Fig 1: Representative melanoma spheroids suitable for TRACEQC analysis(A) Representative mono-culture melanoma spheroids (WM164, WM793, WM983b).(B) Images depicting fluorescent labeling of co-culture spheroids relative to phase.Cells were stained with CytoPainter Cell Tracking Staining Kit- Deep Red and Green Fluorescence (Abcam, Cat # ab138894, ab176735). The merged image includes phase and both fluorescent channels.(C) Representative co-culture Panc10.05 + hT231 spheroids. Scale bars = 200 μm.