Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 from Abcam

Product Specs
ItemAnnexin V-FITC Apoptosis Staining / Detection Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab14085
Quantity100 Test
Sample TypeAdherent cells, Suspension cells
Assay Time0h 10m
TypeStain
Gene NameANXA5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Research AreaOncology
NCBI Full Gene Nameannexin A5

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) TAK1 is a key regulator of oncogenic signaling and differentiation blockade in rhabdomyosarcomaOncogeneMay 23, 2026Anh Tuan Vuong, Aniket S. Joshi, Anirban Roy, Kavya Mathukumalli, Phuong T. Ho, Raksha Bhat, Meiricris Tomaz da Silva, Tagari Samanta, Meghana V. Trivedi, Bin Guo, et al.(PI) staining followed by FACS analysis using the Annexin V-FITC Apoptosis Staining/Detection Kit (ab14085, Abcam, USA) following a protocol suggested by the manufacturer. Transwell migration and invasion assay For

(2) Oxidative DNA lesions destabilize centromeres and drive chromosome instabilitybioRxivMay 1, 2026Lily Thompson, Rim Nassar, Annapaola Angrisani, Owen Ou Ning Hsiu, Yian Yang, Katarzina M Kedziora, Daniela Muoio, Daniele Fachinetti, Wayne Stallaert, Elise FouquerelFlowJo. Annexin V and PI staining was performed per the Annexin V-FITC Apoptosis Staining Detection Kit (Abcam #ab14085) instructions. Briefly, after collection, 5uL of Annexin-FITC and 5uL of PI per 500uL staining buffer

(3) Exploring the anti-cancer potential of gut microbiota-derived short-chain fatty acids in ovarian cancer: a comparative analysis of sodium butyrate and sodium propionate on proliferation, cell cycle, and apoptosisCancer Cell InternationalApril 17, 2026Daniella Danaf, Louna Karam, Reem Sleem, Abed El Rahman Naamani, Eliana Eldawra, José-Noel Ibrahimaccording to the manufacturer’s instructions using the Annexin V-FITC Apoptosis Detection Kit (Abcam, ab14085). Samples were analyzed using a BD Accuri™ C6 Flow Cytometer by plotting FL1-H (Annexin) against FL2-H

(4) C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCCRedox BiolApril 16, 2026Ishrat Nourin Khan, Krishna Awasthi, Ziyu Wang, Nafis Md Irfan, Jay Saepoo, Joan N. Whittier, Nurgul Koyuncu, Md Roman Mogal, M.M. Hasibuzzaman, Shujie Yang, et al.Apoptosis assays Cellular apoptosis was evaluated using the Annexin V-FITC Apoptosis Detection Kit (ab14085, Abcam) according to the manufacturer's guidelines. IL-1α-overexpressing Cal27 cells were treated with

(5) Preclinical evaluation of CPL423: a novel potent small-molecule inhibitor of TAM family and FLT3 kinase for cancer therapyFrontiers in PharmacologyMarch 25, 2026Agata Mikołajczyk, Delfina Popiel, Kinga Jastrzębska, Bartosz Wiernicki, Filip Mituła, Artur Janusz, Jakub Dominowski, Michał Górka, Tomasz Kornatowski, Joanna Hucz-Kalitowska, et al.Annexin V binding buffer (BD Pharmingen, 556454), and stained with Annexin V-FITC (1:100 dilution, Abcam, ab14085) and DRAQ7 (1:1000 dilution). After a 15-min incubation at room temperature in the dark, samples were

(6) A GasPak-Based Ischemia Model for Studying ER Stress-Ischemia Interactions in Human Endothelial CellsMethods ProtocMarch 4, 2026Mathilde Hoareau, Grégorie Lebeau, Luce Muzi, Jérémy Fontaine, Pascale Krejbich-Trotot, Olivier Meilhac, Christine Robert-Da Silva, Wildriss Viranaickenstained with Annexin V and Propidium iodide (PI) using the Annexin V-FITC Apoptosis Detection Kit (Ab14085, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Following 8 h OGD, cells were washed

(7) Upregulation of itchy E3 ubiquitin protein ligase contributes to endometrial cancer through promoting forkhead box P1 degradationCytojournalMarch 3, 2026Yong-Hong Zhang, Hai-Yang Yu, Chun-Fang Li, Jian-Chao Li106 cells/mL. Add 1 μL fluorescein isothiocyanate-labeled annexin V (Annexin V-FITC) Conjugate (Cat. ab14085, Abcam, Cambridge, MA, USA) and 12.5 μL propidium iodide solution (Cat. ab14085, Abcam, Cambridge, MA,

(8) Upregulation of itchy E3 ubiquitin protein ligase contributes to endometrial cancer through promoting forkhead box P1 degradationCytojournalMarch 3, 2026Yong-Hong Zhang, Hai-Yang Yu, Chun-Fang Li, Jian-Chao Li106 cells/mL. Add 1 μL fluorescein isothiocyanate-labeled annexin V (Annexin V-FITC) Conjugate (Cat. ab14085, Abcam, Cambridge, MA, USA) and 12.5 μL propidium iodide solution (Cat. ab14085, Abcam, Cambridge, MA,

(9) PRC2 loss impairs small cell lung cancer tumorigenesis and enhances sensitivity to G9a/GLP inhibitionCommunications BiologyFebruary 21, 2026Jawahar Kopparam, Gayathri Chandrasekaran, Danielle Hulsman, Liesa-Marie Pilger, Karen Stevers, Ji-Ying Song, Miguel Hernandez-Quiles, Jelmer Dijkstra, Jitendra Badhai, Nick Landman, et al.plates. After treatment, cells were washed and collected in 500ul of Annexin V binding buffer (Abcam, ab14085). 5 μl of FITC bound Annexin V and PI obtained from the kit were added and incubated in dark for 5 min.

(10) Synthesis, Structural Studies, and Biological Evaluation of Copper(I) and Copper(II) Complexes Supported by Bis(pyrazol-1-yl)acetate Ligand Functionalized with Amantadine for the Treatment of GlioblastomaInt J Mol SciFebruary 4, 2026Sofia Migani, Giuseppina Bozzuto, Annarica Calcabrini, Marisa Colone, Maria Luisa Dupuis, Miriam Caviglia, Cristina Aguzzi, Maria Beatrice Morelli, Fabio Del Bello, Wilma Quaglia, et al.(PI) detection kit according to the manufacturer’s protocol (Annexin V-FITC Apoptosis Detection Kit, AB14085, Abcam, Cambridge, UK). U87 MG and LN18 cell lines were seeded in 6-well plates, as described above.

(11) Multiphysics‐Driven Assembly of Biomimetic VesiclesAdvanced MaterialsJanuary 27, 2026Timofei Solodko, Ian Gimino, Aastha Chandiwala, Bayan Alkotoub, Ali Bashiri Dezfouli, Heiko Reith, Asjad Bakhtiar, Rojda Hicsanmaz, Johann Brenner, Christoph J. O. Kaiser, et al.the manufacturer's protocol, cell death was assessed using an Annexin V/PI apoptosis assay (Abcam, ab14085). U87 target cells (2 × 105 cells/well) were co‐incubated (8 h, 37°C with 5% CO2) with effector NK cells

(12) Host‐Directed Antiviral Activity of SB2960 Through Selective Induction and Remodeling of Stress GranulesAdvanced ScienceJanuary 14, 2026Wan Gi Byun, Sumin Son, JinAh Lee, Minha Lee, Meehyun Ko, Songrui Zhao, Ju Hee Kim, Kannan Vaithegi, Ji Hoon Kwon, Jung Ho Lee, et al.apoptotic and necrotic cell death using the Annexin V‐FITC Apoptosis Staining/Detection Kit [Abcam, ab14085]. Flow cytometry was conducted using a CytoFLEX LX [Beckman Coulter] equipped with laser lines at 405,

(13) Interplay between different cytotoxic parameters in Galleria mellonella (Lepidoptera, Pyralidae) larvae fed with polypropyleneScientific ReportsJanuary 7, 2026P. Mermer, Ł. Chajec, G. Wilczek, S. Student, A. K. Wrońska, J. Homa, K. Krpec, K. Magiera, D. Siwek, M. Rost-Roszkowskaperformed with the Annexin V-FITC (fluorescein isothiocyanate) Apoptosis Detection Kit (Abcam, No. ab14085) according to the manufacturer’s protocols. Apoptotic cells, after staining with the FITC-labeled Annexin

(14) Red Blood Cell Glycation Triggers In Vivo Cerebral Erythrophagocytosis in Adult Zebrafish in a Model Mimicking Hemorrhagic StrokeCompr PhysiolJanuary 6, 2026Elena Morello, Philippe Rondeau, François Canonne‐Hergaux, Emmanuel Bourdon, Matthieu Bringart, Olivier Meilhac, David Couret, Nicolas DiotelμL of RBCs resuspended at 0.4% hematocrit were incubated with 2 μg/mL of Annexin V‐FITC (Abcam, REF: ab14085) in 100 μL binding buffer for 30 min at room temperature, before flow cytometry analysis. Annexin V

(15) Preclinical Evaluation of the Efficacy of the Cyclin-Dependent Kinase Inhibitor Ribociclib in Combination with Letrozole against Patient-Derived Glioblastoma CellsMolecular Cancer TherapeuticsJanuary 2, 2026Sidharth N. Gadgil, Aniruddha S. Karve, Gary A. Gudelsky, Bhavesh B. Gabani, Shravani P. Medvedovic, Shravani P. Kulkarni, Timothy N Medvedovic, David R. Plas, Trisha M. Wise-Draper, Soma Sengupta, et al.death induced by apoptosis was assessed using the Annexin V-FITC PI Apoptosis Staining / Detection Kit (ab14085, Abcam Incorporated, Cambridge, MA) according to manufacturer’s instructions. Briefly, 1 * 105 cells/well

(16) Preclinical Evaluation of the Efficacy of a Cyclin-dependent Kinase Inhibitor Ribociclib in Combination with Letrozole Against Patient-Derived Glioblastoma CellsMol Cancer TherJanuary 2, 2026Sidharth N. Gadgil, Aniruddha S. Karve, Gary A. Gudelsky, Bhavesh B. Gabani, Mario Medvedovic, Shravani P. Kulkarni, Timothy N Phoenix, David R. Plas, Trisha M. Wise-Draper, Soma Sengupta, et al.death induced by apoptosis was assessed using the Annexin V-FITC PI Apoptosis Staining / Detection Kit (ab14085, Abcam Incorporated, Cambridge, MA) according to manufacturer’s instructions. Briefly, 1 * 105 cells/well

(17) LEM4 interacts with β-catenin and promotes β-catenin-dependent transcription activity to confers ribociclib resistance in breast cancer cellsSci RepDecember 24, 2025Qi Tang, Xintong Zhao, Hui Li, Jiaqian Liao, Bingyu Ouyang, Chunxiao Ma, Dedian Chenby flow cytometry Apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Abcam, ab14085, Cambridge, MA, USA) according to the manufacturer’s protocol. Cells were seeded at a density of 2 ×

(18) Development of a Synthetic Lethality‐Based Combination Therapy Using LIG1 and PARP Inhibitors for Prostate CancerCancer ScienceDecember 1, 2025Masaru Tani, Koji Hatano, Yu Ishizuya, Toshiki Oka, Tomohiro Kanaki, Shunsuke Inoguchi, Akihiro Yoshimura, Yuki Horibe, Yutong Liu, Sassi Nesrine, et al.of culture, cells were collected, stained with an Annexin V‐FITC Apoptosis Staining/Detection Kit (ab14085; Abcam, Cambridge, UK), and detected by flow cytometry. 2.12 Immunofluorescence DU145 and 22Rv1 cells

(19) p16 expression confers sensitivity to CDK2 inhibitors in cyclin E1–driven ovarian cancersScience SignalingNovember 25, 2025Chance C. Sine, Lotte P. Watts, Brianna Fernandez, Nasreen Marikar, Jianxin Wang, Erik S. Knudsen, Agnieszka K. Witkiewicz, Sabrina L. Spencerwas assessed using the annexin V–fluorescein isothiocyanate (FITC) apoptosis detection kit (Abcam, ab14085) according to the manufacturer’s instructions. Cells were treated with the indicated compounds for 3

(20) Combined DNA-PK and PARP Inhibition as a Therapeutic Strategy in BRCA-Mutated Prostate Cancer: An in Vitro Pilot StudyTechnology in Cancer Research & TreatmentNovember 14, 2025Thomas Paul Scherer, Souzan Salemi, Valentin Baumgartner, Dominik Enderlin, Alekhya Mazumdar, Daniel Eberlilines by flow cytometry using the Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (ab14085, Abcam, Cambridge, UK) according to the manufacturer's protocol. Briefly, 200 µL of Annexin-binding

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(1)

4/12/2023Excellent Annexin V-FITC Apoptosis Staining / Detection Kit Matteo MassaroUsed this kit to evaluate apoptosis in neuronal cells after incubation with amyloid beta 42. The kit contains propidium iodide as well for staining dead cells. Analysis was performed by flow cytometry... Read Review
(1)

3/29/2023Easy to Use Annexin V Apoptosis Staining Kit Hannah LiuI used this Annexin V-FITC Apoptosis Staining / Detection Kit in several of my projects to detect cell apoptosis or necrosis. The kit worked well and is reliable.

Annexin V-FITC Apoptosis Staining / Detection Kit from Abcam

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Annexin V-FITC Apoptosis Staining / Detection Kit from Abcam

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