguchi, Yasuaki Oda, Miho Hattori, Tsukimi Goto, Shuichi Ishibashi, Yuki Kawashima-Sonoyama, et al.by staining the fixed cells with 1X Green Fluorescent Phalloidin Conjugate working solution (Abcam, ab112125, Cambridge, UK) for 45 min at RT. After gentle washing twice with PBS, the cells were visualized under

(12) The mechanosensitive ion channel Piezo1 modulates the migration and immune response of microgliaiScienceFebruary 17, 2023Zhu T, Guo J, Wu Y, Lei T, Zhu J, Chen H, Kala S, Wong KF, Cheung CP, Huang X, et al.and store at 4°C overnight. Actin polymerization staining Green fluorescent phalloidin conjugate (ab112125, Abcam) was used to stain F-actin according to the manufacturer’s recommendation. Cells were then washed

(13) Partial prevention of glucocorticoid-induced osteocyte deterioration in young male mice with osteocrin gene therapyiScienceSeptember 16, 2022Courtney M. Mazur, Christian D. Castro Andrade, Nicha Tokavanich, Tadatoshi Sato, Michael Bruce, Daniel J. Brooks, Mary L. Bouxsein, Jialiang S. Wang, Marc N. Weinkit, fluorescein (TUNEL)",Roche,Cat#11684795910 CytoPainter F-actin staining kit - Green,Abcam,Cat#ab112125 PureLink RNA Mini Kit,Invitrogen,Cat#12183025 PerfeCT SYBR Green FastMix,Quantabio,Cat#95073 PrimeScript

(14) Extracellular Vesicles from Naegleria fowleri Induce IL-8 Response in THP-1 MacrophagePathogens (Basel, Switzerland)May 30, 2022Lertjuthaporn S, Somkird J, Lekmanee K, Atipimonpat A, Sukapirom K, Sawasdipokin H, Tiewcharoen S, Pattanapanyasat K, Khowawisetsut L.Fluorescent Phalloidin Conjugate working solution using F-actin Staining Kit (Cytopainter, Cat. no. ab112125, Abcam, Cambridge, MA, USA) at 37 °C, 5% CO2 for 1 h. Next, the stained cells were washed with PBS.

(15) Fluid shear stress regulates the survival of circulating tumor cells via nuclear expansionJournal of Cell ScienceMay 15, 2022Zichen Xu, Keming Li, Ying Xin, Kai Tang, Mo Yang, Guixue Wang, Youhua Tanwith 0.1% Triton X-100 for 5 min, and blocked with 1% BSA for 1 h. Cells were stained for F-actin (ab112125, Abcam) and with DAPI (11569306, Invitrogen, USA) for 1 h at room temperature. After being washed three

(16) Evasion of NK cell immune surveillance via the vimentin-mediated cytoskeleton remodelingFrontiers in immunologyJanuary 1, 2022Peng JM, Chiu CF, Cheng JH, Liu HY, Chang YL, Luo JW, Weng YT, Luo HL.USA), vimentin was from abcam (ab92547, Cambridge, UK). Phalloidin staining reagent was from Abcam (ab112125, Cambridge, UK). Natural killer cell cytotoxicity assay Cells were seeded at 2 x 104 or 4 x 104 cells

(17) VIP Stabilizes the Cytoskeleton of Schlemm's Canal Endothelia via Reducing Caspase-3 Mediated ZO-1 Endolysosomal DegradationOxidative medicine and cellular longevityJanuary 1, 2021Lou X, Mou Q, Zhao B, Huang J, Yao K, Luo Z, Ye M, Hu Y, Duan Q, Li X, et al.(Proteintech, 60004-1-Ig), anti-β-actin (Proteintech, 60008-1-Ig), F-actin Staining Kit (Abcam, ab112127 and ab112125), and Fluorescein isothiocyanate-dextran (Sigma, FD70S). 2.3. Cell Culture and Transfection Human

(18) Expression of ILK in renal stroma is essential for multiple aspects of renal developmentAmerican Journal of Physiology-Renal PhysiologyAugust 1, 2018Xiaohui Gong et al.and incubated with primary antibodies overnight at 4°C. The following primary antibodies were used: F-actin (ab112125, Abcam) and Paxillin (ab32115, Abcam). After washing in 0.25% TritonX-100 in PBS, cells were incubated

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F-actin Staining Kit - Green Fluorescence - Cytopainter from Abcam

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F-actin Staining Kit - Green Fluorescence - Cytopainter from Abcam

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