Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 from Abcam

Product Specs
ItemAnnexin V-FITC Apoptosis Staining / Detection Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab14085
TypeStain
Gene NameANXA5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Research AreaOncology
NCBI Full Gene Nameannexin A5
Assay Time0h 10m
Sample TypeAdherent cells, Suspension cells
Quantity100 Test

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Neutrophils Promote Metabolic Dysfunction-Associated Steatotic Liver Disease Through Extracellular Vesicle-mediated Lipid TransferJ Extracell VesiclesAugust 3, 2026Sanjeeb Shrestha, Soo-Jung Jung, Seong-Min Lim, Yu-Bin Lee, Hyeung-Wook Shin, Hoyul Lee, Hee-Kyung Jin, Jae-Sung Bae, Jae-Han Jeon, Lai Guan Ng, et al.neutrophils (1 × 106 cells) for 2 h were stained using Annexin V‐FITC apoptosis detection kit (Abcam, #ab14085). Flow cytometric analysis for apoptotic rates was examined using BD FACSCalibur flow cytometer (BD

(2) SIRT2 antagonizes MOF function during mitotic entrySci AdvJune 19, 2026María Espinosa-Alcantud, Núria Sima, Irene Fernández-Duran, Anna Marazuela-Duque, Gerard Martínez-Cebrián, Anna Guitart-Solanes, Andrés Gámez-García, Paloma Martínez-Redondo, Laia Bosch-Presegué, Berta N. Vazquez, et al.software. For apoptosis assay, we used the Annexin V-FITC Apoptosis Staining/Detection Kit (Abcam, ab14085). Briefly, 105 cells were washed and resuspended in binding buffer in the presence of annexin V–fluorescein

(3) Carborane Hydrophobic Tags Drive Selective Degradation of Endogenous KRAS G12C via HSP70–Ubiquitin–Proteasome PathwayACS Bio & Med Chem AuJune 17, 2026Yujie Shao, Kazuki Miura, Hiroyuki Nakamuracompounds for different time. Then, the cells were collected and stained by Annexin V-FITC/PI kit (#ab14085, Abcam Ltd., UK) according to the manufacturer’s instructions. The fluorescence signals of FITC and

(4) miR-211-5p/FOXO3 axis accelerates osteogenic differentiation and fracture healing by mediating Wnt/β-catenin pathwayJournal of Orthopaedic Surgery and ResearchJune 1, 2026Huifei Zhai, Mianlong Lin, Changchun Lu, Jixia LiangAnnexin V-FITC and PI staining solutions (5 μL; Abcam; Annexin V-FITC/PI Apoptosis Detection Kit, Cat#ab14085, Lot#GR256892-1). Incubate at room temperature in the dark for 15 min, then stop the reaction by placing

(5) Tumor Treating Fields modulate apoptotic and immune programs in T‑cell acute lymphoblastic leukemia cell linesExperimental and Therapeutic MedicineMay 29, 2026Jinju Heo, Myonggeun YoonLLC). Apoptosis assay Apoptosis was evaluated using the Annexin V-FITC/PI Apoptosis Detection Kit (cat. no. ab14085; Abcam). Following TTFields treatment, cells were washed with cold PBS and resuspended in binding buffer.

(6) Comprehensive Analysis of Genomic and Phenomic Data Reveals Context-Dependent Function of A20 (TNFAIP3) in Renal Cell CarcinomaCancers (Basel)May 28, 2026Nour Abu Jayab, Burcu Yener, Reem Sami Alhamidi, Mansi Bhavsar, Alaa Muayad Altaie, Muna Abdalla Alhammadi, Vidya Bijosh Mohan, Marwa Khamis Almazrouei, Lina Sahnoon, Rola Abujabal, et al.was assessed using the Annexin V-Fluorescein isothiocyanate (FITC) Apoptosis Detection Kit (Abcam, ab14085) according to the manufacturer’s instructions. Briefly, a master staining solution containing Annexin

(7) TAK1 is a key regulator of oncogenic signaling and differentiation blockade in rhabdomyosarcomaOncogeneMay 23, 2026Anh Tuan Vuong, Aniket S. Joshi, Anirban Roy, Kavya Mathukumalli, Phuong T. Ho, Raksha Bhat, Meiricris Tomaz da Silva, Tagari Samanta, Meghana V. Trivedi, Bin Guo, et al.(PI) staining followed by FACS analysis using the Annexin V-FITC Apoptosis Staining/Detection Kit (ab14085, Abcam, USA) following a protocol suggested by the manufacturer. Transwell migration and invasion assay For

(8) Phenotypic Response Surfaces–Guided Optimization (PRS-OPT) of Propolis-Metformin-Regorafenib Combination Therapy for MASLD-Associated Hepatocellular CarcinomaOncology ResearchMay 21, 2026Yi-Sian Huang, Chung-Yung Ma, Hsiao-Yuh Roan, Cheng-Hsiung Chiang, Hsiao-Hui Tsou, Chen-Hui Chen, Yi-Fan Lin, Horng-Dar Wang, Chiou-Hwa Yuhcells/well). Apoptosis was quantified by Annexin V-FITC/PI staining using an apoptosis detection kit (Abcam ab14085, Cambridge, UK) after 48 h treatment under defined conditions: (1) vehicle control (BSA/ethanol-matched),

(9) Oxidative Stress Drives Cell Cycle Stalling, Apoptosis and Metabolic Suppression in Cystatin B Deficient EPM1 Patient iPSCsCell ProliferationMay 19, 2026Shekhar Singh, Lidiia Plotnikova, Teemu Tiukuvaara, Reetta Kälviäinen, Riikka H. Hämäläinenprogramme. 2.8 Apoptosis Assay Apoptosis was assessed using the Annexin V‐FITC Apoptosis Detection Kit (Abcam, ab14085) according to the manufacturer's instructions. Samples were analysed immediately using a CytoFLEX S

(10) Oxidative DNA lesions destabilize centromeres and drive chromosome instabilitybioRxivMay 1, 2026Lily Thompson, Rim Nassar, Annapaola Angrisani, Owen Ou Ning Hsiu, Yian Yang, Katarzina M Kedziora, Daniela Muoio, Daniele Fachinetti, Wayne Stallaert, Elise FouquerelFlowJo. Annexin V and PI staining was performed per the Annexin V-FITC Apoptosis Staining Detection Kit (Abcam #ab14085) instructions. Briefly, after collection, 5uL of Annexin-FITC and 5uL of PI per 500uL staining buffer

(11) Exploring the anti-cancer potential of gut microbiota-derived short-chain fatty acids in ovarian cancer: a comparative analysis of sodium butyrate and sodium propionate on proliferation, cell cycle, and apoptosisCancer Cell InternationalApril 17, 2026Daniella Danaf, Louna Karam, Reem Sleem, Abed El Rahman Naamani, Eliana Eldawra, José-Noel Ibrahimaccording to the manufacturer’s instructions using the Annexin V-FITC Apoptosis Detection Kit (Abcam, ab14085). Samples were analyzed using a BD Accuri™ C6 Flow Cytometer by plotting FL1-H (Annexin) against FL2-H

(12) C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCCRedox BiolApril 16, 2026Ishrat Nourin Khan, Krishna Awasthi, Ziyu Wang, Nafis Md Irfan, Jay Saepoo, Joan N. Whittier, Nurgul Koyuncu, Md Roman Mogal, M.M. Hasibuzzaman, Shujie Yang, et al.Apoptosis assays Cellular apoptosis was evaluated using the Annexin V-FITC Apoptosis Detection Kit (ab14085, Abcam) according to the manufacturer's guidelines. IL-1α-overexpressing Cal27 cells were treated with

(13) Preclinical evaluation of CPL423: a novel potent small-molecule inhibitor of TAM family and FLT3 kinase for cancer therapyFrontiers in PharmacologyMarch 25, 2026Agata Mikołajczyk, Delfina Popiel, Kinga Jastrzębska, Bartosz Wiernicki, Filip Mituła, Artur Janusz, Jakub Dominowski, Michał Górka, Tomasz Kornatowski, Joanna Hucz-Kalitowska, et al.Annexin V binding buffer (BD Pharmingen, 556454), and stained with Annexin V-FITC (1:100 dilution, Abcam, ab14085) and DRAQ7 (1:1000 dilution). After a 15-min incubation at room temperature in the dark, samples were

(14) Synergistic effects of lipopolysaccharide and titanium ions on monocytic myeloid-derived suppressor cell expansion in gingival tissue: implications for peri-implant bone healthJournal of Periodontal & Implant ScienceMarch 13, 2026Eun-Young Jang, Seok Bin Yang, Jung-Mi Lee, Kyu Hwan KwackHarvested cells were washed with Dulbecco’s PBS and stained with Annexin V-FITC and propidium iodide (ab14085, Abcam, Cambridge, UK). The proportions of live and dead cells were analyzed by flow cytometry (BD Verse,

(15) A GasPak-Based Ischemia Model for Studying ER Stress-Ischemia Interactions in Human Endothelial CellsMethods ProtocMarch 4, 2026Mathilde Hoareau, Grégorie Lebeau, Luce Muzi, Jérémy Fontaine, Pascale Krejbich-Trotot, Olivier Meilhac, Christine Robert-Da Silva, Wildriss Viranaickenstained with Annexin V and Propidium iodide (PI) using the Annexin V-FITC Apoptosis Detection Kit (Ab14085, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Following 8 h OGD, cells were washed

(16) Upregulation of itchy E3 ubiquitin protein ligase contributes to endometrial cancer through promoting forkhead box P1 degradationCytojournalMarch 3, 2026Yong-Hong Zhang, Hai-Yang Yu, Chun-Fang Li, Jian-Chao Li106 cells/mL. Add 1 μL fluorescein isothiocyanate-labeled annexin V (Annexin V-FITC) Conjugate (Cat. ab14085, Abcam, Cambridge, MA, USA) and 12.5 μL propidium iodide solution (Cat. ab14085, Abcam, Cambridge, MA,

(17) Upregulation of itchy E3 ubiquitin protein ligase contributes to endometrial cancer through promoting forkhead box P1 degradationCytojournalMarch 3, 2026Yong-Hong Zhang, Hai-Yang Yu, Chun-Fang Li, Jian-Chao Li106 cells/mL. Add 1 μL fluorescein isothiocyanate-labeled annexin V (Annexin V-FITC) Conjugate (Cat. ab14085, Abcam, Cambridge, MA, USA) and 12.5 μL propidium iodide solution (Cat. ab14085, Abcam, Cambridge, MA,

(18) PRC2 loss impairs small cell lung cancer tumorigenesis and enhances sensitivity to G9a/GLP inhibitionCommunications BiologyFebruary 21, 2026Jawahar Kopparam, Gayathri Chandrasekaran, Danielle Hulsman, Liesa-Marie Pilger, Karen Stevers, Ji-Ying Song, Miguel Hernandez-Quiles, Jelmer Dijkstra, Jitendra Badhai, Nick Landman, et al.plates. After treatment, cells were washed and collected in 500ul of Annexin V binding buffer (Abcam, ab14085). 5 μl of FITC bound Annexin V and PI obtained from the kit were added and incubated in dark for 5 min.

(19) Synthesis, Structural Studies, and Biological Evaluation of Copper(I) and Copper(II) Complexes Supported by Bis(pyrazol-1-yl)acetate Ligand Functionalized with Amantadine for the Treatment of GlioblastomaInt J Mol SciFebruary 4, 2026Sofia Migani, Giuseppina Bozzuto, Annarica Calcabrini, Marisa Colone, Maria Luisa Dupuis, Miriam Caviglia, Cristina Aguzzi, Maria Beatrice Morelli, Fabio Del Bello, Wilma Quaglia, et al.(PI) detection kit according to the manufacturer’s protocol (Annexin V-FITC Apoptosis Detection Kit, AB14085, Abcam, Cambridge, UK). U87 MG and LN18 cell lines were seeded in 6-well plates, as described above.

(20) Multiphysics‐Driven Assembly of Biomimetic VesiclesAdvanced MaterialsJanuary 27, 2026Timofei Solodko, Ian Gimino, Aastha Chandiwala, Bayan Alkotoub, Ali Bashiri Dezfouli, Heiko Reith, Asjad Bakhtiar, Rojda Hicsanmaz, Johann Brenner, Christoph J. O. Kaiser, et al.the manufacturer's protocol, cell death was assessed using an Annexin V/PI apoptosis assay (Abcam, ab14085). U87 target cells (2 × 105 cells/well) were co‐incubated (8 h, 37°C with 5% CO2) with effector NK cells

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(1)

4/12/2023Excellent Annexin V-FITC Apoptosis Staining / Detection Kit Matteo MassaroUsed this kit to evaluate apoptosis in neuronal cells after incubation with amyloid beta 42. The kit contains propidium iodide as well for staining dead cells. Analysis was performed by flow cytometry... Read Review
(1)

3/29/2023Easy to Use Annexin V Apoptosis Staining Kit Hannah LiuI used this Annexin V-FITC Apoptosis Staining / Detection Kit in several of my projects to detect cell apoptosis or necrosis. The kit worked well and is reliable.

Annexin V-FITC Apoptosis Staining / Detection Kit from Abcam

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Annexin V-FITC Apoptosis Staining / Detection Kit from Abcam

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