Cell Counting Kit-8 (CCK-8) B34304 from Selleck Chemicals

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanySelleck Chemicals
Price $499.00Supplier Page View Company Product Page
Catalog NumberB34304
Storage3 years-20°C powder 2 years-80°C in solvent
Quantity25 mL (2500 rxns)

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Selleck Chemicals

14408 W Sylvanfield Drive

Houston, Texas TX 77014

United States

Phone: Toll Free: (877) 796-6397 (US and Canada only)
Tel: (832) 582-8158
Fax: (832) 582-8590

Email: sales@selleckchem.com

Company Profile

Website: www.selleckchem.com

(1) Machine learning algorithms develop a tumor-educated platelets-related gene signature to predict colorectal cancer prognosis and therapy responseiScienceJuly 17, 2026Yueqiong Lao, Jie Yang, Minli Hu, Jing Li, Weikang Xu, Jiahui Xu, Huan Wang, Hechenhao Jiang, Zhihao Pei, Xinyi Qiu, et al.parallel. Cell viability and colony formation assays Cell viability was measured using a CCK-8 kit (#B34304, Selleck, USA), and colony formation ability was estimated by counting the number of colonies after

(2) A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repairBioactive MaterialsJuly 1, 2026Xueheng Sun, Shangyu Xie, Ya Fang, Deteng Zhang, Wenhao Wang, Zeyue Sun, Jiaju Lu, Xiaokun Yue, Weize Kong, Dehao Fu, et al.Penicillin-streptomycin (SV30010) was obtained from HyClone Laboratories, Inc (Logan, Utah, USA). CCK-8 (B34304), and SYBR Green (B21202) were purchased by Selleck Chemicals LLC (Houston, TX, USA). Live/dead staining

(3) Snhg18 promotes hypoxic pulmonary hypertension by enhancing glycolysisRespiratory ResearchMay 18, 2026Tianyu Qu, Kai Ma, Qiang Du, Chun Zhang, Ziling Shen, Enze Wang, Zhixuan Chen, Pingsheng Chen, Yingying Liu, Ruifeng Zhanghypoxic (3% O2) or normoxic (21% O2) conditions for 24 h. Then, 10 µL Cell Counting Kit-8 reagent (Cat. B34304, Selleck) was added to each well. After incubation at 37 °C for 2 h, the absorbance at 450 nm was determined

(4) Hyperglycemia- induced innate immune tolerance involves the metabolic and epigenetic rewiring in human alveolar macrophagesFront ImmunolMay 7, 2026Jiang Wang, Na Yuan, Bin Wang, YongHui Qiu, Bo Wang, LiXin Xieviability test Cell viability was assessed using the Cell Counting Kit−8 (CCK−8) assay (Selleck, Cat# B34304, USA). Primary human AMs were seeded into 96−well plates at a density of 6 × 104 cells/well and cultured

(5) RFXANK: A Novel Immune-Related Biomarker for Hepatocellular CarcinomaGenes (Basel)March 31, 2026Taimei Qu, Lv Tian5′-CAGTGGCGTCCCTCCATTC-3′. 2.11. Cell Proliferation Assay Cell proliferation was assessed using the Cell Counting Kit-8 (B34304, Selleckchem, Houston, TX, USA). In brief, cells were inoculated into 96-well microplates at an initial

(6) An Analysis of G3BP2 in Non-Small Cell Lung CancerCancers (Basel)March 17, 2026Leela S. S. Bandi, Leah Timon, Elena O'Toole, Diarmuid O'Connor, Kristen Andersen, Bashir M. Mohamed, Siobhan Nicholson, Gerard J. Fitzmaurice, Ronan Ryan, Vincent Young, et al.further 48 h. Cellular viability was assessed using a CCK-8 assay (Selleckchem, Houston, TX, USA Cat# B34304) according to the manufacturer’s protocol. 2.12. Availability of Data and Materials The data that

(7) The segmented flavivirus ALSV-encoded nucleoprotein VP2 inhibits type I interferon production by targeting RIG-IMicrobiology SpectrumMarch 3, 2026Mingming Pan, Zhixia Song, Mengmeng Wang, Mengru Zhao, Shu Fang, Fangyu Jin, Qianqian Tan, Wenbo Xu, Lihe Che, Nan Liu, et al.after cell growth at 24 h and incubated for 12 h. Then, the cells with CCK-8 reagent (Selleck, cat#B34304) were incubated for 1 h, and the absorbance at 450 nm was measured using a microplate reader. The 50%

(8) HMOX1 drives dihydroartemisinin-sensitized ferroptosis antagonized by mitochondrial fusioniScienceJanuary 16, 2026Zi-Jie Deng, Jing Zhang, Zhang-Zhong Yang, Qing-Zhang Tuo, Peng Leisalt,Sigma-Aldrich,K1875 Rotenone,Selleck,S2348 H2O2,Macklin,H792072 FINO2,Selleck,E1244 BSO,Selleck,S9728 Erastin,Selleck,S7242 Ammonium iron(II) sulfate hexahydrate (FAS),Sigma-Aldrich,215406 Cell Counting Kit-8 (CCK-8),Selleck,B34304 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Live and Dead Cell Assay

(9) Lactate derived from cancer-associated fibroblasts promotes alternative splicing and castration resistance in prostate cancerScience AdvancesJanuary 16, 2026Diwei Zhao, Zijun Mo, Tianyou Zhang, Xinyang Cai, Zhenyu Yang, Dong Chen, Junliang Zhao, Yuanwei Li, Fangjian Zhou, Zhen Li, et al.assays For cell proliferation, CCK-8 assays were conducted following the manufacturer’s instructions (B34304, Selleck). C4-2 and LNCaP cells were plated in 96-well plates at a density of 3000 cells per well and

(10) Targeted demethylation of the BRD7 promoter based on CRISPR/dCas9 system inhibits the malignant progression of nasopharyngeal carcinomaClinical and Translational MedicineJanuary 9, 2026Jianxia Wei, Yumei Duan, Changning Xue, Lemei Zheng, Qingqing Wei, Zubing Wu, Huizhen Xin, Ting Zeng, Hongyu Deng, Songqing Fan, et al.100 µL culture medium per well were plated in a 96‐well plate. Ten microlitre CCK‐8 reagent (Cat No. B34304, Selleck) was introduced and incubated at 37°C for 2–4 h at specified designated time intervals (0,

(11) GYY4137 protects against type 2 diabetes mellitus-associated myocardial autophagy by suppressing FOXO1 signal pathwayAnimal Cells and SystemsDecember 31, 2025Gaofeng Zhu, Xiaoyong Li, Qinyuan Gao, Yuanjun Wang, Jiajie Li, Zena Huang, Yan LinPBS. Then the cells were incubated with 10 μL CCK-8 test solution (Selleck, Shanghai, China, Cat. No: B34304) and 90 μL DMEM at 37℃ for 2 h. The optical density (OD) was determined by measuring the absorbance

(12) TRIM25 degrades BRD7 protein stability through the ubiquitin proteasome pathway to promote breast cancer progression and paclitaxel resistance by activating YB1/Bcl-2 transcription axisCell Death & DiseaseNovember 28, 2025Changning Xue, Qingqing Wei, Mengna Li, Jianxia Wei, Lemei Zheng, Zubing Wu, Huizhen Xin, Yumei Duan, Songqing Fan, Wei Xiong, et al.proliferation assay, the cell viability of breast cancer cells was quantified by Cell Counting Kit-8 (CCK-8, B34304, Selleck, Houston, TX, USA). Briefly, 1000 cells/100 μl of DMEM medium were plated in each well of a

(13) Engineering Lipid-Polymer Nanoparticles for siRNA Delivery to Cancer CellsPharmaceuticals (Basel)June 10, 2025Arthur Manda, Abdulelah Alhazza, Hasan Uludağ, Hamidreza Montazeri Aliabadi1027292) were purchased from Qiagen (Germantown, MD, USA). The cell counting 8 (CCK-8) (catalogue no. B34304) kit was provided by Selleck Chemicals LLC (Houston, TX, USA). Cell culture reagents, plates, phosphate

(14) Lovastatin Targets the USP14-Survivin Axis to Suppress Triple-Negative Breast Cancer via Ubiquitin-Mediated Proteasomal DegradationCellsMay 31, 2025Li Zhou, Chanjuan Zheng, Siyu Ding, Zhiyu Wang, Yiyuan Yang, Yian Wang, Guangchun He, Shujun Fu, Xiyun Dengincluded to assess solvent effects. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, #B34304, Selleck, Houston, TX, USA) according to the manufacturer’s protocol. Three independent replicates were

(15) cIAP2-mediated IGF2BP2 ubiquitination and degradation regulate cardiomyocyte apoptosis via stabilizing m6A-modified BAX mRNA in myocardial infarctionCell Biol ToxicolMay 30, 2025Cong Wang, Jijia Liu, Xuyang Hou, Qing Guan, Huiling Zhou, Yong Luo, Wancun Jin, Fan Bai, Lijun Liu, Jian Wang, et al.seeded in 96-well plates and treated with normoxia or OGD for 6 h. After treatment, 10 μL CCK-8 (cat. #B34304, Selleck Chemicals, USA) was added and incubated for another 1 h. Then the absorbance at 450 nm was

(16) Natural compound rosmarinic acid displays anti-tumor activity in colorectal cancer cells by suppressing nuclear factor-kappa B signalingWorld Journal of Clinical OncologyMay 24, 2025Wen-Yue Liu, Han Wang, Xin Xu, Xuan Wang, Kun-Kun Han, Wen-Dao You, Yili Yang, Tao Zhangusing the Cell Counting Kit-8 (CCK-8) reagent according to the manufacturer’s instruction (Selleck, #B34304). Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction

(17) Acetyl-11-keto-β-boswellic acid alleviates hepatic metabolic dysfunction by inhibiting MGLL activityJournal of lipid researchMay 1, 2025Luan K, Fan Y, Yang Q, Yang H, Zhou Z, Huang J, She Z, Zou T, Xiong H, Mei Z.(AF20080303, purity: 98%) was purchased from Ai Fa Biotechnology Company. The Cell Counting Kit-8 (CCK8, B34304) were obtained from Selleck. Fetal bovine serum (FBS, 10,099-141) and Dulbecco′s modified Eagle′s medium

(18) Identifying Rare Germline Variants Associated with Metastatic Prostate Cancer Through an Extreme Phenotype StudymedRxivApril 30, 2025Yen-Yi Lin, Hamideh Sharifi Sharifi Noghabi, Stanislav Volik, Robert Bell, Funda Sar, Anne Haegert Haegert, Hee Chul Chung, Ladan Fazli, Htoo Zarni Zarni Oo, Mads Dauggard, et al.sensitivity to Olaparib treatment with DMSO controls on cell viability using the CCK-8 assay Selleckchem #B34304 [30]. WT and BRCA2-edited HEK293T cells were seeded in 96-well plates at 5,000 cells per well and treated

(19) The Prognostic Value and Immunomodulatory Role of Spsb2, a Novel Immune Checkpoint Molecule, in Hepatocellular CarcinomaGenes (Basel)March 17, 2025Lv Tian, Yiming Wang, Jiexin Guan, Lu Zhang, Jun FanProliferation Assay Cellular proliferation was evaluated utilizing the Cell Counting Kit-8 (CCK-8; Catalog B34304, Selleckchem). Briefly, cells were seeded in 96-well plates at a density of 4 × 103 cells/well and cultured

(20) Selective inhibition of TGF-β-induced epithelial-mesenchymal transition overcomes chemotherapy resistance in high-risk lung squamous cell carcinomaCommun BiolFebruary 1, 2025Liangdong Sun, Jue Wang, Huansha Yu, Xinsheng Zhu, Jing Zhang, Junjie Hu, Yilv Yan, Xun Zhang, Yuming Zhu, Gening Jiang, et al.proliferation Cell viability and proliferation were measured by using cell counting kit-8 (CCK-8) (Biomake, B34304). For IC50 determination, NCI-H520, HCC15 and NCI-H226 cells were plated in 96-well plates at 1 × 104

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Cell Counting Kit-8 (CCK-8) from Selleck Chemicals

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Cell Counting Kit-8 (CCK-8) from Selleck Chemicals

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