TMB ELISA Substrate (High Sensitivity) ab171523 from Abcam

Product Specs
ItemTMB ELISA Substrate (High Sensitivity)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab171523
Quantity100 mL, 1000 mL
ApplicationsELISA

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) ATAD2 mediates chromatin-bound histone chaperone turnovereLifeJanuary 20, 2026Ariadni Liakopoulou, Fayçal Boussouar, Daniel Perazza, Sophie Barral, Emeline Lambert, Tao Wang, Florent Chuffart, Ekaterina Bourova-Flin, Charlyne Gard, Denis Puthier, et al.Scientific,Cat# LCSF05, Commercial assay or kit,TMB ELISA Substrate (High Sensitivity),Abcam,Cat# AB171523, "Chemical compound, drug",Trans-Blot Turbo 5X Transfer Buffer,BioRad,Cat# 10026938, "Chemical compound,

(2) Hydrogel formulations for sustained-release of broadly neutralizing antibodiesJournal of Controlled ReleaseDecember 10, 2025Carolyn K. Jons, Catherine M. Kasse, Bryan T. Mayer, Ollivier Hyrien, Samya Sen, Emily L. Meany, Andrea I. d'Aquino, Priya Ganesh, Noah Eckman, Changxin Dong, et al.antibody incubation, the plates were washed ten times with PBS-T, and 50 μL of TMB solution (Abcam ab171523) was added to each well. The plate was incubated at room temperature for 3–10 minutes, or until color

(3) Role of Anti-Vinculin Quantitative ELISA Test in Diagnosing Irritable Bowel Syndrome and Inflammatory Bowel DiseaseMedical ArchivesJuly 12, 2025Tran Khanh Chi, Nguyen Thi Le My, Hoang Hai Yen, Dinh Thi Q Ngoc, Nguyen Thu Huong, Dao Viet Hang, Nguyen Minh Hienbuffer; anti-vinculin antibody (Ab219649, Abcam); HRP-conjugated anti-human IgG (Ab102420, Abcam); TMB (Ab171523, Abcam); H₂SO₄ (Ab210900, Abcam); Tween 20; RNase/DNase-free water; 96-well plates; pipettes; 15/50

(4) Histotripsy-focused ultrasound treatment abrogates tumor hypoxia responses and stimulates anti-tumor immune responses in melanomaMolecular Cancer TherapeuticsJuly 2, 2025Brian Song, Heineken Queen, Sarah F. Ferris, Reliza McGinnis, Chaitanya Karanam, Natalie Gatteno, Katherine Buglak, Hanna Kim, Jintao Xu, Kristie D. Goughenour, et al.hours. The plate was washed and 100 μL of Substrate Solution (Abcam, Cambridge, United Kingdom; Cat # ab171523) was added and incubated in the dark at RT for 20 minutes. 50 μL of Stop Solution (Abcam; Cat # ab210900)

(5) Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cellsAntibody TherapeuticsJune 27, 2025Scott McComb, Bianca Dupont, Alex Shepherd, Bigitha Bennychen, Anne Marcil, Laura Tamblyn, Shalini Raphael, Joey Sheff, Greg Hussack, Anna N Moraitis, et al.ImmunoResearch 016-030-084) for 1 hour, then washed, and developed with TMB ELISA substrate (Abcam ab171523) for 5 minutes before stopping the reaction with 1 M phosphoric acid. Serum SDS-PAGE Fractionated

(6) Gut microbiota‐derived trimethylamine‐N‐oxide inhibits SIRT1 to regulate SM22α‐mediated smooth muscle cell inflammation and promote atherosclerosis progressionJ Cell Commun SignalJune 6, 2025Yajuan Yin, Mei Wei, Xiufang Jiang, Mei Liu, Xiaocui Shi, Xiao Zhang, Le Wang, Gang Liu, Mingqi Zheng, Fangfang Maand TNF‐α (ab208348, Abcam) were measured using the enzyme‐linked immunosorbent assay (ELISA) kit (ab171523, Abcam). To prepare cell lysates, the cells were washed with PBS and collected, followed by centrifugation

(7) Generation of an inflammatory niche in a hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccinesScience advancesApril 11, 2025Meany EL, Klich JH, Jons CK, Mao T, Chaudhary N, Utz A, Baillet J, Song YE, Saouaf OM, Ou BS, et al.plates were developed for 6 min with high-sensitivity tetramethylbenzidine (TMB) substrate (Abcam, ab171523), and the reaction was stopped with 1 N HCl. Absorbance was read at 450 nm with a Synergy H1 microplate

(8) Selective promotion of sensory innervation-mediated immunoregulation for tissue repairScience advancesMarch 21, 2025Xu K, Wu K, Chen L, Zhao Y, Li H, Lin N, Ye Z, Xu J, Huang D, Huang X.antibody (ab6789, Abcam) at 4°C for an hour each. The color reaction was done by the TMB ELISA substrate (ab171523, Abcam), and the absorbance of each well was detected at 450 nm by a spectrophotometer (ShimadzuUV-3150). For

(9) The impact of the mRNA COVID-19 vaccine on the Th-like cytokine profile in individuals with no history of COVID-19: insights into autoimmunity targeting heat shock proteinsFront ImmunolMarch 14, 2025Stefan Tukaj, Magdalena Sitna, Krzysztof Sitko0.1% BSA, for 60 minutes at RT. The enzymatic reaction was visualized using TMB substrate solution (ab171523, Abcam), and the reaction was halted by the addition of H2SO4. Optical density (OD) measurements were

(10) Recombinant expression of receptor binding domains of all eight subtypes of botulinum neurotoxin type A for generation of antitoxins with broad reactivityF1000ResearchFebruary 5, 2025Nga Quynh Pham, Tam Trang Mai, Tran Bao Anh Dang, Ly Huong Tran, Quynh Mai Vu, Chien Trong Nguyen, Anh Thi Phuong Tran, Tran Nhat Minh Dang, Van Anh Tran, Thinh Huy Tran, et al.(Sigma-Aldrich, cat. number P3563) and reactions were developed with TMB substrate (Abcam, cat. number AB171523) and read at 450 nm. EC 50 values were calculated via non-linear regression analysis using GraphPad

(11) Enhancement of mycobacterial pathogenesis by host interferon-γCellular and Molecular Life SciencesDecember 1, 2024Huynh Tan Hop, Pao-Chi Liao, Hsin-Yi Wumouse IL1β protein (#ab259421), recombinant mouse TNFα protein (#ab259411), and TMB ELISA substrate (#ab171523) from Abcam; mouse monoclonal anti-β-actin antibody (#3700S) from Cell Signaling; recombinant mouse

(12) Neuroendocrine effects of the duper mutation in Syrian hamsters: a role for Cryptochrome 1Frontiers in PhysiologyFebruary 20, 2024Emily N. C. Manoogian, Michael Seifu Bahiru, Emily Jue Wang, Mary Holder, Eric L. Bittmanperoxidase-conjugated goat anti-rabbit (D048701-2; DakoCytomation) was detected with ABCAM high sensitivity TMB (Cat #ab171523). The reaction was stopped by the addition of Abcam Cat#ab171529. Absorbance was read at a wavelength

(13) Surface Oxygen Deficiency Enabled Spontaneous Antiprotein Fouling in WO3 Nanosheets for Biosensing in Biological FluidsAnalytical ChemistryJanuary 16, 2024Guozhen He, Tao Dong, Zhaochu Yang, Bjo̷rn Torger Stokke, Zhuangde Jiangincubating at 37 °C for 45 min, we added 100 μL of 3′,3′,5′,5′ tetramethylbenzidine (TMB) substrate (Cat No. ab171523; Abcam) per well for color rendering. Until we observed discoloration at room temperature, we added

(14) Identification and characterization of two salmon louse heme peroxidases and their potential as vaccine antigensiScienceOctober 20, 2023Elisabeth Gislefoss, Amr Ahmed Abdelrahim Gamil, Aina-Cathrine Øvergård, Øystein EvensenGelCode™ Blue Safe Protein Stain,ThermoFisher,Cat# 24594 Montanide ISA 763 A VG,Seppic,N/A TMB ELISA Substrate (High Sensitivity),Abcam,Cat# ab171523 LsPxtl-2,This paper,N/A ,, Critical commercial assays,Critical commercial assays,Critical commercial

(15) Structural basis for antibody-mediated neutralization of lymphocytic choriomeningitis virusCell chemical biologyApril 20, 2023Moon-Walker A, Zhang Z, Zyla DS, Buck TK, Li H, Diaz Avalos R, Schendel SL, Hastie KM, Crotty S, Saphire EO.ExpiFectamine CHO Transfection Kit,Thermo Fisher Scientific,Cat# A29129 TMB ELISA Substare (High Sensitivity),Abcam,Cat# ab171523 650nm Stop Solution for TMB Substrate,Abcam,Cat# ab171531 Experimental models: Cell lines,Experimental models

(16) Subcutaneous delivery of an antibody against SARS-CoV-2 from a supramolecular hydrogel depot†Biomaterials ScienceMarch 14, 2023Catherine M. Kasse, Anthony C. Yu, Abigail E. Powell, Gillie A. Roth, Celine S. Liong, Carolyn K. Jons, Awua Buahin, Caitlin L. Maikawa, Xueting Zhou, Sawsan Youssef, et al.Plates were washed 5x in PBS-T before developing with TMB ELISA Substrate (High Sensitivity) (Abcam, ab171523). Plates were allowed to develop for 3 min before the reaction was stopped by adding 1 N HCl. Absorbance

(17) Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli DetectionACS applied materials & interfacesFebruary 22, 2023Zhao L, Rosati G, Piper A, de Carvalho Castro E Silva C, Hu L, Yang Q, Della Pelle F, Alvarez-Diduk RR, Merkoçi A.(Madrid, Spain). HRP labeled antibodies (ab20425) and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (ab171523) were purchased from Abcam plc (Cambridge, UK). Escherichia coli (E. coli, CECT 4972) and Staphylococcus aureus subsp. Aureus

(18) SARS-CoV-2 humoral and cellular immunity following different combinations of vaccination and breakthrough infectionNature communicationsFebruary 2, 2023Pušnik J, Monzon-Posadas WO, Zorn J, Peters K, Baum M, Proksch H, Schlüter CB, Alter G, Menting T, Streeck H.were then washed and 100 µl of the substrate was added (TMB ELISA Substrate, High Sensitivity, Abcam, ab171523). The reaction took place at room temperature for 5 min, followed by the addition of 100 µl of 1 M H2SO4.

(19) Stable High‐Concentration Monoclonal Antibody Formulations Enabled by an Amphiphilic Copolymer ExcipientAdvanced TherapeuticsJanuary 23, 2023John H. Klich, Catherine M. Kasse, Joseph L. Mann, Yaoqi Huang, Andrea I. d'Aquino, Abigail K. Grosskopf, Julie Baillet, Gerald G. Fuller, Eric A. Appelwashed 10 times and then developed by adding 50 μL of TMB ELISA Substrate (High Sensitivity) (Abcam, ab171523). Development was stopped after 2.5 min by adding 1 N hydrochloric acid. PGT121 concentration was measured

(20) Evaluation of Potential Peptide-Based Inhibitors against SARS-CoV-2 and Variants of ConcernBioMed research internationalJanuary 1, 2023Boshah H, Samkari F, Valle-Pérez AU, Alsawaf SM, Aldoukhi AH, Bilalis P, Alshehri SA, Susapto HH, Hauser CAE.from Fisher Scientific (096292) and S1 protein from Sino Biological (40591-V08H). Both TMB substrate, ab171523, and spike antibody, ab273073, were provided by Abcam. ELISA stop solution was acquired from Invitrogen

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TMB ELISA Substrate (High Sensitivity) from Abcam

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TMB ELISA Substrate (High Sensitivity) from Abcam

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