Live and Dead Cell Assay ab115347 from Abcam

Product Specs
ItemLive and Dead Cell Assay
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab115347
Quantity1000 test
ApplicationsCellular Assay
Detection TargetLive dead cell

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human SkinACS NanoNovember 14, 2023Juliana Bolsoni, Danny Liu, Fatemeh Mohabatpour, Ronja Ebner, Gaurav Sadhnani, Belal Tafech, Jerry Leung, Selina Shanta, Kevin An, Tessa Morin, et al.the cytotoxic effect of RNP- and mRNA-loaded LNPs on the viability of KCs, a live dead cell assay (ab115347, Abcam, UK) was performed according to the manufacturer’s instructions. KCs were cultured as described

(2) In need of age‐appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomesAging CellNovember 1, 2023S. Gulberk Ozcebe, Pinar Zorlutunawas profiled (R&D Systems, #ARY009), and survival rate was measured via live/dead staining (Abcam, #ab115347). Aged cells were treated with decellularized ECM for 10 days and control groups were maintained in

(3) Fabrication of Interleukin-4 Encapsulated Bioactive Microdroplets for Regulating Inflammation and Promoting OsteogenesisInternational Journal of NanomedicineApril 19, 2023Yi Zhang, Jin Cao, Minghui Jian, Zhixiao Zhou, Nadia Anwar, Lan Xiao, Yaping Ma, Dingmei Zhang, Jun Zhang, Xin WangLive and dead cell assay was performed using the Live and Dead Cell Assay kit following the manufacturer’s instructions (ab115347, Abcam, China). Briefly, cells were treated with different formulation of MDs. At the end of the treatment,

(4) Monolayer heterojunction interactive hydrogels for high-freedom 4D shape reconfiguration by two-photon polymerizationInternational Journal of BioprintingFebruary 3, 2023Yufeng Tao, Chengchangfeng Lu, Xuejiao Wang, Zhiduo Xin, Xia Cao, Yunpeng Rencytocompatibility. The viability of fibroblasts was examined using the live-dead assay kit (Abcam, ab115347), and the ratios of live/dead cells were calculated with Image J. 2.6. Optical spectroscopic investigation To

(5) Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis modelJournal of orthopaedic translationJanuary 1, 2023Hsueh YH, Buddhakosai W, Le PN, Tu YY, Huang HC, Lu HE, Chen WL, Tu YK.medium was discarded. The cells were rinsed in dPBS once before incubation in 5x Live and Dead Dye (ab115347, Abcam) for 10 min at room temperature with gentle shaking. The number of live and dead cells was determined

(6) A tunable physiomimetic stretch system evaluated with precision cut lung slices and recellularized human lung scaffoldsFrontiers in Bioengineering and BiotechnologyOctober 3, 2022Oskar Rosmark, Arturo IbÃ¡Ã±ez-Fonseca, Johan Thorsson, GÃ¶ran Dellgren, Oskar Hallgren, Anna-Karin Larsson Callerfelt, Linda Elowsson, Gunilla Westergren-Thorssonremoved, and the PCLS were washed in PBS and stained with a Live and Dead Cell Assay solution (Abcam, ab115347; 1:200 in PBS) supplemented with Calcein AM Viability Dye (eBioscience, Thermo Fisher Scientific) at

(7) Combination of Live Cell Surface-Enhanced Raman Scattering Imaging with Chemometrics to Study Intracellular Nanoparticle DynamicsACS SensorsJune 24, 2022Elisa Lenzi, Malou Henriksen-Lacey, Beatriz Molina, Judith Langer, Carlos D. L. de Albuquerque, Dorleta Jimenez de Aberasturi, Luis M. Liz-Marzánequipped with a 785 nm laser. After 24 h, cell viability was monitored using a live/dead cell assay (ab115347, Abcam). For each irradiated section of the grid, the percentage of dead cells was quantified and represented

(8) High throughput, label-free isolation of circulating tumor cell clusters in meshed microwellsNature communicationsJune 13, 2022Boya M, Ozkaya-Ahmadov T, Swain BE, Chu CH, Asmare N, Civelekoglu O, Liu R, Lee D, Tobia S, Biliya S, et al.remaining empty. Measurement of the cell viability To determine the viability of cells, live/dead assay (ab115347, Abcam, Cambridge, MA) was performed according to the manufacturer’s instructions. The assay was mixed

(9) Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial functionBioengineeredMay 26, 2022He Teng et al.to 4-HNE for 24 h and then cultured in the complete medium for 24 h. Live and dead staining agents (ab115347, Abcam) were prepared: 2 µL of ethidium homodimer-1 (EthD-1) and 0.5 µL of calcein Am were added to

(10) Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial CellsInternational Journal of Molecular SciencesApril 29, 2022Francesca Bodega, Chiara Sironi, Luciano Zocchi, Cristina Portarepresentative image of gels before and after µ measurement. Cells were marked with Live and Dead Cell Assay (ab115347, Abcam, Cambridge, UK), which labels live cells in green and dead cells in red. Dead cells did not seem

(11) Synthesis of Poly(acrylic acid)-Cysteine-Based Hydrogels with Highly Customizable Mechanical Properties for Advanced Cell Culture ApplicationsACS OmegaMarch 22, 2022Sharon O. Bolanta, Sigita Malijauskaite, Kieran McGourty, Emmet J. O’ReillyCytotoxicity Cytotoxicity was evaluated after 72 h of cell culture, using a live and dead cell assay kit (ab115347, Abcam). Briefly, media were aspirated from each well and replaced with live and dead cell assay solution

(12) Injectable hydrogel with nucleus pulposus-matched viscoelastic property prevents intervertebral disc degenerationJournal of orthopaedic translationMarch 1, 2022Jia H, Lin X, Wang D, Wang J, Shang Q, He X, Wu K, Zhao B, Peng P, Wang H, et al.pre-treated with hydrostatic or dynamic pathological loading were stained by live-dead assay kit (Abcam, ab115347). The live cell dye labeled intact, viable cells green. It was membrane permeant and non-fluorescent

(13) Metabolic determination of cell fate through selective inheritance of mitochondriaNature cell biologyFebruary 1, 2022Döhla J, Kuuluvainen E, Gebert N, Amaral A, Englund JI, Gopalakrishnan S, Konovalova S, Nieminen AI, Salminen ES, Torregrosa Muñumer R, et al.plated in mammosphere culture or used in fat pad transplantations. Live and Dead Cell Assay (Abcam, ab115347) was used for cell viability studies according to manufacturer’s instructions. Samples were imaged using

(14) Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and CavitationBioengineering (Basel)January 15, 2022Matthew Jorgensen, Pujhitha Ramesh, Miriam Toro, Emily Evans, Nicholas Moskwa, Xulang Zhang, Susan T. Sharfstein, Melinda Larsen, Yubing Xiewas aspirated until 100 µL of medium remained, then 6 µL of Live and Dead Cell Assay stain solution (ab115347, Abcam, Branford, CT, USA) was added to the medium in each well. Additionally, high ratios of epithelial

(15) Egg White Alginate as a Novel Scaffold Biomaterial for 3D Salivary Cell CulturingBiomimetics (Basel)December 28, 2021Hieu M. Pham, Yuli Zhang, Jose G. Munguia-Lopez, Simon D. TranCO2. Live–Dead staining was performed on day 5 and 10 using a Live and Dead Cell Assay kit (Abcam, ab115347). The 4 mM calcein acetoxymethyl (CalAM) and 2mM ethidium homodimer III (EthD-III) stock solutions were

(16) Negative enrichment of circulating tumor cells from unmanipulated whole blood with a 3D printed deviceScientific ReportsDecember 1, 2021Chia-Heng Chu, Ruxiu Liu, Tevhide Ozkaya-Ahmadov, Brandi E. Swain, Mert Boya, Bassel El-Rayes, Mehmet Akce, Mehmet Asim Bilen, Omer Kucuk, A. Fatih Sarioglu103 tumor cells/mL. The viability of enriched tumor cells was measured with a live/dead cell assay (ab115347) (Abcam, Cambridge, UK) according to the manufacturer-suggested protocol. Immunofluorescence staining

(17) Establishment of long-term ostracod epidermal cultureIn Vitro Cellular & Developmental Biology - AnimalOctober 9, 2020Siân R. Morgan, Laura Paletto, Benjamin Rumney, Farhana T. Malik, Nick White, Philip N. Lewis, Andrew R. Parker, Simon Holden, Keith M. Meek, Julie Albonquantified as a percentage, was assessed at each time point, by using a live/dead assay kit (Abcam, ab115347), as described above for epidermal culture. Determination of Substrate for Epidermal Cell Culture Using

(18) Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe₃O₄ Nanocomposite in Photothermal Therapy Against CancerInternational Journal of NanomedicineAugust 25, 2020Claudia C Barrera, Helena Groot, Watson L Vargas, Diana M NarváezReverse Transcription kits were purchased from Thermo Fisher Scientific. Cell and dye cell assay (Kat. Ab115347) was supplied by Abcam. Specific primers for Hsp70 and Bcl-2 proteins were synthetized from specific sequences

(19) Flavagline synthetic derivative induces senescence in glioblastoma cancer cells without being toxic to healthy astrocytesScientific reportsAugust 13, 2020Harmouch E, Seitlinger J, Chaddad H, Ubeaud-Sequier G, Barths J, Saidu S, Désaubry L, Grandemange S, Massfelder T, Fuhrmann G, et al.pellet was resuspended in the live/dead assay dye solution for 10 min (Live/dead cell assay kit, Abcam, ab115347), and then visualized under an epifluorescence microscope (Leica DM4000 B). Immunofluorescence U87-MG

(20) FMRP(1-297)-tat restores ion channel and synaptic function in a model of Fragile X syndromeNature communicationsJune 2, 2020Zhan X, Asmara H, Cheng N, Sahu G, Sanchez E, Zhang FX, Zamponi GW, Rho JM, Turner RW.granule cell culture medium at 3 DIV or 7 DIV. At 8 DIV cells were labeled with a live-dead assay kit (ab115347, Abcam) before being analyzed with a flow cytometer BD LSR II (BD Biosciences) at 488 nm excitation

Be the First to Write a Review!

International Journal of Molecular Sciences

Live and Dead Cell Assay from Abcam

Abcam

Citations (34)

Reviews

Live and Dead Cell Assay from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map