Propidium Iodide ab14083 from Abcam

Product Specs
ItemPropidium Iodide
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab14083
Quantity1 mL
Concentration1 mg/ml
ApplicationsFlow Cyt, Fluorescence Microscopy

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

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Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Acidic pH Restricts Non‐Tuberculous Mycobacteria ReplicationMolecular MicrobiologyMay 1, 2026Parise K. Lockwood, Luiz Pedro Sorio de Carvalho, Rachel P. J. Lai, Maximiliano G. Gutierrezdeath, the growth media was replaced with RPMI 1640 containing 0.4 μg/mL propidium iodide (PI; Abcam, ab14083), 100 nM BafA1 (Sigma‐Aldrich B1793) or 15 mM of NH4Cl (Sigma‐Aldrich A9434) where stated. For co‐localisation

(2) Investigating PKD2 deficiency-associated cardiomyopathies using hESC-cardiomyocytes and bioengineered 3D ventricular cardiac tissue stripsCell Death & DiseaseMarch 25, 2026Jingxuan Li, Wentao Peng, Maxwell Kwok, Huanyu Ding, Duan Zhuo, Bimal Gurung, Ishan Raj Lakhani, Hongyan Yu, Ellen N. Poon, Ronald A. Li, et al.apoptotic cells. Briefly, cells were incubated with APC-Annexin V (550474, BD, USA) or propidium iodide—PI (ab14083, Abcam, USA) for 30 min in the dark. Flow cytometry was performed by BD FACSymphony A5.2 SORP Flow Cell

(3) MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosisNeoplasiaMarch 1, 2026Bishi Wang, Kongxiu Wu, Xin Liu, Yuhai Shen, Yanliang Lihours. Cells were resuspended in 500 μl of phosphate buffer saline, treated with Propidium Iodide (#ab14083; Abcam) for 30 minutes, and then subjected to flow cytometry using the Attune™ NxT Flow Cytometer (Thermo Fisher Scientific

(4) Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activationNature GeneticsFebruary 16, 2026Tessa M. Popay, Ami Pant, Femke Munting, Melodi Tastemel, Morgan E. Black, Nicholas Haghani, Jesse R. Dixoncells were then resuspended in propidium iodide staining buffer (10 μg/ml propidium iodide (Abcam #ab14083), 100 μg/ml RNase (Thermo Scientific #EN0531), 2 mM MgCl2, DPBS) and incubated overnight at 4°C. Flow cytometry analysis

(5) Aeromonas spp. as a fast-growing high-performance chassis for protein productionApplied and Environmental MicrobiologyJuly 23, 2025Ming-Xuan Tang, Peng-Fei Meng, Ruo-Lin Huang, Xin Zheng, Chen-Chen Liang, Xuepiao Pu, Chen Wang, Ying Zhao, Yi-Qiu Zhang, Jia-Xin Liang, et al.gentamicin to inhibit the growth of extracellular bacteria. Subsequently, propidium iodide (PI) (Abcam, ab14083) was added to the medium, and cell viability was monitored every hour using the IncuCyte system over

(6) Nanosilicates promote angiogenesis through activation of ROS-mediated WNT/β-catenin pathwaySci AdvJuly 4, 2025Giriraj Lokhande, Kanwar Abhay Singh, Shounak Roy, John Soukar, Anna Kersey, Pranita Borkar, Saptarshi Biswas, Raza Ur Rehman Syed, Anna Keller, Kayla J. Bayless, et al.70% ethanol. Fixed cells were stained with 200 μl of propidium iodide (40 μg/ml; Abcam, catalog no. ab14083) for 30 min at 37°C and analyzed for fluorescence at 617 nm using flow cytometry with the BD Accuri

(7) TNIK-driven regulation of ERK5 transcriptional activity in endothelial cellsFront Cardiovasc MedJuly 2, 2025Venkata Subrahmanya Kumar Samanthapudi, Sivareddy Kotla, Nhat-Tu LeV Binding Buffer (#ab14084; Abcam) to prepare baseline controls (unstained cells). Annexin V-FITC (#ab14083; Abcam) was added, and samples were incubated for 5 min at room temperature in the dark. Stained cells

(8) Targeting bladder cancer: Potent anti-cancer effects of cannabichromene and delta-9-tetrahydrocannabinol-rich Cannabis sativa strainsAsian Journal of UrologyApril 9, 2025Omer Anis, Vered Bar, Adi Zundelevich, Seegehali M. Anil, Yaron Shav-Tal, Amos Toren, Dan Dominissini, Gil Raviv, Menachem Laufer, Alon Lazarovich, et al.apoptosis assay, a MEBCYTO® Apoptosis Kit with Annexin V-FITC (Milan, Italy) and propidium iodide (PI, ab14083, Abcam, Cambridge, UK) was used. Cells were seeded in 6-well plates, at a density of 5×105 cells/well.

(9) Mycobacterium tuberculosis phagosome Ca(2+) leakage triggers multimembrane ATG8/LC3 lipidation to restrict damage in human macrophagesScience advancesMarch 28, 2025Chen D, Fearns A, Gutierrez MG.uptake, cells were washed with PBS and replaced with growth medium containing PI (0.4 μg/ml; Abcam, ab14083). Imaging was performed using an OPERA Phenix microscope (PerkinElmer) with a 40× 1.1-NA water-immersion

(10) Influence of Injectable Hyaluronic Gel System on Skin Microbiota, Skin Defense Mechanisms and Integrity (Ex vivo Study)Clinical, Cosmetic and Investigational DermatologyMarch 1, 2025Aleksei Y Prokopov, Zarema I Gazitaeva, Anna N Sidorina, Laurent Peno-Mazzarino, Nikita Radionov, Anna O Drobintseva, Igor M KvetnoyAntibody, Alexa Fluor™ 488 (1:1000, Abcam) were used. Constraining of nucleus with Propidium Iodide (Abcam, ab14083). Immunostaining was assessed by microscopic observation and image analysis, and expressed as percentage

(11) Influence of Injectable Hyaluronic Gel System on Skin Microbiota, Skin Defense Mechanisms and Integrity (Ex vivo Study)Clin Cosmet Investig DermatolMarch 1, 2025Aleksei Y Prokopov, Zarema I Gazitaeva, Anna N Sidorina, Laurent Peno-Mazzarino, Nikita Radionov, Anna O Drobintseva, Igor M KvetnoyAntibody, Alexa Fluor™ 488 (1:1000, Abcam) were used. Constraining of nucleus with Propidium Iodide (Abcam, ab14083). Immunostaining was assessed by microscopic observation and image analysis, and expressed as percentage

(12) The Deubiquitinase OTUD1 Influences HIV-1 Release by Regulating the Host Restriction Factor BST-2VirusesFebruary 14, 2025Man-Di Zhang, Fan Chen, Wen-Qiang He, Ying Lu, Feng-Liang Liu, Hong-Guang Zhang, Liu-Meng Yang, Chun-Sheng Dong, Si-Dong Xiong, Yong-Tang Zhengwashed twice with pre-cooled PBS, and then labeled with phycoerythrin–propidium iodide (PE-PI, Abcam, ab14083, Cambridge, UK) and APC anti-human BST-2 (BioLegend, 348402, San Diego, CA, USA) antibodies at room

(13) Therapeutic potential of brentuximab vedotin in breast cancer and lymphoma via targeted apoptosis and gene regulationSci RepJanuary 13, 2025Abeer Ezzat, Mohga Shafiek, Shimaa Shawki, Shaimaa Abdel-Ghany, Mahmoud Nazih, Hussein SabitBaltics UAB, USA), HERAPLUS SYBR® Green qPCR Kit (Willowfort, Nottingham, UK), and propidium iodide (PI, ab14083, Abcam) were employed. Cell lines The breast cancer cell lines utilized in this study included triple-negative

(14) Polyphyllin VII enhances the sensitivity of endometrial carcinoma cells to medroxyprogesterone acetate through upregulating miR‑33a‑5p expressionOncology LettersNovember 22, 2024Haoen Liu, Yan Peng, Xiaodan Zhuangto remove the ethanol. Subsequently, the cells were stained with propidium iodide (Abcam; cat. no. ab14083) for 30 min at 37°C. Then, flow cytometry (LSRFortessa; BD Biosciences) was performed to analyze the

(15) Lycopene inhibits pyroptosis of endothelial progenitor cells induced by ox-LDL through the AMPK/mTOR/NLRP3 pathwayOpen Med (Wars)June 24, 2024Chujun Tan, Junqiu Chen, Tengcan Tu, Lifang Chen, Jun Zousoftware. 2.8 Flow cytometry Pyroptosis was assessed via flow cytometric analysis using propidium iodide (#ab14083; Abcam, UK), and an active caspase-1 antibody (#89332; Cell Signaling Technology, USA). To identify

(16) miR‐31‐5p suppresses myocardial hypertrophy by targeting Nfatc2ipJournal of Cellular and Molecular MedicineJune 19, 2024Lamei Zhao, Xiaotao Qian, Zhenxing Ren, Ailing Wangstained with 4′,6‐diamidino‐2‐phenylindole (DAPI, 1:1000, D8200, Solarbio) or propidium iodide (PI, 1:50, ab14083, Abcam) combined using RNaseA (1:100, ab300192, Abcam) for 30 min at 37°C. The images were captured

(17) Superparamagnetic Iron Oxide Nanoparticles Reprogram the Tumor Microenvironment and Reduce Lung Cancer Regrowth after Crizotinib TreatmentACS NanoApril 30, 2024Natalie K. Horvat, Sara Chocarro, Oriana Marques, Tobias A. Bauer, Ruiyue Qiu, Alberto Diaz-Jimenez, Barbara Helm, Yuanyuan Chen, Stefan Sawall, Richard Sparla, et al.in ice cold 70% ethanol, washed with PBS and resuspended in a solution of 10 μg/mL propidium iodide (ab14083, Abcam) and 5 μg ribonuclease (12091021, Life Technologies). Mouse lungs were resected and washed in

(18) Integrated transcriptome and cell phenotype analysis suggest involvement of PARP1 cleavage, Hippo/Wnt, TGF-β and MAPK signaling pathways in ovarian cancer cells response to cannabis and PARP1 inhibitor treatmentFrontiers in GeneticsJanuary 23, 2024Nurit Shalev, Michelle Kendall, Navin Kumar, Sudeep Tiwari, Seegehalli M. Anil, Hagit Hauschner, Savvemala G. Swamy, Adi Doron-Faingenboim, Eduard Belausov, Bruce E. Kendall, et al.used to wash twice the fixed cells and then cells were stained with 500 μL of 20 μg/mL PI solution (AB-ab14083, Abcam) containing 50 μg/mL RNase A (EN0531, Thermo Scientific, UDA) for 30 min in the dark. Cell populations

(19) Mechanistic basis for potassium efflux–driven activation of the human NLRP1 inflammasomeProceedings of the National Academy of SciencesJanuary 9, 2024Pritisha Rozario, Miriam Pinilla, Leana Gorse, Anna Constance Vind, Kim S. Robinson, Gee Ann Toh, Muhammad Jasrie Firdaus, José Francisco Martínez, Swat Kim Kerk, Zhewang Lin, et al.were treated with the respective chemicals and stained with 0.5 µg/mL of propidium iodide (PI, Abcam #ab14083) before the plate was loaded into a high-content screening microscope (Perkin Elmer Operetta CLS imaging

(20) Enhanced therapeutic efficacy of doxorubicin/cyclophosphamide in combination with pitavastatin or simvastatin against breast cancer cellsMed OncolDecember 5, 2023Samar A. Dewidar, Omar Hamdy, Moetaza M. Soliman, Amal M. El Gayar, Mohamed El-MeseryWaltham, MA, USA), HERAPLUS SYBR® Green qPCR Kit (Willowfort, Nottingham, UK) and propidium iodide (PI, ab14083, Abcam). Experimental cell lines Breast cancer cell lines (M.D. Anderson - Metastatic Breast 231 (MDA-MB-231)

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Propidium Iodide from Abcam

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