Fig 1: GLP1R and PPARγ agonists are effective in decreasing ROS production induced by PCN. Confluent, submerged 16HBEo- cells were pretreated with 6 GLP1R agonists (1 µM) and 2 PPARγ agonists (10 µM) for 1 hour. Then, cells were treated with 55 µg/mL PCN (in 20 µL/well) for 1 hour, and the intracellular ROS was measured using an excitation/emission at 520/605 nm in bottom-read mode using the Cellular Reactive Oxygen Species Assay kit (ab186027, Abcam) according to the manufacturer’s instructions. (A) All 6 GLP1R agonists effectively attenuate the intracellular ROS levels induced by PCN. (B) Both PPARγ agonists lowered intracellular ROS levels induced by PCN. The experiments were performed in duplicate and independently four times. All data are presented as mean ± s.e.m. from all 4 experiments. ****p < 0.0001 as analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test.