Bradford Assay Kit ab102535 from Abcam

Product Specs
ItemBradford Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab102535
Quantity1000 Test
Sample TypeSerum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
Assay Time0h 20m

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Ovarian expression of functional MTTP and apoB for VLDL assembly and secretion in chickensPoult SciMarch 6, 2025Yu-Hui Chen, Petnamnueng Dettipponpong, Mei-Ying Sin, Ling-Chu Chang, Chuen-Yu Cheng, San-Yuan Huang, Rosemary L. Walzem, Hsu-Chen Cheng, Shuen-Ei ChenCusabio, Houston, TX, USA). Total protein content was determined by Bradford assay with a commercial kit (ab102535, Abcam). Statistics Data was analyzed by one-way ANOVA followed by Student's t-test or Tukey multiple

(2) Metformin effectively alleviates the symptoms of Alzheimer in rats by lowering amyloid β deposition and enhancing the insulin signalMetabolic Brain DiseaseNovember 25, 2024Hamed A. Abosharaf, Yasmin Elsonbaty, Ehab Tousson, Tarek M. Mohamed2021). Immunoblotting analysis Protein concentration was determined using a Bradford assay Abcam kit (Cat. No. ab102535), and protein was extracted from hippocampus tissues using a specific protein extraction kit set from

(3) Durability of the moderate-to-heavy-intensity transition is related to the effects of prolonged exercise on severe-intensity performanceEuropean Journal of Applied PhysiologyAugust 1, 2024Kate Hamilton, Andrew E. Kilding, Daniel J. Plews, Mathew J. Mildenhall, Mark Waldron, Thanchanok Charoensap, Tobias H. Cox, Matthew J. Brick, Warren B. Leigh, Ed Maunderexpressed relative to sample protein concentration using a Bradford assay, performed in triplicate (ab102535, Abcam®; CV, 6.1%). Carnosine concentrations, as assessed by 1H-magnetic resonance spectroscopy, have

(4) Renoprotective effect of a novel combination of 6-gingerol and metformin in high-fat diet/streptozotocin-induced diabetic nephropathy in rats via targeting miRNA-146a, miRNA-223, TLR4/TRAF6/NLRP3 inflammasome pathway and HIF-1αBiological ResearchJuly 20, 2024Merna G. Aboismaiel, Mohamed N. Amin, Laila A. Eissathe tissue’s protein content [48] using a Bradford Assay Kit (Abcam, Waltham, MA, USA; Catalog No. ab102535). Assay of oxidative stress and lipid peroxidation biomarkers Spectrophotometric assay kits from Biodiagnostic

(5) Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal musclePflügers Archiv - European Journal of PhysiologyJune 1, 2024Ed Maunder, Andrew King, Jeffrey A. Rothschild, Matthew J. Brick, Warren B. Leigh, Christopher P. Hedges, Troy L. Merry, Andrew E. Kildingduplicate. Enzyme activities were expressed relative to sample protein concentration using a Bradford assay (ab102535, Abcam®; CV, 6.1%). Assays were performed on a spectrophotometer (Multiskan GO, ThermoFisher Scientific

(6) Morphological and cytoskeleton changes in cells after EMTSci RepDecember 13, 2023Assel Nurmagambetova, Vadim Mustyatsa, Aleena Saidova, Ivan VorobjevWestern blot detection Cells were lysed in RIPA buffer for 30 min. Protein concentration was measured using Bradford assay (ab102535; Abcam). Protein lysates were resolved in 12% SDS‐PAGE gel and transferred to 0.4 μm Immobilon-P PVDF

(7) Skeletal muscle proteins involved in fatty acid transport influence fatty acid oxidation rates observed during exercisePflügers Archiv - European Journal of PhysiologyJuly 18, 2023Ed Maunder, Jeffrey A. Rothschild, Andreas M. Fritzen, Andreas B. Jordy, Bente Kiens, Matthew J. Brick, Warren B. Leigh, Wee-Leong Chang, Andrew E. Kildingto protein standards and samples, and optical density was measured on a spectrophotometer at 595 nm (ab102535, Abcam®). Abundances of CD36 (ab267614, Abcam®), FATP1 (MBS456856, MyBioSource), FATP4 (MBS456858, MyBioSource),

(8) Inositol Alleviates Pulmonary Fibrosis by Promoting Autophagy via Inhibiting the HIF-1α-SLUG Axis in Acute Respiratory Distress SyndromeOxidative Medicine and Cellular LongevityDecember 23, 2022Yufeng Liang, Yingyi Xu, Bingtai Lu, Yuanming Huang, Shuman Xu, Junjie Xie, Ming Liu, Di Che, Liuheyi Ma, Jianping Tao, et al.China), and the protein concentration in each extract was determined using a Bradford protein assay kit (ab102535, Abcam, Cambridge, UK. Next, a 20 ug sample of total protein from each extract was separated by SDS-PAGE

(9) miR-642a-5p increases glucocorticoid sensitivity by suppressing the TLR4 signalling pathway in THP-1 cellsBiochemistry and biophysics reportsDecember 1, 2022Luo J, Wang Y, Dong X, Wang W, Mu Y, Sun Y, Zhang F, Miao Y.as described by Adibki et al. [20]. The protein content was quantified using a Bradford Assay Kit (ab102535, Abcam). SDS–PAGE gels (Tris-Gly, 4–20%, Beyotime) were used to separate the proteins, and samples containing

(10) Bcl-xL activity influences outcome of the mitotic arrestFrontiers in PharmacologySeptember 15, 2022M. Suleimenov, S. Bekbayev, M. Ten, N. Suleimenova, M. Tlegenova, A. Nurmagambetova, S. Kauanova, I. Vorobjevcentrifuged for 2 min at 10,000 rpm. For protein quantification, a colorimetric Bradford assay kit (ab102535, Abcam, United States) was used. The total protein was separated using 12% sodium dodecyl sulfate–polyacrylamide

(11) Effect of Chronic Treatment with Uridine on Cardiac Mitochondrial Dysfunction in the C57BL/6 Mouse Model of High-Fat Diet–Streptozotocin-Induced DiabetesInternational Journal of Molecular SciencesSeptember 13, 2022Natalia V. Belosludtseva, Vlada S. Starinets, Irina B. Mikheeva, Maxim N. Belosludtsev, Mikhail V. Dubinin, Galina D. Mironova, Konstantin N. Belosludtsevsuspensions contained 15–30 mg of mitochondrial protein/mL, as determined using a Bradford Assay Kit (ab102535, Abcam, Cambridge, UK). The rate of O2 consumption by isolated mitochondria was estimated by high-resolution

(12) Intermittent Lighting Program Relieves the Deleterious Effect of Heat Stress on Growth, Stress Biomarkers, Physiological Status, and Immune Response of Broiler ChickensAnimals (Basel)July 19, 2022Abdulaziz A. Alaqil, Hanaa K. Abd El-Atty, Ahmed O. Abbasand chicken triiodothyronine (chT3). Briefly, plasma TP was detected using the Bradford assay kit (ab102535, Abcam, Waltham, MA, USA), which produces a blue complex detected at 595 nm. For the plasma AST and

(13) Lactobacillus improves the effects of prednisone on autoimmune hepatitis via gut microbiota-mediated follicular helper T cellsCell communication and signaling : CCSJune 3, 2022Ma L, Zhang L, Zhuang Y, Ding Y, Chen J.protease inhibitors (Beyotime Biotech). Protein concentration was quantified by a Bradford assay kit (ab102535; Abcam). Total protein (30 µg) from each sample was separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis

(14) Citrullus colocynthis Seed Ameliorates Layer Performance and Immune Response under Acute Oxidative Stress Induced by Paraquat InjectionAnimals (Basel)April 7, 2022Ahmed O. Abbas, Abdulaziz A. Alaqil, Nancy N. Kamel, Eman S. Moustafacholesterol levels were quantified according to the kits’ protocols using quantitative colorimetric assays (ab102535, ab65336, and ab65390, respectively; Abcam, Waltham, MA, USA). As for liver enzyme activity, alanine

(15) Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained malesEuropean Journal of Applied PhysiologyJanuary 1, 2022Ed Maunder, Daniel J. Plews, Gareth A. Wallis, Matthew J. Brick, Warren B. Leigh, Wee-Leong Chang, Tom Stewart, Casey M. Watkins, Andrew E. Kildingto protein standards and samples, and optical density was measured on a spectrophotometer at 595 nm (ab102535, Abcam®). Citrate synthase (CS) activity was determined via a kinetic immunocapture assay (ab119692,

(16) Mitochondrial ROS Induced Lysosomal Dysfunction and Autophagy Impairment in an Animal Model of Congenital Hereditary Endothelial DystrophyInvestigative ophthalmology & visual scienceSeptember 2, 2021Shyam R, Ogando DG, Choi M, Liton PB, Bonanno JA.following the manufacturer's instructions. Protein concentration was determined using the Bradford assay (#ab102535; Abcam). Equal amounts of cytosolic fractions (approximately 1.5% of total cytosol extract), and equal

(17) Citrullus colocynthis Seeds: A Potential Natural Immune Modulator Source for Broiler Reared under Chronic Heat StressAnimals (Basel)June 30, 2021Mohamed I. Alzarah, Fayez Althobiati, Ahmed O. Abbas, Gamal M. K. Mehaisen, Nancy N. Kamelalbumin (A), ALT, and AST concentrations were determined following the instructions of the kit manufacturers (ab102535, ab235628, ab241035, and ab105135, respectively; Abcam, MA, USA) as indicators for the broiler liver

(18) In vivo Evaluation of a Newly Synthesized Acetylcholinesterase Inhibitor in a Transgenic Drosophila Model of Alzheimer’s DiseaseFrontiers in NeuroscienceJune 30, 2021Giuseppe Uras et al.transferred into a fresh new vial. Protein concentrations were quantified using Bradford Assay Kit (#ab102535, Abcam, United Kingdom). According to protein concentrations, samples were then dissolved in 4 × NuPage

(19) Temperate performance and metabolic adaptations following endurance training performed under environmental heat stressPhysiological ReportsMay 12, 2021Ed Maunder, Daniel J. Plews, Gareth A. Wallis, Matthew J. Brick, Warren B. Leigh, Wee‐Leong Chang, Casey M. Watkins, Andrew E. Kildingto protein standards and samples, and optical density was measured on a spectrophotometer at 595 nm (ab102535, Abcam®). Maximal citrate synthase (CS) activity was measured via a kinetic immunocapture assay (ab119692,

(20) NLRX1/FUNDC1/NIPSNAP1‐2 axis regulates mitophagy and alleviates intestinal ischaemia/reperfusion injuryCell ProliferationMarch 1, 2021Shaoqin Li, Yi Zhou, Xiaocheng Gu, Xiaoping Zhang, Zhongzhi Jiaprotein extraction kit according to the manufacturer’s instruction and quantified using Bradford assay (ab102535; Abcam). Protein samples at a concentration of 25 μg were separated using 10%‐25% sodium dodecyl sulphate

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Bradford Assay Kit from Abcam

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Bradford Assay Kit from Abcam

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