CFSE - Cell Labeling Kit ab113853 from Abcam

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ItemCFSE - Cell Labeling Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab113853
Quantity1000 Test

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) High-dose irradiation promotes macrophage-mediated engulfment of triple-negative breast cancer through M1 polarization via the IKZF1-CCL5 axisCell Communication and SignalingFebruary 10, 2026Han-Hsi Kuo, Hui-Wen Chan, Chun-Yu Liu, Ming-Huang Chen, Yi-Jang Lee, Hui-Yen Chuang, Yu-Chan Changbeads). Direct co-culture 4T1 cells were pre-labeled with carboxyfluorescein succinimidyl ester (CFSE) (ab113853, Abcam), and co-cultured with RAW 264.7 cells that had been irradiated with 0.5 or 8 Gy, 24 h post-irradiation.

(2) Hypoxic migrasomes drive colorectal cancer liver metastasis by mediating CD5L+ macrophage efferocytosis via NRP2/PROX1 axisJ Transl MedDecember 8, 2025Yuqi Luo, Yiwen Huang, Yaoxing Huang, Longzhen Chenknockdown were resuspended at a final concentration of 1 × 10⁵ cells/mL. MC38 cells were labeled with CFSE (ab113853, Abcam), after which the culture medium was removed and the cells were resuspended in serum-free medium

(3) Co-encapsulation of hepatocytes, mesenchymal stem cells and growth factor in arginine-glycine-aspartate functionalized microbeads for liver diseaseRegenerative BiomaterialsSeptember 16, 2025Su Yee Win, Pinunta Nittayacharn, Arkhom Saingam, Khanit Sa-ngiamsuntorn, Norased Nasongklabovine serum (FBS) was purchased from Gibco, UK. Carboxyfluorescein diacetate succinimidyl ester (CFSE, ab113853) was sourced from Abcam, UK. Hoechst 33342 and PI/RNase staining solution (FxCycle) were purchased from

(4) Dendritic cell membrane-based DCsLipo@MnO2@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancerMater Today BioJuly 1, 2025Wenjin Zhong, Pengcheng Wang, Jintian Wang, Yuejia Zhu, Liquan Chen, Yilong Qian, Xiang Huang, Kai Yenanomaterials on T cell proliferation were detected by a CFSE fluorescent cell labeling kit (Abcam, ab113853). The CFSE dye was diluted to 1 μM with PBS and added to the activated T cells. Under room temperature,

(5) Stabilized iRGD modification enhances NY-ESO-1 TCR-T infiltration in solid tumors and synergizes with PD-1 blockadeCancer Immunol ImmunotherMay 30, 2025Yirong Wu, Lanqun Qin, Jiayu Wang, Ziyao Xie, Xinyu Su, Xiang Li, Yueling Yang, Rong Huang, Mengke Zhao, Lianjun Zhao, et al.cell proliferation assessment, 1 μL of 5 mM carboxyfluorescein succinimidyl ester (CFSE) (Abcam, Cat# ab113853, UK) was added to 105 cells and incubated at 37 °C for 10 min. The reaction was terminated immediately

(6) Pyrimidine synthesis enzyme CTP synthetase 1 suppresses antiviral interferon induction by deamidating IRF3ImmunityJanuary 14, 2025Rao Y, Qin C, Savas AC, Liu Q, Feng S, Hou G, Xie T, Feng P.554412 FITC anti-human/mouse Granzyme B Antibody,BioLegend,Cat#: 515403 CFSE - Cell Labeling Kit,Abcam,Cat#: ab113853 Chemicals,Chemicals,Chemicals [α-P32]-ATP,PerkinElmer,Cat#: BLU003H250UC [amide-15N] Glutamine,Cambridge

(7) CCR2 cooperativity promotes hematopoietic stem cell homing to the bone marrowScience advancesSeptember 20, 2024Hurwitz SN, Kobulsky DR, Jung SK, Chia JJ, Butler JM, Kurre P.allowed to adhere for 2 days. For EV uptake visualization, HSPC EVs were dyed with 40 μM CFSE (Abcam, #ab113853) for 10 min, then washed with PBS, and repelleted by ultracentrifugation at 100,000g for 2 hours. EVs

(8) Molecular and Functional Cargo of Plasma-Derived Exosomes in Patients with Hereditary Hemorrhagic TelangiectasiaJournal of clinical medicineSeptember 13, 2024Wang Y, Hofmann L, Huber D, Lochbaum R, Ludwig S, Brunner C, Hoffmann TK, Lehner R, Theodoraki MN.calculated using ImageJ 1.53k macro [36]. 2.9. Proliferation Assay HUVECs were labeled using CFSE (Abcam, ab113853) according to the manufacturer’s protocol and incubated with PBS, HD or HHT exosomes for 24 h. Fluorescence

(9) Apoptosis recognition receptors regulate skin tissue repair in miceeLifeDecember 21, 2023Justynski O, Bridges K, Krause W, Forni MF, Phan QM, Sandoval-Schaefer T, Carter K, King DE, Hsia HC, Gazes MI, et al.Ly6G beads (Miltenyi Biotech 130-120-337). Neutrophils were stained with CFSE cell labeling kit (Abcam ab113853) for 15 min. Stained neutrophils were incubated overnight in RPMI medium to induce apoptosis. 10,000

(10) T cell-specific P2RX7 favors lung parenchymal CD4(+) T cell accumulation in response to severe lung infectionsCell reportsNovember 28, 2023Santiago-Carvalho I, Almeida-Santos G, Macedo BG, Barbosa-Bomfim CC, Almeida FM, Pinheiro Cione MV, Vardam-Kaur T, Masuda M, Van Dijk S, Melo BM, et al.M0678-1VL RPMI 1640 Medium,Gibico,Cat# 11875093 Ketamine,Syntec,N/A Xylazine,Syntec,N/A CFSE,Abcam,Cat# ab113853 CellTrace Violet,Thermo Fisher Scientific,Cat# C34557 Fingolimod (FTY720),Selleck Chemicals,Cat# S5002 Collagenase

(11) Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cellsStem cell research & therapyAugust 21, 2023Rosado-Galindo H, Domenech M.cultured for additional 24 h. PBMCs were labeled using 10 µM carboxyfluorescein succinimidyl ester—CFSE (ab113853, Abcam)—and seeded at a 1:0.5 ratio (PBMCs: hBM-MSCs) and co-cultured with hBM-MSCs for four days using

(12) Pinostrobin and Tectochrysin Conquer Multidrug-Resistant Cancer Cells via Inhibiting P-Glycoprotein ATPasePharmaceuticals (Basel)January 29, 2023I-Ting Wu, Chan-Yen Kuo, Ching-Hui Su, Yu-Hsuan Lan, Chin-Chuan Hungpinostrobin and tectochrysin on cell proliferation were evaluated using the CFSE Cell Labeling Kit (ab113853; Abcam, Cambridge, UK). The cells were harvested and suspended in 2 mL PBS. CFSE stock solution (10

(13) BETi enhance ATGL expression and its lipase activity to exert their antitumoral effects in triple-negative breast cancer (TNBC) cellsJournal of experimental & clinical cancer research : CRJanuary 6, 2023Rossi T, Zamponi R, Chirico M, Pisanu ME, Iorio E, Torricelli F, Gugnoni M, Ciarrocchi A, Pistoni M.(5(6)-Carboxyfluorescein N-hydroxysuccinimidylester) staining by CFSE - Cell Labeling Kit (Abcam, #ab113853) following manufacture’s protocol. Briefly 5 × 104 cells/condition were washed with PBS and resuspended

(14) GMP‐compliant manufacturing of biologically active cell‐derived vesicles produced by extrusion technologyJournal of Extracellular BiologyDecember 25, 2022Hui‐Chong Lau, Dong Woo Han, Jinhee Park, Edwine Lehner, Carina Kals, Claudia Arzt, Elisabeth Bayer, Daniela Auer, Tanja Schally, Eva Grasmann, et al.24 h. Cells were labelled with 5(6)‐carboxyfluorescein diacetate N‐succinimidyl ester (CFSE; Abcam, #ab113853) at a final concentration of 5 μM for 30 min in serum‐free media to normalise the respective cell mass.

(15) Comparing the immunomodulatory properties of equine BM-MSCs culture expanded in autologous platelet lysate, pooled platelet lysate, equine serum and fetal bovine serum supplemented culture mediaFrontiers in Veterinary ScienceAugust 25, 2022Kayla M. Even, Angela M. Gaesser, Sarah A. Ciamillo, Renata L. Linardi, Kyla F. Ortvedlabeled with carboxy-fluorescein diacetate succinimidyl ester (CFSE) fluorescent labeling solution (ab113853, Abcam, Cambridge, MA) as per the manufacturer's protocol. Briefly, PBMCs (50 x 106 per mL of PBS) were

(16) Imaging-Guided Bioreactor for Generating Bioengineered Airway TissueJournal of Visualized ExperimentsApril 25, 2022Seyed Mohammad Mir, Jiawen Chen, Meghan R. Pinezich, John D. O’Neill, Brandon A. Guenthart, Gordana Vunjak-Novakovic, Jinho Kimcube,Thorlabs,C4W, Carbon double-sided conductive tape,TED PELLA,16073, CFSE labelling kit,Abcam,ab113853, Citrisolv (clearing agent),Decon,1061, C-mount adapter,Thorlabs,SM1A9, Collagen I,Advanced BioMatrix,5153, Conductive

(17) Imaging-guided bioreactor for de-epithelialization and long-term cultivation of ex vivo rat tracheaLab on a ChipMarch 1, 2022Seyed Mohammad Mir, Jiawen Chen, Meghan R. Pinezich, John D. O'Neill, Sarah X. L. Huang, Gordana Vunjak-Novakovic, Jinho Kimtrachea was labeled fluorescently using carboxyfluorescein succinimidyl ester (CFSE) cell labeling kit (ab113853, Abcam). The tissue labeling was performed according to the company’s instruction prior to the de-epithelialization

(18) Extracellular Vesicles Derived-LAT1 mRNA as a Powerful Inducer of Colorectal Cancer Aggressive PhenotypeBiologyJanuary 15, 2022Almeida C, Teixeira AL, Dias F, Machado V, Morais M, Martins G, Palmeira C, Sousa ME, Godinho I, Batista S, et al.EVs’ quantification in our isolates was made using a CFSE (Carboxyfluorescein succinimidyl ester) (ab113853—Abcam®, Cambridge, UK) staining protocol, optimized for a conventional flow cytometer, using a BD FACS

(19) Microfluidic Printing of Tunable Hollow Microfibers for Vascular Tissue EngineeringAdvanced Materials TechnologiesAugust 30, 2021Zhuhao Wu, Hongwei Cai, Zheng Ao, Junhua Xu, Samuel Heaps, Feng Guo(EGM-2) were purchased from Lonza (Walkersville, MD, USA). Carboxyfluorescein succinimidyl ester (CFSE, ab113853) and ethidium homodimer-1 (ethd-1, E1169) and cell tracker were obtained from Abcam (Cambridge, MA,

(20) Tumor-derived exosomal microRNA-7-5p enhanced by verbascoside inhibits biological behaviors of glioblastoma in vitro and in vivoMolecular Therapy - OncolyticsMarch 26, 2021Huan Wang, Jiugeng Feng, Fan Ao, Yiqiang Tang, Pengliang Xu, Min Wang, Min Huangphosphotungstic acid for 1 min and then rinsed with PBS. Uptake of CFSE-traced exosomes by GBM cells CFSE dye (ab113853; Abcam, Cambridge, UK) was diluted at a ratio of 1:1,000, supplemented with 20 μg of the exosome suspension

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CFSE - Cell Labeling Kit from Abcam

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