Fig 1: Effect of Tβ4 on hypoxia-induced hBMVEC monolayer permeability assay and TEER analysis. (A) Permeability assays were performed using Endothelial Trans-well Permeability Assay Kit (cat. No. CB6929, Cell Biologics, Inc.), according to manufacturer’s protocols. Data expressed as the A450 absorption readings, which are considered the relative permeability. Cultured hBMVECs were pre-treated with Tβ4 (1 μg/mL) for 2 h and subjected to 24 h hypoxia. Permeability assays were performed as described above. (B) Cultured hBMVECs were pre-treated with Tβ4 (1 μg/mL) for 2 h and subjected to 24 h hypoxia. The TEER analysis was performed using Millicell® ERS-2 system. The TEER values are calculated as Ω/cm2. The permeability results are presented as the mean ± SEM (n = 3). *P < 0.05 vs. normoxia cells. #P < 0.05 vs. hypoxia cells. The TEER results are presented as the average ± SEM change of baseline TEER from at least three independent experiments consisting of at least three replicates.