TMB ELISA Substrate (Highest Sensitivity) ab171522 from Abcam

Product Specs
ItemTMB ELISA Substrate (Highest Sensitivity)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab171522
Quantity100 mL, 1000 mL
ApplicationsELISA

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cellsNat MetabApril 3, 2026Mu A, Gang Wang, Nathan W. Zammit, Laura Roth, Yasheng Maierhaba, Dina Bogoslavski, Chufan Cai, P. Kent Langston, Qiuyang Zhang, Inna S. Afonina, et al.peroxidase-linked secondary antibody for 30 min following 4× PBST wash. 3,3’,5,5’-Tetramethylbenzidine chromogen (ab171522, Abcam) was used as a detection system and, finally, the absorbance was measured at 450 nm after adding

(2) Protocol to set up the immuno-microfluidic system for rapid measurement of salivary cortisol levelSTAR ProtocolsDecember 1, 2025Yecan Wang, Kaito Maehara, Shigenobu Mitsuzawa, Ryo Miyake, Madoka Takaipellets,PS Japan Co. Ltd.,SGP-10 Bovine serum albumin,Sigma-Aldrich,1002586561 Tetramethylbenzidine,Abcam,ab171522 "Anti-cortisol, XM-210",GeneTex,RRID: AB_380320 Salivary cortisol ELISA kit,Yanaihara Institute Inc.,YK241 Potassium

(3) Topical application of the HSP90 inhibitor 17-AAG reduces skin inflammation and partially restores microbial balance: implications for atopic dermatitis therapySci RepJuly 1, 2025Krzysztof Sitko, Ewa Piotrowska, Magdalena Podlacha, Natalia Zagórska, Michał D. Starke, Magdalena Trzeciak, Stefan Tukaj(ab205718, Abcam) were added and incubated for 1 h at RT. After washing, the substrate solution TMB (ab171522, Abcam) was added, and the reaction was stopped after 20 min with 0.5 M H₂SO₄. The absorbance was measured

(4) X-Ray Exposure Induces Structural Changes in Human Breast ProteinsInt J Mol SciJune 13, 2025Ren Jie Tuieng, Sarah H. Cartmell, Cliona C. Kirwan, Alexander Eckersley, Michael J. Sherrattwash with TBST, 100 μL of 3,3′,5,5′-tetramethylbenzidine (TMB) ELISA substrate (Highest sensitivity) (ab171522, Abcam, Cambridge, UK) was added per well and incubated for 40 min. The reaction was stopped by adding

(5) Targets for the diagnosis of Acanthamoeba eye infections include four cyst wall proteins and the mannose-binding domain of the trophozoite mannose-binding proteinmSphereMarch 25, 2025Bharath Kanakapura Sundararaj, Manish Goyal, John Samuelsonsecondary antibody diluted 1:6,000. Plates were washed and then developed using TMB ELISA substrate (Abcam, Ab171522), and the absorbance was recorded at 370 nm. The OD of each sample dilution was calculated as the OD

(6) A Targetable Secreted Neural Protein Drives Pancreatic Cancer Metastatic Colonization and HIF1α Nuclear RetentionCancer DiscoveryDecember 2, 2024Norihiro Yamaguchi, Y. Gloria Wu, Ethan Ravetch, Mai Takahashi, Abdul G. Khan, Akimasa Hayashi, Wenbin Mei, Dennis Hsu, Shigeaki Umeda, Elisa de Stanchina, et al.plate was incubated for 40min at 37°C. Following to an iterative wash with PBS, TMB substrate (Abcam, #ab171522) was added to each well and the plate was incubated at 37°C for 10–30min. Following to the addition

(7) A nonadjuvanted HLA-restricted peptide vaccine induced both T and B cell immunity against SARS-CoV-2 spike proteinScientific ReportsSeptember 4, 2024Yi-Ru Lee, Chiung-Wen Liou, I-Hua Liu, Jia-Ming Changwith anti-mouse HRP antibody, the reaction microplate was washed four times, 100 μL of TMB Solution (ab171522, Abcam, Cambridge, UK) was added, and the plate was incubated at RT for 20 min. Finally, 100 μL of Stop

(8) Altered Elastin Turnover, Immune Response, and Age-Related Retinal Thinning in a Transgenic Mouse Model With RPE-Specific HTRA1 OverexpressionInvestigative ophthalmology & visual scienceJuly 1, 2024Navneet S, Ishii M, Rohrer B.(Cell Signaling, 3999S) at 1:2000 dilution, followed by color development with TMB substrate (Abcam, ab171522). The absorbance was measured at 450 nm after stopping the reaction with STOP solution (Abcam, ab171529). Detection

(9) Surface Acoustic Wave-Driven Enhancement of Enzyme-Linked Immunosorbent Assays: ELISAWAnalytical ChemistryJune 11, 2024Lei Zhang, Shuai Zhang, Cécile Floer, Sreeya Anjana Raj Kantubuktha, María José González Ruiz Velasco, James Friendthe SAW at 40, 60, or 100 MHz for 15 min, before finally adding TMB (3,3′,5,5′ tetramethylbenzidine, ab171522, Abcam) to incubate for 5 min (with or without SAW). Plotted in Figure 2, the absorbance intensity with

(10) Lung-specific interleukin 6 mediated transglutaminase 2 activation and cardiopulmonary fibrogenesisFrontiers in ImmunologyApril 8, 2024Krishna C. Penumatsa, Yamini Sharma, Rod R. Warburton, Adit Singhal, Deniz Toksoz, Chinmayee D. Bhedi, Guanming Qi, Ioana R. Preston, Christina Anderlind, Nicholas S. Hill, et al.Cells were then incubated with a chromogenic substrate and visualizing reagent, TMB solution (Cat# ab171522; Abcam). After 5 minutes, Stop Solution for TMB (Cat# ab171529; Abcam) was added to each well and the

(11) An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisoliScienceOctober 20, 2023Wang Y, Murakami H, Kasama T, Mitsuzawa S, Shinkawa S, Miyake R, Takai M.50-24-8 Progesterone,FUJIFILM Corp.,CAS RN®: 57-83-0 "3,3′,5,5′-tetramethylbenzidine (TMB) substrate",Abcam Co.,ab171522 "Mucin, from porcine stomach",FUJIFILM Corp.,CAS RN®: 84082-64-4 ,, Critical commercial assays,Critical

(12) Structural basis for antibody-mediated neutralization of lymphocytic choriomeningitis virusCell chemical biologyApril 20, 2023Moon-Walker A, Zhang Z, Zyla DS, Buck TK, Li H, Diaz Avalos R, Schendel SL, Hastie KM, Crotty S, Saphire EO.were once again washed six times with ELISA buffer. TMB ELISA Substrate (Highest Sensitivity) (Abcam ab171522) was added, followed by equal volumes of 650 nm Stop Solution for TMB Substrate (Abcam ab171531). The

(13) Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challengeBiomaterialsJanuary 1, 2023Sun S, Li E, Zhao G, Tang J, Zuo Q, Cai L, Xu C, Sui C, Ou Y, Liu C, et al.incubated for 40 min at 37 °C. Plates were then washed three-times and added with peroxidase substrate (Ab171522, Abcam, UK), the reaction was terminated by stop solution (Ab171529, Abcam, UK) and the absorbance at

(14) Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responsesProceedings of the National Academy of Sciences of the United States of AmericaAugust 9, 2022Wang Y, Feswick A, Apostolou V, Petkov PM, Moser EK, Tibbetts SA.1091-05; IgG2c: 1078-05; and IgG3: 1101-05). After a final three washes, TMB ELISA Substrate (Abcam, ab171522) was added to detect HRP enzymatic activity. The reactions were then quenched by the addition of Stop

(15) Stability of SARS-CoV-2-Encoded Proteins and Their Antibody Levels Correlate with Interleukin 6 in COVID-19 PatientsmSystemsJune 28, 2022Li W, Kitsios GD, Bain W, Wang C, Li T, Fanning KV, Deshpande R, Qin X, Morris A, Lee JS, et al.ab76949) and 3,3′,5,5′-tetramethylbenzidine (TMB) ELISA substrate (highest sensitivity) (catalog number ab171522) were purchased from Abcam (Cambridge, MA). A goat anti-mouse IgG-horseradish peroxidase (HRP) conjugate

(16) CD164 is a host factor for lymphocytic choriomeningitis virus entryProceedings of the National Academy of Sciences of the United States of AmericaMarch 8, 2022Bakkers MJG, Moon-Walker A, Herlo R, Brusic V, Stubbs SH, Hastie KM, Saphire EO, Kirchhausen TL, Whelan SPJ.respective pH. TMB (3,3′,5,5′-tetramethylbenzidine) ELISA Substrate (Highest Sensitivity) (Abcam No. ab171522) was added, followed by equal volumes of 650 nm Stop Solution for TMB Substrate (Abcam No. ab171531).

(17) Fortilin interacts with TGF-β1 and prevents TGF-β receptor activationCommunications biologyFebruary 23, 2022Pinkaew D, Martinez-Hackert E, Jia W, King MD, Miao F, Enger NR, Silakit R, Ramana K, Chen SY, Fujise K.gentle shaking. Finally, after extensive washing, we added TMB substrate solution (Abcam, Catalog #: ab171522) to each well, incubated the plate in the dark for 3 min at room temperature with gentle shaking, added

(18) Exercise hormone irisin is a critical regulator of cognitive functionNature MetabolismAugust 20, 2021Mohammad R. Islam, Sophia Valaris, Michael F. Young, Erin B. Haley, Renhao Luo, Sabrina F. Bond, Sofia Mazuera, Robert R. Kitchen, Barbara J. Caldarone, Luis E. B. Bettio, et al.secondary antibody for 30 min following 4X PBST wash. 3,3’,5,5’-Tetramethylbenzidine (TMB) chromogen (ab171522, Abcam) was used as detection systems, and finally, the absorbance was measured at 450nm after adding

(19) Systemic Treatment of Fabry Disease Using a Novel AAV9 Vector Expressing Î±-Galactosidase AMolecular Therapy - Methods & Clinical DevelopmentMarch 12, 2021Maria Grazia Biferi, Mathilde Cohen-Tannoudji, Andrea GarcÃa-Silva, Olga Souto-RodrÃguez, Irene ViÃ©itez-GonzÃ¡lez, Beatriz San-MillÃ¡n-Tejado, Andrea FernÃ¡ndez-Carrera, Tania PÃ©rez-MÃ¡rquez, Susana Teijeira-Bautista, Soraya Barrera, et al.Samples were incubated 1 h at room temperature and then visualized using the BioFX TMB/Stop solution (#ab171522; Abcam). The reaction was halted with 1 vol of HCl 0.1 N. Absorbance was measured at 450 nm. IgG concentrations

(20) Bamboo Shark as a Small Animal Model for Single Domain Antibody ProductionFrontiers in bioengineering and biotechnologyJanuary 1, 2021Wei L, Wang M, Xiang H, Jiang Y, Gong J, Su D, Al Azad MAR, Dong H, Feng L, Wu J, et al.secondary antibody and then incubated at RM for 1 h. ELISA was developed with TMB substrate (Abcam, ab171522) and then read at 450 nm. Three shark individuals in each immunization program were involved in the

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TMB ELISA Substrate (Highest Sensitivity) from Abcam

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TMB ELISA Substrate (Highest Sensitivity) from Abcam

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