Cellular ROS Assay Kit (Red) ab186027 from Abcam

Product Specs
ItemCellular ROS Assay Kit (Red)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab186027
Quantity200 Test
Sample TypeAdherent cells, Suspension cells
Assay Time0h 60m

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Website: http://www.abcam.com

(1) Roles of Oxidative Phosphorylation and Fatty Acid Oxidation in Neuroinflammation Induced by Lipopolysaccharide in Hypothalamic Neuronal CellsInt J InflamApril 15, 2026Mohsine-Ali El-Hamri, Meriem Lahmouad, Jihane Zerrouk, Rafik El-Mernissi, Lhoussain Hajji, Khayelihle Brian Makhathini, Hanane Khalki, Oualid Abboussihydroxyl radicals were detected using the Cellular Reactive Oxygen Species Detection Assay Kit (No. ab186027, Abcam, US). The control and experimental group cells were seeded into a 96‐well plate at 2 × 104 cells

(2) Differential Effects of Phenanthrene and Its Chlorinated Congeners on Hormone Production and Mitochondrial Function in Ovarian Granulosa CellsToxicsApril 7, 2026Genevieve A. Perono, Laiba Jamshed, Rohita Dutt, Reese S. Cameron, James J. Petrik, Philippe J. Thomas, Alison C. Hollowaynumber. Cellular ROS: ROS levels were measured using the Cellular Reactive Oxygen Species Detection Assay kit (ab186027, Abcam, Cambridge, UK) (N = 5–8 independent experiments). SIGCs were seeded at 12,000 cells/well in

(3) Maternal exposure to low-dose tartrazine during lactation induces neurotoxicity in rat pups through oxidative stress, endoplasmic reticulum stress, and autophagy suppressionAnatomy & Cell BiologyMarch 31, 2026Amal S. Sewelam, Bashir Jarrar, Asmaa Mohammed Tolba, Emtethal Mamdouh El-Bestawyreactive oxygen species (ROS) were assayed via assay kits (Cat # ab233471, Abcam for MDA and Cat # ab186027, Abcam for ROS). Moreover, the OxiSelectTM total antioxidant capacity (TAC) assay kit (Cat # STA-360;

(4) MK4 Repositioning for IAHSP: Overcoming In Vivo Data Gaps through In Silico Refinement and In Vitro ValidationACS Chemical NeuroscienceMarch 18, 2026Matteo Rossi Sebastiano, Antonio Vicidomini, Serena Francisco, Verdiana Pullano, Paola Defilippi, Gabriele Baj, Fabrizia Cesca, Giulia Caron, Giuseppe ErmondiSpecies Quantification ROS titration was carried out with the cellular ROS assay kit from Abcam (catalog ab186027). In brief, 5.0 × 103 cells were seeded in black, clear-bottom, 96-well plates (165305-Thermo Fisher)

(5) New N-Heterocyclic Carbene Gold and Platinum Complexes with 1,3-Dialkyl-4-anisyl-5-(4-chlorophenyl)imidazol-2-ylidene Ligands for the Treatment of Esophageal AdenocarcinomaInternational Journal of Molecular SciencesFebruary 21, 2026Hindole Ghosh, Tobias Rehm, Sangita Bhattacharyya, Miru Lee, Dileepkumar Veeragoni, Rainer Schobert, Bernhard Biersack, Prasad DandawateAfter 72 h, the wells were treated with the ROS staining kit from Abcam (Cellular ROS Assay Kit (Red), ab186027, Cambridge, MA, USA). After 37 min, readings were taken with a fluorometric plate reader (Ex/Em 520/605

(6) Synergistic therapeutic effects of metformin and curcumin on polycystic ovary syndrome via regulation of insulin resistance and oxidative stress in a rat modelFront Endocrinol (Lausanne)February 16, 2026Lina Zheng, Baohua Wu, Junli Zhang, Yianduo Zhang, Zhiling Yang, Caifeng Deng, Chaolin Huang, Ling Lumalondialdehyde (MDA), were quantified using commercial assay kits, including the ROS Detection Assay Kit (ab186027, Abcam, Shanghai, China) and the Lipid Peroxidation (MDA) Assay Kit (ab233471, Abcam), respectively.

(7) Characterization of a Bioprinted Anticancer Cell Therapy System Generated with Continuous Liquid Interface ProductionAdvanced NanoBiomed ResearchJanuary 26, 2026Lauren Kass, Ike Keku, Yu Zhang, Justin Forbes, Morrent Thang, Jillian Perry, Shawn Hingtgenreactive oxygen species (ROS) generated during bioprinting, a cellular ROS sensor was used (Abcam, ab186027) according to the manufacturer’s instructions. NHF1TRAIL cells were incubated with 1X PBS containing

(8) Combination nitazoxanide and auranofin treatment has synergistic anticancer activity in anaplastic thyroid cancer through enhanced activation of oxidative stress that leads to apoptosisCancer LettersNovember 1, 2025Chandrayee Ghosh, Tejinder Pal Khaket, Viswanath Gunda, Zhongyue Yang, Jiangnan Hu, Eden Demissie Alamaw, Lisa Zhang, Ya-Qin Zhang, Min Shen, Yasmine Tabdili, et al.assay Cellular reactive oxygen species (ROS) was measured using Abcam ROS detection assay kits (cat no# ab186027, cat no # ab113851 Abcam, Cambridge, UK).1.0 ×104 cells were plated in a black wall, clear bottom 96-well

(9) Prenatal exposure to Zika virus shapes offspring neutrophil function in a sex-specific mannerNat CommunOctober 3, 2025Jiahui Ding, Anna Hu, Annie Thy Nguyen, Grace M. Swanson, Aditi Singh, Nicholas Adzibolosu, Diana Manchorova, Elizabeth Findeis, Anthony Maxwell, Yuan He, et al.production by mouse neutrophils was quantified using the Cellular ROS Assay Kit (Red) (Abcam, Cat. No. ab186027) according to the manufacturer’s protocol. Freshly isolated mouse neutrophils (5 × 10⁴ cells in 100

(10) A sesquiterpene lactone, tomentosin, as a novel anticancer agent: orchestrating apoptosis, autophagy, and ER stress in colorectal cancerNaunyn-Schmiedeberg's Archives of PharmacologySeptember 1, 2025Sümeyra Çetinkaya, Ebru Güçlü, İlknur Çınar Ayan, Hasibe Vural, Hatice Gül Dursunalters the oxidative balance in cells, intracellular ROS levels were measured using ROS Red Dye (Cat#ab186027, Abcam). After the cells reached sufficient confluency, 100 μL of ROS red working solution was added

(11) Engineered nanofiber scaffolds prime chromatin reorganization to sensitize cells for long-term and low-dose chemical hazard detectionFrontiers in PharmacologyAugust 25, 2025Yupeng Zhang, Hang Zhao, Li Jiang, Qi Zhang, Tingbao Cao, Zesheng Wang, Yang Song, Kunpeng Quassay Both Global DNA Methylation assay and ROS assay were employed ELISA assay (Abcam, ab233486 and ab186027). Samples were added to pre-coated 96-well plates provided in the kits. The plates were incubated with

(12) Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophyNPJ Syst Biol ApplJuly 1, 2025Yu-Te Lin, Yi-Ju Lee, Wen-Wei Tseng, Zih-Hua Chen, Huai-Ching Hsieh, Ko-Hong Lin, Jin-Yu Su, An-Chi WeiAfter a 24-h incubation period, the cellular ROS levels were detected using a Cellular ROS Assay Kit (ab186027; Abcam, UK) and measured using a SpectraMax iD3 plate reader (Molecular Devices, USA). Estimating cell

(13) Cold atmospheric plasma effectively kills chordoma cells through induction of intracellular reactive oxygen speciesSci RepJuly 1, 2025Sophie Peeters, Peter B. Wu, Blake Haist, Amber Armellini, Wi Jin Kim, Zhitong Chen, Richard Obenchain, George Ayad, Weihong Ge, Aparna Bhaduri, et al.triplicate. Intracellular ROS measurement We used the cell-based quantitative Cellular ROS Assay (Abcam; ab186027). The assay uses a cell-permeable red dye that generates red fluorescence upon reacting with ROS in

(14) 5hmC enhances PARP trapping and restores PARP inhibitor sensitivity in chemoresistant BRCA1/2-deficient cellsJournal of Biological ChemistryJune 19, 2025Suhas S. Kharat, Arun P. Mishra, Satheesh K. Sengodan, Dillon Dierman, Stephen D. Fox, Walter J. Chazin, Shyam K. Sharan(Agilent Technologies). ROS Assay ROS levels were measured using a Cellular ROS Assay Kit (Abcam; #ab186027), performed according to the manufacturer’s instructions. Briefly, cells were seeded in 96-well plates

(15) Profiling the effect of low frequency mechanical vibration on the metabolic and oxidative stress responses of A431 carcinomaFEBS Open BioMay 16, 2025Wresti L. Anggayasti, Chikahiro Imashiro, Takashi Morikura, Shogo Miyata, Akira Funahashi, Kenjiro Takemuracell were measured using the Cellular Reactive Oxygen Species Detection Assay kit (Red Fluorescence) (ab186027; Abcam, UK), based on the manufacturer's instructions. The cells were imaged and analyzed using a high‐content

(16) MFGE-8, a Corona Protein on Extracellular Vesicles, Mediates Self-Renewal and Survival of Human Pluripotent Stem CellsJ Extracell VesiclesMarch 25, 2025Youngseok Lee, Hyojin Kim, Heeseok Yoon, Seunghyun Cho, Jeongjun Kim, Jihun Lee, Sang-Hun Choi, Hyesun Cho, Dong-Hun Woo, Jung-Hyuck Park, et al.cells were washed twice, incubated with ROS Red Dye working solution (Cellular ROS Assay Kit [Red], ab186027, Abcam) for 1 h at 37°C and measured with a microplate reader as fluorescence excitation/emission =

(17) Amniotic fluid stem cell conditioned medium’s role in Schwann cell proliferation, survival, and cellular antioxidant activity under normative and oxidative stress conditionsAnnals of Translational MedicineFebruary 28, 2025Chukwuweike Gwam, Nequesha Mohamed, Ayobami S. Ogunsola, Marcel G. Brown, Kaitlin A. Henry, Xue MaROS were quantified for the cell culture using the cellular ROS detection kit (Abcam, product number: ab186027) (29) according to the manufacturer’s instructions. The fluorescence signal was read by a fluorescence

(18) Attenuated growth factor signaling during cell death initiation sensitizes membranes towards peroxidationNat CommunFebruary 25, 2025André Gollowitzer, Helmut Pein, Zhigang Rao, Lorenz Waltl, Leonhard Bereuter, Konstantin Loeser, Tobias Meyer, Vajiheh Jafari, Finja Witt, René Winkler, et al.in NIH-3T3 fibroblasts exposed to cytotoxic stress were determined by a Cellular ROS Assay Kit (Red; ab186027, Abcam). Briefly, cells (1 × 104/well) were cultured at 37 °C and 5% CO2 in DMEM plus FCS (10%) for

(19) Copper-Induced Enhancement of Glioblastoma Tumorigenicity via Cytochrome C OxidaseAntioxidants (Basel)January 24, 2025Claudia R. Oliva, Md Yousuf Ali, Susanne Flor, Corinne E. Griguerpost-treatment using the Cellular Reactive Oxygen Species Detection Assay Kit (Abcam, Waltham, MA, USA. Cat No. ab186027), following the manufacturer’s protocol. Fluorescence (λ ex/em 520/605 nm) was measured using a Tecan

(20) ECM Modifications Driven by Age and Metabolic Stress Directly Promote the Vascular Smooth Muscle Cell Osteogenic ProcessesArteriosclerosis, Thrombosis, and Vascular BiologyJanuary 16, 2025Meredith Whitehead, Maria Faleeva, Rafael Oexner, Susan Cox, Lukas Schmidt, Manuel Mayr, Catherine M. ShanahanAnalysis Accumulation of reactive oxygen species (ROS) was measured using a Cellular ROS Assay Kit (Abcam; ab186027) according to the manufacturer’s instructions. N-acetylcysteine (NAC; Merck; A9165) was used at 10 μmol/L

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Cellular ROS Assay Kit (Red) from Abcam

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