Fig 1: Protein expression of mitochondrial function markers in the lung of young adult lambs following exposure to chronic fetal hypoxia and antenatal Vitamin C exposure. Data presented as normalized protein expression in arbitrary units (AU) for mitochondrial abundance [MITOBIO (A); 35 kDa band on blot (G)] and OXPHOS [[complex 1 (B); 20 kDa band on blot (H)], [complex 2 (C); 30 kDa band on blot (H)], [complex 3 (D); 48 kDa band on blot (H)], [complex 4 (E); 40 kDa band on blot (H)], [complex 5 (F); 55 kDa band on blot (H)]]. Western blot images represent target protein and reference protein for Saline (S) and Vitamin C (VC) lambs in Normoxic (N) and Hypoxic groups (H). ⊗ = Outlier ± 2 SD from the treatment group mean not included in analysis for this study. Reference protein Beta actin [ß-actin Mitobiogenesis Western Blot Cocktail internal loading control; (G); 42 kDa band], and Ponceau S [total protein stain, (H)] are obtained from the same gel. Data were analyzed by Two-Way ANOVA for main effects (treatment or drug) and interaction (treatment x drug). P < 0.05 was considered statistically significant. # = effect of treatment (Normoxic vs. Hypoxic) and * = effect of drug (Saline vs. Vitamin C). All significant results presented are main effects.