Senescence Detection Kit ab65351 from Abcam

Product Specs
ItemSenescence Detection Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65351
Quantity250 test
Sample TypeTissue, Adherent cells
Assay Time1h 10m
ApplicationsCellular Assay
TypeEnzyme activity
Detection TargetSenescence-associated beta Galactosidase

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Time-resolved proteomic analyses of senescence highlight metabolic rewiring of mitochondriaLife science allianceSeptember 1, 2023Kim JY, Atanassov I, Dethloff F, Kroczek L, Langer T.were washed twice with PBS and subject to SA-β-Gal assay according to the manufacturer’s protocol (ab65351; Abcam). On the next day, the cells were washed twice with PBS and permeabilized with 0.2% TX-100/PBS

(2) Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm laboreLifeAugust 23, 2023Ciampa EJ, Flahardy P, Srinivasan H, Jacobs C, Tsai L, Karumanchi SA, Parikh SM.3. SA-βGal assay Adherent cells were fixed and stained for SA-βGal via a Senescence Detection kit (Abcam, ab65351) per manufacturer’s instructions. Representative images were used to calculate the fraction of SA-βGal-positive

(3) BRD4 Inhibition as a Strategy to Prolong the Response to Standard of Care in Estrogen Receptor-Positive Breast CancerCancersAugust 11, 2023Elshazly AM, Sinanian MM, Neely V, Chakraborty E, Alshehri MA, McGrath MK, Harada H, Schoenlein PV, Gewirtz DA.β-galactosidase activity to determine the senescence phenotype according to the manufacturer’s protocol (ab65351, Abcam). Phase contrast images of cells were taken using a brightfield inverted microscope (20X objective,

(4) A NRF2-induced secretory phenotype activates immune surveillance to remove irreparably damaged cellsRedox BiolAugust 10, 2023Liam Baird, Keiko Taguchi, Anqi Zhang, Yushi Takahashi, Takafumi Suzuki, Thomas W. Kensler, Masayuki Yamamotodays. Senescence associated-β-gal staining SA-β-gal staining was carried out using the Senescence Detection Kit (ab65351, Abcam) following the protocol provided by the manufacturer. The samples used in this assay were generated

(5) Premature aging and reduced cancer incidence associated with near-complete body-wide Myc inactivationCell ReportsAugust 1, 2023Huabo Wang, Jie Lu, Taylor Stevens, Alexander Roberts, Jordan Mandel, Raghunandan Avula, Bingwei Ma, Yijen Wu, Jinglin Wang, Clinton Van’t Van’t Land, et al.al.23 β-galactosidase staining Tissue sections were stained for β-galactosidase using a Senescence Detection Kit (ab65351) according to the directions of the supplier (Abcam, Inc., Waltham, MA). SDS-polyacrylamide gel electrophoresis

(6) Restoration of mitochondrial function by Spirulina polysaccharide via upregulated SOD2 in aging fibroblastsiScienceJuly 21, 2023Kayo Machihara, Shoma Oki, Yuka Maejima, Sou Kageyama, Ayumu Onda, Yurino Koseki, Yasuyuki Imai, Takushi Nambaweight.36 Detection of SA-β-gal activity Cellular SA-β-Gal activity was assessed using a senescence detection kit #ab65351 (Abcam, Cambridge, England) according to the manufacturer’s protocols. All images were obtained under

(7) Clearance of senescent cells reverts the cigarette smoke-induced lung senescence and airspace enlargement in p16-3MR miceAging cellJuly 1, 2023Kaur G, Muthumalage T, Rahman I.SA‐β‐Gal positive cells in control and treated samples, we employed Senescence detection kit (Cat# ab65351, Abcam). The SA‐β‐Gal staining solution was prepared as per manufacturer's protocol. Frozen tissue section

(8) Peelable Microneedle Patches Deliver Fibroblast Growth Factors to Repair Skin Photoaging DamageNanotheranosticsJune 19, 2023Guojun Yang, Shiqi Hu, Haiyue Jiang, Ke Chengβ-galactosidase (β-gal) staining Senescence was assessed in HDFs using the Senescence Detection kit (ab65351, Abcam, Cambridge, UK) according to the manufacturer's protocol. Briefly, the cells were washed with

(9) Crosstalk in skin: Loss of desmoglein 1 in keratinocytes inhibits BRAFV600E-induced cellular senescence in human melanocytesbioRxivMay 8, 2023Xin Tong, Hope E. Burks, Ziyou Ren, Jennifer L. Koetsier, Quinn R. Roth-Carter, Kathleen J. Greenchambers. Following lentiviral BRAF transduction and 3-day CM treatment, a SA-β-Gal staining kit (Abcam, ab65351) was used according to the manufacturer’s instructions. Senescent cell quantification was performed

(10) Senescence‐associated transcriptional derepression in subtelomeres is determined in a chromosome‐end‐specific mannerAging CellMay 1, 2023Martin Rey‐Millet, Mélanie Pousse, Chan Soithong, Jing Ye, Aaron Mendez‐Bermudez, Eric Gilsonβ‐galactosidase (SA‐β‐Gal) assay SA‐β‐Gal staining was performed using the Senescence Detection Kit (ab65351; Abcam) according to the manufacturer's instructions. Cells were visualized using phase‐contrast microscopy

(11) Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicityJCI insightApril 24, 2023Li S, Livingston MJ, Ma Z, Hu X, Wen L, Ding HF, Zhou D, Dong Z.and 20 μg/mL. SA-β-gal staining SA-β-gal staining was done with a Senescence Detection Kit (Abcam, ab65351) according to the manufacturer’s instructions. BUMPT cells were cultured on coverslips in 35 mm dishes.

(12) The Uremic Toxin Indoxyl Sulfate Accelerates Senescence in Kidney Proximal Tubule CellsToxins (Basel)March 25, 2023Yi Yang, Milos Mihajlovic, Manoe J. Janssen, Rosalinde Masereeuwmanufacturer’s instructions. A Senescence Detection Kit was used to identify the SA-gal-positive cells (ab65351, Abcam, Bristol, UK). The staining was evaluated for the appearance of a blue colour through an optical

(13) Reversine ameliorates hallmarks of cellular senescence in human skeletal myoblasts via reactivation of autophagyAging CellMarch 1, 2023Nika Rajabian, Debanik Choudhury, Izuagie Ikhapoh, Shilpashree Saha, Aishwarya S. Kalyankar, Pihu Mehrotra, Aref Shahini, Kendall Breed, Stelios T. AndreadisSenescence‐associated‐β‐galactosidase The SA‐β‐Gal activity was detected using the Senescence Detection Kit (ab65351, Abcam) according to the manufacturer's instructions. Cells were photographed using the Zeiss Axio Observer

(14) Effects of succinic acid on dermal fibroblasts during cultivation under extremely hypoxic conditionsBiochemistry and Biophysics ReportsMarch 1, 2023Tetiana Papurina, Olexiy Barsukov, Oksana Zabuga, Dmytro Krasnienkov, Eugen Denisdetection. For detection of SA-β-galactosidase activity in cells we used a Senescence Detection Kit (ab65351, Abcam, Cambridge, UK), which is designed for histochemical detection of SA-β-Gal activity in cultured cells

(15) Methionine adenosyltransferase2A inhibition restores metabolism to improve regenerative capacity and strength of aged skeletal muscleNature communicationsFebruary 16, 2023Rajabian N, Ikhapoh I, Shahini S, Choudhury D, Thiyagarajan R, Shahini A, Kulczyk J, Breed K, Saha S, Mohamed MA, et al.among others. Senescence-associated-β-galactosidase and periodic acid schiff (PAS) stain SA-β-Gal (ab65351, Abcam) and PAS stain (1016460001, Millipore sigma) were performed according to the manufacturer’s instructions.

(16) Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signalingNature communicationsJanuary 11, 2023Yun J, Hansen S, Morris O, Madden DT, Libeu CP, Kumar AJ, Wehrfritz C, Nile AH, Zhang Y, Zhou L, et al.organoid cultures. SA-β-Gal staining Staining was performed with the Senescence Detection Kit (Abcam, #ab65351) following the manufacturer’s protocol. Briefly, MEFs were fixed for 15 min with 1× fixation solution,

(17) Combination Therapies with CDK4/6 Inhibitors to Treat KRAS-Mutant Pancreatic CancerCancer ResearchJanuary 4, 2023Craig M. Goodwin, Andrew M. Waters, Jennifer E. Klomp, Sehrish Javaid, Kirsten L. Bryant, Clint A. Stalnecker, Kristina Drizyte-Miller, Bjoern Papke, Runying Yang, Amber M. Amparo, et al.indicated protein. Senescence was detected using the β-galactosidase based Senescence Detection Kit (ab65351, Abcam), using the recommended protocol. Cells were imaged and photographed on an Evos M7000. Separately,

(18) Higher Oxidative Stress in Endometriotic Lesions Upregulates Senescence-Associated p16(ink4a) and β-Galactosidase in Stromal CellsInternational journal of molecular sciencesJanuary 4, 2023Malvezzi H, Cestari BA, Meola J, Podgaec S.Assay Senescence-associated β-galactosidase (SA-β-gal) activity was detected using the senescence detection kit (Ab65351; Abcam, Cambridge, AX, UK) according to the manufacturer’s instructions. After H2O2 treatment, the stromal cells

(19) PCSK9 attenuates efferocytosis in endothelial cells and promotes vascular agingTheranosticsJanuary 1, 2023Liu S, Wu J, Stolarz A, Zhang H, Boerma M, Byrum SD, Rusch NJ, Ding Z.(Cell Signaling, 9860. In vivo, cell senescence in the aorta was examined with a Senescence Detection Kit (Abcam, ab65351) according to the provided protocol. Immunohistochemical and immunofluorescent staining. For immunohistochemical

(20) Inhibition of glutaminolysis restores mitochondrial function in senescent stem cellsCell reportsNovember 29, 2022Choudhury D, Rong N, Ikhapoh I, Rajabian N, Tseropoulos G, Wu Y, Mehrotra P, Thiyagarajan R, Shahini A, Seldeen KL, et al.Urea Assay Kit,Abcam,Cat# ab83362 DCF ROS/RNS Assay Kit,Abcam,Cat# ab238535 Senescence Detection Kit,Abcam,Cat# ab65351 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines Human:

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Senescence Detection Kit from Abcam

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Senescence Detection Kit from Abcam

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