PEG Virus Precipitation Kit ab102538 from Abcam

Product Specs
ItemPEG Virus Precipitation Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab102538
Quantity50 Test
Sample TypeOther biological fluids, Cell culture media
Detection Range40 - 100 %
SensitivityMore than 40 %

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) P selectin promotes SARS-CoV-2 interactions with platelets and the endotheliumJournal of Clinical InvestigationNovember 17, 2025Cesar L. Moreno, Fernanda V.S. Castanheira, Alberto Ospina Stella, Felicity Chung, Anupriya Aggarwal, Alexander J. Cole, Lipin Loo, Alexander Dupuy, Yvonne X. Kong, Lejla Hagimola, et al.1:10,000 Vybrant (V22887, Invitrogen) for 30 minutes at 37°C. This medium was resuspended with 1× PEG (ab102538, Abcam) and left overnight at 4°C. Medium was then centrifuged for 30 minutes at 3,200g at 4°C. The

(2) Disrupting the interaction between AMBRA1 and DLC1 prevents apoptosis while enhancing autophagy and mitophagyBiology OpenDecember 15, 2024Kate Hawkins, Meg Watt, Sébastien Gillotin, Maya Hanspal, Martin Helley, Jill Richardson, Nicola Corbett, Janet Brownleescentrifugation at 3200×g for 30 min at 4°C before being resuspended in 100 µl virus suspension solution (Abcam, ab102538), aliquoted and stored at −70°C. For transduction, 20 µl of this concentrated virus was added to 660

(3) Seaweed-derived fucoidans and rhamnan sulfates serve as potent anti-SARS-CoV-2 agents with potential for prophylaxisCarbohydrate PolymersAugust 1, 2024Yuefan Song, Amit Singh, Maisha M. Feroz, Shirley Xu, Fuming Zhang, Weihua Jin, Ambrish Kumar, Parastoo Azadi, Dennis W. Metzger, Robert J. Linhardt, et al.observed. Virus was purified from cell-free culture supernatants using an Abcam PEG purification kit (ab102538). The purified virus was resuspended in sterile phosphate buffered saline (PBS) and frozen at −80°C

(4) Synthetic gRNA/Cas9 ribonucleoprotein targeting HBV DNA inhibits viral replicationJournal of Medical VirologyMarch 22, 2024Jinyu Zhang, Yi Zhang, Sushant Khanal, Dechao Cao, Juan Zhao, Xindi Dang, Lam Ngoc Thao Nguyen, Madison Schank, Xiao Y. Wu, Yong Jiang, et al.gRNA RNP-treated HepDE19 cells were harvested and concentrated using a PEG concentration kit (Cat# ab102538, Abcam, Waltham, MA). For HBV infection, 2.5 × 105 HepG2-NTCP cells were seeded in a 12-well plate and

(5) Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus librariesNat CommunNovember 16, 2022Arpiar Saunders, Kee Wui Huang, Cassandra Vondrak, Christina Hughes, Karina Smolyar, Harsha Sen, Adrienne C. Philson, James Nemesh, Alec Wysoker, Seva Kashin, et al.aliquots or (2) from cell culture media used for rabies virus rescue after PEG-based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent, 5067–5579). To count barcode

(6) TSG101 associates with PARP1 and is essential for PARylation and DNA damageâ€induced NFâ€ÎºB activationThe EMBO JournalNovember 2, 2022Ahmet BuÄŸra Tufan, Katina Lazarow, Marina Kolesnichenko, Anje Sporbert, Jens Peter von Kries, Claus Scheidereitusing PEI. Lentiviruses were harvested and concentrated with PEG Virus Precipitation Kit (Abcam, Cat#ab102538) according to the manufacturer's protocol. The target cells were seeded into 24‐well plates at 20–40%

(7) Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic ProteinsBioengineering (Basel, Switzerland)February 25, 2022Akgül S, Offenhäuser C, Kordowski A, Day BW.VIC, Australia, cat. SLHVO33RS)PEG Virus Precipitation Kit (Abcam, Melbourne, VIC, Australia, cat. Ab102538)Hexadimethrine bromide (Polybrene) (Sigma Aldrich, North Ryde, NSW, Australia, cat. H9268)KnockOut™ DMEM/F-12

(8) Quantifying Absolute Neutralization Titers against SARS-CoV-2 by a Standardized Virus Neutralization Assay Allows for Cross-Cohort Comparisons of COVID-19 SeramBioFebruary 16, 2021Oguntuyo KY, Stevens CS, Hung CT, Ikegame S, Acklin JA, Kowdle SS, Carmichael JC, Chiu HP, Azarm KD, Haas GD, et al.filter (100-kDa cutoff, UFC910024; Millipore Sigma), or polyethylene glycol (PEG) precipitation (Abcam; ab102538). Concentrated viral particles were resuspended in 300 μl of PBS or Opti-MEM for further analysis. Western blots

(9) An EdU-based flow cytometry assay to evaluate chicken T lymphocyte proliferationBMC Veterinary ResearchDecember 1, 2020Karla Lucía F. Alvarez, Astrid Poma-Acevedo, Manolo Fernández-Sánchez, Manolo Fernández-Díaz(IBDV) was obtained from infected cell cultures and was concentrated using a PEG kit (Abcam, Catalog # ab102538) according to the manufacturer’s instructions. Subsequently, the virus was heat-inactivated in a water

(10) Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 seramedRxiv : the preprint server for health sciencesAugust 15, 2020Oguntuyo KY, Stevens CS, Hung CT, Ikegame S, Acklin JA, Kowdle SS, Carmichael JC, Chiu HP, Azarm KD, Haas GD, et al.Ultra centrifugal filter (100 kDa cutoff, Millipore Sigma, UFC910024), or PEG precipitation (Abcam, ab102538). Concentrated viral particles were resuspended in 300μL of PBS or Opti-MEM for further analysis. Western blots

(11) Caspase-1 interdomain linker cleavage is required for pyroptosisLife science allianceMarch 1, 2020Ball DP, Taabazuing CY, Griswold AR, Orth EL, Rao SD, Kotliar IB, Vostal LE, Johnson DC, Bachovchin DA.vector, 2 μg psPAX2, and 1 μg pMD2.G. The virus was concentrated using the PEG Virus Precipitation Kit (ab102538; Abcam), and CASP1−/− THP-1 cells made with sgRNA1 from our previous report (Okondo et al, 2017) were

(12) Spatial aspects of GDNF functions revealed in a compartmentalized microfluidic neuromuscular co-culture systemJournal of Cell ScienceMarch 15, 2015Eitan Erez Zahavi, Ariel Ionescu, Shani Gluska, Tal Gradus, Keren Ben-Yaakov, Eran Perlsonharvested at 2 days post-transfection, and concentrated ×100 using the PEG virus precipitation kit (Abcam, ab102538). Muscle infection and GDNF transport in axons Myoblasts and explants were plated in chambers and

(13) miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cellsJournal of neuroinflammationFebruary 18, 2015Sharma N, Verma R, Kumawat KL, Basu A, Singh SK.28°C. The supernatant was collected, and the virus was precipitated using PEG virus precipitation kit (#ab102538; Abcam, Cambridge, MA, USA). Virus titer was determined by using plaque assay. For plaque assay, 2 ×

(14) Rabies Virus Hijacks and accelerates the p75NTR retrograde axonal transport machineryPLoS pathogensAugust 1, 2014Gluska S, Zahavi EE, Chein M, Gradus T, Bauer A, Finke S, Perlson E.rRABV EGFP-P ΔG was concentrated from cell culture supernatants using the PEG virus precipitation kit (ab102538, Abcam). EGFP/sh-RNA-p75-EGFP lentivirus production HEK293t cells were transfected using calcium-phosphate

(15) Regulatory role of TRIM21 in the type-I interferon pathway in Japanese encephalitis virus-infected human microglial cellsJournal of neuroinflammationFebruary 1, 2014Manocha GD, Mishra R, Sharma N, Kumawat KL, Basu A, Singh SK.days. Post infection, supernatant was collected and precipitated using a PEG viral precipitation kit (#ab102538; Abcam, Cambridge, MA, USA). Plaque-forming units (PFU) of the propagated virus were determined by plaque

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PEG Virus Precipitation Kit from Abcam

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PEG Virus Precipitation Kit from Abcam

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