TMB ELISA Substrate (High Sensitivity) ab171523 from Abcam

Product Specs
ItemTMB ELISA Substrate (High Sensitivity)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab171523
Quantity100 mL, 1000 mL
ApplicationsELISA

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) EV-LYSG101: Extracellular Vesicles Loaded With Lysin LYSG101 for Potent Anti-Staphylococcal ActivityJ Extracell BiolAugust 6, 2026Dominique B. Hoelzinger, Ava M. Koehler de Celaya, Cheryl E. Myers, Natasha P. Dyal, Christopher F. Saling, Raymond Schuch, Gina A. Suhmin at room temperature with four washes of TBS‐T. (7) 100 µL TMB ELISA substrate high sensitivity (AB171523, Abcam) was added for 2–3 min. (8) The enzymatic reaction was stopped with 1% HCl. (9) Plates were read

(2) mRNA vaccination using peptide nanoparticles triggers a strong immune response against endogenous GPC2 in a murine neuroblastoma modelMol Ther OncolJune 18, 2026Ellen King, Chayanika Saha, Rabia Saleem, Binyumeng Jiang, Eve O'Donoghue, Federica Cottone, Helen O. McCarthy, Olga PiskarevaP1379), Triton X-100 (Sigma-Aldrich, UK, cat. no. X100-5 mL), TMB ELISA Substrate (Abcam UK, cat. no. ab171523), Stop solution1x (Abcam, UK, cat. no. ab210900), RNAase (Thermo Fisher Scientific, UK, cat. no. EN0531),

(3) Machine Learning‐Enhanced Ultrasensitive Immuno‐CRISPR Array Facilitates Early Diagnosis of Alzheimer's Disease by Detecting Multiple Plasma BiomarkersAdvanced ScienceJune 9, 2026Liding Zhang, Changwen Yang, Qian Yao, Xuewei Du, Shuai Ding, Yaoqiang Shi, Can Sheng, Ming Wang, Ying Han, Haiming Luo(1:8000) was added and incubated at 37°C for 1 h. Finally, a soluble TMB substrate solution (Abcam, cat: ab171523) was added to detect the immune response, and the absorbance of each well was detected at 450 nm. For

(4) Microglia-independent rAAV-induced inflammation causes persistent ocular immune dysregulation rescued by S1P receptor modulationMolecular TherapyMay 6, 2026Philip M. Langer, Alison J. Clare, Xudong Peng, Katherine L. Costello, Suci Cendanawati, Leslie L. Wilson, Amy Ward, Oliver H. Bell, Colin J. Chu, Ying Kai Chan, et al.for 1 h at 20°C. Subsequently, the plate was incubated with TMB ELISA Substrate (High Sensitivity) (ab171523, Abcam) for 10 min, and the reaction was halted with ELISA Stop Solution (catalog no. SS03, Thermo Fisher Scientific

(5) Severe hypoxia drives loss of ST6GAL1-mediated α2,6-sialylation in the epicardial secretome impairing angiogenic activityBiology OpenApril 15, 2026Junqing Zhang, Harry J. Whitwell, Costanza Emanueli, Pilar Ruiz-Lozanowashed five times with TBS-T and following once with PBS, and subsequently 100 µl of TMB substrate (ab171523, pre-equilibrated to room temperature) was added to each well. The reaction was allowed to proceed for

(6) Radiofrequency radiation-induced changes in Leydig cell functionScientific ReportsMarch 25, 2026Pooja Jangid, Umesh Rai, Jayesh Kumar Sevak, Ravi Ranjan, Sanjay Singh, Rajeev SinghML023, HiMedia Laboratories Pvt. Ltd., Thane, India), tetramethylbenzidine (TMB) substrate (Abcam, AB171523, Abcam plc, Cambridge, United Kingdom), Heat-inactivated fetal bovine serum (FBS) (Himedia, RM10951,

(7) ATAD2 mediates chromatin-bound histone chaperone turnoverElifeJanuary 20, 2026Ariadni Liakopoulou, Fayçal Boussouar, Daniel Perazza, Sophie Barral, Emeline Lambert, Tao Wang, Florent Chuffart, Ekaterina Bourova-Flin, Charlyne Gard, Denis Puthier, et al.Scientific,Cat# LCSF05, Commercial assay or kit,TMB ELISA Substrate (High Sensitivity),Abcam,Cat# AB171523, "Chemical compound, drug",Trans-Blot Turbo 5X Transfer Buffer,BioRad,Cat# 10026938, "Chemical compound,

(8) ATAD2 mediates chromatin-bound histone chaperone turnovereLifeJanuary 20, 2026Ariadni Liakopoulou, Fayçal Boussouar, Daniel Perazza, Sophie Barral, Emeline Lambert, Tao Wang, Florent Chuffart, Ekaterina Bourova-Flin, Charlyne Gard, Denis Puthier, et al.Scientific,Cat# LCSF05, Commercial assay or kit,TMB ELISA Substrate (High Sensitivity),Abcam,Cat# AB171523, "Chemical compound, drug",Trans-Blot Turbo 5X Transfer Buffer,BioRad,Cat# 10026938, "Chemical compound,

(9) Hydrogel formulations for sustained-release of broadly neutralizing antibodiesJournal of Controlled ReleaseDecember 10, 2025Carolyn K. Jons, Catherine M. Kasse, Bryan T. Mayer, Ollivier Hyrien, Samya Sen, Emily L. Meany, Andrea I. d'Aquino, Priya Ganesh, Noah Eckman, Changxin Dong, et al.antibody incubation, the plates were washed ten times with PBS-T, and 50 μL of TMB solution (Abcam ab171523) was added to each well. The plate was incubated at room temperature for 3–10 minutes, or until color

(10) Role of Anti-Vinculin Quantitative ELISA Test in Diagnosing Irritable Bowel Syndrome and Inflammatory Bowel DiseaseMedical ArchivesJuly 12, 2025Tran Khanh Chi, Nguyen Thi Le My, Hoang Hai Yen, Dinh Thi Q Ngoc, Nguyen Thu Huong, Dao Viet Hang, Nguyen Minh Hienbuffer; anti-vinculin antibody (Ab219649, Abcam); HRP-conjugated anti-human IgG (Ab102420, Abcam); TMB (Ab171523, Abcam); H₂SO₄ (Ab210900, Abcam); Tween 20; RNase/DNase-free water; 96-well plates; pipettes; 15/50

(11) Histotripsy-focused ultrasound treatment abrogates tumor hypoxia responses and stimulates anti-tumor immune responses in melanomaMolecular Cancer TherapeuticsJuly 2, 2025Brian Song, Heineken Queen, Sarah F. Ferris, Reliza McGinnis, Chaitanya Karanam, Natalie Gatteno, Katherine Buglak, Hanna Kim, Jintao Xu, Kristie D. Goughenour, et al.hours. The plate was washed and 100 μL of Substrate Solution (Abcam, Cambridge, United Kingdom; Cat # ab171523) was added and incubated in the dark at RT for 20 minutes. 50 μL of Stop Solution (Abcam; Cat # ab210900)

(12) Histotripsy-focused ultrasound treatment abrogates tumor hypoxia responses and stimulates anti-tumor immune responses in melanomaMol Cancer TherJuly 2, 2025Brian Song, Heineken Queen, Sarah F. Ferris, Reliza McGinnis, Chaitanya Karanam, Natalie Gatteno, Katherine Buglak, Hanna Kim, Jintao Xu, Kristie D. Goughenour, et al.hours. The plate was washed and 100 μL of Substrate Solution (Abcam, Cambridge, United Kingdom; Cat # ab171523) was added and incubated in the dark at RT for 20 minutes. 50 μL of Stop Solution (Abcam; Cat # ab210900)

(13) Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cellsAntibody TherapeuticsJune 27, 2025Scott McComb, Bianca Dupont, Alex Shepherd, Bigitha Bennychen, Anne Marcil, Laura Tamblyn, Shalini Raphael, Joey Sheff, Greg Hussack, Anna N Moraitis, et al.ImmunoResearch 016-030-084) for 1 hour, then washed, and developed with TMB ELISA substrate (Abcam ab171523) for 5 minutes before stopping the reaction with 1 M phosphoric acid. Serum SDS-PAGE Fractionated

(14) Gut microbiota‐derived trimethylamine‐N‐oxide inhibits SIRT1 to regulate SM22α‐mediated smooth muscle cell inflammation and promote atherosclerosis progressionJ Cell Commun SignalJune 6, 2025Yajuan Yin, Mei Wei, Xiufang Jiang, Mei Liu, Xiaocui Shi, Xiao Zhang, Le Wang, Gang Liu, Mingqi Zheng, Fangfang Maand TNF‐α (ab208348, Abcam) were measured using the enzyme‐linked immunosorbent assay (ELISA) kit (ab171523, Abcam). To prepare cell lysates, the cells were washed with PBS and collected, followed by centrifugation

(15) Generation of an inflammatory niche in a hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccinesScience advancesApril 11, 2025Meany EL, Klich JH, Jons CK, Mao T, Chaudhary N, Utz A, Baillet J, Song YE, Saouaf OM, Ou BS, et al.plates were developed for 6 min with high-sensitivity tetramethylbenzidine (TMB) substrate (Abcam, ab171523), and the reaction was stopped with 1 N HCl. Absorbance was read at 450 nm with a Synergy H1 microplate

(16) Selective promotion of sensory innervation-mediated immunoregulation for tissue repairScience advancesMarch 21, 2025Xu K, Wu K, Chen L, Zhao Y, Li H, Lin N, Ye Z, Xu J, Huang D, Huang X.antibody (ab6789, Abcam) at 4°C for an hour each. The color reaction was done by the TMB ELISA substrate (ab171523, Abcam), and the absorbance of each well was detected at 450 nm by a spectrophotometer (ShimadzuUV-3150). For

(17) The impact of the mRNA COVID-19 vaccine on the Th-like cytokine profile in individuals with no history of COVID-19: insights into autoimmunity targeting heat shock proteinsFront ImmunolMarch 14, 2025Stefan Tukaj, Magdalena Sitna, Krzysztof Sitko0.1% BSA, for 60 minutes at RT. The enzymatic reaction was visualized using TMB substrate solution (ab171523, Abcam), and the reaction was halted by the addition of H2SO4. Optical density (OD) measurements were

(18) Recombinant expression of receptor binding domains of all eight subtypes of botulinum neurotoxin type A for generation of antitoxins with broad reactivityF1000ResearchFebruary 5, 2025Nga Quynh Pham, Tam Trang Mai, Tran Bao Anh Dang, Ly Huong Tran, Quynh Mai Vu, Chien Trong Nguyen, Anh Thi Phuong Tran, Tran Nhat Minh Dang, Van Anh Tran, Thinh Huy Tran, et al.(Sigma-Aldrich, cat. number P3563) and reactions were developed with TMB substrate (Abcam, cat. number AB171523) and read at 450 nm. EC 50 values were calculated via non-linear regression analysis using GraphPad

(19) Enhancement of mycobacterial pathogenesis by host interferon-γCellular and Molecular Life SciencesDecember 1, 2024Huynh Tan Hop, Pao-Chi Liao, Hsin-Yi Wumouse IL1β protein (#ab259421), recombinant mouse TNFα protein (#ab259411), and TMB ELISA substrate (#ab171523) from Abcam; mouse monoclonal anti-β-actin antibody (#3700S) from Cell Signaling; recombinant mouse

(20) Neuroendocrine effects of the duper mutation in Syrian hamsters: a role for Cryptochrome 1Frontiers in PhysiologyFebruary 20, 2024Emily N. C. Manoogian, Michael Seifu Bahiru, Emily Jue Wang, Mary Holder, Eric L. Bittmanperoxidase-conjugated goat anti-rabbit (D048701-2; DakoCytomation) was detected with ABCAM high sensitivity TMB (Cat #ab171523). The reaction was stopped by the addition of Abcam Cat#ab171529. Absorbance was read at a wavelength

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TMB ELISA Substrate (High Sensitivity) from Abcam

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TMB ELISA Substrate (High Sensitivity) from Abcam

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