NADH and NADPH Assay Kit (Colorimetric) ab186031 from Abcam

Product Specs
ItemNADH and NADPH Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab186031
Quantity400 Test
Sample TypeCell culture supernatant
SensitivityMore than 3 µM
Assay Time2h 0m
Detection Range3.13 - 100 µM

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+44 (0) 1223 771600

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(1) Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell statesStem Cell Research & TherapyDecember 1, 2025Axelle Bouche, Diego Michel, Perrine Castets, Didier Hannouche, Thomas LaumonierMuRCs and MBs using the NAD/NADH Quantification Kit (MAK037, Sigma Aldrich) and the NADPH Assay Kit (ab186031, Abcam). For NADPH analysis, cells were lysed in the provided buffer (1 × 106 cells/100 µl) prio to

(2) Plant-derived hydrogel and photosynthetic nano-units for myocardial infarction therapyNature communicationsAugust 18, 2025Lv Y, Zhu D, Li J, Li Y, Lu C, Bi J, Huang K, Hu S, Liu S, Yan N, et al.concentrations were measured using the ATP Detection Assay Kit (Abcam, ab113849) and the NADPH Assay Kit (Abcam, ab186031), respectively. To specifically inhibit ATP production capacity, NCU solution was pre-treated with either

(3) Curcumin-Induced Apoptotic Cell Death in Human Glioma Cells Is Enhanced by Clusterin DeficiencyPharmaceuticsMay 22, 2025Pinky Sultana, Jiri Novotnyconducted in triplicate. 2.10. NADPH Assay NADPH levels were measured using the NADPH assay kit (ab186031, Abcam, Cambridge, UK). Cells transfected with siCLU or siNC in 6-well plates were incubated for 72

(4) Progesterone increases metabolism via the pentose phosphate pathway in bovine uterine epithelial cellsMolecular Biology ReportsDecember 1, 2024Malia D. Berg, Camila U. Braz, Matthew Deansamples were stored at -20 °C. The level of NADPH in each sample was determined with an NADPH assay kit (ab186031, Abcam) per manufacturer instructions. Standards and samples (50 µl each) were added to a 96-well plate

(5) Glucose transporter 1 is essential for the resolution of methicillin-resistant S. aureus skin and soft tissue infectionsCell reportsJuly 23, 2024Banerjee SK, Thurlow LR, Kannan K, Richardson AR.assays,Critical commercial assays,Critical commercial assays ,, NADH and NADPH Assay Kit (Colorimetric),Abcam,ab186031 Luminescent ATP Detection Assay Kit,Abcam,ab113849 Cell Viability Assay Kit,Abcam,ab176748 Greiss

(6) Capsaicin pretreatment attenuates salt-sensitive hypertension by alleviating AMPK/Akt/Nrf2 pathway in hypothalamic paraventricular nucleusFrontiers in NeuroscienceMay 30, 2024Xiu-Yue Jia, Yu Yang, Xiao-Tao Jia, Da-Li Jiang, Li-Yan Fu, Hua Tian, Xin-Yan Yang, Xin-Yue Zhao, Kai-Li Liu, Yu-Ming Kang, et al.sequences used for gene expression analysis are listed in Table 1. 2.10 ELISA assay The activity of NOX (ab186031, Abcam, Cambridge, United Kingdom) in the PVN were measured with the ELISA kits. 2.11 Western blotting The

(7) Autofluorescence is a biomarker of neural stem cell activation stateCell Stem CellApril 4, 2024Christopher S. Morrow, Kelsey Tweed, Sabina Farhadova, Alex J. Walsh, Bo P. Lear, Avtar Roopra, Ryan D. Risgaard, Payton C. Klosa, Zachary P. Arndt, Ella R. Peterson, et al.Assay Abcam’s NADPH Quantification kit was used to measure NADPH levels in qNSCs and aNSCs (Abcam ab186031) following the manufacturer’s protocols. Briefly, cells were pelleted by centrifugation and then lysed

(8) Measuring the rate of NADPH consumption by glutathione reductase in the cytosol and mitochondriaPloS oneJanuary 1, 2024Ting KKY, Floro E, Dow R, Jongstra-Bilen J, Cybulsky MI, Rocheleau JV.in S2 File with this article. Quantification of total NADPH levels Total NADPH assay kits (Abcam, #ab186031) were used. In brief, cells were cultured in 6-well plates, then loaded with oxLDL for 24 h with subsequent

(9) Oxidized Low-Density Lipoprotein Accumulation Suppresses Glycolysis and Attenuates the Macrophage Inflammatory Response by Diverting Transcription from the HIF-1α to the Nrf2 PathwayJournal of immunology (Baltimore, Md. : 1950)November 15, 2023Ting KKY, Yu P, Dow R, Floro E, Ibrahim H, Scipione CA, Hyduk SJ, Polenz CK, Zaslaver O, Karmaus PWF, et al.and 590/9). Quantification of total NADPH, NADP+ and glutathione. Total NADPH assay kit (Abcam, #ab186031) and total NADP+ and NADPH assay kits (Abcam #ab186033) were used. To measure glutathione, glutathione

(10) Inhibition of 6-Phosphogluconate Dehydrogenase Reverses Epirubicin Resistance Through Metabolic Reprograming in Triple-Negative Breast Cancer CellsTechnol Cancer Res TreatAugust 10, 2023Jiali Xu, Guosheng Ren, Qiao Chengutilized to measure the synthesis of cellular lactate, while NADPH colorimetric quantification kits (ab186031, Abcam, UK) were applied to assess NADPH's level. All tests were conducted precisely in accordance with

(11) Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4bioRxivJuly 15, 2023Evangelia Chavdoula, Vollter Anastas, Alessandro La Ferlita, Julian Aldana, Giuseppe Carota, Mariarita Spampinato, Burak Soysal, Ilaria Cosentini, Sameer Parashar, Anuvrat Sircar, et al.experiments were performed in triplicates. NADPH was measured using a Colorimetric Assay Kit (Abcam, Cat. ab186031) and following the manufacturer’s protocol. Briefly, lysates derived from 1.5–2×106 cells were incubated

(12) Opioids Alleviate Oxidative Stress via the Nrf2/HO-1 Pathway in LPS-Stimulated MicrogliaInternational Journal of Molecular SciencesJuly 4, 2023Akash Shivling Mali, Ondrej Honc, Lucie Hejnova, Jiri NovotnyBioTek Synergy HT microplate reader. 4.8. NADPH Assay NADPH was measured using the NADPH assay kit (ab186031, Abcam, Cambridge, UK). C8-B4 cells grown in 12-well plates were treated with opioid ligands for 1 h

(13) Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and NeurotransmittersNutrientsOctober 7, 2022Qi J, Fu LY, Liu KL, Li RJ, Qiao JA, Yu XJ, Yu JY, Li Y, Feng ZP, Yi QY, et al.Enzyme-Linked Immunosorbent Assay (ELISA) Levels of plasma NE (H096, Nanjing Jiancheng, Nanjing, China), NOX (ab186031, Abcam, Cambridge, UK), and NF-κB p65 (R0674c, elabscience, Wuhan, China) in rat PVN were quantitatively

(14) Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancerNature communicationsJuly 26, 2022Bartolacci C, Andreani C, Vale G, Berto S, Melegari M, Crouch AC, Baluya DL, Kemble G, Hodges K, Starrett J, et al.lysates of cell lines or A549 xenografts was determined using a colorimetric NADPH Assay Kit (Abcam’s, ab186031), following the manufacturer’s instructions on LC cells and A549 xenografts treated with either FASNi

(15) Na(+)/K(+)-ATPase Alpha 2 Isoform Elicits Rac1-Dependent Oxidative Stress and TLR4-Induced Inflammation in the Hypothalamic Paraventricular Nucleus in High Salt-Induced HypertensionAntioxidants (Basel, Switzerland)January 31, 2022Su Q, Yu XJ, Wang XM, Peng B, Bai J, Li HB, Li Y, Xia WJ, Fu LY, Liu KL, et al.Diego, CA, USA), ADP/ATP ratio (MAK135-1KT, Sigma-Aldrich, Burlington, VT, USA), NAD(P)H activity (ab186031, Abcam, Cambridge, UK), NF-κB activity (ABIN6958236, Antibodyies, Limerick, ME, USA), and SOD activity

(16) Bilateral Paraventricular Nucleus Upregulation of Extracellular Superoxide Dismutase Decreases Blood Pressure by Regulation of the NLRP3 and Neurotransmitters in Salt-Induced Hypertensive RatsFrontiers in pharmacologyJanuary 1, 2021Su Q, Yu XJ, Wang XM, Li HB, Li Y, Bai J, Qi J, Zhang N, Liu KL, Zhang Y, et al.relevant indicators, including superoxide dismutase activity (Ec-SOD, ab277415; Abcam), NAD(P)H oxidase (ab186031; Abcam), malondialdehyde (MDA, S0131; Beyotime, China), and glutathione (GSH, A006-2-1; Nanjing Jiancheng

(17) Dibenzoylthiamine Has Powerful Antioxidant and Anti-Inflammatory Properties in Cultured Cells and in Mouse Models of Stress and NeurodegenerationBiomedicinesSeptember 18, 2020Sambon M, Gorlova A, Demelenne A, Alhama-Riba J, Coumans B, Lakaye B, Wins P, Fillet M, Anthony DC, Strekalova T, et al.normalized with protein assay (BCA assay). 2.9. NADPH Assay NADPH was measured using the NADPH assay kit (ab186031, Abcam, Cambridge, UK). Neuro2a cells were grown in 6-well plates. The cells were treated with 50 µM

(18) Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4Molecular medicine reportsAugust 1, 2020Wang J, Liu G.Nitric Oxide Synthase Activity assay kit (colorimetric; cat. no. ab211083; Abcam) and NADPH assay kit (colorimetric; cat. no. ab186031; Abcam), respectively. Briefly, cells in the different groups were lysed in lysis buffer (Beyotime Institute

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NADH and NADPH Assay Kit (Colorimetric) from Abcam

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NADH and NADPH Assay Kit (Colorimetric) from Abcam

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